Collagen I Antibody

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Rat colon tissue stained with Collagen I antibody (red) and Hoechst (blue). IHC-P image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Myofibroblast differentiation requires CD271/NGF activation. Representative alphaSMA (red) and COLLAGEN 1 (red) immunostaining with DAPI (blue) in non-treated (control), TGFbeta-treated cells with or without gamma-secretase inhibitor. Scale bars: 50 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY licence.

Western Blot: Collagen I Antibody [NB600-408] - Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.

Western Blot: Collagen I Antibody [NB600-408] - Representative Western blot image for Collagen I, Collagen 2 and GAPDH. N/group = 8. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.12367) licensed under a CC-BY licence.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Macro- and micro-architecture of human adipose tissue lobule. Representative immunostaining of the human AT lobule with Collagen I (COL1), CD34, and DAPI. The position of the septa is underlined, scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Primary human cardiac fibroblast cells were stained with anti-Collagen I antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf serum. ICC/IF image submitted by a verified customer review.

Western Blot: Collagen I Antibody [NB600-408] - Cell lysate from human trabecular meshwork. Antibody at 1:1.000. WB image submitted by a verified customer review.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Patient-derived CAF and NF CDMs are architecturally and functionally distinct. Representative western blots showing Collagen I (red) and fibronectin (blue) staining of patient #1 and #3 NF and CAF CDM. Scale bar, 20 um. (f) Representative SEM images of TIFF, Patient #1 NF and CAF CDM. Scale bar, 5 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms12237) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Staining of human HaCaT-ras A5-RT3 (SCC) frozen tumor sections. Image provided by Dr. Wa'el Al Rawashdeh of RWTH Aachen University.

Western Blot: Collagen I Antibody [NB600-408] - Analysis in porcine burn wound lysate using anti-Collagen I alpha 1 antibody. WB image submitted by a verified customer review.

Western Blot: Collagen I Antibody [NB600-408] - Detection of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2-2 ug/10 mL in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% non-fat milk. Detection occurred by incubation with a HRP conjugated secondary antibody at 1 ug/10 mL. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Balb/3T3 mouse embryonic fibroblast cell line stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Mouse pancreas tissue stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Human lung tissue section stained with Collagen I antibody at 1:400.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - FFPE right lobe of the liver tissue section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Analysis of FFPE human lung. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin at pH 6. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin tissue at pH9. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Imaging of rat calvarial defect bone. IHC-P image submitted by a verified customer review.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Immunohistological staining of EG treated rat kidneys for Collagen. Magnification X40, Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0185009), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Po, Ba, Bv, Fe, Rb
• Applications: WB, ELISA, FLISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Collagen I Antibody Summary

• Description: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Type-I collagen is the predominant form of collagen in the human body and plays a role in scar tissue formation. Collagen type 1 is also an important component of tendons and found in most connective tissues including cartilage. Collagen 1 is associated with several diseases including: Ehlers Danlos syndrome (arthrochalasia), Ehlers-Danlos syndrome (classical type), Osteogenesis imperfecta type 1-4 and osteoporosis.
• Immunogen: This Collagen I antibody was raised against Collagen I from human and bovine placenta. Uniprot ID P02452
• Localization: Extracellular matrix
• Specificity: This Collagen I antibody has typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • ELISA 1:5000 - 1:50000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Microarray
• Theoretical MW: 139 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected to react with most mammalian Type I collagens. Porcine reactivity reported in scientific literature (PMID: 21688145). Rabbit reactivity reported in scientific literature (PMID: 27663536). Use in P. aeruginosa and S. aureus reported in secitific publication PMID: 32593628

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Collagen type I consists of alpha-1 (139 kDa) and alpha-2 chains (129kDa). Since collagen type I is a triple helix consisting of one alpha-2 chain and two alpha-1 chains, one can expect bands of the dimeric (~270 kDa) and the trimeric form (~400 kDa). Remember that those chains are cross-linked and can't be broken by typical sample denaturation for SDS-PAGE.

Alternate Names for Collagen I Antibody

• Alpha-1 type I collagen
• alpha1(I) procollagen
• CAFYD
• COL1A1
• Collagen 1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain preproprotein
• collagen alpha-1(I) chain
• Collagen I
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• EDSARTH1
• EDSC
• OI1
• OI2
• OI3
• OI4
• pro-alpha-1 collagen type 1
• type I proalpha 1
• Type I Procollagen Alpha 1 Chain

Background

Collagen I is one of the most abundant extracellular matrix (ECM) proteins in mammals and is an important structural component in connective tissues, bones, teeth, skin, heart, and lungs (1, 2). Collagen I is commonly used for biomedical purposes including providing an ECM microenvironment for cell attachment, proliferation, and mesenchymal stem cell growth and differentiation to the osteogenic lineage (1, 3, 4). Furthermore, type I Collagen hydrogels are a promising scaffold for engineering tissues and tumors (4). Collagen type I can be extracted from numerous sources including mammals, most commonly bovine and porcine, fish, amphibians, and birds (1, 4). Structurally, Collagen I is a heterotrimer comprised of two alpha-1 chains and one alpha-2 chain that together forms a triple helix (1, 3, 5). Each alpha chain is primarily formed by glycine, proline, and hydroxyproline repeats with the alpha-1 chains having a theoretical molecular weight of 139 kDa and 129 kDa for the alpha-2 chain (3). The dimeric form of Collagen I has a theoretical molecular weight of ~270 kDa while the trimeric form is >400 kDa (3, 5).

A variety of disease pathologies have been associated with mutations in or disruptions in collagen type I. Osteogenesis imperfecta, also known as brittle bone disease, is caused by autosomal dominant mutations in Collagen type I encoding genes and characterized by fragile bones and skeletal deformities (6). Ehlers-Danlos syndromes (EDS) is another disorder that affects soft connective tissue and its various clinical subtypes are associated with different mutations in Collagen I (7).

Alternative names for Collagen I includes alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, and type I procollagen alpha 1 chain.

References

1. Chowdhury, S. R., Mh Busra, M. F., Lokanathan, Y., Ng, M. H., Law, J. X., Cletus, U. C., & Binti Haji Idrus, R. (2018). Collagen Type I: A Versatile Biomaterial. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-13-0947-2_21

2. Kisling, A., Lust, R. M., & Katwa, L. C. (2019). What is the role of peptide fragments of collagen I and IV in health and disease?. Life sciences. https://doi.org/10.1016/j.lfs.2019.04.042

3. Kwon, D., Kang, G. S., Han, D. K., Park, K., Kim, J. H., & Lee, S. H. (2014). Establishment and characterization of human engineered cells stably expressing large extracellular matrix proteins. Archives of pharmacal research. https://doi.org/10.1007/s12272-013-0294-0

4. Antoine, E. E., Vlachos, P. P., & Rylander, M. N. (2014). Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport. Tissue engineering. Part B, Reviews. https://doi.org/10.1089/ten.TEB.2014.0086

5. Leon-Lopez, A., Morales-Penaloza, A., Martinez-Juarez, V. M., Vargas-Torres, A., Zeugolis, D. I., & Aguirre-alvarez, G. (2019). Hydrolyzed Collagen-Sources and Applications. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules24224031

6. Marini, J. C., Forlino, A., Bachinger, H. P., Bishop, N. J., Byers, P. H., Paepe, A., Fassier, F., Fratzl-Zelman, N., Kozloff, K. M., Krakow, D., Montpetit, K., & Semler, O. (2017). Osteogenesis imperfecta. Nature reviews. Disease primers. https://doi.org/10.1038/nrdp.2017.52

