Collagen IV Antibody

Immunocytochemistry/Immunofluorescence: Collagen IV Antibody [NB120-6586] - Immunofluorescence staining of MDA-MB-231 cells for collagen IV after 7 days of culture in 6 vs. 2.5mgml^-1. Representative images of n=2 biological replicates. Scale bar 100um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29162797/) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in various paraffin embedded human tissues. Image courtesy of product review submitted by Daniel Pirici.

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Collagen IV antibody at 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (left) with strong red staining observed in glomeruli and liver (right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 100C for 20 minutes for antigen retrieval.

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in paraffin embedded mouse cervix tissue using anti-Collagen IV antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis using the Biotin conjugate of NB120-6586. Staining of Collagen IV (red) and Insulin (green) in mouse pancreas. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586] - Mouse liver tissue. Collagen IV antibody at 1:200 dilution. Overnight incubation, no permeabilization. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586] - Mouse brain tissue. Antibody at 1:500. IHC-Fr image submitted by a verified customer review.

Knockout Validated: Collagen IV Antibody [NB120-6586] - Collagen IV was detected in immersion fixed THP-1 (positive) but not THP-1 KO (negative) cells using 10 ug/mL Rabbit Anti-Human/Bovine Polyclonal Antibody (Catalog # NB120-6586) for 3 hours at room temperature. Cells were stained with the NorthernLights(TM) 557-conjugated Donkey anti-Rabbit IgG Secondary Antibody [NL557] (red; Catalog # NL004) and counterstained with DAPI (blue).

• Reactivity: Hu, Mu, Rt, Bv, Fe
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, KO, SR Microscopy
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Collagen IV Antibody Summary

• Immunogen: Collagen IV from human and bovine placenta (Uniprot: P02462)
• Localization: Secreted and Cell surface (Potential).
• Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:100
  • Knockout Validated
  • Super-Resolution Microscopy
  • Western Blot 1:1000-1:10000
• Application Notes: This product has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
  Use in Immunohistochemistry Free-Floating reported in scientific literature (PMID: 32245949).

Reactivity Notes

This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Rat reactivity reported in scientific literature (PMID: 16774905)

Feline reactivity reported in scientific literature (PMID: 33091431).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Collagen IV Antibody

• Collagen IV

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen IV Antibody (NB120-6586)(29)

