Collagen I Antibody

Images

 
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Rat colon tissue stained with Collagen I antibody (red) and Hoechst (blue). IHC-P image submitted by a verified customer review.
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Myofibroblast differentiation requires CD271/NGF activation. Representative alphaSMA (red) and COLLAGEN 1 (red) ...read more
Western Blot: Collagen I Antibody [NB600-408] - Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight 649 ...read more
Western Blot: Collagen I Antibody [NB600-408] - Representative Western blot image for Collagen I, Collagen 2 and GAPDH. N/group = 8. Image collected and cropped by CiteAb from the following publication ...read more
Immunohistochemistry: Collagen I Antibody [NB600-408] - Macro- and micro-architecture of human adipose tissue lobule. Representative immunostaining of the human AT lobule with Collagen I (COL1), CD34, and DAPI. The ...read more
Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Primary human cardiac fibroblast cells were stained with anti-Collagen I antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf ...read more
Western Blot: Collagen I Antibody [NB600-408] - Cell lysate from human trabecular meshwork. Antibody at 1:1.000. WB image submitted by a verified customer review.
Immunohistochemistry: Collagen I Antibody [NB600-408] - Patient-derived CAF and NF CDMs are architecturally and functionally distinct. Representative western blots showing Collagen I (red) and fibronectin (blue) ...read more
Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Staining of human HaCaT-ras A5-RT3 (SCC) frozen tumor sections. Image provided by Dr. Wa'el Al Rawashdeh of RWTH Aachen University.
Western Blot: Collagen I Antibody [NB600-408] - Analysis in porcine burn wound lysate using anti-Collagen I alpha 1 antibody. WB image submitted by a verified customer review.
Western Blot: Collagen I Antibody [NB600-408] - Detection of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Balb/3T3 mouse embryonic fibroblast cell line stained with Collagen I antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Mouse pancreas tissue stained with Collagen I antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Human lung tissue section stained with Collagen I antibody at 1:400.
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - FFPE right lobe of the liver tissue section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat ...read more
Immunohistochemistry: Collagen I Antibody [NB600-408] - Analysis of FFPE human lung. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin at pH 6. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin tissue at pH9. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Imaging of rat calvarial defect bone. IHC-P image submitted by a verified customer review.
Immunohistochemistry: Collagen I Antibody [NB600-408] - Immunohistological staining of EG treated rat kidneys for Collagen. Magnification X40, Scale bar 100 um. Image collected and cropped by CiteAb from the following ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ba, Bv, Rb, Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PAGE
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Collagen I Antibody Summary

Description
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Type-I collagen is the predominant form of collagen in the human body and plays a role in scar tissue formation. Collagen type 1 is also an important component of tendons and found in most connective tissues including cartilage. Collagen 1 is associated with several diseases including: Ehlers Danlos syndrome (arthrochalasia), Ehlers-Danlos syndrome (classical type), Osteogenesis imperfecta type 1-4 and osteoporosis.
Immunogen
Collagen I from human and bovine placenta (Uniprot: P02452)
Localization
Extracellular matrix
Specificity
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Predicted Species
Human (100%), Mouse (92%), Rat (92%), Canine (98%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL1A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:50000
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Microarray
  • SDS-Page
  • Western Blot 1:1000-1:10000
Application Notes
Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, Immunofluorescence, FLOW, and western blotting for highly sensitive qualitative analysis.
Use in SDS-Page reported in scientific literature (PMID: 30914477).
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 15 Reviews rated 4.5
using
NB600-408 in the following applications:

Publications
Read Publications using
NB600-408 in the following applications:

  • 44 publications
  • IF
    1 publication
  • IHC
    37 publications
  • 8 publications
  • 24 publications
  • IP
    1 publication
  • 1 publication
  • 1 publication
  • WB
    59 publications

Reactivity Notes

This antibody reacts with human, bovine, and most mammalian Type I collagens with negligible cross-reactivity with Type II, III, IV, V or VI collagens
Use in Mouse reported in scientific literature (PMID: 33758176).

