Collagen I Antibody

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Rat colon tissue stained with Collagen I antibody (red) and Hoechst (blue). IHC-P image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Myofibroblast differentiation requires CD271/NGF activation. Representative alphaSMA (red) and COLLAGEN 1 (red) immunostaining with DAPI (blue) in non-treated (control), TGFbeta-treated cells with or without gamma-secretase inhibitor. Scale bars: 50 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY licence.

Western Blot: Collagen I Antibody [NB600-408] - Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.

Western Blot: Collagen I Antibody [NB600-408] - Representative Western blot image for Collagen I, Collagen 2 and GAPDH. N/group = 8. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.12367) licensed under a CC-BY licence.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Macro- and micro-architecture of human adipose tissue lobule. Representative immunostaining of the human AT lobule with Collagen I (COL1), CD34, and DAPI. The position of the septa is underlined, scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Primary human cardiac fibroblast cells were stained with anti-Collagen I antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf serum. ICC/IF image submitted by a verified customer review.

Western Blot: Collagen I Antibody [NB600-408] - Cell lysate from human trabecular meshwork. Antibody at 1:1.000. WB image submitted by a verified customer review.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Patient-derived CAF and NF CDMs are architecturally and functionally distinct. Representative western blots showing Collagen I (red) and fibronectin (blue) staining of patient #1 and #3 NF and CAF CDM. Scale bar, 20 um. (f) Representative SEM images of TIFF, Patient #1 NF and CAF CDM. Scale bar, 5 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms12237) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Staining of human HaCaT-ras A5-RT3 (SCC) frozen tumor sections. Image provided by Dr. Wa'el Al Rawashdeh of RWTH Aachen University.

Western Blot: Collagen I Antibody [NB600-408] - Analysis in porcine burn wound lysate using anti-Collagen I alpha 1 antibody. WB image submitted by a verified customer review.

Western Blot: Collagen I Antibody [NB600-408] - Detection of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2-2 ug/10 mL in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% non-fat milk. Detection occurred by incubation with a HRP conjugated secondary antibody at 1 ug/10 mL. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Balb/3T3 mouse embryonic fibroblast cell line stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Mouse pancreas tissue stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Human lung tissue section stained with Collagen I antibody at 1:400.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - FFPE right lobe of the liver tissue section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Analysis of FFPE human lung. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin at pH 6. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. FFPE Human Skin tissue at pH9. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Imaging of rat calvarial defect bone. IHC-P image submitted by a verified customer review.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Immunohistological staining of EG treated rat kidneys for Collagen. Magnification X40, Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0185009), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Po, Ba, Bv, Rb, Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, FLISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PAGE
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Collagen I Antibody Summary

• Description: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Type-I collagen is the predominant form of collagen in the human body and plays a role in scar tissue formation. Collagen type 1 is also an important component of tendons and found in most connective tissues including cartilage. Collagen 1 is associated with several diseases including: Ehlers Danlos syndrome (arthrochalasia), Ehlers-Danlos syndrome (classical type), Osteogenesis imperfecta type 1-4 and osteoporosis.
• Immunogen: Collagen I from human and bovine placenta (Uniprot: P02452)
• Localization: Extracellular matrix
• Specificity: Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Predicted Species: Human (100%), Mouse (92%), Rat (92%), Canine (98%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:50000
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Microarray
  • SDS-Page
  • Western Blot 1:1000-1:10000
• Application Notes: Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, Immunofluorescence, FLOW, and western blotting for highly sensitive qualitative analysis.
  Use in SDS-Page reported in scientific literature (PMID: 30914477).
• Theoretical MW: 139 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody reacts with human, bovine, and most mammalian Type I collagens with negligible cross-reactivity with Type II, III, IV, V or VI collagens
Use in Mouse reported in scientific literature (PMID: 33758176).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Collagen I Antibody

• Alpha-1 type I collagen
• alpha1(I) procollagen
• CAFYD
• COL1A1
• Collagen 1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain preproprotein
• collagen alpha-1(I) chain
• Collagen I
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• EDSARTH1
• EDSC
• OI1
• OI2
• OI3
• OI4
• pro-alpha-1 collagen type 1
• type I proalpha 1
• Type I Procollagen Alpha 1 Chain

