SPARC Antibody [Unconjugated]


Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # more
SPARC/Osteonectin was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse SPARC/Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 1.7 µg/mL overnight at 4 °C. more
SPARC was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 5 µg/mL for 3 hours at room temperature. Cells more
Balb/C-3T3 mouse fibroblast cell line was stained with Goat Anti-Mouse SPARC Polyclonal Antibody (Catalog # AF942, filled histogram) or Goat IgG control antibody (AB-108-C, open histogram), followed by more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC, CyTOF-ready, Flow, ICC/IF

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SPARC Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin
Accession # P07214
Detects mouse SPARC in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.2 ug/mL
Read Publications using
AF942 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPARC Antibody [Unconjugated]

  • Basement-membrane protein 40
  • BM-40
  • ONcysteine-rich protein
  • Osteonectin
  • Secreted protein acidic and rich in cysteine
  • secreted protein, acidic, cysteine-rich (osteonectin)


SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1-5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1-5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets, and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3-5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3-5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92%, and 83% aa identity with rat, human, dog, cow, and chick SPARC, respectively.

  1. Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
  2. McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
  3. Sage, H. et al. (1989) J. Cell Biol. 109:341.
  4. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  5. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
  6. Hohenester, E. et al. (1997) EMBO J. 16:3778.
  7. Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
  8. Delany, A.M. et al. (2003) Endocrinology 144:2588.
  9. Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
  10. Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
  11. Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPARC Antibody (AF942)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using AF942 Applications Species
YS Song, N Jamali, CM Sorenson, N Sheibani Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial Cells Cells, 2023-01-16;12(2):. 2023-01-16 [PMID: 36672270] (Western Blot, Mouse) Western Blot Mouse
WW MacDonald, SS Swaminatha, JY Heo, A Castillejo, J Hsueh, BJ Liu, D Jo, A Du, H Lee, MH Kang, DJ Rhee Effect of SPARC Suppression in Mice, Perfused Human Anterior Segments, and Trabecular Meshwork Cells Investigative Ophthalmology & Visual Science, 2022-06-01;63(6):8. 2022-06-01 [PMID: 35671048] (IHC, Mouse) IHC Mouse
A Ghanemi, A Melouane, M Yoshioka, J St-Amand Secreted Protein Acidic and Rich in Cysteine (Sparc) KO Leads to an Accelerated Ageing Phenotype Which Is Improved by Exercise Whereas SPARC Overexpression Mimics Exercise Effects in Mice Metabolites, 2022-01-28;12(2):. 2022-01-28 [PMID: 35208200] (Western Blot, Mouse) Western Blot Mouse
Y Xie, AT Kuan, W Wang, ZT Herbert, O Mosto, O Olukoya, M Adam, S Vu, M Kim, D Tran, N Gómez, C Charpentie, I Sorour, TE Lacey, MY Tolstoruko, BL Sabatini, WA Lee, CC Harwell Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development Cell Reports, 2022-02-22;38(8):110416. 2022-02-22 [PMID: 35196485] (IHC, Mouse) IHC Mouse
RL Kosinsky, D Saul, C Ammer-Herr, WA Faubion, A Neesse, SA Johnsen USP22 Suppresses SPARC Expression in Acute Colitis and Inflammation-Associated Colorectal Cancer Cancers, 2021-04-10;13(8):. 2021-04-10 [PMID: 33920268] (IHC, Mouse) IHC Mouse
T Shoneye, AT Orrego, R Jarvis, Y Men, MSR Chiang, Y Yang Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development Front Neurosci, 2020-05-08;14(0):435. 2020-05-08 [PMID: 32457572] (IHC, Mouse) IHC Mouse
J Falero-Per, N Sheibani, CM Sorenson Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells PLoS ONE, 2020-05-04;15(5):e0232779. 2020-05-04 [PMID: 32365083] (Western Blot, Mouse) Western Blot Mouse
SD Praktiknjo, B Obermayer, Q Zhu, L Fang, H Liu, H Quinn, M Stoeckius, C Kocks, W Birchmeier, N Rajewsky Tracing tumorigenesis in a solid tumor model at single-cell resolution Nat Commun, 2020-02-20;11(1):991. 2020-02-20 [PMID: 32080185] (IHC, Mouse) IHC Mouse
I Ramu, SM Buchholz, MS Patzak, RG Goetze, SK Singh, FM Richards, DI Jodrell, B Sipos, P Ströbel, V Ellenriede, E Hessmann, A Neesse SPARC dependent collagen deposition and gemcitabine delivery in a genetically engineered mouse model of pancreas cancer EBioMedicine, 2019-10-06;0(0):. 2019-10-06 [PMID: 31597597] (IHC-P, Western Blot, Mouse) IHC-P, Western Blot Mouse
S Deckx, DM Johnson, M Rienks, P Carai, E Van Deel, J Van der Ve, KR Sipido, S Heymans, AP Papageorgi Extracellular SPARC increases cardiomyocyte contraction during health and disease PLoS ONE, 2019-04-01;14(4):e0209534. 2019-04-01 [PMID: 30933983] (ICC, Western Blot, Mouse) ICC, Western Blot Mouse
Show All 23 Publications.

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Gene Symbol SPARC