PICALM Antibody

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining in human placenta and skeletal muscle tissues using NBP1-86658 antibody. Corresponding PICALM RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum, duodenum, placenta and skeletal muscle using Anti-PICALM antibody NBP1-86658 (A) shows similar protein distribution across tissues to independent antibody NBP1-86659 (B).

Western Blot: PICALM Antibody [NBP1-86658] - Analysis in human cell lines Caco-2 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Western Blot: PICALM Antibody [NBP1-86658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PICALM Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRL
• Localization: Membrane, clathrin-coated pit, Golgi apparatus. Cytomplasim vesicle, clathrin-coated vesicle.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PICALM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 27350344). For IHC-Paraffin HIER pH6 retrieval is recommended
• Control Peptide:

  PICALM Protein (NBP1-86658PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26005850).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PICALM Antibody

• CALMclathrin assembly lymphoid-myeloid leukemia
• Clathrin assembly lymphoid myeloid leukemia protein
• CLTHphosphatidylinositol-binding clathrin assembly protein
• LAP
• phosphatidylinositol binding clathrin assembly protein

Background

Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PICALM Antibody (NBP1-86658)(6)

Publications using NBP1-86658

• Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP et al. Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity Science advances 2022-05-27 [PMID: 35613266]
• Thomas RS, Henson A, Gerrish A et al. Decreasing the expression of PICALM reduces endocytosis and the activity of beta-secretase: implications for Alzheimer's disease. BMC Neurosci 2016-07-18 [PMID: 27430330] (WB)
• Tsai-Teng T, Chin-Chu C, Li-Ya L et al. Erinacine A-enriched Hericium erinaceus mycelium ameliorates Alzheimer's disease-related pathologies in APPswe/PS1dE9 transgenic mice J. Biomed. Sci. 2016-06-28 [PMID: 27350344] (WB, Human)
• Mercer JL, Argus JP, Crabtree DM et al. Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis. PLoS One 2015-01-01 [PMID: 26075887] (WB, Human)
• Zhen Zhao, Abhay P Sagare, Qingyi Ma et al. Central role for PICALM in amyloid--beta blood-brain barrier transcytosis and clearance. Nature Neuroscience 2015-05-25 [PMID: 26005850] (WB, IF/IHC, Mouse)
• Armstrong A, Mattsson N, Appelqvist H et al. Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer's Disease. Neuromolecular Med. 2013-10-08 [PMID: 24101586] (WB, Human)

Bioinformatics

• Gene Symbol: PICALM
• Entrez:
  • 8301(Human)