PICALM Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining in human placenta and skeletal muscle tissues using NBP1-86658 antibody. Corresponding PICALM RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum, duodenum, placenta and skeletal muscle using Anti-PICALM antibody NBP1-86658 (A) shows similar ...read more
Orthogonal Strategies: Western Blot: PICALM Antibody [NBP1-86658] - Analysis in human cell lines Caco-2 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western Blot: PICALM Antibody [NBP1-86658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PICALM Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRL
Localization
Membrane, clathrin-coated pit, Golgi apparatus. Cytomplasim vesicle, clathrin-coated vesicle.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PICALM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 27350344). For IHC-Paraffin HIER pH6 retrieval is recommended
Control Peptide
PICALM Protein (NBP1-86658PEP)
Publications
Read Publications using
NBP1-86658 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26005850).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PICALM Antibody

  • CALMclathrin assembly lymphoid-myeloid leukemia
  • Clathrin assembly lymphoid myeloid leukemia protein
  • CLTHphosphatidylinositol-binding clathrin assembly protein
  • LAP
  • phosphatidylinositol binding clathrin assembly protein

Background

Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PICALM Antibody (NBP1-86658)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IF/IHC, WB.


Filter By Application
IF/IHC
(1)
WB
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-86658 Applications Species
Zhang Z, Yu K, You Y et al. Comprehensive characterization of human brain-derived extracellular vesicles using multiple isolation methods: Implications for diagnostic and therapeutic applications Journal of Extracellular Vesicles 2023-08-10 [PMID: 37563857] (WB) WB
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP et al. Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity Science advances 2022-05-27 [PMID: 35613266]
Thomas RS, Henson A, Gerrish A et al. Decreasing the expression of PICALM reduces endocytosis and the activity of beta-secretase: implications for Alzheimer's disease. BMC Neurosci 2016-07-18 [PMID: 27430330] (WB) WB
Tsai-Teng T, Chin-Chu C, Li-Ya L et al. Erinacine A-enriched Hericium erinaceus mycelium ameliorates Alzheimer's disease-related pathologies in APPswe/PS1dE9 transgenic mice J. Biomed. Sci. 2016-06-28 [PMID: 27350344] (WB, Human) WB Human
Mercer JL, Argus JP, Crabtree DM et al. Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis. PLoS One 2015-01-01 [PMID: 26075887] (WB, Human) WB Human
Zhen Zhao, Abhay P Sagare, Qingyi Ma et al. Central role for PICALM in amyloid--beta blood-brain barrier transcytosis and clearance. Nature Neuroscience 2015-05-25 [PMID: 26005850] (WB, IF/IHC, Mouse) WB, IF/IHC Mouse
Armstrong A, Mattsson N, Appelqvist H et al. Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer's Disease. Neuromolecular Med. 2013-10-08 [PMID: 24101586] (WB, Human) WB Human

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PICALM
Entrez