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Collagen I Antibody

  • Alpha-1 type I collagen
  • alpha1(I) procollagen
  • CAFYD
  • COL1A1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain preproprotein
  • collagen alpha-1(I) chain
  • Collagen I
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • EDSARTH1
  • EDSC
  • OI1
  • OI2
  • OI3
  • OI4
  • pro-alpha-1 collagen type 1
  • type I proalpha 1
  • Type I Procollagen Alpha 1 Chain

Background

Collagen I is one of the most abundant extracellular matrix (ECM) proteins in mammals and is an important structural component in connective tissues, bones, teeth, skin, heart, and lungs (1, 2). Collagen I is commonly used for biomedical purposes including providing an ECM microenvironment for cell attachment, proliferation, and mesenchymal stem cell growth and differentiation to the osteogenic lineage (1, 3, 4). Furthermore, type I Collagen hydrogels are a promising scaffold for engineering tissues and tumors (4). Collagen type I can be extracted from numerous sources including mammals, most commonly bovine and porcine, fish, amphibians, and birds (1, 4). Structurally, Collagen I is a heterotrimer comprised of two alpha-1 chains and one alpha-2 chain that together forms a triple helix (1, 3, 5). Each alpha chain is primarily formed by glycine, proline, and hydroxyproline repeats with the alpha-1 chains having a theoretical molecular weight of 139 kDa and 129 kDa for the alpha-2 chain (3). The dimeric form of Collagen I has a theoretical molecular weight of ~270 kDa while the trimeric form is >400 kDa (3, 5).

A variety of disease pathologies have been associated with mutations in or disruptions in collagen type I. Osteogenesis imperfecta, also known as brittle bone disease, is caused by autosomal dominant mutations in Collagen type I encoding genes and characterized by fragile bones and skeletal deformities (6). Ehlers-Danlos syndromes (EDS) is another disorder that affects soft connective tissue and its various clinical subtypes are associated with different mutations in Collagen I (7).

Alternative names for Collagen I includes alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, and type I procollagen alpha 1 chain.

References

1. Chowdhury, S. R., Mh Busra, M. F., Lokanathan, Y., Ng, M. H., Law, J. X., Cletus, U. C., & Binti Haji Idrus, R. (2018). Collagen Type I: A Versatile Biomaterial. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-13-0947-2_21

2. Kisling, A., Lust, R. M., & Katwa, L. C. (2019). What is the role of peptide fragments of collagen I and IV in health and disease?. Life sciences. https://doi.org/10.1016/j.lfs.2019.04.042

3. Kwon, D., Kang, G. S., Han, D. K., Park, K., Kim, J. H., & Lee, S. H. (2014). Establishment and characterization of human engineered cells stably expressing large extracellular matrix proteins. Archives of pharmacal research. https://doi.org/10.1007/s12272-013-0294-0

4. Antoine, E. E., Vlachos, P. P., & Rylander, M. N. (2014). Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport. Tissue engineering. Part B, Reviews. https://doi.org/10.1089/ten.TEB.2014.0086

5. Leon-Lopez, A., Morales-Penaloza, A., Martinez-Juarez, V. M., Vargas-Torres, A., Zeugolis, D. I., & Aguirre-alvarez, G. (2019). Hydrolyzed Collagen-Sources and Applications. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules24224031

6. Marini, J. C., Forlino, A., Bachinger, H. P., Bishop, N. J., Byers, P. H., Paepe, A., Fassier, F., Fratzl-Zelman, N., Kozloff, K. M., Krakow, D., Montpetit, K., & Semler, O. (2017). Osteogenesis imperfecta. Nature reviews. Disease primers. https://doi.org/10.1038/nrdp.2017.52

7. Callewaert, B., Malfait, F., Loeys, B., & De Paepe, A. (2008). Ehlers-Danlos syndromes and Marfan syndrome. Best practice & research. Clinical rheumatology, 22(1), 165-189. https://doi.org/10.1016/j.berh.2007.12.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen I Antibody (NB600-408)(219)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bacteria, Bovine, Porcine, Rabbit.

We have publications tested in 9 applications: ICC/IF, IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PAGE, WB.


