Human Total MMP-3 Quantikine ELISA Kit



Product Details

Reactivity HuSpecies Glossary
Applications ELISA

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Human Total MMP-3 Quantikine ELISA Kit Summary

The Quantikine Human Total MMP-3 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure total MMP-3 (pro- and active MMP-3) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-3 and antibodies raised against the recombinant factor. Both antibodies also recognize recombinant human active MMP-3. Natural human MMP-3 showed dose-respo...nse curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-3.
Show More
Natural and recombinant human total MMP-3
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DMP300.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total MMP-3 Quantikine ELISA Kit

  • CHDS6
  • EC 3.4.24
  • EC
  • matrix metallopeptidase 3 (stromelysin 1, progelatinase)
  • matrix metalloproteinase 3 (stromelysin 1, progelatinase)
  • Matrix metalloproteinase-3
  • MGC126102
  • MGC126103
  • MMP3
  • MMP-3
  • proteoglycanase
  • SL-1
  • STMY
  • STMY1MGC126104
  • STR1
  • Stromelysin 1
  • stromelysin-1
  • transin-1


Matrix metalloproteinases (MMPs), also called matrixins, constitute a family of zinc and calcium dependent endopeptidases that function in the breakdown of extracellular matrix (ECM). They play an important role in many normal physiological processes such as embryonic development, morphogenesis, reproduction and tissue remodeling (1). They also participate in many pathological processes such as arthritis, cancer and cardiovascular disease (2). While the amounts of newly synthesized MMPs are regulated mainly at the levels of transcription, the proteolytic activities of existing MMPs are controlled through both the activation of proenzymes or zymogens and the inhibition of active enzymes by endogenous inhibitors, alpha -macroglobulins and tissue inhibitors of metalloproteinases (TIMPs). 
MMP-3 (also referred to as stromelysin-1) may be expressed in fibroblasts, chondrocytes, endothelial cells, macrophages, vascular smooth muscle cells, osteoblasts, and keratinocytes in response to appropriate stimuli (3). Various agents regulate its biosynthesis. Inflammatory cytokines such as IL-1 and TNF-alpha , epidermal growth factor, platelet-derived growth factor, phorbol and oncogenic cellular transformation are the inductive agents. In comparison, retinoic acid, glucocorticoids, estrogen, progesterone and TGF-beta suppress MMP-3 synthesis. 
MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation (4). The N-terminal pro-domain contains the cysteine switch motif conserved in MMPs that maintains MMP-3 in the latent state (5). Activation of the proenzyme results in the removal of the pro-domain. MMP-3 activation can be achieved in vitro by proteases such as itself, chyrotrypsin, neutrophil elastase and plasma kallikrein, and by mercury compounds (3). The resulting active enzyme consists of a catalytic domain with a zinc-binding motif conserved in metzincins (6,7). A short hinge peptide links the catalytic domain to the C-terminal hemopexin-like domain. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fibronectin, laminin, IGFBP-3, serpins, and IL-1 beta . The active enzyme also activates proMMP-1, -8, -9, and -13. Therefore, it is suggested that MMP-3 may participate in physiological matrix turnover and pathological destruction of the tissue. For example, MMP-3 is required for the generation of a macrophage chemoattractant in a model of herniated disc resorption (8).

Customers Who Viewed This Item Also Viewed...

Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ICC, Simple Western, WB
Species: Hu
Applications: BA
Species: Bv, Ca, Hu, Mu, Po, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for MMP-3 (DMP300)(50)