Background

Collagen I is one of the most abundant extracellular matrix (ECM) proteins in mammals and is an important structural component in connective tissues, bones, teeth, skin, heart, and lungs (1, 2). Collagen I is commonly used for biomedical purposes including providing an ECM microenvironment for cell attachment, proliferation, and mesenchymal stem cell growth and differentiation to the osteogenic lineage (1, 3, 4). Furthermore, type I Collagen hydrogels are a promising scaffold for engineering tissues and tumors (4). Collagen type I can be extracted from numerous sources including mammals, most commonly bovine and porcine, fish, amphibians, and birds (1, 4). Structurally, Collagen I is a heterotrimer comprised of two alpha-1 chains and one alpha-2 chain that together forms a triple helix (1, 3, 5). Each alpha chain is primarily formed by glycine, proline, and hydroxyproline repeats with the alpha-1 chains having a theoretical molecular weight of 139 kDa and 129 kDa for the alpha-2 chain (3). The dimeric form of Collagen I has a theoretical molecular weight of ~270 kDa while the trimeric form is >400 kDa (3, 5).

A variety of disease pathologies have been associated with mutations in or disruptions in collagen type I. Osteogenesis imperfecta, also known as brittle bone disease, is caused by autosomal dominant mutations in Collagen type I encoding genes and characterized by fragile bones and skeletal deformities (6). Ehlers-Danlos syndromes (EDS) is another disorder that affects soft connective tissue and its various clinical subtypes are associated with different mutations in Collagen I (7).

Alternative names for Collagen I includes alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, and type I procollagen alpha 1 chain.

References

1. Chowdhury, S. R., Mh Busra, M. F., Lokanathan, Y., Ng, M. H., Law, J. X., Cletus, U. C., & Binti Haji Idrus, R. (2018). Collagen Type I: A Versatile Biomaterial. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-13-0947-2_21

2. Kisling, A., Lust, R. M., & Katwa, L. C. (2019). What is the role of peptide fragments of collagen I and IV in health and disease?. Life sciences. https://doi.org/10.1016/j.lfs.2019.04.042

3. Kwon, D., Kang, G. S., Han, D. K., Park, K., Kim, J. H., & Lee, S. H. (2014). Establishment and characterization of human engineered cells stably expressing large extracellular matrix proteins. Archives of pharmacal research. https://doi.org/10.1007/s12272-013-0294-0

4. Antoine, E. E., Vlachos, P. P., & Rylander, M. N. (2014). Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport. Tissue engineering. Part B, Reviews. https://doi.org/10.1089/ten.TEB.2014.0086

5. Leon-Lopez, A., Morales-Penaloza, A., Martinez-Juarez, V. M., Vargas-Torres, A., Zeugolis, D. I., & Aguirre-alvarez, G. (2019). Hydrolyzed Collagen-Sources and Applications. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules24224031

6. Marini, J. C., Forlino, A., Bachinger, H. P., Bishop, N. J., Byers, P. H., Paepe, A., Fassier, F., Fratzl-Zelman, N., Kozloff, K. M., Krakow, D., Montpetit, K., & Semler, O. (2017). Osteogenesis imperfecta. Nature reviews. Disease primers. https://doi.org/10.1038/nrdp.2017.52

7. Callewaert, B., Malfait, F., Loeys, B., & De Paepe, A. (2008). Ehlers-Danlos syndromes and Marfan syndrome. Best practice & research. Clinical rheumatology, 22(1), 165-189. https://doi.org/10.1016/j.berh.2007.12.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen I Antibody (NB600-408)(219)

