Collagen I Antibody

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Rat colon tissue stained with Collagen I antibody (red) and Hoechst (blue). IHC-P image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Myofibroblast differentiation requires CD271/NGF activation. Representative alphaSMA (red) and COLLAGEN 1 (red) immunostaining with DAPI (blue) in non-treated (control), TGFbeta-treated cells with or without gamma-secretase inhibitor. Scale bars: 50 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY licence.

Western Blot: Collagen I Antibody [NB600-408] - Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.

Western Blot: Collagen I Antibody [NB600-408] - Representative Western blot image for Collagen I, Collagen 2 and GAPDH. N/group = 8. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.12367) licensed under a CC-BY licence.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Macro- and micro-architecture of human adipose tissue lobule. Representative immunostaining of the human AT lobule with Collagen I (COL1), CD34, and DAPI. The position of the septa is underlined, scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Primary human cardiac fibroblast cells were stained with anti-Collagen I antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf serum. ICC/IF image submitted by a verified customer review.

Western Blot: Collagen I Antibody [NB600-408] - Cell lysate from human trabecular meshwork. Antibody at 1:1.000. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Staining of human HaCaT-ras A5-RT3 (SCC) frozen tumor sections. Image provided by Dr. Wa'el Al Rawashdeh of RWTH Aachen University.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Patient-derived CAF and NF CDMs are architecturally and functionally distinct. Representative western blots showing Collagen I (red) and fibronectin (blue) staining of patient #1 and #3 NF and CAF CDM. Scale bar, 20 um. (f) Representative SEM images of TIFF, Patient #1 NF and CAF CDM. Scale bar, 5 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms12237) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of Collagen I Antibody on alcohol-fixed rat lung tissue. Primary antibody dilution 1:200. Image from verified customer review.

Western Blot: Collagen I Antibody [NB600-408] - Analysis in porcine burn wound lysate using anti-Collagen I alpha 1 antibody. WB image submitted by a verified customer review.

Western Blot: Collagen I Antibody [NB600-408] - Detection of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2-2 ug/10 mL in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% non-fat milk. Detection occurred by incubation with a HRP conjugated secondary antibody at 1 ug/10 mL. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Balb/3T3 mouse embryonic fibroblast cell line stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Mouse pancreas tissue stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Human lung tissue section stained with Collagen I antibody at 1:400.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - FFPE right lobe of the liver tissue section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Analysis of FFPE human lung. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin at pH 6. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin tissue at pH9. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Imaging of rat calvarial defect bone. IHC-P image submitted by a verified customer review.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Immunohistological staining of EG treated rat kidneys for Collagen. Magnification X40, Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0185009), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Rb
• Applications: WB, ELISA, FLISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PAGE, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Collagen I Antibody Summary

• Immunogen: Collagen I from human and bovine placenta (Uniprot: P02452)
• Localization: Extracellular matrix
• Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:50000
  • Fluorophore-linked immunosorbent assay 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Knockdown Validated
  • Microarray
  • SDS-Page
  • Western Blot 1:1000-1:10000
• Application Notes: This product has been tested by dot blot and IHC and are useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, Immunofluorescence, FLOW, and western blotting for highly sensitive qualitative analysis.
  
  Use in SDS-Page reported in scientific literature (PMID: 30914477).

Reactivity Notes

This antibody reacts with human, bovine, and most mammalian Type I collagens with negligible cross-reactivity with Type II, III, IV, V or VI collagens
Use in Mouse reported in scientific literature (PMID: 33758176).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Collagen I Antibody

• Alpha-1 type I collagen
• alpha1(I) procollagen
• CAFYD
• COL1A1
• Collagen 1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain preproprotein
• collagen alpha-1(I) chain
• Collagen I
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• EDSARTH1
• EDSC
• OI1
• OI2
• OI3
• OI4
• pro-alpha-1 collagen type 1
• type I proalpha 1
• Type I Procollagen Alpha 1 Chain