7. Callewaert, B., Malfait, F., Loeys, B., & De Paepe, A. (2008). Ehlers-Danlos syndromes and Marfan syndrome. Best practice & research. Clinical rheumatology, 22(1), 165-189. https://doi.org/10.1016/j.berh.2007.12.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen I Antibody (NB600-408)(182)

Publications using NB600-408

• Shao Q, Feng Y, Wang W et al. Drug carrier for sustained release of withaferin A for pancreatic cancer treatment J Mater Sci
• Mietus CJ, Lackner TJ, Karvelis PS et al. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med Aug 8 2020 [PMID: 32784470] (ICC/IF, Bovine, Human)
• O'Shea TM, Wollenberg AL, Kim JH et al. Foreign body responses in central nervous system mimic natural wound responses and alter biomaterial functions bioRxiv 2019 Oct 08 (IHC, Mouse)
• Vodyanoy V, Pustovyy O, Globa L et al. Hemmule: A Novel Structure with the Properties of the Stem Cell Niche Preprints Jun 24 2019 (IHC, Rat)
• Ren J, Chakrabarti S, Wu J. Phosvitin and its hydrolysate promote differentiation and inhibit TNF-alpha induced inflammation in MC3T3-E1 cells via ERK and AKT pathways Journal of Functional Foods Feb 1 2019 (ICC/IF, Mouse)
• Kasetti RB, Patel PD, Maddineni P et al. ATF4 leads to glaucoma by promoting protein synthesis and ER client protein load Cell Stem Cell Oct 27 2020 [PMID: 33154371] (IHC-P)
• An M, Kim H, Moon JM et al. Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation Antioxidants (Basel, Switzerland) Sep 24 2020 [PMID: 32987843] (IHC, WB, Mouse)
• Dawson LA, Schanes PP, Marrero L et al. Proximal digit tip amputation initiates simultaneous blastema and transient fibrosis formation and results in partial regeneration Wound Repair Regen Aug 19 2020 [PMID: 32815252]
• Esquibel CR, Wendt KD, Lee HC et al. Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue Front Neurosci Jul 7 2020 [PMID: 32733179]
• Yang H, An J, Choi I et al. Region-specific astrogliosis: differential vessel formation contributes to different patterns of astrogliosis in the cortex and striatum Mol Brain Jul 22 2020 [PMID: 32698847] (IHC, Mouse)
• Caporarello N, Meridew JA, Aravamudhan A et al. Vascular dysfunction in aged mice contributes to persistent lung fibrosis Aging Cell Jul 21 2020 [PMID: 32691484] (IHC, Human)
• Wang J, Li G, Hu L et al. Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells Stem Cells Int Jun 27 2020 [PMID: 32676119] (IHC-P, Porcine)
• Li X, Zhao J, Kasinath V et al. Lymph node fibroblastic reticular cells deposit fibrosis-associated collagen following organ transplantation J. Clin. Invest. Jun 29 2020 [PMID: 32597832] (IHC-Fr, Human)
• Gounani Z, Sen Karaman D, Venu AP et al. Coculture of P. aeruginosa and S. aureus on cell derived matrix - An in vitro model of biofilms in infected wounds J. Microbiol. Methods Jun 25 2020 [PMID: 32593628] (ICC/IF, Bacteria)
• Lee BJ, Hegewisch Solloa E, Shannon MJ, Mace EM Generation of cell-derived matrices that support human NK cell migration and differentiation J. Leukoc. Biol. May 11 2020 [PMID: 32392635] (ICC/IF, Human)
• Tran-Nguyen TK, Xue J, Feghali-Bostwick C et al. CD70 Activation Decreases Pulmonary Fibroblast Production of Extracellular Matrix Proteins Am. J. Respir. Cell Mol. Biol. Apr 22 2020 [PMID: 32320626]
• Kang Y, Roh MR, Rajadurai S et al. Hypoxia and HIF-1 alpha Regulate Collagen Production in Keloids J. Invest. Dermatol. Apr 18 2020 [PMID: 32315657]
• Kim J, Park M, Paek J et al. Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman's Syndrome Front. Physiol. Feb 14 2020 [PMID: 32116803] (IHC-Fr, Mouse)
• Purdy MP, Ducharme M, Haak AJ et al. YAP/TAZ are Activated by Mechanical and Hormonal Stimuli in Myometrium and Exhibit Increased Baseline Activation in Uterine Fibroids Reprod Sci Feb 13 2020 [PMID: 32056132]
• Khamar P, Nishtala K, Shetty R et al. Early biological responses in ocular tissue after SMILE and LASIK surgery Exp. Eye Res. Jan 27 2020 [PMID: 32001250] (IHC-P, Human)
• Canes L, Marti-Pamies I, Ballester-Servera C et al. Neuron-derived orphan receptor-1 modulates cardiac gene expression and exacerbates angiotensin II-induced cardiac hypertrophy Clin. Sci. Jan 27 2020 [PMID: 31985010]
• Minna E, Brich S, Todoerti K et al. Cancer Associated Fibroblasts and Senescent Thyroid Cells in the Invasive Front of Thyroid Carcinoma Cancers (Basel) Jan 1 2020 [PMID: 31906302] (IHC-P, IHC, Human)
• Regoli M, Tosi GM, Neri G The Peculiar Pattern of Type IV Collagen Deposition in Epiretinal Membranes J. Histochem. Cytochem. Dec 20 2019 [PMID: 31858878] (ICC/IF, Human)
• Liu Z, Qian M, Tang X, et al. Targeted regulation of fibroblast state by CRISPR-mediated CEBPA expression Respir. Res. Dec 11 2019 [PMID: 31829168] (ICC/IF, Human)
• Aljohani H, Senbanjo LT, Chellaiah MA Methylsulfonylmethane increases osteogenesis and regulates the mineralization of the matrix by transglutaminase 2 in SHED cells PLoS ONE Dec 5 2019 [PMID: 31805069] (WB, Human)
• Choi JH, Kim SM, Lee GH et al. Platyconic Acid A, Platycodi Radix-Derived Saponin, Suppresses TGF-1-induced Activation of Hepatic Stellate Cells via Blocking SMAD and Activating the PPAR Signaling Pathway Cells. Nov 29 2019 [PMID: 31795488] (WB, Rat)
• Fu C, Lu Y, Williams MA, et al. Emergency myelopoiesis contributes to immune cell exhaustion and pulmonary vascular remodeling Br. J. Pharmacol. Dec 3 2019 [PMID: 31793661] (WB, Mouse)
• Chen PY, Qin L, Li G et al. Endothelial TGF-beta signalling drives vascular inflammation and atherosclerosis Nat Metab Sep 1 2019 [PMID: 31572976] (IHC, Mouse)
• Benvenuto M, Mattera R, Miele MT et al. Effects of a natural multi-component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts Exp Ther Med [PMID: 31572512]
• Balasubramanian M, Hobson E, Skae M et al. Developing pathways to clarify pathogenicity of unclassified variants in Osteogenesis Imperfecta genetic analysis Mol Genet Genomic Med Sep 30 2019 [PMID: 31568717] (WB, ICC/IF, Human)
• Elrashidy RA, Liu G Long-term diabetes causes molecular alterations related to fibrosis and apoptosis in rat urinary bladder Exp. Mol. Pathol. Aug 31 2019 [PMID: 31479659]
• Vidoni C, Ferraresi A, Secomandi E et al. Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells Cell Commun. Signal Aug 19 2019 [PMID: 31426798] (WB, ICC/IF, Human)
• Sato K, Yoshizawa H, Seki T et al. Chemerin-9, a potent agonist of chemerin receptor (ChemR23), prevents atherogenesis Clin. Sci. Aug 30 2019 [PMID: 31399499] (WB, Human)
