LRP2 Antibody (CD7D5)

Images

 
Immunohistochemistry: LRP2 Antibody (CD7D5) [NB110-96417] - Representative immunofluorescence stainings of control (Tead4m-z+) and Tead4 maternal/zygotic mutant (Tead4m-z-) embryos for Krt8 and Krt18 (16 cell stage ...read more
Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule.
Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Immunofluorescence staining of Megalin in the brush border of the proximal tubule.
Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were ...read more
Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining on human kidney at 1:100, overnight incubation at 4C. Image from a review by a confirmed customer.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clone
CD7D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

LRP2 Antibody (CD7D5) Summary

Immunogen
Purified human LRP2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LRP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:200
Theoretical MW
600 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB110-96417 in the following applications:

Publications
Read Publications using
NB110-96417 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for LRP2 Antibody (CD7D5)

  • calcium sensor protein
  • DBS
  • DBSlow-density lipoprotein receptor-related protein 2
  • EC 1.1.2.3
  • EC 3.4.21.9
  • Glycoprotein 330
  • gp330
  • Heymann nephritis antigen homolog
  • low density lipoprotein receptor-related protein 2
  • LRP2
  • LRP-2
  • Megalin

Background

LRP2, an approx. 600 kDa transmembrane glycoprotein is a large cell surface receptor that mediates the binding and internalization of a number of structurally and functionally distinct ligands from the lipoprotein and protease inhibitor families.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRP2 Antibody (NB110-96417)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, PLA.


Filter By Application
ICC/IF
(4)
IHC
(5)
IHC-Fr
(1)
IHC-P
(4)
PLA
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(8)
Rat
(3)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB110-96417 Applications Species
Sander V, Przepiorski A, Crunk AE et al. Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells STAR protocols Dec 18 2020 [PMID: 33377044] (IHC, Human) IHC Human
Seo JA, Kang MC, Yang WM et al. Apolipoprotein J is a hepatokine regulating muscle glucose metabolism and insulin sensitivity Nat Commun Apr 24 2020 [PMID: 32332780] (PLA, Mouse) PLA Mouse
Suzuki C, Tanida I, Oliva Trejo JA, et al. Autophagy Deficiency in Renal Proximal Tubular Cells Leads to an Increase in Cellular Injury and Apoptosis under Normal Fed Conditions Int J Mol Sci Dec 25 2019 [PMID: 31881660] (ICC/IF, IHC, Mouse) ICC/IF, IHC Mouse
Fels J, Scharner B, Zarbock R, Zavala Guevara IP et al. Cadmium Complexed with beta 2-Microglubulin, Albumin and Lipocalin-2 rather than Metallothionein Cause Megalin:Cubilin Dependent Toxicity of the Renal Proximal Tubule Int J Mol Sci May 14 2019 [PMID: 31091675] (ICC/IF, Rat) ICC/IF Rat
Kinguchi, S;Wakui, H;Azushima, K;Haruhara, K;Koguchi, T;Ohki, K;Uneda, K;Matsuda, M;Haku, S;Yamaji, T;Yamada, T;Kobayashi, R;Minegishi, S;Ishigami, T;Yamashita, A;Fujikawa, T;Tamura, K; Effects of ATRAP in Renal Proximal Tubules on Angiotensin-Dependent Hypertension J Am Heart Assoc Apr 16 2019 [PMID: 30977419] (IHC, Mouse) IHC Mouse
Mohr J, Voggel J, Vohlen C et al. IL-6/Smad2 signaling mediates acute kidney injury and regeneration in a murine model of neonatal hyperoxia FASEB J. Feb 5 2019 [PMID: 30721632] (IHC-P, Mouse) IHC-P Mouse
Schuh CD, Polesel M, Platonova E et al. Combined Structural and Functional Imaging of the kidney Reveals Major Axial Differences in Proximal Tubule Endocytosis. J. Am. Soc. Nephrol. Oct 9 2018 [PMID: 30301861] (IHC, Mouse) IHC Mouse
Przepiorski A, Sander V, Tran T et al. A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells Stem Cell Reports Aug 14 2018 [PMID: 30033089] (IHC-P, Human) IHC-P Human
Kawano H, Ito Y, Kanai F et al. Aberrant differentiation of Tsc2-deficient teratomas associated with activation of the mTORC1-TFE3 pathway. Oncol. Rep. 2015 Nov 01 [PMID: 26352760]
Ohsawa M, Tamura K, Wakui H et al. Deletion of the angiotensin II type 1 receptor-associated protein enhances renal sodium reabsorption and exacerbates angiotensin II-mediated hypertension. Kidney Int. 2014 Apr 02 [PMID: 24694992] (IHC-P, Mouse) IHC-P Mouse
Show All 15 Publications.

Review for LRP2 Antibody (NB110-96417) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-96417:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin LRP2 NB110-96417
Enlarge
4
reviewed by:
Anonymous
IHC-P Human 10/24/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney
SpeciesHuman
Lot0903R02-2

Blocking

Blocking Details3ï¼…H2O2, 15min

Primary Anitbody

Dilution RatioDilution rate 1:100, incubation time 24h at 4c.

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG, MAX-PO
Secondary Manufacturer Cat#424132

Details

Detection NotesDAB 15min
Fixation DetailsFormalin fixed, microwave heat antigen retrieval, using citrate buffers (pH6.0)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for LRP2 Antibody (NB110-96417). (Showing 1 - 1 of 1 FAQs).

  1. Could you please forward me the above reference or an IHC protocol for this product?
    • A: Unfortunately we do not have a product specific protocol for this particular antibody. The recommended dilution range for IHC staining is indicated on the datasheet to be between 1:10-1:2000, and should be optimized based on how highly expressed the protein is in your samples. Our standard IHC protocol will also serve as a useful guide to running your assay. Please see these links to product specific references PubMed 21896500 and PubMed 23262319 for additional protocol details.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-96417AF350
Alexa Fluor 405 NB110-96417AF405
Alexa Fluor 488 NB110-96417AF488
Alexa Fluor 532 NB110-96417AF532
Alexa Fluor 594 NB110-96417AF594
Alexa Fluor 647 NB110-96417AF647
Alexa Fluor 700 NB110-96417AF700
Alexa Fluor 750 NB110-96417AF750
Biotin NB110-96417B
DyLight 350 NB110-96417UV
DyLight 405 NB110-96417V
DyLight 488 NB110-96417G
DyLight 550 NB110-96417R
DyLight 594 NB110-96417DL594
DyLight 650 NB110-96417C
DyLight 680 NB110-96417FR
DyLight 755 NB110-96417IR
FITC NB110-96417F
HRP NB110-96417H
Janelia Fluor 549 NB110-96417JF549
Janelia Fluor 646 NB110-96417JF646

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Recent Reviews

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5
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4
1
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2
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1
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Anonymous
10/24/2013
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol LRP2
Entrez
Uniprot