Background

Collagen I is one of the most abundant extracellular matrix (ECM) proteins in mammals and is an important structural component in connective tissues, bones, teeth, skin, heart, and lungs (1, 2). Collagen I is commonly used for biomedical purposes including providing an ECM microenvironment for cell attachment, proliferation, and mesenchymal stem cell growth and differentiation to the osteogenic lineage (1, 3, 4). Furthermore, type I Collagen hydrogels are a promising scaffold for engineering tissues and tumors (4). Collagen type I can be extracted from numerous sources including mammals, most commonly bovine and porcine, fish, amphibians, and birds (1, 4). Structurally, Collagen I is a heterotrimer comprised of two alpha-1 chains and one alpha-2 chain that together forms a triple helix (1, 3, 5). Each alpha chain is primarily formed by glycine, proline, and hydroxyproline repeats with the alpha-1 chains having a theoretical molecular weight of 139 kDa and 129 kDa for the alpha-2 chain (3). The dimeric form of Collagen I has a theoretical molecular weight of ~270 kDa while the trimeric form is >400 kDa (3, 5).

A variety of disease pathologies have been associated with mutations in or disruptions in collagen type I. Osteogenesis imperfecta, also known as brittle bone disease, is caused by autosomal dominant mutations in Collagen type I encoding genes and characterized by fragile bones and skeletal deformities (6). Ehlers-Danlos syndromes (EDS) is another disorder that affects soft connective tissue and its various clinical subtypes are associated with different mutations in Collagen I (7).

Alternative names for Collagen I includes alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, and type I procollagen alpha 1 chain.

References

1. Chowdhury, S. R., Mh Busra, M. F., Lokanathan, Y., Ng, M. H., Law, J. X., Cletus, U. C., & Binti Haji Idrus, R. (2018). Collagen Type I: A Versatile Biomaterial. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-13-0947-2_21

2. Kisling, A., Lust, R. M., & Katwa, L. C. (2019). What is the role of peptide fragments of collagen I and IV in health and disease?. Life sciences. https://doi.org/10.1016/j.lfs.2019.04.042

3. Kwon, D., Kang, G. S., Han, D. K., Park, K., Kim, J. H., & Lee, S. H. (2014). Establishment and characterization of human engineered cells stably expressing large extracellular matrix proteins. Archives of pharmacal research. https://doi.org/10.1007/s12272-013-0294-0

4. Antoine, E. E., Vlachos, P. P., & Rylander, M. N. (2014). Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport. Tissue engineering. Part B, Reviews. https://doi.org/10.1089/ten.TEB.2014.0086

5. Leon-Lopez, A., Morales-Penaloza, A., Martinez-Juarez, V. M., Vargas-Torres, A., Zeugolis, D. I., & Aguirre-alvarez, G. (2019). Hydrolyzed Collagen-Sources and Applications. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules24224031

6. Marini, J. C., Forlino, A., Bachinger, H. P., Bishop, N. J., Byers, P. H., Paepe, A., Fassier, F., Fratzl-Zelman, N., Kozloff, K. M., Krakow, D., Montpetit, K., & Semler, O. (2017). Osteogenesis imperfecta. Nature reviews. Disease primers. https://doi.org/10.1038/nrdp.2017.52

7. Callewaert, B., Malfait, F., Loeys, B., & De Paepe, A. (2008). Ehlers-Danlos syndromes and Marfan syndrome. Best practice & research. Clinical rheumatology, 22(1), 165-189. https://doi.org/10.1016/j.berh.2007.12.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen I Antibody (NB600-408)(237)