• Melchor SJ, Hatter JA, Castillo EALT, Saunders CM Cachexia and fibrosis are costs of chronic IL-1R-mediated disease tolerance in T. gondii infection bioRxiv
• Suo H, Li L, Zhang C et al. Glucosamine-grafted methacrylated gelatin hydrogels as potential biomaterials for cartilage repair J. Biomed. Mater. Res. Part B Appl. Biomater. Aug 1 2019 [PMID: 31369700]
• Guo Xiangjiang, Fereydooni Arash, Isaji Toshihiko et al. Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency. Scientific Reports 2019 Jul 30 [PMID: 31363142] (IHC-P, Mouse)
• Xue YZ, Li ZJ, Liu WT et al. Down-regulation of lncRNA MALAT1 alleviates vascular lesion and vascular remodeling of rats with hypertension Aging (Albany NY) Jul 25 2019 [PMID: 31343412] (WB, Rat)
• van de Vlekkert D, Demmers J, Nguyen XX et al. Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis Sci Adv Jul 1 2019 [PMID: 31328155] (ICC/IF, IHC, WB, Mouse)
• Obata T, Tsuchiya T, Akita S et al. Utilization of natural detergent potassium laurate for decellularization in lung bioengineering Tissue Eng Part C Methods Jul 18 2019 [PMID: 31317819] (MiAr, Human)
• Xu L, Sharkey D, Cantley LG Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury J. Am. Soc. Nephrol. Jul 17 2019 [PMID: 31315923] (WB, Mouse)
• Goldstein RL, Tsui JM, Runyan G et al. Photochemical Tissue Passivation Prevents Contracture of Full Thickness Wounds in Mice Lasers Surg Med Jul 6 2019 [PMID: 31278757] (IHC, Mouse)
• Hashimoto Y, Tsuchiya T, Doi R et al. Alteration of the extracellular matrix and alpha-gal antigens in the rat lung scaffold reseeded using human vascular and adipogenic stromal cells J Tissue Eng Regen Med Jul 2 2019 [PMID: 31267683] (IHC, Rat, Human)
• Esteve D, Boulet N, Belles C et al. Lobular architecture of human adipose tissue defines the niche and fate of progenitor cells Nat Commun 2019 Jun 11 [PMID: 31186409] (IF, Human)
• Mehta N, Gava AL, Zhang D et al. Follistatin Protects Against Glomerular Mesangial Cell Apoptosis and Oxidative Stress to Ameliorate Chronic Kidney Disease Antioxid. Redox Signal. Jul 3 2019 [PMID: 31184201] (WB)
• Li Y, Wang W, Chao Y et al. CTRP13 attenuates vascular calcification by regulating Runx2 FASEB J. May 30 2019 [PMID: 31145871]
• Chiavari M, Ciotti GMP, Navarra P, Lisi L CBX5/G9a/H3K9me-mediated gene repression is essential to fibroblast activation during lung fibrosis JCI Insight May 16 2019 [PMID: 31095524] (Mouse, Human)
• Ansari M, Ahmad A, Mishra R et al. Glucocorticoid Receptor Transactivation Is Required for Glucocorticoid-Induced Ocular Hypertension and Glaucoma Invest. Ophthalmol. Vis. Sci. May 1 2019 [PMID: 31050723] (IHC-P, ICC/IF, Mouse)
• Legrand JMD, Chan AL, La HM et al. Nascent Lung Organoids Reveal Epithelium- and BMP-Mediated Suppression of Fibroblast Activation Am. J. Respir. Cell Mol. Biol. May 3 2019 [PMID: 31050552] (IHC, Human)
• Uehara, M;Li, X;Sheikhi, A;Zandi, N;Walker, B;Saleh, B;Banouni, N;Jiang, L;Ordikhani, F;Dai, L;Yonar, M;Vohra, I;Kasinath, V;Orgill, DP;Khademhosseini, A;Annabi, N;Abdi, R; Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival Sci Rep Apr 25 2019 [PMID: 31024011] (ICC/IF, Mouse)
• Van Krieken R, Mehta N et al. Cell surface expression of 78-kDa glucose-regulated protein (GRP78) mediates diabetic nephropathy. J Biol Chem Oct 5 2019 [PMID: 30914477] (PAGE, Rat)
• MacDonald JA, Takai Y, Ishihara O et al. Extracellular matrix signaling activates differentiation of adult ovary derived oogonial stem cells in a species specific manner Fertil. Steril. Apr 1 2019 [PMID: 30871765] (IHC-P, Mouse)
• Wang C, Xu W, An J et al. Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2 Nat Commun Mar 13 2019 [PMID: 30867423] (WB, Rat)
• Lee M, Song Y, Choi I et al. Expression of HYOU1 via reciprocal crosstalk between NSCLC cells and HUVECs control cancer progression and chemoresistance in multicellular tumor spheroids Research Square Aug 20 2020 [PMID: 30709379]
• McCaughey J, Stevenson N, Cross S, Stephens D. ER-to-Golgi trafficking of procollagen in the absence of large carriers. J Cell Biol. [PMID: 30587510] (ICC/IF, Human)
• Chen HJ, Shuler ML. Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization Methods Mol. Biol. Dec 14 2019 [PMID: 30542993] (IHC, Human)
• Peretti GM, Polito U, Di Giancamillo M et al. Swine Meniscus: Are Femoral-Tibial Surfaces Properly Tuned To Bear The Forces Exerted On The Tissue. Tissue Eng Part A. Nov 6 2018 [PMID: 30398398] (IHC-P, Porcine)
• Tian HP, Sun YH, He L et al. Single-Stranded DNA-Binding Protein 1 Abrogates Cardiac Fibroblast Proliferation and Collagen Expression Induced by Angiotensin II. Int Heart J. Oct 25 2018 [PMID: 30369577] (WB, Mouse)
• Chakraborty S, Jiang C, Gau D et al. Profilin-1 deficiency leads to SMAD3 upregulation and impaired 3D outgrowth of breast cancer cells. Br. J. Cancer. Oct 15 2018 [PMID: 30318519] (Human)
• Jerrell RJ, Leih MJ, Parekh A. The altered mechanical phenotype of fetal fibroblasts hinders myofibroblast differentiation Wound Repair Regen Oct 13 2018 [PMID: 30315715] (ICC/IF, Human)
• Chellaiah MA, Majumdar S, Aljohani H. Peptidomimetic inhibitors of L-plastin reduce the resorptive activity of osteoclast but not the bone forming activity of osteoblasts in vitro. PLoS ONE. Sep 24 2018 [PMID: 30248139] (WB, Rat)
• Ren J, Chakrabarti S, Wu J. Phosvitin and its hydrolysate promote differentiation and inhibit TNF-alpha induced inflammation in MC3T3-E1 cells via ERK and AKT pathways Journal of Functional Foods Feb 1 2019 (ICC/IF, Human)
• Weber G, Purkayastha B, Ren L et al. Hypertension exaggerates renovascular resistance via miR-122-associated stress response in aging Journal of Hypertension Jul 1 2018 [PMID: 30256768]
• Janke HP, Guvener N, Dou W et al. Labeling of Collagen Type I Templates with a Naturally Derived Contrast Agent for Noninvasive MR Imaging in Soft Tissue Engineering Adv Healthc Mater Jul 29 2018 [PMID: 30058274] (IHC-Fr, Mouse)
• Bricambert J, Alves-Guerra MC, Esteves P et al. The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity Nat Commun May 29 2018 [PMID: 29844386] (IHC-P, WB, Mouse)
• Yuan CL, Liang R, Liu ZH et al. Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-B/epithelial-mesenchymal transition signaling pathway Exp Ther Med Jun 1 2018 [PMID: 29805551] (WB, IHC, Human)
