Collagen I Antibody

Images

 
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Rat colon tissue stained with Collagen I antibody (red) and Hoechst (blue). IHC-P image submitted by a verified customer review.
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Myofibroblast differentiation requires CD271/NGF activation. Representative alphaSMA (red) and COLLAGEN 1 (red) ...read more
Western Blot: Collagen I Antibody [NB600-408] - Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight 649 ...read more
Western Blot: Collagen I Antibody [NB600-408] - Representative Western blot image for Collagen I, Collagen 2 and GAPDH. N/group = 8. Image collected and cropped by CiteAb from the following publication ...read more
Immunohistochemistry: Collagen I Antibody [NB600-408] - Macro- and micro-architecture of human adipose tissue lobule. Representative immunostaining of the human AT lobule with Collagen I (COL1), CD34, and DAPI. The ...read more
Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Primary human cardiac fibroblast cells were stained with anti-Collagen I antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf ...read more
Western Blot: Collagen I Antibody [NB600-408] - Cell lysate from human trabecular meshwork. Antibody at 1:1.000. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Staining of human HaCaT-ras A5-RT3 (SCC) frozen tumor sections. Image provided by Dr. Wa'el Al Rawashdeh of RWTH Aachen University.
Immunohistochemistry: Collagen I Antibody [NB600-408] - Patient-derived CAF and NF CDMs are architecturally and functionally distinct. Representative western blots showing Collagen I (red) and fibronectin (blue) ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of Collagen I Antibody on alcohol-fixed rat lung tissue. Primary antibody dilution 1:200. Image from verified customer review.
Western Blot: Collagen I Antibody [NB600-408] - Analysis in porcine burn wound lysate using anti-Collagen I alpha 1 antibody. WB image submitted by a verified customer review.
Western Blot: Collagen I Antibody [NB600-408] - Detection of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Balb/3T3 mouse embryonic fibroblast cell line stained with Collagen I antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Mouse pancreas tissue stained with Collagen I antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Human lung tissue section stained with Collagen I antibody at 1:400.
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - FFPE right lobe of the liver tissue section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat ...read more
Immunohistochemistry: Collagen I Antibody [NB600-408] - Analysis of FFPE human lung. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin at pH 6. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin tissue at pH9. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: ...read more
Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Imaging of rat calvarial defect bone. IHC-P image submitted by a verified customer review.
Immunohistochemistry: Collagen I Antibody [NB600-408] - Immunohistological staining of EG treated rat kidneys for Collagen. Magnification X40, Scale bar 100 um. Image collected and cropped by CiteAb from the following ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RbSpecies Glossary
Applications WB, ELISA, FLISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PAGE, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Collagen I Antibody Summary

Immunogen
Collagen I from human and bovine placenta (Uniprot: P02452)
Localization
Extracellular matrix
Specificity
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL1A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:50000
  • Fluorophore-linked immunosorbent assay 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Knockdown Validated
  • Microarray
  • SDS-Page
  • Western Blot 1:1000-1:10000
Application Notes
This product has been tested by dot blot and IHC and are useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, Immunofluorescence, FLOW, and western blotting for highly sensitive qualitative analysis.

Use in SDS-Page reported in scientific literature (PMID: 30914477).
Reviewed Applications
Read 16 Reviews rated 4.6
using
NB600-408 in the following applications:

Publications
Read Publications using
NB600-408 in the following applications:

  • ICC
    1 publication
  • 44 publications
  • IF
    1 publication
  • IHC
    43 publications
  • 8 publications
  • 26 publications
  • IP
    1 publication
  • 1 publication
  • 1 publication
  • WB
    65 publications

Reactivity Notes

This antibody reacts with human, bovine, and most mammalian Type I collagens with negligible cross-reactivity with Type II, III, IV, V or VI collagens
Use in Mouse reported in scientific literature (PMID: 33758176).

