PICALM Antibody

Images

 
Western Blot: PICALM Antibody [NBP1-86658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining in human placenta and skeletal muscle tissues using NBP1-86658 antibody. Corresponding PICALM RNA-seq data are ...read more
Orthogonal Strategies: Western Blot: PICALM Antibody [NBP1-86658] - Analysis in human cell lines Caco-2 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Independent Antibodies: Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum, duodenum, placenta and skeletal muscle using Anti-PICALM antibody NBP1-86658 (A) shows similar ...read more
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PICALM Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRL
Localization
Membrane, clathrin-coated pit, Golgi apparatus. Cytomplasim vesicle, clathrin-coated vesicle.
Specificity
Specificity of human PICALM antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PICALM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
WB reported in scientific literature (PMID: 27350344). For IHC-Paraffin HIER pH6 retrieval is recommended
Control
PICALM Knockout HeLa Cell Lysate
Control Peptide
PICALM Protein (NBP1-86658PEP)
Publications
Read Publications using
NBP1-86658 in the following applications:

  • IHC
    1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26005850).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PICALM Antibody

  • CALMclathrin assembly lymphoid-myeloid leukemia
  • Clathrin assembly lymphoid myeloid leukemia protein
  • CLTHphosphatidylinositol-binding clathrin assembly protein
  • LAP
  • phosphatidylinositol binding clathrin assembly protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PICALM Antibody (NBP1-86658)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PICALM Antibody (NBP1-86658) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for PICALM Antibody (NBP1-86658)

Discover related pathways, diseases and genes to PICALM Antibody (NBP1-86658). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol PICALM
Entrez