• Nguyen DT, O'Hara M, Graneli C et al. Humanizing Miniature Hearts through 4-Flow Cannulation Perfusion Decellularization and Recellularization Sci Rep May 10 2018 [PMID: 29748585] (IHC-P, Rat)
• Pan J, Zhan C, Yuan T et al. Effects and molecular mechanisms of intrauterine infection/inflammation on lung development Respir. Res. May 10 2018 [PMID: 29747649] (IHC, Rat)
• Kasetti RB, Maddineni P, Patel P et al. Transforming growth factor B2 (TGFB2) signaling plays a key role in glucocorticoid-induced ocular hypertension J. Biol. Chem. May 9 2018 [PMID: 29743238] (Mouse)
• Kwon TR, Kim JH, Seok J et al. Fractional CO2 laser treatment for vaginal laxity: A preclinical study Lasers Surg Med May 7 2018 [PMID: 29733104] (IHC, Human)
• Sato K, Yamashita T, Shirai R et al. Adropin Contributes to Anti-Atherosclerosis by Suppressing Monocyte-Endothelial Cell Adhesion and Smooth Muscle Cell Proliferation Int J Mol Sci. [PMID: 29701665] (WB, Human)
• Oh RS, Haak AJ, Smith KMJ et al. RNAi screening identifies a mechanosensitive ROCK-JAK2-STAT3 network central to myofibroblast activation. J. Cell. Sci. Apr 20 2018 [PMID: 29678906] (WB, Human)
• Pietrovito L, Leo A, Gori V et al. Bone marrow-derived mesenchymal stem cells promote invasiveness and transendothelial migration of osteosarcoma cells via a mesenchymal to amoeboid transition. Mol Oncol. Mar 8 2018 [PMID: 29517849] (WB)
• Sicard D, Haak AJ, Choi KM, Craig AR. Aging and Anatomical Variations in Lung Tissue Stiffness. Am. J. Physiol. Lung Cell Mol. Physiol. 2018 Feb 22 [PMID: 29469613] (Human)
• Na J, Bak DH, Im SI, Choi H. Anti-apoptotic effects of glycosaminoglycans via inhibition of ERK/AP-1 signaling in TNF-a-stimulated human dermal fibroblasts. Int. J. Mol. Med. 2018 Feb 12 [PMID: 29436595] (Human)
• Townsend J, Zabel T, Feng Y, Wang J. Effects of tissue processing on bioactivity of cartilage matrix-based hydrogels encapsulating osteoconductive particles. Biomed. Mater. 2018 Feb 07 [PMID: 29411714] (IHC, Rat)
• Rogerson C, Gissen P. VPS33B and VIPAR are essential for epidermal lamellar body biogenesis and function. Biochim. Biophys. Acta. 2018 Jan 30 [PMID: 29409756] (IHC, Mouse)
• Wu X, Zhi F, Lun W, Deng Q. Baicalin inhibits PDGF-BB-induced hepatic stellate cell proliferation, apoptosis, invasion, migration and activation via the miR-3595/ACSL4 axis. Int. J. Mol. Med. 2018 Apr 01 [PMID: 29393361] (WB, Rat)
• Balasubramanian M, Padidela R, Pollitt RC et al. P4HB recurrent missense mutation causing Cole-Carpenter syndrome. J Med Genet. [PMID: 29263160] (Human)
• Kasetti RB, Maddineni P, Millar JC et al. Increased synthesis and deposition of extracellular matrix proteins leads to endoplasmic reticulum stress in the trabecular meshwork Sci Rep. 2017 Nov 02 [PMID: 29097767] (ICC/IF, Human)
• Joshi S, Wang W, Khan SR. Transcriptional study of hyperoxaluria and calcium oxalate nephrolithiasis in male rats: Inflammatory changes are mainly associated with crystal deposition PLoS ONE. 2017 Nov 01 [PMID: 29091707] (IHC-P, Rat)
• Kaukonen R, Jacquemet G, Hamidi H, Ivaska J. Cell-derived matrices for studying cell proliferation and directional migration in a complex 3D microenvironment Nat Protoc. 2017 Nov 01 [PMID: 29048422] (ICC/IF)
• Lee DJ, Lee YT, Zou R et al. Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects. Sci Rep. 2017 Oct 11 [PMID: 29021583] (ICC/IF, Rat)
• Gai Z, Chu L, Xu Z et al. Farnesoid X receptor activation protects the kidney from ischemia-reperfusion damage Sci Rep 2017 Aug 29 [PMID: 28852062] (IHC-P, Mouse)
• Whatcott CJ, Ng S, Barrett MT et al. Inhibition of ROCK1 kinase modulates both tumor cells and stromal fibroblasts in pancreatic cancer PLoS ONE 2017 Aug 25 [PMID: 28841710] (IHC-P, Mouse)
• Chen X, Li W, Li Y et al. Elevated serum antibody against Schistosoma japonicum HSP60 as a promising biomarker for liver pathology in schistosomiasis Sci Rep 2017 Aug 10 [PMID: 28798366] (IHC-P, Mouse)
• Hino K, Horigome K, Nishio M et al. Activin-A enhances mTOR signaling to promote aberrant chondrogenesis in fibrodysplasia ossificans progressiva J. Clin. Invest. 2017 Jul 31 [PMID: 28758906] (IHC-P, Human)
• Doebar S C, Sieuwerts A M et al. Gene Expression Differences between Ductal Carcinoma in Situ with and without Progression to Invasive Breast Cancer. Am J Pathol Jan 7 2017 [PMID: 28634007] (IHC, Human)
• Tombulturk FK, Kasap M, Tuncdemir M et al. Effects of Lucilia sericata on wound healing in streptozotocin-induced diabetic rats and analysis of its secretome at the proteome level. Hum Exp Toxicol. 2017 Jan 01 [PMID: 28621210] (IHC)
• Mahdy MAA, Warita K, Hosaka YZ. Effects of transforming growth factor-B1 treatment on muscle regeneration and adipogenesis in glycerol-injured muscle. Anim. Sci. J. 2017 Jun 06 [PMID: 28585769]
• Haak AJ, Girtman MA, Ali MF et al. Phenylpyrrolidine structural mimics of pirfenidone lacking antifibrotic activity: A new tool for mechanism of action studies. Eur. J. Pharmacol. 2017 May 30 [PMID: 28576410] (Human)
• Pan R, Zhang Y, Zheng M et al. Hydroxysafflor Yellow A Suppresses MRC-5 Cell Activation Induced by TGF-b1 by Blocking TGF-b1 Binding to TbRII. Front Pharmacol May 29 2017 [PMID: 28553231] (ICC/IF, WB, Human)
• Lugenbiel P, Wenz F, Govorov K et al. Atrial myofibroblast activation and connective tissue formation in a porcine model of atrial fibrillation and reduced left ventricular function. Life Sci. May 22 2017 [PMID: 28546006] (Porcine)
• Gerarduzzi C, Kumar RK, Trivedi P et al. Silencing SMOC2 ameliorates kidney fibrosis by inhibiting fibroblast to myofibroblast transformation. JCI Insight. Apr 20 2017 [PMID: 28422762] (WB, Human)
• Hernandez H, Medina-Ortiz WE, Luan T et al. Crosstalk Between Transforming Growth Factor Beta-2 and Toll-Like Receptor 4 in the Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. Mar 1 2017 [PMID: 28346614] (ICC/IF, Human)
• Mi Y, Wang W et al. Autophagic Degradation of Collagen 1A1 by Cortisol in Human Amnion Fibroblasts. Endocrinology Jan 4 2017 [PMID: 28323983] (WB, Human)
• Liu R, Das B, Xiao W et al. A Novel Inhibitor of Homeodomain Interacting Protein Kinase 2 Mitigates Kidney Fibrosis through Inhibition of the TGF-b1/Smad3 Pathway. J. Am. Soc. Nephrol. 2017 Feb 20 [PMID: 28220029] (WB)
• Patel GC, Phan TN, Maddineni P et al. Dexamethasone-Induced Ocular Hypertension in Mice: Effects of Myocilin and Route of Administration. Am. J. Pathol. 2017 Feb 03 [PMID: 28167045]