Publications using NB600-408

• Lewis A, SAnchez S, Berti G et al. Small-molecule Wnt inhibitors are a potential novel therapy for intestinal fibrosis in Crohns disease Clinical science (London, England : 1979) Oct 14 2022 [PMID: 36156078] (ICC/IF, Human)
• Paul K, Darzi S, Del Borgo M et al. Vaginal delivery of tissue engineered endometrial mesenchymal stem/stromal cells in an aloe vera-alginate hydrogel alleviates maternal simulated birth injury Applied Materials Today Mar 1 2021 (ICC/IF, Human)
• Mi YB, Liu XH, Wang WS et al. ER-phagy Is Involved in the Degradation of Collagen I by IL-1B in Human Amnion in Parturition Journal of immunology (Baltimore, Md. : 1950) Oct 26 2022 [PMID: 36288908] (WB, Human)
• Gong H, Chen L, He Y et al. Pleural thickening induced by Glaesserella parasuis infection was linked to increased collagen and elastin Frontiers in cellular and infection microbiology Aug 11 2022 [PMID: 36034702] (IHC-P, Porcine)
• Zhou YH, Guo Y, Zhu JY et al. Spheroid co-culture of BMSCs with osteocytes yields ring-shaped bone-like tissue that enhances alveolar bone regeneration Scientific reports Aug 27 2022 [PMID: 36030312] (IHC-P, Mouse)
  
