Decorin Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of ...read more
Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Decorin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Decorin
Gly17-Lys354
Accession # P28654
Specificity
Detects mouse Decorin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human Decorin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
Application Notes
ELISA Capture: Mouse Decorin Antibody (Catalog # AF1060)
ELISA Detection: Mouse Decorin Biotinylated Antibody (Catalog # BAF1060)
Reviewed Applications
Read 1 Review rated 5
using
AF1060 in the following applications:

Publications
Read Publications using
AF1060 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Decorin Antibody [Unconjugated]

  • Bone proteoglycan II
  • CSCD
  • DCN
  • decorin proteoglycan
  • Decorin
  • dermatan sulphate proteoglycans II
  • DSPG2
  • PG40
  • PGII
  • PG-II
  • PGS2
  • PG-S2
  • proteoglycan core protein
  • SLRR1B
  • SLRR1BPGS2
  • small leucine-rich protein 1B

Background

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta  Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).

  1. Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
  2. Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Decorin Antibody (AF1060)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, Western Blot.


Filter By Application
IHC
(4)
IHC-Fr
(2)
Western Blot
(2)
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Mouse
(8)
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Showing Publications 1 - 8 of 8.
Publications using AF1060 Applications Species
AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment Breast Cancer Res., 2019;21(1):31. 2019 [PMID: 30813947] (IHC, Mouse) IHC Mouse
Y Shen, V Russo, MR Zeglinski, SL Sellers, Z Wu, C Oram, S Santacruz, Y Merkulova, C Turner, K Tauh, H Zhao, T Bozin, L Bohunek, H Zeng, MA Seidman, RC Bleackley, BM McManus, E Ruoslahti, TAH Järvinen, DJ Granville Recombinant Decorin Fusion Protein Attenuates Murine Abdominal Aortic Aneurysm Formation and Rupture Sci Rep, 2017;7(1):15857. 2017 [PMID: 29158532] (IHC, Mouse) IHC Mouse
AC Daquinag, A Dadbin, B Snyder, X Wang, AA Sahin, NT Ueno, MG Kolonin Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells Mol Ther Oncolytics, 2017;6(0):1-9. 2017 [PMID: 28607949] (Western Blot, Mouse) Western Blot Mouse
Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development. Cell Death Differ, 2015;22(2):351-63. 2015 [PMID: 25342467] (IHC, Mouse) IHC Mouse
Dupuis L, Kern C Small leucine-rich proteoglycans exhibit unique spatiotemporal expression profiles during cardiac valve development. Dev Dyn, 2014;243(4):601-11. 2014 [PMID: 24272803] (IHC, Mouse) IHC Mouse
Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy. Am. J. Pathol., 2011;179(3):1179-87. 2011 [PMID: 21723246] (IHC-Fr, Mouse) IHC-Fr Mouse
Daquinag AC, Zhang Y, Amaya-Manzanares F, Simmons PJ, Kolonin MG An isoform of decorin is a resistin receptor on the surface of adipose progenitor cells. Cell Stem Cell, 2011;9(1):74-86. 2011 [PMID: 21683670] (Western Blot, Mouse) Western Blot Mouse
Magzoub M, Jin S, Verkman AS Enhanced macromolecule diffusion deep in tumors after enzymatic digestion of extracellular matrix collagen and its associated proteoglycan decorin. FASEB J., 2007;22(1):276-84. 2007 [PMID: 17761521] (IHC-Fr, Mouse) IHC-Fr Mouse

Review for Decorin Antibody (AF1060) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1060:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Decorin AF1060
Enlarge
5
reviewed by:
James Xiao
WB Mouse 09/08/2017
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Summary

ApplicationWestern Blot
Sample Testedmouse liver lysate
SpeciesMouse
LotGBG0216111

Comments

CommentsPrimary antibody was diluted in 5% milk/TBST at 1:1000. Blot was incubated in cold room overnight.

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Biotin BAF1060

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Recent Reviews

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James Xiao
09/08/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol DCN
Uniprot