• Sansalone Salvatore, Loreto Carla, Leonardi Rosario et al. Microsurgical tunica albuginea transplantation in an animal model. Asian Journal of Andrology [PMID: 28139472] (IHC, Rat)
• Townsend J, Dennis S, Whitlow J et al. Colloidal Gels with Extracellular Matrix Particles and Growth Factors for Bone Regeneration in Critical Size Rat Calvarial Defects. The AAPS Journal. [PMID: 28138909] (IHC, Rat)
• Hellewell AL, Rosini S, Adams JC. A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix J Vis Exp Jan 4 2017 [PMID: 28117783] (ICC/IF, Human)
• Malinverno M, Corada M, Ferrarini L et al. Peg3/PW1 is a marker of a subset of vessel associated endothelial progenitors Stem Cells Jan 16 2017 [PMID: 28090691] (ICC/IF, WB, Mouse)
• Lugenbiel P, Wenz F, Syren P et al. TREK-1 (K2P2.1) K(+) channels are suppressed in patients with atrial fibrillation and heart failure and provide therapeutic targets for rhythm control. Basic Res. Cardiol. Jan 1 2017 [PMID: 28005193] (Human)
• Peuhu E, Kaukonen R, Lerche M et al. SHARPIN regulates collagen architecture and ductal outgrowth in the developing mouse mammary gland. EMBO J. Dec 14 2016 [PMID: 27974362] (WB, Mouse)
• Kasetti RB, Phan TN, Millar JC, Zode GS. Expression of Mutant Myocilin Induces Abnormal Intracellular Accumulation of Selected Extracellular Matrix Proteins in the Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. Nov 1 2016 [PMID: 27820874] (Human)
• Makitsubo M, Adachi N, Nakasa T et al. Differences in joint morphology between the knee and ankle affect the repair of osteochondral defects in a rabbit model. J Orthop Surg Res. Oct 4 2016 [PMID: 27716360] (IHC, Rabbit)
• Cakir-Ozkan N, Eri S, Bekar E et al. The Use of Sequentially VEGF and BMP2 Releasing Biodegradable Scaffolds in Rabbit Mandibular Defects. J Oral Maxillofac Surg. Aug 27 2016 [PMID: 27663536] (IHC, Rabbit)
• Sakurai Y, Hada T, Yamamoto S et al. Remodeling of the Extracellular Matrix by Endothelial Cell-Targeting siRNA Improves the EPR-Based Delivery of 100 nm Particles. Mol Ther 2016 Dec [PMID: 27633444]
• WULANDARI E, JUSMAN SW, MOENADJAT Y et al. Expressions of Collagen I and III in Hypoxic Keloid Tissue Kobe J Med Sci. 2016 Jun 08 [PMID: 27604536]
• Kaukonen R, Mai A, Georgiadou M et al. Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription. Nat Commun. Aug 4 2016 [PMID: 27488962] (ICC/IF, Human)
• Lewis DM, Park KM, Tang V et al. Intratumoral oxygen gradients mediate sarcoma cell invasion. Proc. Natl. Acad. Sci. U.S.A. Aug 16 2016 [PMID: 27486245] (ICC/IF, Mouse)
• Granato M, Zompetta C, Vescarelli E et al. HCV derived from sera of HCV-infected patients induces pro-fibrotic effects in human primary fibroblasts by activating GLI2. Sci Rep. Aug 1 2016 [PMID: 27476557] (ICC/IF, Human)
• Banushi B, Forneris F, Straatman-Iwanowska A et al. Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis Nat Commun 2016 Jul 20 [PMID: 27435297] (IP)
• Xie T, Liang J, Liu N et al. Transcription factor TBX4 regulates myofibroblast accumulation and lung fibrosis J. Clin. Invest. 2016 Aug 01 [PMID: 27400124]
• Chen HJ, Wei Z, Sun J et al. A recellularized human colon model identifies cancer driver genes Nat. Biotechnol. 2016 Jul 11 [PMID: 27398792]
• Yang Jie, Xiang Fei, Cai Peng-Cheng et al. Activation of calpain by renin-angiotensin system in pleural mesothelial cells mediates tuberculous pleural fibrosis. Am J Physiol Lung Cell Mol Physiol 2016 [PMID: 27261452] (WB, Human)
• Lee DJ, Park Y, Hu WS, Ko CC. Osteogenic Potential of Multipotent Adult Progenitor Cells for Calvaria Bone Regeneration. Adv Med. May 30 2016 [PMID: 27239552] (IHC, Rat)
• Chen PY, Qin L, Li G et al. Smooth muscle FGF/TGFbeta cross talk regulates atherosclerosis progression. EMBO Mol Med. May 13 2016 [PMID: 27189169] (IHC, Mouse)
• Deng HW, Tian Y, Zhou XJ et al. Effect of Bilberry Extract on Development of Form-Deprivation Myopia in the Guinea Pig. J Ocul Pharmacol Ther 2016 May [PMID: 26982283]
• Hunziker EB, Lippuner K, Keel MJ et al. A novel organ-slice culturing system to simulate meniscal repair: Proof of concept using a synovium-based pool of meniscoprogenitor cells. J. Orthop. Res. 2016 Jan 21 [PMID: 26790377] (IHC-P, Human)
• Ying R, Wang XQ, Yang Y et al. Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway. Life Sci. 2016 Jan 01 [PMID: 26656263] (WB, Human)
• Chen PY, Qin L, Baeyens N et al. Endothelial-to-mesenchymal transition drives atherosclerosis progression. J Clin Invest 2015 Oct 26 [PMID: 26517696] (WB)
• Schmidt JB, Tranquillo RT. Cyclic Stretch and Perfusion Bioreactor for Conditioning Large Diameter Engineered Tissue Tubes. Ann Biomed Eng. 2015 Aug 26 [PMID: 26307332] (IHC-Fr, Human)
• Bertolo A, Arcolino F, Capossela S et al. Growth factors cross-linked to collagen microcarriers promote expansion and chondrogenic differentiation of human mesenchymal stem cells. Tissue Eng Part A. 2015 Jul 29 [PMID: 26222829] (Human)
• Jenkitkasemwong S, Wang CY, Coffey R et al. SLC39A14 Is Required for the Development of Hepatocellular Iron Overload in Murine Models of Hereditary Hemochromatosis. Cell Metab 2015 Jul 7 [PMID: 26028554] (WB)
• Rana Indrajeetsinh, Kompa Andrew R, Skommer Joanna et al. Contribution of microRNA to pathological fibrosis in cardio-renal syndrome: impact of uremic toxins. Physiol Rep 2015 [PMID: 25896982] (WB, Rat)
• Arias-Garcia M, Rickman R, Sero J The cell-cell adhesion protein JAM3 determines nuclear deformability by regulating microtubule organization biorxiv.org Jan 1 2019 (ICC/IF, Human)
• Cook J. Toward a Mechanistic Understanding of Hepatic Insulin Action and Resistance. Thesis. 2014 (WB, Mouse)
• Rodriguez-Rivera V. Novel Biofabrication Technologies to Recapitulate In Vivo Geometries in Collagen Hydrogels Thesis. 2014 Dec 15 (WB)
• Shetty R, Sathyanarayanamoorthy A, Ramachandra RA et al. Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity. Mol Vis 2015 [PMID: 25593510] (IHC)
• Weidenhamer NK, Moore DL, Lobo FL et al. Influence of culture conditions and extracellular matrix alignment on human mesenchymal stem cells invasion into decellularized engineered tissues. J Tissue Eng Regen Med. 2015 Jan 02 [PMID: 25556358]
• Ikezumi Y, Suzuki T, Yamada T et al. Alternatively activated macrophages in the pathogenesis of chronic kidney allograft injury. Pediatr. Nephrol. 2014 Dec 09 [PMID: 25487670] (IHC-P, Human)
  