  Details:
  IHC-P 1:100
• Caporarello N, Lee J, Pham TX et al. Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis Nature communications Jul 25 2022 [PMID: 35879310] (WB, Human)
• Hadjisavva R, Anastasiou O, Ioannou PS et al. Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition Cell reports Jul 19 2022 [PMID: 35858563] (ICC, Human)
• Li X, Yong T, Wei Z et al. Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblasts Nature communications May 19 2022 [PMID: 35589680] (IHC, Human, Mouse)
• Xue W, Yu SY, Kuss M et al. 3D bioprinted white adipose model forin vitrostudy of cancer-associated cachexia induced adipose tissue remodeling Biofabrication May 26 2022 [PMID: 35504266]
• Park HH, Kim YM, Anh Hong LT et al. Dual-functional hydrogel system for spinal cord regeneration with sustained release of arylsulfatase B alleviates fibrotic microenvironment and promotes axonal regeneration Biomaterials May 1 2022 [PMID: 35461098] (IHC, Rat)
• Pothiawala A, Sahbazoglu BE, Ang BK et al. GDF5 + chondroprogenitors derived from human pluripotent stem cells preferentially form permanent chondrocytes Development (Cambridge, England) Apr 22 2022 [PMID: 35451016]
• Aidos L, Modina SC, Millar VRH et al. Meniscus Matrix Structural and Biomechanical Evaluation: Age-Dependent Properties in a Swine Model Bioengineering (Basel, Switzerland) Mar 15 2022 [PMID: 35324808] (WB, IHC, Porcine)
• Trott AJ, Greenwell BJ, Karhadkar TR et al. Lack of food intake during shift work alters the heart transcriptome and leads to cardiac tissue fibrosis and inflammation in rats BMC biology Mar 3 2022 [PMID: 35236346] (IHC, Rat)
• Li C, Yang M, Qu Z et al. Effect of electroacupuncture on the degradation of collagen in pelvic floor supporting tissue of stress urinary incontinence rats International urogynecology journal Feb 28 2022 [PMID: 35226143] (WB, Rat)
• Rusu-Nastase E, Lupan A, Marinescu C et al. MiR-29a Increase in Aging May Function as a Compensatory Mechanism Against Cardiac Fibrosis Through SERPINH1 Downregulation Frontiers in Cardiovascular Medicine Jan 18 2022 [PMID: 35118144] (ICC/IF, Mouse)
• Srinivasan MP, Bhopale KK, Caracheo AA et al. Exposure to binge ethanol and fatty acid ethyl esters exacerbates chronic ethanol-induced pancreatic injury in hepatic alcohol dehydrogenase deficient deer mice American journal of physiology. Gastrointestinal and liver physiology Jan 5 2022 [PMID: 34984929] (WB, Mouse)
• Wang R, Kairen C, Li L et al. Overexpression of NDUFV1 alleviates renal damage by improving mitochondrial function in unilateral ureteral obstruction model mice Cell biology international Dec 22 2021 [PMID: 34936716]
• Keller CR, Hu Y, Ruud KF Et al. Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells Cancers Nov 22 2021 [PMID: 34831010] (ICC/IF, Human)
• Kasetti RB, Maddineni P, Kodati B Et al. Astragaloside IV Attenuates Ocular Hypertension in a Mouse Model of TGF beta 2 Induced Primary Open Angle Glaucoma International journal of molecular sciences Nov 19 2021 [PMID: 34830390] (ICC/IF, Human)
• de Sousa BM, Correia CR, Ferreira JAF Et al. Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants NPJ Regenerative medicine Nov 23 2021 [PMID: 34815414] (WB, Mouse)
• Lee J, Ung A, Kim H Et al. Engineering liver microtissues to study the fusion of HepG2 with mesenchymal stem cells and invasive potential of fused cells Biofabrication Nov 30 2021 [PMID: 34740205]
• Miura Y, Lam M, Bourke JE, Kanazawa S Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia Life science alliance Jan 1 2022 [PMID: 34728556] (IHC-P, Mouse)
• Huang LT, Chou HC, Chen CM. Inhibition of FABP4 attenuates hyperoxia-induced lung injury and fibrosis via inhibiting TGF- beta signaling in neonatal rats Journal of cellular physiology Oct 28 2021 [PMID: 34708870] (Rat)
• Maddineni P, Kasetti RB, Kodati B et al. Sodium 4-Phenylbutyrate Reduces Ocular Hypertension by Degrading Extracellular Matrix Deposition via Activation of MMP9 International journal of molecular sciences Sep 18 2021 [PMID: 34576258] (ICC/IF, Mouse)
• Chen M, Wen X, Gao Y et al. IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury International immunopharmacology Sep 20 2021 [PMID: 34555644] (WB, Mouse)
• Zhao S, Li W, Yu W et al. Exosomal miR-21 from tubular cells contributes to renal fibrosis by activating fibroblasts via targeting PTEN in obstructed kidneys Theranostics Aug 2 2021 [PMID: 34522205] (WB, Mouse)
• Zhu L, Xu F, Kang X et al. The antioxidant N-acetylcysteine promotes immune response and inhibits epithelial-mesenchymal transition to alleviate pulmonary fibrosis in chronic obstructive pulmonary disease by suppressing the VWF/p38 MAPK axis Molecular medicine (Cambridge, Mass.) Sep 3 2021 [PMID: 34479474] (WB, Rat)
• Maity P, Singh K, Krug L Et al. Persistent JunB activation in fibroblasts disrupts stem cell niche interactions enforcing skin aging Cell reports Aug 31 2021 [PMID: 34469740] (ICC/IF, Mouse)
• Zhang S, Wei C, Wang Q Et Al. M2-Polarized Macrophages Mediate Wound Healing by Regulating Connective Tissue Growth Factor via AKT, ERK 1/2, and STAT 3 Signaling Pathways Research Square Jun 22 2021 [PMID: 34398425] (IHC)
