PICALM Antibody

Images

 
Western Blot: PICALM Antibody [NBP1-86658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human duodenum shows strong positivity in glandular cells.
Western Blot: PICALM Antibody [NBP1-86658] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Orthogonal Strategies: Western Blot: PICALM Antibody [NBP1-86658] - Analysis in human cell lines Caco-2 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

PICALM Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRL
Localization
Membrane, clathrin-coated pit, Golgi apparatus. Cytomplasim vesicle, clathrin-coated vesicle.
Specificity
Specificity of human, mouse, rat PICALM antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PICALM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended
Positive Control
PICALM Lysate (NBP2-66350)
Control Peptide
PICALM Protein (NBP1-86658PEP)
Publications
Read Publications using
NBP1-86658 in the following applications:

  • IHC
    1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26005850).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PICALM Antibody

  • CALMclathrin assembly lymphoid-myeloid leukemia
  • Clathrin assembly lymphoid myeloid leukemia protein
  • CLTHphosphatidylinositol-binding clathrin assembly protein
  • LAP
  • phosphatidylinositol binding clathrin assembly protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PICALM Antibody (NBP1-86658)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for PICALM Antibody (NBP1-86658)

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Diseases for PICALM Antibody (NBP1-86658)

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Bioinformatics

Gene Symbol PICALM
Entrez