  Details:
  Collagen I alpha 1 antibody used for IHC-P on human kidney allograft protocol biopsies (Fig 1)
• Duarte AS, Cavaleiro E, Pereira C et al. Aeromonas piscicola AH-3 expresses an extracellular collagenase with cytotoxic properties. Lett. Appl. Microbiol. 2014 Dec 02 [PMID: 25443157] (WB)
  
  Details:
  Collagen I alpha 1 antibody used for Far-Western blot analysis on cell-free supernatants (CFSs) from Aeromonas piscicola AH-3 and Aeromonas piscicola AH-3::colAh knockout mutants, with collagen type I as a positive control (Figure 1C).
• Gehmlich K, Dodd MS, William Allwood J et al. Changes in the cardiac metabolome caused by perhexiline treatment in a mouse model of hypertrophic cardiomyopathy. Mol Biosyst. 2014 Dec 01 [PMID: 25437646] (WB, Mouse)
  
  Details:
  Collagen I alpha 1 antibody used in WB for examining the Collagen 1 protein levels in perhexiline- and placebo-treated Mybpc3 KI mice compared to WT, with endogenous protein calsequestrin (CSQ) used as a normalization control. WB data is shown in Figure S4A.
• Fukuda M, Aoki T, Manabe T et al. Exacerbation of Intracranial Aneurysm and Aortic Dissection in Hypertensive Rat Treated With the Prostaglandin F-Receptor Antagonist AS604872. J. Pharmacol. Sci. 2014 Oct 23 [PMID: 25341845] (IHC, Human, Rat)
• Ramani K, Donoyan S, Tomasi Ml et al. Role of Methionine Adenosyltransferase Alpha2 and B Phosphorylation and Stabilization in Human Hepatic Stellate Cell Trans-Differentiation. J. Cell. Physiol. 2014 Oct 07 [PMID: 25294683] (WB, Human)
• Di Giancamillo A, Deponti D, Addis A et al. Meniscus maturation in the swine model: changes occurring along with anterior to posterior and medial to lateral aspect during growth. J Cell. Mol. Med. 2014 Sep 12 [PMID: 25216283]
• Elsner A,Lange F,Fitzner B et al. Distinct antifibrogenic effects of erlotinib, sunitinib and sorafenib on rat pancreatic stellate cells World J. Gastroenterol. 2014 Jun 28 [PMID: 24976727] (WB, Rat)
  
  Details:
  Collagen I antibody used for WB on protein extracts from rat's pancreatic stellate cells/ PSCs using PVDF membrane protein transfer and LI-COR -Odyssey detection. Figure 4A - type?I?collagen 150 kDa expression levels in Rat's PSCs treated or not with 48h of 10uM/L erlotinib, sorafenib or sunitinib.
• Alaghemand H, Esmaeili B, Firouz P. A comparative study of the preventive effect of chlorhexidine o. 12% and nano zinc oxide particles on the distraction of collagen scaffolding of the hybrid layer by two immunohistochemistry and microleakage tests. Dentistry and Medical research. 2014 Sep 21 (IHC-P, Human)
  