Filter By Application
ICC/IF
(44)
IF
(1)
IHC
(37)
IHC-Fr
(8)
IHC-P
(24)
IP
(1)
MiAr
(1)
PAGE
(1)
WB
(59)
All Applications
Filter By Species
Human
(75)
Mouse
(47)
Rat
(28)
Bacteria
(1)
Bovine
(1)
Porcine
(4)
Rabbit
(4)
All Species
Showing Publications 1 - 10 of 219. Show All 219 Publications.
Publications using NB600-408 Applications Species
Keller CR, Hu Y, Ruud KF Et al. Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells Cancers Nov 22 2021 [PMID: 34831010] (ICC/IF, Human) ICC/IF Human
Kasetti RB, Maddineni P, Kodati B Et al. Astragaloside IV Attenuates Ocular Hypertension in a Mouse Model of TGF beta 2 Induced Primary Open Angle Glaucoma International journal of molecular sciences Nov 19 2021 [PMID: 34830390] (ICC/IF, Human) ICC/IF Human
de Sousa BM, Correia CR, Ferreira JAF Et al. Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants NPJ Regenerative medicine Nov 23 2021 [PMID: 34815414] (WB, Mouse) WB Mouse
Lee J, Ung A, Kim H Et al. Engineering liver microtissues to study the fusion of HepG2 with mesenchymal stem cells and invasive potential of fused cells Biofabrication Nov 30 2021 [PMID: 34740205]
Miura Y, Lam M, Bourke JE, Kanazawa S Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia Life science alliance Jan 1 2022 [PMID: 34728556] (IHC-P, Mouse) IHC-P Mouse
Huang LT, Chou HC, Chen CM. Inhibition of FABP4 attenuates hyperoxia-induced lung injury and fibrosis via inhibiting TGF- beta signaling in neonatal rats Journal of cellular physiology Oct 28 2021 [PMID: 34708870] (Rat) Rat
Maddineni P, Kasetti RB, Kodati B et al. Sodium 4-Phenylbutyrate Reduces Ocular Hypertension by Degrading Extracellular Matrix Deposition via Activation of MMP9 International journal of molecular sciences Sep 18 2021 [PMID: 34576258] (ICC/IF, Mouse) ICC/IF Mouse
Chen M, Wen X, Gao Y et al. IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury International immunopharmacology Sep 20 2021 [PMID: 34555644] (WB, Mouse) WB Mouse
Zhao S, Li W, Yu W et al. Exosomal miR-21 from tubular cells contributes to renal fibrosis by activating fibroblasts via targeting PTEN in obstructed kidneys Theranostics Aug 2 2021 [PMID: 34522205] (WB, Mouse) WB Mouse
Zhu L, Xu F, Kang X et al. The antioxidant N-acetylcysteine promotes immune response and inhibits epithelial-mesenchymal transition to alleviate pulmonary fibrosis in chronic obstructive pulmonary disease by suppressing the VWF/p38 MAPK axis Molecular medicine (Cambridge, Mass.) Sep 3 2021 [PMID: 34479474] (WB, Rat) WB Rat
Show All 219 Publications.

Reviews for Collagen I Antibody (NB600-408) (15) 4.515

Average Rating: 4.5
(Based on 15 reviews)
We have 15 reviews tested in 5 species: Human, Mouse, Rat, Feline, Other.

Reviews using NB600-408:
Filter by Applications
ICC
(3)
IHC-P
(5)
WB
(5)
IF
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(7)
Mouse
(4)
Rat
(2)
Feline
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Collagen I NB600-408
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5
reviewed by:
Verified Customer
ICC Human 04/23/2021
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Summary

ApplicationImmunocytochemistry
Sample Tested chondrocytes
SpeciesHuman
Lot44222
Immunohistochemistry-Paraffin Collagen I NB600-408
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5
reviewed by:
Verified Customer
IHC-P Mouse 10/10/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
Lot42336
Immunohistochemistry-Paraffin Collagen I NB600-408
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4
reviewed by:
Verified Customer
IHC-P Mouse 10/02/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBalb/3T3 mouse embryonic fibroblast cell line
SpeciesMouse
Lot40267
  5
reviewed by:
Wangsheng Wang
WB Human 05/30/2018
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Summary

