Clusterin/APOJ Antibody (CLI-9) - BSA Free

Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: CLI-9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Clusterin/APOJ Antibody (CLI-9) - BSA Free Summary

• Immunogen: Purified human clusterin (TRPM-2).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLU
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Clusterin/APOJ Antibody (CLI-9) - BSA Free

• 40
• 40, sulfated glycoprotein 2
• Aging-associated gene 4 protein
• aging-associated protein 4
• APOJ
• apo-J
• Apolipoprotein J
• CLI
• CLIclusterin (complement lysis inhibitor, SP-40
• CLU
• Clusterin
• Complement cytolysis inhibitor
• complement lysis inhibitor
• Complement-associated protein SP-40
• Ku70-binding protein 1
• KUB1SGP2
• MGC24903
• NA1/NA2
• SGP-2
• SP-40
• sulfated glycoprotein 2
• Testosterone-repressed prostate message 2
• testosterone-repressed prostate message 2, apolipoprotein J)
• TRPM-2
• TRPM-2TRPM2

Background

APOJ / Clusterin Antibody (CLI-9) (SGP-2/sulfated glycoprotein-2; CLI; Apolipoprotein J (Apo J); TRPM-2) is the first identified extracellular mammalian chaperone and appears to be implicated in such diverse processes as sperm maturation, regulation of complement activation, programmed cell death, tissue remodelling and lipid transport. Increases in clusterin mRNA and protein have been consistently detected in apoptotic cell death paradigms, establishing clusterin gene expression as a prominent marker of apoptotic cell loss. A dual role has been shown for clusterin in that cells can be protected from damage and dead cells can be more easily removed after some types of cellular damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Clusterin/APOJ Antibody (NBP1-97521)(2)

Publications using NBP1-97521

• T Chen et al. Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21 dependent but p53 independent. Cancer Res. 64, 7412. 2004-01-01 [PMID: 15492264]
• C D Hough et al. Large-scale serial analysis of gene expression reveals genes differentially expressed in ovarian cancer . Cancer Res. 60, 6281. 2000-01-01 [PMID: 11103784]

Bioinformatics

• Gene Symbol: CLU