Publications using NB600-408

• Keller CR, Hu Y, Ruud KF Et al. Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells Cancers Nov 22 2021 [PMID: 34831010] (ICC/IF, Human)
• Kasetti RB, Maddineni P, Kodati B Et al. Astragaloside IV Attenuates Ocular Hypertension in a Mouse Model of TGF beta 2 Induced Primary Open Angle Glaucoma International journal of molecular sciences Nov 19 2021 [PMID: 34830390] (ICC/IF, Human)
• de Sousa BM, Correia CR, Ferreira JAF Et al. Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants NPJ Regenerative medicine Nov 23 2021 [PMID: 34815414] (WB, Mouse)
• Lee J, Ung A, Kim H Et al. Engineering liver microtissues to study the fusion of HepG2 with mesenchymal stem cells and invasive potential of fused cells Biofabrication Nov 30 2021 [PMID: 34740205]
• Miura Y, Lam M, Bourke JE, Kanazawa S Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia Life science alliance Jan 1 2022 [PMID: 34728556] (IHC-P, Mouse)
• Huang LT, Chou HC, Chen CM. Inhibition of FABP4 attenuates hyperoxia-induced lung injury and fibrosis via inhibiting TGF- beta signaling in neonatal rats Journal of cellular physiology Oct 28 2021 [PMID: 34708870] (Rat)
• Maddineni P, Kasetti RB, Kodati B et al. Sodium 4-Phenylbutyrate Reduces Ocular Hypertension by Degrading Extracellular Matrix Deposition via Activation of MMP9 International journal of molecular sciences Sep 18 2021 [PMID: 34576258] (ICC/IF, Mouse)
• Chen M, Wen X, Gao Y et al. IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury International immunopharmacology Sep 20 2021 [PMID: 34555644] (WB, Mouse)
• Zhao S, Li W, Yu W et al. Exosomal miR-21 from tubular cells contributes to renal fibrosis by activating fibroblasts via targeting PTEN in obstructed kidneys Theranostics Aug 2 2021 [PMID: 34522205] (WB, Mouse)
• Zhu L, Xu F, Kang X et al. The antioxidant N-acetylcysteine promotes immune response and inhibits epithelial-mesenchymal transition to alleviate pulmonary fibrosis in chronic obstructive pulmonary disease by suppressing the VWF/p38 MAPK axis Molecular medicine (Cambridge, Mass.) Sep 3 2021 [PMID: 34479474] (WB, Rat)
• Maity P, Singh K, Krug L Et al. Persistent JunB activation in fibroblasts disrupts stem cell niche interactions enforcing skin aging Cell reports Aug 31 2021 [PMID: 34469740] (ICC/IF, Mouse)
• Zhang S, Wei C, Wang Q Et Al. M2-Polarized Macrophages Mediate Wound Healing by Regulating Connective Tissue Growth Factor via AKT, ERK 1/2, and STAT 3 Signaling Pathways Research Square Jun 22 2021 [PMID: 34398425] (IHC)
• Montaldo C, Terri M, Riccioni V Et Al. Fibrogenic signals persist in DAA-treated HCV patients after sustained virological response Journal of hepatology Jul 13 2021 [PMID: 34271004]
• Giacalone D, Huang L, Tan S Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology Methods in molecular biology (Clifton, N.J.) Jul 8 2021 [PMID: 34235663]
• Xie T, Kulur V, Liu N et al. Mesenchymal growth hormone receptor deficiency leads to failure of alveolar progenitor cell function and severe pulmonary fibrosis Science advances Jun 1 2021 [PMID: 34108218] (IHC, Mouse)
• Ishii H, Kaneko S, Yanai K et al. MicroRNA Expression Profiling in Diabetic Kidney Disease Translational research : the journal of laboratory and clinical medicine Jun 6 2021 [PMID: 34102327] (IHC, Mouse)
• Song Y, Kim S, Heo J et al. Identification of hepatic fibrosis inhibitors through morphometry analysis of a hepatic multicellular spheroids model Scientific reports May 25 2021 [PMID: 34035369] (WB, Human)
• Ouyang L, Su X, Li W et al. ALKBH1-demethylated DNA N6-methyladenine modification triggers vascular calcification via osteogenic reprogramming in chronic kidney disease The Journal of clinical investigation May 18 2021 [PMID: 34003800] (WB, Human)