• Arias-Garcia M, Rickman R, Sero J The cell-cell adhesion protein JAM3 determines nuclear deformability by regulating microtubule organization biorxiv.org Jan 1 2019 (ICC/IF, Human)
• Montaldo C, Terri M, Riccioni V Et Al. Fibrogenic signals persist in DAA-treated HCV patients after sustained virological response Journal of hepatology Jul 13 2021 [PMID: 34271004]
• Giacalone D, Huang L, Tan S Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology Methods in molecular biology (Clifton, N.J.) Jul 8 2021 [PMID: 34235663]
• Melchor SJ, Hatter JA, Castillo EALT, Saunders CM Cachexia and fibrosis are costs of chronic IL-1R-mediated disease tolerance in T. gondii infection bioRxiv
• Xie T, Kulur V, Liu N et al. Mesenchymal growth hormone receptor deficiency leads to failure of alveolar progenitor cell function and severe pulmonary fibrosis Science advances Jun 1 2021 [PMID: 34108218] (IHC, Mouse)
• Ishii H, Kaneko S, Yanai K et al. MicroRNA Expression Profiling in Diabetic Kidney Disease Translational research : the journal of laboratory and clinical medicine Jun 6 2021 [PMID: 34102327] (IHC, Mouse)
• Song Y, Kim S, Heo J et al. Identification of hepatic fibrosis inhibitors through morphometry analysis of a hepatic multicellular spheroids model Scientific reports May 25 2021 [PMID: 34035369] (WB, Human)
• Ouyang L, Su X, Li W et al. ALKBH1-demethylated DNA N6-methyladenine modification triggers vascular calcification via osteogenic reprogramming in chronic kidney disease The Journal of clinical investigation May 18 2021 [PMID: 34003800] (WB, Human)
• Stevenson NL, Bergen DJM, Lu Y et al. Giantin is required for intracellular N-terminal processing of type I procollagen The Journal of cell biology Jun 7 2021 [PMID: 33944912]
• Wang H, Xu Y, Wang P, et al. Cell-mediated injectable blend hydrogel-BCP ceramic scaffold for in situ condylar osteochondral repair Acta biomaterialia Jan 13 2021 [PMID: 33453407] (IHC-P, Rabbit)
• Shao Q, Feng Y, Wang W et al. Drug carrier for sustained release of withaferin A for pancreatic cancer treatment J Mater Sci
• Ji S, Gumina D, McPeak K et al. Human placental villous stromal extracellular matrix regulates fetoplacental angiogenesis in severe fetal growth restriction Clinical science (London, England : 1979) Apr 27 2021 [PMID: 33904582]
• Suzuki K, Komura M, Obana K et al. A new method of primary engineering of esophagus using orthotopic in-body tissue architecture Journal of Pediatric Surgery Mar 1 2021 [PMID: 33845983]
• Li W, Qi N, Wang S et al. miR-455-5p regulates atrial fibrillation by targeting suppressor of cytokines signaling 3 Journal of physiology and biochemistry Apr 1 2021 [PMID: 33792885]
• Maier JI, Rogg M, HelmstAdter M et al. EPB41L5 controls podocyte extracellular matrix assembly by adhesome-dependent force transmission Cell reports Mar 23 2021 [PMID: 33761352] (ICC/IF, Human)
• Altera A, Tosi GM, Regoli M et al. The extracellular matrix complexity of idiopathic epiretinal membranes and the bilaminar arrangement of the associated internal limiting membrane in the posterior retina Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie Mar 24 2021 [PMID: 33760980]
• Li P, Liu Y, Qin X et al. SIRT1 attenuates renal fibrosis by repressing HIF-2 alpha Cell death discovery Mar 23 2021 [PMID: 33758176] (WB, Mouse)
• Cho HJ, Kim HJ, Lee K et al. Bioengineered Multicellular Liver Microtissues for Modeling Advanced Hepatic Fibrosis Driven Through Non-Alcoholic Fatty Liver Disease Small (Weinheim an der Bergstrasse, Germany) Mar 9 2021 [PMID: 33690979]
• Morimoto C, Takedachi M, Kawasaki K et al. Hypoxia stimulates collagen hydroxylation in gingival fibroblasts and periodontal ligament cells Journal of periodontology Mar 4 2021 [PMID: 33660864]
• Huang L, Zhang Y, Yang J et al. EGR 1 plays a protective role in AMPK inhibitor compound C induced apoptosis through ROS induced ERK activation in skin cancer cellsAnti fibrotic effects and the mechanism of action of miR 29c in silicosis Molecular medicine reports Apr 1 2021 [PMID: 33649785]
• Estermann M, Spiaggia G, Septiadi D et al. Design of Perfused PTFE Vessel-Like Constructs for In Vitro Applications Macromolecular bioscience Feb 24 2021 [PMID: 33624920] (IHC-P, Human)
• Rustenhoven J, Drieu A, Mamuladze T, et al. Functional characterization of the dural sinuses as a neuroimmune interface Cell Jan 18 2021 [PMID: 33508229]
• Kim S, Hong HS Substance-P prevents the cholestatic liver injury by regulating inflammatory responses Peptides Jan 10 2021 [PMID: 33440226] (WB, Rat)
• Rodriguez-Rivera V. Novel Biofabrication Technologies to Recapitulate In Vivo Geometries in Collagen Hydrogels Thesis. 2014 Dec 15 (WB)
• Jian Z, Zhuang T, Qinyu T et al. 3D bioprinting of a biomimetic meniscal scaffold for application in tissue engineering Bioactive materials Jun 1 2021 [PMID: 33313450] (ICC/IF, Rabbit)
• O Shea TM, Wollenberg AL, Kim JH et al. Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions Nature communications Dec 4 2020 [PMID: 33277474] (IHC-Fr, Mouse)
• Mia MM, Cibi DM, Abdul Ghani SAB et al. YAP/TAZ deficiency reprograms macrophage phenotype and improves infarct healing and cardiac function after myocardial infarction PLoS biology Dec 1 2020 [PMID: 33264286] (IHC-Fr, Mouse)
  