  Details:
  Collagen I alpha 1 antibody used for IHC-P on 4um sections of dentin extracted from human subject's molars and premolar teeth with no caries and visible anatomic defects (Figure 2).
• Torres PM, Vieira SI, Cerqueira AR et al. Effects of Mn-doping on the structure and biological properties of beta-tricalcium phosphate. J. Inorg. Biochem. 2014 Apr 03 [PMID: 24747361] (WB, ICC/IF, Mouse)
• Nijhuis A, Biancheri P, Lewis A et al. In Crohn's disease fibrosis reduced expression of the miR-29 family enhances collagen expression in intestinal fibroblasts. Clin. Sci. 3/18/2014 [PMID: 24641356] (ICC/IF, Human)
• Majumder K, Chakrabarti S, Morton JS et al. Egg-Derived Tri-Peptide IRW Exerts Antihypertensive Effects in Spontaneously Hypertensive Rats. PLoS One. 2013 Nov 29 [PMID: 24312436] (IHC-Fr, ICC/IF, Rat)
• Rebours V, Albuquerque M, Sauvanet A et al. Hypoxia Pathways and Cellular Stress Activate Pancreatic Stellate Cells: Development of an Organotypic Culture Model of Thick Slices of Normal Human Pancreas. PLoS One. 2013 Sep 30 [PMID: 24098783] (IHC-P, Human)
• Seet LF, Finger SN, Chu SW et al. Novel Insight into the Inflammatory and Cellular Responses Following Experimental Glaucoma Surgery: A Roadmap for Inhibiting Fibrosis. Curr Mol Med 2013 Jun 7 [PMID: 23651348]
• Gilkes DM, Chaturvedi P, Bajpai S et al. Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res 2013 Jun 1 [PMID: 23539444] (WB, Human)
• Ni J, Dong Z, Han W et al. The Role of RhoA and Cytoskeleton in Myofibroblast Transformation in Hyperoxic Lung Fibrosis Free Radic Biol Med 2013 Mar 18 [PMID: 23517783] (WB, IHC-P, ICC/IF, Human, Mouse)
• Gilkes DM, Bajpai S, Chaturvedi P et al. HIF-1 Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts. J Biol Chem 2013 Feb 19 [PMID: 23423382] (WB, Human)
• Gilkes DM, Bajpai S, Wong CC et al. Procollagen lysyl hydroxylase 2 is essential for hypoxia-induced breast cancer metastasis. Mol Cancer Res 2013 May [PMID: 23378577] (WB, Human)
• Masuzaki R, Zhao SR, Csizmadia E et al. Scar formation and lack of regeneration in adult and neonatal liver after stromal injury Wound Repair Regen 2013 Jan [PMID: 23228176] (IHC, Mouse)
• Chen PY, Qin L, Barnes C et al. FGF Regulates TGF-beta Signaling and Endothelial-to-Mesenchymal Transition via Control of let-7 miRNA Expression Cell Rep 2012 Nov 27 [PMID: 23200853] (IHC, ICC/IF, Mouse)
• Weidenhamer NK, Tranquillo RT. Influence of Cyclic Mechanical Stretch and Tissue Constraints on Cellular and Collagen Alignment in Fibroblast-derived Cell Sheets Tissue Eng Part C Methods 2012 Nov 6 [PMID: 23126441] (IHC, ICC/IF, Human)
• Kaukonen R. Filopodia and stromal extracellular matrix as regulators of cancer cell invasion and growth. Thesis. (ICC/IF, Human)
• Jobara Kanta, Kaido Toshimi, Hori Tomohide et al. Whey-hydrolyzed peptide-enriched immunomodulating diet prevents progression of liver cirrhosis in rats. Nutrition. 2014 Feb 28 [PMID: 24976428] (Rat)
• Wang B, Sun J, Qian S et al. Proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 film deposited by cathodic arc Biomed Pharmacother 2012 Sep 27 [PMID: 23089479] (ICC/IF, WB, Human)
• Seo H, Song Y, Heo J et al. Identification of Hepatic Fibrosis Inhibitors Through Morphometry Analysis of Hepatic Multicellular Spheroids Models Research Square Nov 17 2020 (WB, Human)
• Pratap A, Singh S, Mundra V et al. Attenuation of early liver fibrosis by pharmacological inhibition of smoothened receptor signaling J Drug Target 2012 Nov [PMID: 22994359] (WB, Rat)
• Zhang S, Sun J, Xu Y et al. Biological Behavior of Osteoblast-like Cells on Titania and Zirconia Films Deposited by Cathodic Arc Deposition Biointerphases 2012 Dec [PMID: 23055096] (ICC/IF, Human)
• Gokce A, Yilmaz I, Bircan R et al. Synergistic Effect of TGF-beta1 And BMP-7 on Chondrogenesis and Extracellular Matrix Synthesis: An In Vitro Study Open Orthop J 2012 [PMID: 23002411] (WB, Human)
• Geirsson A, Singh M, Ali R et al. Modulation of Transforming Growth Factor-beta Signaling and Extracellular Matrix Production in Myxomatous Mitral Valves by Angiotensin II Receptor Blockers Circulation 2012 Sep 11 [PMID: 22965982] (IHC, Human)
• Lai VK, Frey CR, Kerandi AM et al. Microstructural and Mechanical Differences Between Digested Collagen-Fibrin Co-Gels and Pure Collagen and Fibrin Gels Acta Biomater 2012 Jul 21 [PMID: 22828381] (ICC/IF, Rat)
• Lee HJ, Won JH, Doo SH et al. Inhibition of collagen deposit in obstructed rat bladder outlet by transplantation of superparamagnetic iron oxide-labeled human mesenchymal stem cells as monitored by molecular magnetic resonance imaging (MRI) Cell Transplant 2012 [PMID: 22449414] (WB, Rat)
• Zhou L, Wang L, Lu L, Jiang P, Sun H et al. Inhibition of miR-29 by TGF-beta-Smad3 Signaling through Dual Mechanisms Promotes Transdifferentiation of Mouse Myoblasts into Myofibroblasts PLoS ONE 2012 [PMID: 22438993] (WB, ICC/IF, Mouse)
• Remacle AG, Golubkov VS, Shiryaev SA, Dahl R, Stebbins JL, Chernov AV, Cheltsov AV, Pellecchia M, Strongin AY. Novel MT1-MMP Small-Molecule Inhibitors Based on Insights into Hemopexin Domain Function in Tumor Growth. Cancer Res;72(9):2339-49. 2012 May 1. [PMID: 22406620] (IHC, ICC/IF, Mouse)
• Ferreira JR, Hirsch ML, Zhang L et al. Three-dimensional multipotent progenitor cell aggregates for expansion, osteogenic differentiation and 'in vivo' tracing with AAV vector serotype 6 Gene Ther 2012 Mar 8 [PMID: 22402320] (IHC-Fr, Rat)
• Seet L-F, Tong L, Su R, Wong TT. Involvement of SPARC and MMP-3 in the Pathogenesis of Human Pterygium. Investigative Ophthalmology & Visual Science. 2012 Jan 4. [PMID: 22222271]
• Mak KM, Kwong AJ, Chu E et al. Hepatic steatosis, fibrosis, and cancer in elderly cadavers. Anat Rec (Hoboken) 2012 Jan [PMID: 22139908]
• Ma W, Han W, Greer PA et al. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease. The Journal of Clinical Investigation. 2011 Oct 17. [PMID: 22005303]
• Deng Y, Chen B, Qi Y et al. The effects of levofloxacin on rabbit anterior cruciate ligament cells in vitro. Toxicology and Applied Pharmacology. 2011 Aug 27. [PMID: 21903125]
• Golubkov VS, Chernov AV, Strongin AY. Intradomain cleavage of the inhibitory prodomain is essential to the pro-tumorigenic function of membrane type-1 matrix metalloproteinase (MT1-MMP) in vivo. J Biol Chem. 2011 Aug 8. [PMID: 21832072]
• Seet LF, Su R, Toh LZ, Wong TT. In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon's fibroblasts: comparisons with mitomycin-C. J Cell Mol Med. 2011 Jul 31. [PMID: 21801304] (WB, Human)
• Lee K, Kim H, Kim JM et al. Nacre-driven water-soluble factors promote wound healing of the deep burn Porcine skin by recovering angiogenesis and fibroblast function. Mol Biol Rep. 2011 Jun 19. [PMID: 21688145] (IHC, Porcine)
• Ahmann KA, Johnson SL, Hebbel RP, Tranquillo RT. Shear stress responses of adult blood outgrowth endothelial cells seeded on bioartificial tissue. Tissue Eng Part A;17(19-20):2511-21. 2011 Oct. [PMID: 21599543] (IHC, Human)
• Crawford JR, Pilling D, Gomer RH et al. Improved serum-free culture conditions for spleen-derived murine fibrocytes. J Immunol Methods 2010 Dec [PMID: 20888336]
• Ramirez Allan M, Wongtrakool Cherry, Welch Teresa et al. Vitamin D inhibition of pro-fibrotic effects of transforming growth factor beta1 in lung fibroblasts and epithelial cells. J Steroid Biochem Mol Biol 2010 [PMID: 19931390] (WB, Mouse)
• Han Weihong, Wang Wei, Mohammed Kamal A, Su Yunchao. Alpha-defensins increase lung fibroblast proliferation and collagen synthesis via the beta-catenin signaling pathway. FEBS J 2009 [PMID: 19814765] (WB, Human)
• Sotgia F, Del Galdo F, Casimiro MC et al. Caveolin-1-/- Null Mammary Stromal Fibroblasts Share Characteristics with Human Breast Cancer-Associated Fibroblasts. Am J Pathol;174(3):746-761. 2009 [PMID: 19234134]
• Keophiphath M, Achard V, Henegar C et al. Macrophage-Secreted Factors Promote a Profibrotic Phenotype in Human Preadipocytes. Mol Endocrinol;23(1):11-24. 2009 [PMID: 18945811]
• Sales, V L, Engelmayr, G C, Jr, Mettler, B A, Johnson, J A, Jr, Sacks, M S, Mayer, J E, Jr. Transforming Growth Factor-{beta}1 Modulates Extracellular Matrix Production, Proliferation, Apoptosis of Endothelial Progenitor Cells in Tissue-Engineering Scaffolds, 114, I-193-199. 2006 [PMID: 16820571]
• She H, Xiong S, Hazra S et al. Adipogenic transcriptional regulation of hepatic stellate cells. J Biol Chem 2005 Feb [PMID: 15537655]
• Hashimoto N, Jin H, Liu T et al. Bone marrow-derived progenitor cells in pulmonary fibrosis. J Clin Invest 2004 Jan [PMID: 14722616]
• Hazra S, Xiong S, Wang J et al. Peroxisome proliferator-activated receptor gamma induces a phenotypic switch from activated to quiescent hepatic stellate cells. J Biol Chem 2004 Mar [PMID: 14702344]
• Stefanovic B, Schnabl B, Brenner DA et al. Inhibition of collagen alpha 1(I) expression by the 5' stem-loop as a molecular decoy. J Biol Chem 2002 May [PMID: 11889120]