• Stevenson NL, Bergen DJM, Lu Y et al. Giantin is required for intracellular N-terminal processing of type I procollagen The Journal of cell biology Jun 7 2021 [PMID: 33944912]
• Kwon T, Moon D, Kim J Et Al. Application of 630-nm and 850-nm Light-emitting Diodes and Microcurrent to Accelerate Collagen and Elastin Deposition in Porcine Skin Medical Lasers Jun 30 2021 (IHC-P)
• Ji S, Gumina D, McPeak K et al. Human placental villous stromal extracellular matrix regulates fetoplacental angiogenesis in severe fetal growth restriction Clinical science (London, England : 1979) Apr 27 2021 [PMID: 33904582]
• Suzuki K, Komura M, Obana K et al. A new method of primary engineering of esophagus using orthotopic in-body tissue architecture Journal of Pediatric Surgery Mar 1 2021 [PMID: 33845983]
• Li W, Qi N, Wang S et al. miR-455-5p regulates atrial fibrillation by targeting suppressor of cytokines signaling 3 Journal of physiology and biochemistry Apr 1 2021 [PMID: 33792885]
• Maier JI, Rogg M, HelmstAdter M et al. EPB41L5 controls podocyte extracellular matrix assembly by adhesome-dependent force transmission Cell reports Mar 23 2021 [PMID: 33761352] (ICC/IF, Human)
• Altera A, Tosi GM, Regoli M et al. The extracellular matrix complexity of idiopathic epiretinal membranes and the bilaminar arrangement of the associated internal limiting membrane in the posterior retina Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie Mar 24 2021 [PMID: 33760980]
• Li P, Liu Y, Qin X et al. SIRT1 attenuates renal fibrosis by repressing HIF-2 alpha Cell death discovery Mar 23 2021 [PMID: 33758176] (WB, Mouse)
• Cho HJ, Kim HJ, Lee K et al. Bioengineered Multicellular Liver Microtissues for Modeling Advanced Hepatic Fibrosis Driven Through Non-Alcoholic Fatty Liver Disease Small (Weinheim an der Bergstrasse, Germany) Mar 9 2021 [PMID: 33690979]
• Morimoto C, Takedachi M, Kawasaki K et al. Hypoxia stimulates collagen hydroxylation in gingival fibroblasts and periodontal ligament cells Journal of periodontology Mar 4 2021 [PMID: 33660864]
• Huang L, Zhang Y, Yang J et al. EGR 1 plays a protective role in AMPK inhibitor compound C induced apoptosis through ROS induced ERK activation in skin cancer cellsAnti fibrotic effects and the mechanism of action of miR 29c in silicosis Molecular medicine reports Apr 1 2021 [PMID: 33649785]
• Estermann M, Spiaggia G, Septiadi D et al. Design of Perfused PTFE Vessel-Like Constructs for In Vitro Applications Macromolecular bioscience Feb 24 2021 [PMID: 33624920] (IHC-P, Human)
• Rustenhoven J, Drieu A, Mamuladze T, et al. Functional characterization of the dural sinuses as a neuroimmune interface Cell Jan 18 2021 [PMID: 33508229]
• Wang H, Xu Y, Wang P, et al. Cell-mediated injectable blend hydrogel-BCP ceramic scaffold for in situ condylar osteochondral repair Acta biomaterialia Jan 13 2021 [PMID: 33453407] (IHC-P, Rabbit)
• Kim S, Hong HS Substance-P prevents the cholestatic liver injury by regulating inflammatory responses Peptides Jan 10 2021 [PMID: 33440226] (WB, Rat)
• Jian Z, Zhuang T, Qinyu T et al. 3D bioprinting of a biomimetic meniscal scaffold for application in tissue engineering Bioactive materials Jun 1 2021 [PMID: 33313450] (ICC/IF, Rabbit)
• O Shea TM, Wollenberg AL, Kim JH et al. Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions Nature communications Dec 4 2020 [PMID: 33277474] (IHC-Fr, Mouse)
• Mia MM, Cibi DM, Abdul Ghani SAB et al. YAP/TAZ deficiency reprograms macrophage phenotype and improves infarct healing and cardiac function after myocardial infarction PLoS biology Dec 1 2020 [PMID: 33264286] (IHC-Fr, Mouse)
  