  Details:
  Transgenic mouse hearts underwent immunohistochemical analysis.
• Kasetti RB, Patel PD, Maddineni P et al. ATF4 leads to glaucoma by promoting protein synthesis and ER client protein load Cell Stem Cell Oct 27 2020 [PMID: 33154371] (IHC-P)
• Chai, Q, Lu, Z Et al. Lung gene expression signatures suggest pathogenic links and molecular markers for pulmonary tuberculosis, adenocarcinoma and sarcoidosis. Commun Biol Oct 23 2020 [PMID: 33097805] (IHC, WB, Human)
• An M, Kim H, Moon JM et al. Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation Antioxidants (Basel, Switzerland) Sep 24 2020 [PMID: 32987843] (IHC, WB, Mouse)
• Melchor, S J, Hatter, J A Et al. T. gondii infection induces IL-1R dependent chronic cachexia and perivascular fibrosis in the liver and skeletal muscle. Sci Rep Sep 24 2020 [PMID: 32973293] (WB, Human)
• Dawson LA, Schanes PP, Marrero L et al. Proximal digit tip amputation initiates simultaneous blastema and transient fibrosis formation and results in partial regeneration Wound Repair Regen Aug 19 2020 [PMID: 32815252]
• Mietus CJ, Lackner TJ, Karvelis PS et al. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med Aug 8 2020 [PMID: 32784470] (ICC/IF, Bovine, Human)
• Esquibel CR, Wendt KD, Lee HC et al. Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue Front Neurosci Jul 7 2020 [PMID: 32733179]
• Volkova E, Procell L, Kong L et al. Vascular stiffening in aging females with a hypertension induced HIF2A gain of function mutation Bioengineering & Translational Medicine Oct 3 2022 (IHC, Mouse)
• Yang H, An J, Choi I et al. Region-specific astrogliosis: differential vessel formation contributes to different patterns of astrogliosis in the cortex and striatum Mol Brain Jul 22 2020 [PMID: 32698847] (IHC, Mouse)
• Caporarello N, Meridew JA, Aravamudhan A et al. Vascular dysfunction in aged mice contributes to persistent lung fibrosis Aging Cell Jul 21 2020 [PMID: 32691484] (IHC, Human)
• Zhang Y, Jeong H, Mori K et al. Vascular endothelial growth factor from retinal pigment epithelium is essential in choriocapillaris and axial length maintenance PNAS Nexus Aug 24 2022 (IHC, Mouse)
• Wang J, Li G, Hu L et al. Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells Stem Cells Int Jun 27 2020 [PMID: 32676119] (IHC-P, Porcine)
• Li X, Zhao J, Kasinath V et al. Lymph node fibroblastic reticular cells deposit fibrosis-associated collagen following organ transplantation J. Clin. Invest. Jun 29 2020 [PMID: 32597832] (IHC-Fr, Human)
• Gounani Z, Sen Karaman D, Venu AP et al. Coculture of P. aeruginosa and S. aureus on cell derived matrix - An in vitro model of biofilms in infected wounds J. Microbiol. Methods Jun 25 2020 [PMID: 32593628] (ICC/IF, Bacteria)
• Lee BJ, Hegewisch Solloa E, Shannon MJ, Mace EM Generation of cell-derived matrices that support human NK cell migration and differentiation J. Leukoc. Biol. May 11 2020 [PMID: 32392635] (ICC/IF, Human)
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  Details:
  Collagen I alpha 1 antibody used for IHC-P on human kidney allograft protocol biopsies (Fig 1)
• Duarte AS, Cavaleiro E, Pereira C et al. Aeromonas piscicola AH-3 expresses an extracellular collagenase with cytotoxic properties. Lett. Appl. Microbiol. 2014 Dec 02 [PMID: 25443157] (WB)
  