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Collagen I Antibody

  • Alpha-1 type I collagen
  • alpha1(I) procollagen
  • CAFYD
  • COL1A1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain preproprotein
  • collagen alpha-1(I) chain
  • Collagen I
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • EDSARTH1
  • EDSC
  • OI1
  • OI2
  • OI3
  • OI4
  • pro-alpha-1 collagen type 1
  • type I proalpha 1
  • Type I Procollagen Alpha 1 Chain

Background

Collagen I is one of the most abundant extracellular matrix (ECM) proteins in mammals and is an important structural component in connective tissues, bones, teeth, skin, heart, and lungs (1, 2). Collagen I is commonly used for biomedical purposes including providing an ECM microenvironment for cell attachment, proliferation, and mesenchymal stem cell growth and differentiation to the osteogenic lineage (1, 3, 4). Furthermore, type I Collagen hydrogels are a promising scaffold for engineering tissues and tumors (4). Collagen type I can be extracted from numerous sources including mammals, most commonly bovine and porcine, fish, amphibians, and birds (1, 4). Structurally, Collagen I is a heterotrimer comprised of two alpha-1 chains and one alpha-2 chain that together forms a triple helix (1, 3, 5). Each alpha chain is primarily formed by glycine, proline, and hydroxyproline repeats with the alpha-1 chains having a theoretical molecular weight of 139 kDa and 129 kDa for the alpha-2 chain (3). The dimeric form of Collagen I has a theoretical molecular weight of ~270 kDa while the trimeric form is >400 kDa (3, 5).

A variety of disease pathologies have been associated with mutations in or disruptions in collagen type I. Osteogenesis imperfecta, also known as brittle bone disease, is caused by autosomal dominant mutations in Collagen type I encoding genes and characterized by fragile bones and skeletal deformities (6). Ehlers-Danlos syndromes (EDS) is another disorder that affects soft connective tissue and its various clinical subtypes are associated with different mutations in Collagen I (7).

Alternative names for Collagen I includes alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, and type I procollagen alpha 1 chain.

References

1. Chowdhury, S. R., Mh Busra, M. F., Lokanathan, Y., Ng, M. H., Law, J. X., Cletus, U. C., & Binti Haji Idrus, R. (2018). Collagen Type I: A Versatile Biomaterial. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-13-0947-2_21

2. Kisling, A., Lust, R. M., & Katwa, L. C. (2019). What is the role of peptide fragments of collagen I and IV in health and disease?. Life sciences. https://doi.org/10.1016/j.lfs.2019.04.042

3. Kwon, D., Kang, G. S., Han, D. K., Park, K., Kim, J. H., & Lee, S. H. (2014). Establishment and characterization of human engineered cells stably expressing large extracellular matrix proteins. Archives of pharmacal research. https://doi.org/10.1007/s12272-013-0294-0

4. Antoine, E. E., Vlachos, P. P., & Rylander, M. N. (2014). Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport. Tissue engineering. Part B, Reviews. https://doi.org/10.1089/ten.TEB.2014.0086

5. Leon-Lopez, A., Morales-Penaloza, A., Martinez-Juarez, V. M., Vargas-Torres, A., Zeugolis, D. I., & Aguirre-alvarez, G. (2019). Hydrolyzed Collagen-Sources and Applications. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules24224031

6. Marini, J. C., Forlino, A., Bachinger, H. P., Bishop, N. J., Byers, P. H., Paepe, A., Fassier, F., Fratzl-Zelman, N., Kozloff, K. M., Krakow, D., Montpetit, K., & Semler, O. (2017). Osteogenesis imperfecta. Nature reviews. Disease primers. https://doi.org/10.1038/nrdp.2017.52

7. Callewaert, B., Malfait, F., Loeys, B., & De Paepe, A. (2008). Ehlers-Danlos syndromes and Marfan syndrome. Best practice & research. Clinical rheumatology, 22(1), 165-189. https://doi.org/10.1016/j.berh.2007.12.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen I Antibody (NB600-408)(237)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bacteria, Bovine, Porcine, Rabbit.

We have publications tested in 10 applications: ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PAGE, WB.