  Details:
  Transgenic mouse hearts underwent immunohistochemical analysis.
• Kasetti RB, Patel PD, Maddineni P et al. ATF4 leads to glaucoma by promoting protein synthesis and ER client protein load Cell Stem Cell Oct 27 2020 [PMID: 33154371] (IHC-P)
• An M, Kim H, Moon JM et al. Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation Antioxidants (Basel, Switzerland) Sep 24 2020 [PMID: 32987843] (IHC, WB, Mouse)
• Dawson LA, Schanes PP, Marrero L et al. Proximal digit tip amputation initiates simultaneous blastema and transient fibrosis formation and results in partial regeneration Wound Repair Regen Aug 19 2020 [PMID: 32815252]
• Mietus CJ, Lackner TJ, Karvelis PS et al. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med Aug 8 2020 [PMID: 32784470] (ICC/IF, Bovine, Human)
• Esquibel CR, Wendt KD, Lee HC et al. Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue Front Neurosci Jul 7 2020 [PMID: 32733179]
• Yang H, An J, Choi I et al. Region-specific astrogliosis: differential vessel formation contributes to different patterns of astrogliosis in the cortex and striatum Mol Brain Jul 22 2020 [PMID: 32698847] (IHC, Mouse)
• Caporarello N, Meridew JA, Aravamudhan A et al. Vascular dysfunction in aged mice contributes to persistent lung fibrosis Aging Cell Jul 21 2020 [PMID: 32691484] (IHC, Human)
• Wang J, Li G, Hu L et al. Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells Stem Cells Int Jun 27 2020 [PMID: 32676119] (IHC-P, Porcine)
• Li X, Zhao J, Kasinath V et al. Lymph node fibroblastic reticular cells deposit fibrosis-associated collagen following organ transplantation J. Clin. Invest. Jun 29 2020 [PMID: 32597832] (IHC-Fr, Human)
• Gounani Z, Sen Karaman D, Venu AP et al. Coculture of P. aeruginosa and S. aureus on cell derived matrix - An in vitro model of biofilms in infected wounds J. Microbiol. Methods Jun 25 2020 [PMID: 32593628] (ICC/IF, Bacteria)
• Lee BJ, Hegewisch Solloa E, Shannon MJ, Mace EM Generation of cell-derived matrices that support human NK cell migration and differentiation J. Leukoc. Biol. May 11 2020 [PMID: 32392635] (ICC/IF, Human)
• Tran-Nguyen TK, Xue J, Feghali-Bostwick C et al. CD70 Activation Decreases Pulmonary Fibroblast Production of Extracellular Matrix Proteins Am. J. Respir. Cell Mol. Biol. Apr 22 2020 [PMID: 32320626]
• Kang Y, Roh MR, Rajadurai S et al. Hypoxia and HIF-1 alpha Regulate Collagen Production in Keloids J. Invest. Dermatol. Apr 18 2020 [PMID: 32315657]
• Kim J, Park M, Paek J et al. Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman's Syndrome Front. Physiol. Feb 14 2020 [PMID: 32116803] (IHC-Fr, Mouse)
• Purdy MP, Ducharme M, Haak AJ et al. YAP/TAZ are Activated by Mechanical and Hormonal Stimuli in Myometrium and Exhibit Increased Baseline Activation in Uterine Fibroids Reprod Sci Feb 13 2020 [PMID: 32056132]
• Khamar P, Nishtala K, Shetty R et al. Early biological responses in ocular tissue after SMILE and LASIK surgery Exp. Eye Res. Jan 27 2020 [PMID: 32001250] (IHC-P, Human)
• Canes L, Marti-Pamies I, Ballester-Servera C et al. Neuron-derived orphan receptor-1 modulates cardiac gene expression and exacerbates angiotensin II-induced cardiac hypertrophy Clin. Sci. Jan 27 2020 [PMID: 31985010]
• Minna E, Brich S, Todoerti K et al. Cancer Associated Fibroblasts and Senescent Thyroid Cells in the Invasive Front of Thyroid Carcinoma Cancers (Basel) Jan 1 2020 [PMID: 31906302] (IHC-P, IHC, Human)
• Regoli M, Tosi GM, Neri G The Peculiar Pattern of Type IV Collagen Deposition in Epiretinal Membranes J. Histochem. Cytochem. Dec 20 2019 [PMID: 31858878] (ICC/IF, Human)
• Liu Z, Qian M, Tang X, et al. Targeted regulation of fibroblast state by CRISPR-mediated CEBPA expression Respir. Res. Dec 11 2019 [PMID: 31829168] (ICC/IF, Human)
• Aljohani H, Senbanjo LT, Chellaiah MA Methylsulfonylmethane increases osteogenesis and regulates the mineralization of the matrix by transglutaminase 2 in SHED cells PLoS ONE Dec 5 2019 [PMID: 31805069] (WB, Human)
• Choi JH, Kim SM, Lee GH et al. Platyconic Acid A, Platycodi Radix-Derived Saponin, Suppresses TGF-1-induced Activation of Hepatic Stellate Cells via Blocking SMAD and Activating the PPAR Signaling Pathway Cells. Nov 29 2019 [PMID: 31795488] (WB, Rat)
• Fu C, Lu Y, Williams MA, et al. Emergency myelopoiesis contributes to immune cell exhaustion and pulmonary vascular remodeling Br. J. Pharmacol. Dec 3 2019 [PMID: 31793661] (WB, Mouse)
• Chen PY, Qin L, Li G et al. Endothelial TGF-beta signalling drives vascular inflammation and atherosclerosis Nat Metab Sep 1 2019 [PMID: 31572976] (IHC, Mouse)
• Benvenuto M, Mattera R, Miele MT et al. Effects of a natural multi-component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts Exp Ther Med [PMID: 31572512]
• Balasubramanian M, Hobson E, Skae M et al. Developing pathways to clarify pathogenicity of unclassified variants in Osteogenesis Imperfecta genetic analysis Mol Genet Genomic Med Sep 30 2019 [PMID: 31568717] (WB, ICC/IF, Human)
• Elrashidy RA, Liu G Long-term diabetes causes molecular alterations related to fibrosis and apoptosis in rat urinary bladder Exp. Mol. Pathol. Aug 31 2019 [PMID: 31479659]
• Vidoni C, Ferraresi A, Secomandi E et al. Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells Cell Commun. Signal Aug 19 2019 [PMID: 31426798] (WB, ICC/IF, Human)
• Sato K, Yoshizawa H, Seki T et al. Chemerin-9, a potent agonist of chemerin receptor (ChemR23), prevents atherogenesis Clin. Sci. Aug 30 2019 [PMID: 31399499] (WB, Human)
• Suo H, Li L, Zhang C et al. Glucosamine-grafted methacrylated gelatin hydrogels as potential biomaterials for cartilage repair J. Biomed. Mater. Res. Part B Appl. Biomater. Aug 1 2019 [PMID: 31369700]
• Guo Xiangjiang, Fereydooni Arash, Isaji Toshihiko et al. Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency. Scientific Reports 2019 Jul 30 [PMID: 31363142] (IHC-P, Mouse)
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  Details:
  Collagen I alpha 1 antibody used for IHC-P on human kidney allograft protocol biopsies (Fig 1)
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  Details:
  Collagen I alpha 1 antibody used for Far-Western blot analysis on cell-free supernatants (CFSs) from Aeromonas piscicola AH-3 and Aeromonas piscicola AH-3::colAh knockout mutants, with collagen type I as a positive control (Figure 1C).
• Gehmlich K, Dodd MS, William Allwood J et al. Changes in the cardiac metabolome caused by perhexiline treatment in a mouse model of hypertrophic cardiomyopathy. Mol Biosyst. 2014 Dec 01 [PMID: 25437646] (WB, Mouse)
  