  Details:
  Collagen I alpha 1 antibody used for Far-Western blot analysis on cell-free supernatants (CFSs) from Aeromonas piscicola AH-3 and Aeromonas piscicola AH-3::colAh knockout mutants, with collagen type I as a positive control (Figure 1C).
• Gehmlich K, Dodd MS, William Allwood J et al. Changes in the cardiac metabolome caused by perhexiline treatment in a mouse model of hypertrophic cardiomyopathy. Mol Biosyst. 2014 Dec 01 [PMID: 25437646] (WB, Mouse)
  
  Details:
  Collagen I alpha 1 antibody used in WB for examining the Collagen 1 protein levels in perhexiline- and placebo-treated Mybpc3 KI mice compared to WT, with endogenous protein calsequestrin (CSQ) used as a normalization control. WB data is shown in Figure S4A.
• Fukuda M, Aoki T, Manabe T et al. Exacerbation of Intracranial Aneurysm and Aortic Dissection in Hypertensive Rat Treated With the Prostaglandin F-Receptor Antagonist AS604872. J. Pharmacol. Sci. 2014 Oct 23 [PMID: 25341845] (IHC, Human, Rat)
• Ramani K, Donoyan S, Tomasi Ml et al. Role of Methionine Adenosyltransferase Alpha2 and B Phosphorylation and Stabilization in Human Hepatic Stellate Cell Trans-Differentiation. J. Cell. Physiol. 2014 Oct 07 [PMID: 25294683] (WB, Human)
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  Details:
  Collagen I antibody used for WB on protein extracts from rat's pancreatic stellate cells/ PSCs using PVDF membrane protein transfer and LI-COR -Odyssey detection. Figure 4A - type?I?collagen 150 kDa expression levels in Rat's PSCs treated or not with 48h of 10uM/L erlotinib, sorafenib or sunitinib.
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  Details:
  Collagen I alpha 1 antibody used for IHC-P on 4um sections of dentin extracted from human subject's molars and premolar teeth with no caries and visible anatomic defects (Figure 2).
• Cook J. Toward a Mechanistic Understanding of Hepatic Insulin Action and Resistance. Thesis. 2014 (WB, Mouse)
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Reviews for Collagen I Antibody (NB600-408) (16)

Average Rating: 4.6

(Based on 16 reviews)

We have 16 reviews tested in 5 species: Human, Mouse, Rat, Feline, Other.