ApplicationWestern Blot
Sample Testedwhole cell extract
SpeciesHuman
Lot35820
Immunohistochemistry-Paraffin Collagen I NB600-408
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3
reviewed by:
Verified Customer
IHC-P Mouse 10/25/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedIHC on parrafin sections (mouse)
SpeciesMouse
Lot38594
Western Blot Collagen I NB600-408
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5
reviewed by:
Verified Customer
WB Human 09/27/2017
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Summary

ApplicationWestern Blot
Sample TestedCell lysate from human trabecular meshwork
SpeciesHuman
Lot38594
Immunocytochemistry Collagen I NB600-408
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4
reviewed by:
Verified Customer
ICC Feline 06/23/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedAdult small intestine
SpeciesFeline
Lot38594

Comments

Comments4% PFA fixed feline tissue
Immunohistochemistry-Paraffin Collagen I NB600-408
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5
reviewed by:
Jakob Townsend
IHC-P Rat 02/20/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBone
SpeciesRat
Lot37789
Immunohistochemistry-Paraffin Collagen I NB600-408
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5
reviewed by:
Verified Customer
IHC-P Rat 07/20/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedrat colon
SpeciesRat
Lot34015
Western Blot Collagen I NB600-408
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4
reviewed by:
Verified Customer
WB Other 08/04/2015
View

Summary

ApplicationWestern Blot
SpeciesOther
FileView PDF
Immunofluorescence Collagen I NB600-408
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4
reviewed by:
Verified Customer
IF Human 10/02/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedprimary human cardiac fibroblasts
SpeciesHuman
Lot32011

Blocking

Blocking Detailsblocking step for 1 h with 2% bovine serum albumin in PBS at room temperature

Primary Anitbody

Dilution Ratioprimary antibody diluted 1:00 in BSA/PBS, incubation at 4C overnight

Secondary Antibody

Secondary Descriptiongoat anti rabbit Alexa488
Secondary Manufacturer Cat#A-21222
Secondary Concentration1:200

Details

Detection Notesconfocal microscopy, Zeiss LSM710
Fixation Details15 min fixation with 3% para-formaldehyde in PBS at room temp, permeabilization with 10% Triton-X 100 in PBS for 15 min
Wash Descriptionwashing steps with PBS, usually 3 times for 5 minutes
Western Blot Collagen I NB600-408
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4
reviewed by:
Elizabeth Rendina-Ruedy
WB Mouse 08/05/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse Flushed Femur and whole cell lysate MC3T3
SpeciesMouse
CommentsAntibody was easily used to detect 4 forms of type 1 collagen in the mouse femur. Sometimes this would appear as a very large molecular weight smear, but considering the amount of collagen in bone and the various forms, we believe this was acceptable. Due to the large molecular weight we were using 4-20% gradient gels, which again helped us visualize all 4 forms.

Blocking

Blocking Details5% NFDM in 0.05% TTBS for 1 hour at RT

Primary Anitbody

Dilution Ratio1:1000 in 5% NFDM in 0.05% TTBS overnight at 4 degrees C

Secondary Antibody

Secondary DescriptionGoast anti-rabbit, HRP conjugate
Secondary Concentration1:20,000

Details

Detection NotesSuper Signal Pico, Pierce

Comments

CommentsAntibody was easily used to detect 4 forms of type 1 collagen in the mouse femur. Sometimes this would appear as a very large molecular weight smear, but considering the amount of collagen in bone and the various forms, we believe this was acceptable. Due to the large molecular weight we were using 4-20% gradient gels, which again helped us visualize all 4 forms.
Immunohistochemistry-Frozen Collagen I NB600-408
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5
reviewed by:
Verified Customer
IHC-Fr Human 06/18/2013
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedHaCaT-ras A5-RT3 (SCC) tumor sections
SpeciesHuman
CommentsNB600-408 has worked perfectly for staining ColI in frozen tumor samples, always reproducible results

Secondary Antibody

Secondary Descriptionanti-rabbit Cy-2 antibody (Dianova), host species: donkey, dilution 1:100 in 12% BSA

Details

Detection NotesIF Microscope
Fixation DetailsFixation:5 min 80% Methanol @ RT, 2 min Aceton @ -20C.
Wash Description1x dH2O, 3x 10 min PBS, 1x dH2O

Comments

CommentsNB600-408 has worked perfectly for staining ColI in frozen tumor samples, always reproducible results
Immunocytochemistry Collagen I NB600-408
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5
reviewed by:
Nathan Weidenhamer
ICC Human 02/04/2013
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Summary

ApplicationImmunocytochemistry
Sample Testedhuman dermal fibroblast derived cell sheet
SpeciesHuman

Blocking

Blocking Details5% normal donkey serum, 1 hr at room temp.