  Details:
  Collagen I alpha 1 antibody used in WB for examining the Collagen 1 protein levels in perhexiline- and placebo-treated Mybpc3 KI mice compared to WT, with endogenous protein calsequestrin (CSQ) used as a normalization control. WB data is shown in Figure S4A.
• Fukuda M, Aoki T, Manabe T et al. Exacerbation of Intracranial Aneurysm and Aortic Dissection in Hypertensive Rat Treated With the Prostaglandin F-Receptor Antagonist AS604872. J. Pharmacol. Sci. 2014 Oct 23 [PMID: 25341845] (IHC, Human, Rat)
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  Details:
  Collagen I antibody used for WB on protein extracts from rat's pancreatic stellate cells/ PSCs using PVDF membrane protein transfer and LI-COR -Odyssey detection. Figure 4A - type?I?collagen 150 kDa expression levels in Rat's PSCs treated or not with 48h of 10uM/L erlotinib, sorafenib or sunitinib.
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• Lee HJ, Won JH, Doo SH et al. Inhibition of collagen deposit in obstructed rat bladder outlet by transplantation of superparamagnetic iron oxide-labeled human mesenchymal stem cells as monitored by molecular magnetic resonance imaging (MRI) Cell Transplant 2012 [PMID: 22449414] (WB, Rat)
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• Alaghemand H, Esmaeili B, Firouz P. A comparative study of the preventive effect of chlorhexidine o. 12% and nano zinc oxide particles on the distraction of collagen scaffolding of the hybrid layer by two immunohistochemistry and microleakage tests. Dentistry and Medical research. 2014 Sep 21 (IHC-P, Human)
  