Filter By Application
ICC
(1)
ICC/IF
(44)
IF
(1)
IHC
(43)
IHC-Fr
(8)
IHC-P
(26)
IP
(1)
MiAr
(1)
PAGE
(1)
WB
(65)
All Applications
Filter By Species
Human
(80)
Mouse
(53)
Rat
(30)
Bacteria
(1)
Bovine
(1)
Porcine
(6)
Rabbit
(4)
All Species
Showing Publications 1 - 10 of 237. Show All 237 Publications.
Publications using NB600-408 Applications Species
Lewis A, SAnchez S, Berti G et al. Small-molecule Wnt inhibitors are a potential novel therapy for intestinal fibrosis in Crohns disease Clinical science (London, England : 1979) Oct 14 2022 [PMID: 36156078] (ICC/IF, Human) ICC/IF Human
Paul K, Darzi S, Del Borgo M et al. Vaginal delivery of tissue engineered endometrial mesenchymal stem/stromal cells in an aloe vera-alginate hydrogel alleviates maternal simulated birth injury Applied Materials Today Mar 1 2021 (ICC/IF, Human) ICC/IF Human
Mi YB, Liu XH, Wang WS et al. ER-phagy Is Involved in the Degradation of Collagen I by IL-1B in Human Amnion in Parturition Journal of immunology (Baltimore, Md. : 1950) Oct 26 2022 [PMID: 36288908] (WB, Human) WB Human
Gong H, Chen L, He Y et al. Pleural thickening induced by Glaesserella parasuis infection was linked to increased collagen and elastin Frontiers in cellular and infection microbiology Aug 11 2022 [PMID: 36034702] (IHC-P, Porcine) IHC-P Porcine
Zhou YH, Guo Y, Zhu JY et al. Spheroid co-culture of BMSCs with osteocytes yields ring-shaped bone-like tissue that enhances alveolar bone regeneration Scientific reports Aug 27 2022 [PMID: 36030312] (IHC-P, Mouse)

Details:
IHC-P 1:100
IHC-P Mouse
Caporarello N, Lee J, Pham TX et al. Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis Nature communications Jul 25 2022 [PMID: 35879310] (WB, Human) WB Human
Hadjisavva R, Anastasiou O, Ioannou PS et al. Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition Cell reports Jul 19 2022 [PMID: 35858563] (ICC, Human) ICC Human
Li X, Yong T, Wei Z et al. Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblasts Nature communications May 19 2022 [PMID: 35589680] (IHC, Human, Mouse) IHC Human, Mouse
Xue W, Yu SY, Kuss M et al. 3D bioprinted white adipose model forin vitrostudy of cancer-associated cachexia induced adipose tissue remodeling Biofabrication May 26 2022 [PMID: 35504266]
Park HH, Kim YM, Anh Hong LT et al. Dual-functional hydrogel system for spinal cord regeneration with sustained release of arylsulfatase B alleviates fibrotic microenvironment and promotes axonal regeneration Biomaterials May 1 2022 [PMID: 35461098] (IHC, Rat) IHC Rat
Show All 237 Publications.

Reviews for Collagen I Antibody (NB600-408) (16) 4.616

Average Rating: 4.6
(Based on 16 reviews)
We have 16 reviews tested in 5 species: Human, Mouse, Rat, Feline, Other.

Reviews using NB600-408:
Filter by Applications
IHC-P
(6)
ICC
(3)
WB
(5)
IF
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(7)
Mouse
(4)
Rat
(3)
Feline
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Collagen I NB600-408
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5
reviewed by:
Verified Customer
IHC-P Rat 05/05/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRat Lung Tissue
SpeciesRat
Lot47500
Immunocytochemistry Collagen I NB600-408
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5
reviewed by:
Kannan Govindaraj
ICC Human 04/23/2021
View