Publications using NB120-6586

• Abrahamson EE, Padera RF, Davies J et al. The flutemetamol analogue cyano-flutemetamol detects myocardial AL and ATTR amyloid deposits: a post-mortem histofluorescence analysis Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis 2022-11-21 [PMID: 36411500]
• Fujimura Y, Watanabe M, Ohno K, Kobayashi Y Subepidermal blisters are healed by wedge-shaped keratinocytes of hair follicle origin bioRxiv 2020-01-01 (ICC/IF, Mouse)
• Peng M, Curry SM, Liu Y et al. The ex vivo human translaminar autonomous system to study spaceflight associated neuro-ocular syndrome pathogenesis NPJ microgravity 2022-10-28 [PMID: 36307487] (IHC-P, Human)
• Caporarello N, Lee J, Pham TX et al. Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis Nature communications 2022-07-25 [PMID: 35879310] (IHC-P, Mouse)
• Lenti, E;Genovese, L;Bianchessi, S;Maurizio, A;Sain, SB;di Lillo, A;Mattavelli, G;Harel, I;Bernassola, F;Hehlgans, T;Pfeffer, K;Crosti, M;Abrignani, S;Evans, SM;Sitia, G;GuimarAes-Camboa, N;Russo, V;van de Pavert, SA;Garcia-Manteiga, JM;Brendolan, A; Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors Immunity [PMID: 35358427]
• Xie X, Li J, Liu P et al. Chimeric Fusion between Clostridium ramosum IgA Protease and IgG Fc Provides Long-lasting Clearance of IgA deposits in Mouse Models of IgA Nephropathy Journal of the American Society of Nephrology : JASN 2022-02-16 [PMID: 35172987] (IHC-Fr, Mouse)
• Robertson C, Sebastian A, Hinckley A et al. Extracellular matrix modulates T cell clearance of malignant cells in vitro Biomaterials 2022-03-01 [PMID: 35121359] (ICC/IF, Mouse)
• Wohlfeil SA, Hafele V, Dietsch B et al. Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment Journal of translational medicine 2022-02-02 [PMID: 35109875]
• Acevedo Ds CCL2/CCR2 chemokine signaling modulates ductal breast cancer progression by enhancing glycolysis and c-MET activation Thesis 2021-01-01 (IHC)
• Ishii H, Kaneko S, Yanai K et al. MicroRNA Expression Profiling in Diabetic Kidney Disease Translational research : the journal of laboratory and clinical medicine 2021-06-06 [PMID: 34102327] (IHC, Mouse)
• Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline)
• Wang S, Chen P, Sung Y et al. Effect of COL4A1 Expression on the Survival of Neoadjuvant Chemotherapy Breast Cancer Patients Journal of Oncology 2020-05-15 [PMID: 32565804] (IHC, Human)
• Leng S, Pignatti E, Khetani RS et al. Beta-Catenin and FGFR2 regulate postnatal rosette-based adrenocortical morphogenesis Nat Commun 2020-04-03 [PMID: 32245949] (IHC-FrFl, Mouse)
• Li W, Zhan M, Quan YY et al. Modulating the tumor immune microenvironment with sunitinib malate supports the rationale for combined treatment with immunotherapy Int. Immunopharmacol. 2020-02-17 [PMID: 32078941] (ICC/IF, Mouse)
• Willemse J, Verstegen M, Vermeulen A et al. Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion Materials Science and Engineering: C 2019-09-12 [PMID: 31923991] (IHC, IHC-P, Human)
• Mehta N, Gava AL, Zhang D et al. Follistatin Protects Against Glomerular Mesangial Cell Apoptosis and Oxidative Stress to Ameliorate Chronic Kidney Disease Antioxid. Redox Signal. 2019-07-03 [PMID: 31184201] (WB)
• MacDonald JA, Takai Y, Ishihara O et al. Extracellular matrix signaling activates differentiation of adult ovary derived oogonial stem cells ina species-specific manner Fertil. Steril. 2019-04-01 [PMID: 30871765] (IHC-P, Mouse)
• Trasino SE, Tang XH, Shevchuk MM et al. Amelioration of Diabetic Nephropathy Using a Retinoic Acid Receptor beta 2 Agonist J Pharmacol Exp Ther. 2018-10-01 [PMID: 30054312] (IHC-P, Mouse)
• RUger Beate M, Buchacher Tanja, Giurea Alexander et al. Vascular Morphogenesis in the Context of Inflammation: Self-Organization in a Fibrin-Based 3D Culture System. Journal of the American Heart Association 2018-06-05 [PMID: 29922175] (SR Microscopy, Human)
• Winter C, Silvestre-Roig C et al. Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis. Cell Metab 2018-03-07 [PMID: 29861387] (ICC/IF, Mouse)
• Nikolaou S, Hadjikypri X, Ioannou G et al. Functional and phenotypic distinction of the first two trophoblast subdivisions and identification of the border between them during early postimplantation: A prerequisite for understanding early patterning during placentogenesis. Biochem Biophys Res Commun 2018-01-29 [PMID: 29305264] (IHC-P)
• Velez DO, Tsui B, Goshia T et al. 3D collagen architecture induces a conserved migratory and transcriptional response linked to vasculogenic mimicry Nat Commun 2017-11-21 [PMID: 29162797] (ICC/IF)
• Lin EY, Bayarsengee U, Wang CC et al. The natural compound 2,3,5,4'-tetrahydroxystilbene-2-O-b-d glucoside protects against adriamycin-induced nephropathy through activating the Nrf2-Keap1 antioxidant pathway. Environ. Toxicol. 2017-10-24 [PMID: 29064158] (Mouse)
• Bracaglia LG, Messina M, Winston S et al. 3D Printed Pericardium Hydrogels To Promote Wound Healing in Vascular Applications. Biomacromolecules. 2017-10-16 [PMID: 28976740] (Rat)
• Liguori R, Incensi A, de Pasqua S et al. Skin globotriaosylceramide 3 deposits are specific to Fabry disease with classical mutations and associated with small fibre neuropathy PLoS ONE 2017-07-03 [PMID: 28672034] (IHC-Fr, Human)
• Root-Bernstein R Rethinking Molecular Mimicry in Rheumatic Heart Disease and Autoimmune Myocarditis: Laminin, Collagen IV, CAR, and B1AR as Initial Targets of Disease. Pediatric Cardiology 2014-08-19 [PMID: 25191648]
• Mogoanta L, Ciurea M, Pirici I et al. Different dynamics of aquaporin 4 and glutamate transporter 1 distribution in the perineuronal and perivascular compartments during ischemic stroke. Brain Pathol. 2014-02-27 [PMID: 24571080] (IHC-P, Human)
• Pirici D, Pirici I, Mogoanta L et al. Matrix metalloproteinase-9 expression in the nuclear compartment of neurons and glial cells in aging and stroke Neuropathology 2012-10-01 [PMID: 22151540] (IHC-P, Rat)
• Xu, H, Zeng, L, Peng, H, Chen, S, Jones, J, Chew, T-L, Sadeghi, M M, Kanwar, Y S, Danesh, F R. HMG-CoA reductase inhibitor simvastatin mitigates VEGF-induced quot;inside-outquot; signaling to extracellular matrix by preventing RhoA activation, . 291, F995-1004. 2006-01-01 [PMID: 16774905]

Reviews for Collagen IV Antibody (NB120-6586) (7)

Average Rating: 4.9

(Based on 7 reviews)

We have 7 reviews tested in 3 species: Human, Mouse, Feline.

reviewed by: yasuy ohguch IHC-P Human 10/09/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: blade
• Species: Human
• Lot: 40173

reviewed by: Verified Customer IHC-Fr Mouse 09/10/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: 35822

Comments

• Comments: 1:500

reviewed by: Mozhdeh Sojoodi IHC-Fr Mouse 08/09/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Liver
• Species: Mouse
• Lot: 40549

reviewed by: David Sun IHC-Fr Feline 02/18/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Connective tissue
• Species: Feline
• Lot: 37798

reviewed by: Sanghyuk Chung IHC-P Mouse 04/27/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse cervix
• Species: Mouse

reviewed by: Daniel Pirici IHC-P 09/15/2012

Summary

• Application: Immunohistochemistry-Paraffin

reviewed by: Daniel Pirici IHC-P Human 09/15/2012

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human tissue
• Species: Human

Bioinformatics

• Gene Symbol: COL4A1
• Uniprot:
  • P02462(Human)