  Details:
  Collagen I alpha 1 antibody used for IHC-P on 4um sections of dentin extracted from human subject's molars and premolar teeth with no caries and visible anatomic defects (Figure 2).
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Reviews for Collagen I Antibody (NB600-408) (15)

Average Rating: 4.5

(Based on 15 reviews)

We have 15 reviews tested in 5 species: Human, Mouse, Rat, Feline, Other.

reviewed by: Verified Customer ICC Human 04/23/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: chondrocytes
• Species: Human
• Lot: 44222

reviewed by: Verified Customer IHC-P Mouse 10/10/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Pancreas
• Species: Mouse
• Lot: 42336

reviewed by: Verified Customer IHC-P Mouse 10/02/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Balb/3T3 mouse embryonic fibroblast cell line
• Species: Mouse
• Lot: 40267

reviewed by: Wangsheng Wang WB Human 05/30/2018

Summary

• Application: Western Blot
• Sample Tested: whole cell extract
• Species: Human
• Lot: 35820

reviewed by: Verified Customer IHC-P Mouse 10/25/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Lot: 38594

reviewed by: Verified Customer WB Human 09/27/2017

Summary

• Application: Western Blot
• Sample Tested: Cell lysate from human trabecular meshwork
• Species: Human
• Lot: 38594

reviewed by: Verified Customer ICC Feline 06/23/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Adult small intestine
• Species: Feline
• Lot: 38594

Comments

• Comments: 4% PFA fixed feline tissue

reviewed by: Jakob Townsend IHC-P Rat 02/20/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Bone
• Species: Rat
• Lot: 37789

reviewed by: Verified Customer IHC-P Rat 07/20/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: rat colon
• Species: Rat
• Lot: 34015

reviewed by: Verified Customer WB Other 08/04/2015

Summary

• Application: Western Blot
• Species: Other
• File: View PDF

reviewed by: Verified Customer IF Human 10/02/2014

Summary

• Application: Immunofluorescence
• Sample Tested: primary human cardiac fibroblasts
• Species: Human
• Lot: 32011

Blocking

• Blocking Details: blocking step for 1 h with 2% bovine serum albumin in PBS at room temperature

Primary Anitbody

• Dilution Ratio: primary antibody diluted 1:00 in BSA/PBS, incubation at 4C overnight

Secondary Antibody

• Secondary Description: goat anti rabbit Alexa488
• Secondary Manufacturer Cat#: A-21222
• Secondary Concentration: 1:200

Details

• Detection Notes: confocal microscopy, Zeiss LSM710
• Fixation Details: 15 min fixation with 3% para-formaldehyde in PBS at room temp, permeabilization with 10% Triton-X 100 in PBS for 15 min
• Wash Description: washing steps with PBS, usually 3 times for 5 minutes

reviewed by: Elizabeth Rendina-Ruedy WB Mouse 08/05/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse Flushed Femur and whole cell lysate MC3T3
• Species: Mouse
• Comments: Antibody was easily used to detect 4 forms of type 1 collagen in the mouse femur. Sometimes this would appear as a very large molecular weight smear, but considering the amount of collagen in bone and the various forms, we believe this was acceptable. Due to the large molecular weight we were using 4-20% gradient gels, which again helped us visualize all 4 forms.

