SPARC Antibody [Unconjugated]

Images

 
Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
SPARC/Osteonectin was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse SPARC/Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 1.7 µg/mL overnight at 4 °C. ...read more
SPARC was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 5 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SPARC Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin
Ala18-Ile302
Accession # P07214
Specificity
Detects mouse SPARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human SPARC is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPARC
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
AF942 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPARC Antibody [Unconjugated]

  • Basement-membrane protein 40
  • BM-40
  • ONcysteine-rich protein
  • Osteonectin
  • Secreted protein acidic and rich in cysteine
  • secreted protein, acidic, cysteine-rich (osteonectin)
  • SPARC

Background

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1-5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1-5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets, and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3-5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3-5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92%, and 83% aa identity with rat, human, dog, cow, and chick SPARC, respectively.

  1. Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
  2. McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
  3. Sage, H. et al. (1989) J. Cell Biol. 109:341.
  4. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  5. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
  6. Hohenester, E. et al. (1997) EMBO J. 16:3778.
  7. Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
  8. Delany, A.M. et al. (2003) Endocrinology 144:2588.
  9. Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
  10. Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
  11. Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPARC Antibody (AF942)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
ICC
(1)
IHC
(3)
IHC-Fr
(1)
Western Blot
(11)
All Applications
Filter By Species
Human
(1)
Mouse
(15)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF942 Applications Species
T Shoneye, AT Orrego, R Jarvis, Y Men, MSR Chiang, Y Yang Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development Front Neurosci, 2020;14(0):435. 2020 [PMID: 32457572] (IHC, Mouse) IHC Mouse
J Falero-Per, N Sheibani, CM Sorenson Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells PLoS ONE, 2020;15(5):e0232779. 2020 [PMID: 32365083] (Western Blot, Mouse) Western Blot Mouse
I Ramu, SM Buchholz, MS Patzak, RG Goetze, SK Singh, FM Richards, DI Jodrell, B Sipos, P Ströbel, V Ellenriede, E Hessmann, A Neesse SPARC dependent collagen deposition and gemcitabine delivery in a genetically engineered mouse model of pancreas cancer EBioMedicine, 2019;0(0):. 2019 [PMID: 31597597]
S Deckx, DM Johnson, M Rienks, P Carai, E Van Deel, J Van der Ve, KR Sipido, S Heymans, AP Papageorgi Extracellular SPARC increases cardiomyocyte contraction during health and disease PLoS ONE, 2019;14(4):e0209534. 2019 [PMID: 30933983] (Western Blot, Mouse) Western Blot Mouse
J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells PLoS ONE, 2018;13(10):e0206756. 2018 [PMID: 30372497] (Western Blot, Mouse) Western Blot Mouse
EV Jones, Y Bernardine, JG Zarruk, S Chierzi, KK Murai SPARC and GluA1-Containing AMPA Receptors Promote Neuronal Health Following CNS Injury Front Cell Neurosci, 2018;12(0):22. 2018 [PMID: 29449802] (Western Blot, Mouse) Western Blot Mouse
L Morel, MSR Chiang, H Higashimor, T Shoneye, LK Iyer, J Yelick, A Tai, Y Yang Molecular and Functional Properties of Regional Astrocytes in the Adult Brain J. Neurosci., 2017;0(0):. 2017 [PMID: 28821665] (IHC, Mouse) IHC Mouse
Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model Oncotarget, 2016;7(21):31097-110. 2016 [PMID: 27105498] (Western Blot, Mouse) Western Blot Mouse
Tseng C, Kolonin M Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity. Stem Cells, 2015;34(1):174-90. 2015 [PMID: 26381424] (Western Blot, Mouse) Western Blot Mouse
Mathsyaraja H, Thies K, Taffany D, Deighan C, Liu T, Yu L, Fernandez S, Shapiro C, Otero J, Timmers C, Lustberg M, Chalmers J, Leone G, Ostrowski M CSF1-ETS2-induced microRNA in myeloid cells promote metastatic tumor growth. Oncogene, 2015;34(28):3651-61. 2015 [PMID: 25241894] (Western Blot, Mouse) Western Blot Mouse
Show All 17 Publications.

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Biotin BAF942

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Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway.  Apart from its role in gl...  Read full blog post.

GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)
GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin...  Read full blog post.

FLICE, FLICE, baby
Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.

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Bioinformatics

Gene Symbol SPARC
Uniprot