We have publications tested in 1 confirmed species: Human.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using DMP300 Applications Species
TW Kim, AG Kim, KH Lee, MH Hwang, H Choi Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions International Journal of Molecular Sciences, 2022;23(17):. 2022 [PMID: 36077518] (Human) Human
JH Shin, S Park, H Cho, JH Kim, H Choi Adipokine human Resistin promotes obesity-associated inflammatory intervertebral disc degeneration via pro-inflammatory cytokine cascade activation Scientific Reports, 2022;12(1):8936. 2022 [PMID: 35624126] (Human) Human
M Dreiner, T Munk, F Zaucke, AM Liphardt, A Niehoff Relationship between different serum cartilage biomarkers in the acute response to running and jumping in healthy male individuals Scientific Reports, 2022;12(1):6434. 2022 [PMID: 35440750] (Human) Human
BG Jung, B Samten, K Dean, RJ Wallace, BA Brown-Elli, T Tucker, S Idell, JV Philley, R Vankayalap Early IL-17A production helps establish Mycobacterium intracellulare infection in mice PloS Pathogens, 2022;18(4):e1010454. 2022 [PMID: 35363832] (Human) Human
J Jarecki, T Ma?ecka-Ma, E Kosior-Jar, W Widuchowsk, P Krasowski, M Gutbier, M Dobrzy?ski, T Blicharski Concentration of Selected Metalloproteinases and Osteocalcin in the Serum and Synovial Fluid of Obese Women with Advanced Knee Osteoarthritis International Journal of Environmental Research and Public Health, 2022;19(6):. 2022 [PMID: 35329213] (Human) Human
AS Sørensen, MN Andersen, K Juul-Madse, AD Broksø, C Skejø, H Schmidt, T Vorup-Jens, TW Kragstrup Tumor necrosis factor alpha neutralization attenuates immune checkpoint inhibitor-induced activation of intermediate monocytes in synovial fluid mononuclear cells from patients with inflammatory arthritis Arthritis Research & Therapy, 2022;24(1):43. 2022 [PMID: 35164829] (Human) Human
J Rademacher, M Siderius, L Gellert, FR Wink, M Verba, F Maas, LM Tietz, D Poddubnyy, A Spoorenber, S Arends Baseline serum biomarkers of inflammation, bone turnover and adipokines predict spinal radiographic progression in ankylosing spondylitis patients on TNF inhibitor therapy Seminars in arthritis and rheumatism, 2022;53(0):151974. 2022 [PMID: 35150984] (Human) Human
J Kudelski, G M?ynarczyk, M Gudowska-S, B Mroczko, B Darewicz, M Bruczko-Go, K Sobolewski, L Romanowicz Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma Journal of Clinical Medicine, 2021;10(16):. 2021 [PMID: 34441979] (Human) Human
HK Pak, YW Kim, B Nam, AN Lee, J Roh, M Gil, C Liu, YS Chung, CS Park The Migration of Human Follicular Dendritic Cell-Like Cell Is Facilitated by Matrix Metalloproteinase 3 Expression That Is Mediated through TNFalpha-ERK1/2-AP1 Signaling Journal of Immunology Research, 2021;2021(0):8483938. 2021 [PMID: 34222497] (Human) Human
K Chihara, N Hattori, N Ichikawa, T Matsuda, T Saito Re-evaluation of serum leptin and adiponectin concentrations normalized by body fat mass in patients with rheumatoid arthritis Sci Rep, 2020;10(1):15932. 2020 [PMID: 32985609] (Human) Human
Show All 50 Publications.

Reviews for MMP-3 (DMP300) (0)

There are no reviews for MMP-3 (DMP300). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MMP-3 (DMP300) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MMP-3 Products

Bioinformatics Tool for MMP-3 (DMP300)

Discover related pathways, diseases and genes to MMP-3 (DMP300). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MMP-3 (DMP300)

Discover more about diseases related to MMP-3 (DMP300).

Pathways for MMP-3 (DMP300)

View related products by pathway.

PTMs for MMP-3 (DMP300)

Learn more about PTMs related to MMP-3 (DMP300).

Blogs on MMP-3.

MMP3 - a potential target for arthritis therapies
Matrix metalloproteinases (MMPs) are responsible for the degradation of extracellular matrix proteins. MMPs are essential for tissue remodeling during normal processes such as embryonic development as well as pathological conditions such as arthri...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human Total MMP-3 Quantikine ELISA Kit and receive a gift card or discount.


Gene Symbol MMP3