Blocking

• Blocking Details: 5% NFDM in 0.05% TTBS for 1 hour at RT

Primary Anitbody

• Dilution Ratio: 1:1000 in 5% NFDM in 0.05% TTBS overnight at 4 degrees C

Secondary Antibody

• Secondary Description: Goast anti-rabbit, HRP conjugate
• Secondary Concentration: 1:20,000

Details

• Detection Notes: Super Signal Pico, Pierce

Comments

• Comments: Antibody was easily used to detect 4 forms of type 1 collagen in the mouse femur. Sometimes this would appear as a very large molecular weight smear, but considering the amount of collagen in bone and the various forms, we believe this was acceptable. Due to the large molecular weight we were using 4-20% gradient gels, which again helped us visualize all 4 forms.

reviewed by: Verified Customer IHC-Fr Human 06/18/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: HaCaT-ras A5-RT3 (SCC) tumor sections
• Species: Human
• Comments: NB600-408 has worked perfectly for staining ColI in frozen tumor samples, always reproducible results

Secondary Antibody

• Secondary Description: anti-rabbit Cy-2 antibody (Dianova), host species: donkey, dilution 1:100 in 12% BSA

Details

• Detection Notes: IF Microscope
• Fixation Details: Fixation:5 min 80% Methanol @ RT, 2 min Aceton @ -20C.
• Wash Description: 1x dH2O, 3x 10 min PBS, 1x dH2O

Comments

• Comments: NB600-408 has worked perfectly for staining ColI in frozen tumor samples, always reproducible results

reviewed by: Nathan Weidenhamer ICC Human 02/04/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: human dermal fibroblast derived cell sheet
• Species: Human

Blocking

• Blocking Details: 5% normal donkey serum, 1 hr at room temp.

Primary Anitbody

• Dilution Ratio: 5 ug/ml in 5% normal donkey serum, overnight at 4 degrees C

Secondary Antibody

• Secondary Description: donkey anti-rabbit Cy3
• Secondary Manufacturer Cat#: Jackson ImmunoResearch, 711-165-152
• Secondary Concentration: 4 ug/ml in PBS

Details

• Fixation Details: 4% PFA, 1 hr at room temp.
• Wash Description: 3 x 10 min, PBS

reviewed by: Verified Customer WB Human 09/04/2012

Summary

• Application: Western Blot
• Sample Tested: Fibroblast Cell Media
• Species: Human
• Comments: The antibody works well and seems to detect both N and C cleaved collagen 1 fragments (see blot) as well as the mature collagen form. Western blot was done under denaturing conditions so not restricted to non-denaturing gels. Antibody could be diluted to higher than 1:1000.

Blocking

• Blocking Details: 5% Milk in PBS-T, 20min, RT

Primary Anitbody

• Dilution Ratio: 1:1000 in 5% Milk in PBS-T, O/N, 4C

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG-HRP
• Secondary Concentration: 1:5000

Details

• Detection Notes: ECL Prime, 10 seconds

Comments

• Comments: The antibody works well and seems to detect both N and C cleaved collagen 1 fragments (see blot) as well as the mature collagen form. Western blot was done under denaturing conditions so not restricted to non-denaturing gels. Antibody could be diluted to higher than 1:1000.

FAQs for Collagen I Antibody (NB600-408). (Showing 1 - 2 of 2 FAQs).

1. Could you please advise if this antibody works for IHC-P application using bone tissues?
   • NB600-408 has been tested and validated for use in the following applications and species: Western blot, ELISA, IP and IHC-Paraffin in human, mouse, rat and bovine. We have not specifically tested bone tissue.
2. Do you follow standard Western procedures with this collagen Ab or can the protein not be denatured?
   • Since we have not posted any special instruction for using this antibody in WB on our web, you could use the standard WB protocol. Although this collagen I a 1 is a huge protein (Uniprot site), the structure is relatively simple; with few modified amino acids and glycosylations. The detection of this protein with SDS-PAGE (denaturing gel) will ensure the molecular size of the protein could be accurately detected and confirmed, even though the glycosylations may alter the true MW. The manufacture of NB600-408 was carried out by the injections of rabbit with the immunogens derived from native collagen Type I fragments derived from human and bovine placenta after the limited pepsin digestions and selective salt precipitations. Therefore this antibody should recognize the native, undenatured collagen 1 alpha 1. If using the undenatured lysates in WB, you should expect to detect a very large protein complexes on your gel/WB, as the collagen I a 1 interacts with itself and/or other components in the extracellular matrices; but our WB protocol should be able to serve the propose.

Bioinformatics

• Gene Symbol: COL1A1
• Entrez:
  • 282187(Bovine)
  • 1277(Human)
  • 29393(Rat)
• Uniprot:
  • P02452()
  • P02453(Bovine)