Reviews for Collagen I Antibody (NB600-408) (14)

Average Rating: 4.5

(Based on 14 reviews)

We have 14 reviews tested in 5 species: Human, Mouse, Rat, Feline, Other.

reviewed by: Anonymous IHC-P Mouse 10/10/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Pancreas
• Species: Mouse
• Lot: 42336

reviewed by: Anonymous IHC-P Mouse 10/02/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Balb/3T3 mouse embryonic fibroblast cell line
• Species: Mouse
• Lot: 40267

reviewed by: Wangsheng Wang WB Human 05/30/2018

Summary

• Application: Western Blot
• Sample Tested: whole cell extract
• Species: Human
• Lot: 35820

reviewed by: Anonymous IHC-P Mouse 10/25/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Lot: 38594

reviewed by: Anonymous WB Human 09/27/2017

Summary

• Application: Western Blot
• Sample Tested: Cell lysate from human trabecular meshwork
• Species: Human
• Lot: 38594

reviewed by: Anonymous ICC Feline 06/23/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Adult small intestine
• Species: Feline
• Lot: 38594

Comments

• Comments: 4% PFA fixed feline tissue

reviewed by: Jakob Townsend IHC-P Rat 02/20/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Bone
• Species: Rat
• Lot: 37789

reviewed by: Anonymous IHC-P Rat 07/20/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: rat colon
• Species: Rat
• Lot: 34015

reviewed by: Anonymous WB Other 08/04/2015

Summary

• Application: Western Blot
• Species: Other
• File: View PDF

reviewed by: Anonymous IF Human 10/02/2014

Summary

• Application: Immunofluorescence
• Sample Tested: primary human cardiac fibroblasts
• Species: Human
• Lot: 32011

Blocking

• Blocking Details: blocking step for 1 h with 2% bovine serum albumin in PBS at room temperature

Primary Anitbody

• Dilution Ratio: primary antibody diluted 1:00 in BSA/PBS, incubation at 4C overnight

Secondary Antibody

• Secondary Description: goat anti rabbit Alexa488
• Secondary Manufacturer Cat#: A-21222
• Secondary Concentration: 1:200

Details

• Detection Notes: confocal microscopy, Zeiss LSM710
• Fixation Details: 15 min fixation with 3% para-formaldehyde in PBS at room temp, permeabilization with 10% Triton-X 100 in PBS for 15 min
• Wash Description: washing steps with PBS, usually 3 times for 5 minutes

reviewed by: Elizabeth Rendina-Ruedy WB Mouse 08/05/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse Flushed Femur and whole cell lysate MC3T3
• Species: Mouse
• Comments: Antibody was easily used to detect 4 forms of type 1 collagen in the mouse femur. Sometimes this would appear as a very large molecular weight smear, but considering the amount of collagen in bone and the various forms, we believe this was acceptable. Due to the large molecular weight we were using 4-20% gradient gels, which again helped us visualize all 4 forms.

Blocking

• Blocking Details: 5% NFDM in 0.05% TTBS for 1 hour at RT

Primary Anitbody

• Dilution Ratio: 1:1000 in 5% NFDM in 0.05% TTBS overnight at 4 degrees C

Secondary Antibody

• Secondary Description: Goast anti-rabbit, HRP conjugate
• Secondary Concentration: 1:20,000

Details

• Detection Notes: Super Signal Pico, Pierce

Comments

• Comments: Antibody was easily used to detect 4 forms of type 1 collagen in the mouse femur. Sometimes this would appear as a very large molecular weight smear, but considering the amount of collagen in bone and the various forms, we believe this was acceptable. Due to the large molecular weight we were using 4-20% gradient gels, which again helped us visualize all 4 forms.

reviewed by: Anonymous IHC-Fr Human 06/18/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: HaCaT-ras A5-RT3 (SCC) tumor sections
• Species: Human
• Comments: NB600-408 has worked perfectly for staining ColI in frozen tumor samples, always reproducible results

Secondary Antibody

• Secondary Description: anti-rabbit Cy-2 antibody (Dianova), host species: donkey, dilution 1:100 in 12% BSA

Details

• Detection Notes: IF Microscope
• Fixation Details: Fixation:5 min 80% Methanol @ RT, 2 min Aceton @ -20C.
• Wash Description: 1x dH2O, 3x 10 min PBS, 1x dH2O

Comments

• Comments: NB600-408 has worked perfectly for staining ColI in frozen tumor samples, always reproducible results

reviewed by: Nathan Weidenhamer ICC Human 02/04/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: human dermal fibroblast derived cell sheet
• Species: Human

Blocking

• Blocking Details: 5% normal donkey serum, 1 hr at room temp.

Primary Anitbody

• Dilution Ratio: 5 ug/ml in 5% normal donkey serum, overnight at 4 degrees C

Secondary Antibody

• Secondary Description: donkey anti-rabbit Cy3
• Secondary Manufacturer Cat#: Jackson ImmunoResearch, 711-165-152
• Secondary Concentration: 4 ug/ml in PBS

Details

• Fixation Details: 4% PFA, 1 hr at room temp.
• Wash Description: 3 x 10 min, PBS

reviewed by: Anonymous WB Human 09/04/2012

Summary

• Application: Western Blot
• Sample Tested: Fibroblast Cell Media
• Species: Human
• Comments: The antibody works well and seems to detect both N and C cleaved collagen 1 fragments (see blot) as well as the mature collagen form. Western blot was done under denaturing conditions so not restricted to non-denaturing gels. Antibody could be diluted to higher than 1:1000.

Blocking

• Blocking Details: 5% Milk in PBS-T, 20min, RT

Primary Anitbody

• Dilution Ratio: 1:1000 in 5% Milk in PBS-T, O/N, 4C

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG-HRP
• Secondary Concentration: 1:5000

Details

• Detection Notes: ECL Prime, 10 seconds

Comments

• Comments: The antibody works well and seems to detect both N and C cleaved collagen 1 fragments (see blot) as well as the mature collagen form. Western blot was done under denaturing conditions so not restricted to non-denaturing gels. Antibody could be diluted to higher than 1:1000.

FAQs for Collagen I Antibody (NB600-408). (Showing 1 - 2 of 2 FAQs).

1. Could you please advise if this antibody works for IHC-P application using bone tissues?
   • NB600-408 has been tested and validated for use in the following applications and species: Western blot, ELISA, IP and IHC-Paraffin in human, mouse, rat and bovine. We have not specifically tested bone tissue.
2. Do you follow standard Western procedures with this collagen Ab or can the protein not be denatured?
   • Since we have not posted any special instruction for using this antibody in WB on our web, you could use the standard WB protocol. Although this collagen I a 1 is a huge protein (Uniprot site), the structure is relatively simple; with few modified amino acids and glycosylations. The detection of this protein with SDS-PAGE (denaturing gel) will ensure the molecular size of the protein could be accurately detected and confirmed, even though the glycosylations may alter the true MW. The manufacture of NB600-408 was carried out by the injections of rabbit with the immunogens derived from native collagen Type I fragments derived from human and bovine placenta after the limited pepsin digestions and selective salt precipitations. Therefore this antibody should recognize the native, undenatured collagen 1 alpha 1. If using the undenatured lysates in WB, you should expect to detect a very large protein complexes on your gel/WB, as the collagen I a 1 interacts with itself and/or other components in the extracellular matrices; but our WB protocol should be able to serve the propose.

Bioinformatics

• Gene Symbol: COL1A1
• Entrez:
  • 1277(Human)
  • 29393(Rat)
  • 282187(Bovine)
• Uniprot:
  • P02452()
  • P02453(Bovine)