Primary Anitbody

Dilution Ratio5 ug/ml in 5% normal donkey serum, overnight at 4 degrees C

Secondary Antibody

Secondary Descriptiondonkey anti-rabbit Cy3
Secondary Manufacturer Cat#Jackson ImmunoResearch, 711-165-152
Secondary Concentration4 ug/ml in PBS

Details

Fixation Details4% PFA, 1 hr at room temp.
Wash Description3 x 10 min, PBS
Western Blot Collagen I NB600-408
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5
reviewed by:
Verified Customer
WB Human 09/04/2012
View

Summary

ApplicationWestern Blot
Sample TestedFibroblast Cell Media
SpeciesHuman
CommentsThe antibody works well and seems to detect both N and C cleaved collagen 1 fragments (see blot) as well as the mature collagen form. Western blot was done under denaturing conditions so not restricted to non-denaturing gels. Antibody could be diluted to higher than 1:1000.

Blocking

Blocking Details5% Milk in PBS-T, 20min, RT

Primary Anitbody

Dilution Ratio1:1000 in 5% Milk in PBS-T, O/N, 4C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP
Secondary Concentration1:5000

Details

Detection NotesECL Prime, 10 seconds

Comments

CommentsThe antibody works well and seems to detect both N and C cleaved collagen 1 fragments (see blot) as well as the mature collagen form. Western blot was done under denaturing conditions so not restricted to non-denaturing gels. Antibody could be diluted to higher than 1:1000.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen I Antibody (NB600-408). (Showing 1 - 2 of 2 FAQs).

  1. Could you please advise if this antibody works for IHC-P application using bone tissues?
    • NB600-408 has been tested and validated for use in the following applications and species: Western blot, ELISA, IP and IHC-Paraffin in human, mouse, rat and bovine. We have not specifically tested bone tissue.
  2. Do you follow standard Western procedures with this collagen Ab or can the protein not be denatured?
    • Since we have not posted any special instruction for using this antibody in WB on our web, you could use the standard WB protocol. Although this collagen I a 1 is a huge protein (Uniprot site), the structure is relatively simple; with few modified amino acids and glycosylations. The detection of this protein with SDS-PAGE (denaturing gel) will ensure the molecular size of the protein could be accurately detected and confirmed, even though the glycosylations may alter the true MW. The manufacture of NB600-408 was carried out by the injections of rabbit with the immunogens derived from native collagen Type I fragments derived from human and bovine placenta after the limited pepsin digestions and selective salt precipitations. Therefore this antibody should recognize the native, undenatured collagen 1 alpha 1. If using the undenatured lysates in WB, you should expect to detect a very large protein complexes on your gel/WB, as the collagen I a 1 interacts with itself and/or other components in the extracellular matrices; but our WB protocol should be able to serve the propose.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-408AF350
Alexa Fluor 405 NB600-408AF405
Alexa Fluor 488 NB600-408AF488
Alexa Fluor 532 NB600-408AF532
Alexa Fluor 594 NB600-408AF594
Alexa Fluor 647 NB600-408AF647
Alexa Fluor 700 NB600-408AF700
Alexa Fluor 750 NB600-408AF750
Biotin NB600-408B
DyLight 350 NB600-408UV
DyLight 405 NB600-408V
DyLight 488 NB600-408G
DyLight 550 NB600-408R
DyLight 594 NB600-408DL594
DyLight 650 NB600-408C
DyLight 680 NB600-408FR
DyLight 755 NB600-408IR
FITC NB600-408F
HRP NB600-408H
Janelia Fluor 549 NB600-408JF549
Janelia Fluor 646 NB600-408JF646

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Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

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04/23/2021
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Species: Human

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Bioinformatics

Gene Symbol COL1A1
Entrez
Uniprot