reviewed by: Verified Customer IHC-P Rat 05/05/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Rat Lung Tissue
• Species: Rat
• Lot: 47500

reviewed by: Kannan Govindaraj ICC Human 04/23/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: chondrocytes
• Species: Human
• Lot: 44222

reviewed by: Daisy Dai IHC-P Mouse 10/10/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Pancreas
• Species: Mouse
• Lot: 42336

reviewed by: Nin Wan IHC-P Mouse 10/02/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Balb/3T3 mouse embryonic fibroblast cell line
• Species: Mouse
• Lot: 40267

reviewed by: Verified Customer WB Human 05/30/2018

Summary

• Application: Western Blot
• Sample Tested: whole cell extract
• Species: Human
• Lot: 35820

reviewed by: Serina Ng IHC-P Mouse 10/25/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Lot: 38594

reviewed by: MIN KANG WB Human 09/27/2017

Summary

• Application: Western Blot
• Sample Tested: Cell lysate from human trabecular meshwork
• Species: Human
• Lot: 38594

reviewed by: Kazuya Oikawa ICC Feline 06/23/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Adult small intestine
• Species: Feline
• Lot: 38594

Comments

• Comments: 4% PFA fixed feline tissue

reviewed by: Verified Customer IHC-P Rat 02/20/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Bone
• Species: Rat
• Lot: 37789

reviewed by: Sandra Lourenssen IHC-P Rat 07/20/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: rat colon
• Species: Rat
• Lot: 34015

reviewed by: Liyong Zhang WB Other 08/04/2015

Summary

• Application: Western Blot
• Species: Other

reviewed by: Christian Zuppinger IF Human 10/02/2014

Summary

• Application: Immunofluorescence
• Sample Tested: primary human cardiac fibroblasts
• Species: Human
• Lot: 32011

reviewed by: Verified Customer WB Mouse 08/05/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse Flushed Femur and whole cell lysate MC3T3
• Species: Mouse

reviewed by: Wa'el Al Rawashdeh IHC-Fr Human 06/18/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: HaCaT-ras A5-RT3 (SCC) tumor sections
• Species: Human

reviewed by: Verified Customer ICC Human 02/04/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: human dermal fibroblast derived cell sheet
• Species: Human

reviewed by: Daniele Gilkes WB Human 09/04/2012

Summary

• Application: Western Blot
• Sample Tested: Fibroblast Cell Media
• Species: Human

FAQs for Collagen I Antibody (NB600-408). (Showing 1 - 2 of 2 FAQs).

1. Could you please advise if this antibody works for IHC-P application using bone tissues?
   • NB600-408 has been tested and validated for use in the following applications and species: Western blot, ELISA, IP and IHC-Paraffin in human, mouse, rat and bovine. We have not specifically tested bone tissue.
2. Do you follow standard Western procedures with this collagen Ab or can the protein not be denatured?
   • Since we have not posted any special instruction for using this antibody in WB on our web, you could use the standard WB protocol. Although this collagen I a 1 is a huge protein (Uniprot site), the structure is relatively simple; with few modified amino acids and glycosylations. The detection of this protein with SDS-PAGE (denaturing gel) will ensure the molecular size of the protein could be accurately detected and confirmed, even though the glycosylations may alter the true MW. The manufacture of NB600-408 was carried out by the injections of rabbit with the immunogens derived from native collagen Type I fragments derived from human and bovine placenta after the limited pepsin digestions and selective salt precipitations. Therefore this antibody should recognize the native, undenatured collagen 1 alpha 1. If using the undenatured lysates in WB, you should expect to detect a very large protein complexes on your gel/WB, as the collagen I a 1 interacts with itself and/or other components in the extracellular matrices; but our WB protocol should be able to serve the propose.

Bioinformatics

• Gene Symbol: COL1A1
• Entrez:
  • 282187(Bovine)
  • 1277(Human)
  • 29393(Rat)
• Uniprot:
  • P02452()
  • P02452()
  • P02452()
  • P02452()
  • P02452()
  • P02453(Bovine)