Summary

ApplicationImmunocytochemistry
Sample Testedchondrocytes
SpeciesHuman
Lot44222
Immunohistochemistry-Paraffin Collagen I NB600-408
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5
reviewed by:
Daisy Dai
IHC-P Mouse 10/10/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
Lot42336
Immunohistochemistry-Paraffin Collagen I NB600-408
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4
reviewed by:
Nin Wan
IHC-P Mouse 10/02/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBalb/3T3 mouse embryonic fibroblast cell line
SpeciesMouse
Lot40267
  5
reviewed by:
Verified Customer
WB Human 05/30/2018
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell extract
SpeciesHuman
Lot35820
Immunohistochemistry-Paraffin Collagen I NB600-408
Enlarge
3
reviewed by:
Serina Ng
IHC-P Mouse 10/25/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedIHC on parrafin sections (mouse)
SpeciesMouse
Lot38594
Western Blot Collagen I NB600-408
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5
reviewed by:
MIN KANG
WB Human 09/27/2017
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate from human trabecular meshwork
SpeciesHuman
Lot38594
  4
reviewed by:
Kazuya Oikawa
ICC Feline 06/23/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedAdult small intestine
SpeciesFeline
Lot38594

Comments

Comments4% PFA fixed feline tissue
Immunohistochemistry-Paraffin Collagen I NB600-408
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5
reviewed by:
Verified Customer
IHC-P Rat 02/20/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBone
SpeciesRat
Lot37789
Immunohistochemistry-Paraffin Collagen I NB600-408
Enlarge
5
reviewed by:
Sandra Lourenssen
IHC-P Rat 07/20/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedrat colon
SpeciesRat
Lot34015
Western Blot Collagen I NB600-408
Enlarge
4
reviewed by:
Liyong Zhang
WB Other 08/04/2015
View

Summary

ApplicationWestern Blot
SpeciesOther
Immunofluorescence Collagen I NB600-408
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4
reviewed by:
Christian Zuppinger
IF Human 10/02/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedprimary human cardiac fibroblasts
SpeciesHuman
Lot32011
Western Blot Collagen I NB600-408
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4
reviewed by:
Verified Customer
WB Mouse 08/05/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse Flushed Femur and whole cell lysate MC3T3
SpeciesMouse
Immunohistochemistry-Frozen Collagen I NB600-408
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5
reviewed by:
Wa'el Al Rawashdeh
IHC-Fr Human 06/18/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedHaCaT-ras A5-RT3 (SCC) tumor sections
SpeciesHuman
Immunocytochemistry Collagen I NB600-408
Enlarge
5
reviewed by:
Verified Customer
ICC Human 02/04/2013
View

Summary

ApplicationImmunocytochemistry
Sample Testedhuman dermal fibroblast derived cell sheet
SpeciesHuman
Western Blot Collagen I NB600-408
Enlarge
5
reviewed by:
Daniele Gilkes
WB Human 09/04/2012
View

Summary

ApplicationWestern Blot
Sample TestedFibroblast Cell Media
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen I Antibody (NB600-408). (Showing 1 - 2 of 2 FAQs).

  1. Could you please advise if this antibody works for IHC-P application using bone tissues?
    • NB600-408 has been tested and validated for use in the following applications and species: Western blot, ELISA, IP and IHC-Paraffin in human, mouse, rat and bovine. We have not specifically tested bone tissue.
  2. Do you follow standard Western procedures with this collagen Ab or can the protein not be denatured?
    • Since we have not posted any special instruction for using this antibody in WB on our web, you could use the standard WB protocol. Although this collagen I a 1 is a huge protein (Uniprot site), the structure is relatively simple; with few modified amino acids and glycosylations. The detection of this protein with SDS-PAGE (denaturing gel) will ensure the molecular size of the protein could be accurately detected and confirmed, even though the glycosylations may alter the true MW. The manufacture of NB600-408 was carried out by the injections of rabbit with the immunogens derived from native collagen Type I fragments derived from human and bovine placenta after the limited pepsin digestions and selective salt precipitations. Therefore this antibody should recognize the native, undenatured collagen 1 alpha 1. If using the undenatured lysates in WB, you should expect to detect a very large protein complexes on your gel/WB, as the collagen I a 1 interacts with itself and/or other components in the extracellular matrices; but our WB protocol should be able to serve the propose.

Secondary Antibodies

 

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Recent Reviews

4.6
5
10
4
5
3
1
2
0
1
0

Verified Customer
05/05/2022
Application: IHC-P
Species: Rat

Kannan Govindaraj
04/23/2021
Application: ICC
Species: Human

Daisy Dai
10/10/2019
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol COL1A1
Entrez
Uniprot