Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were ...read more
Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule.
Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Immunofluorescence staining of Megalin in the brush border of the proximal tubule.
Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining on human kidney at 1:100, overnight incubation at 4C. Image from a review by a confirmed customer.
600 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NB110-96417 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Protein G purified
Alternate Names for LRP2 Antibody (CD7D5)
calcium sensor protein
DBSlow-density lipoprotein receptor-related protein 2
Heymann nephritis antigen homolog
low density lipoprotein receptor-related protein 2
Megalin/gp330, an approx. 600 kDa transmembrane glycoprotein is a large cell surface receptor that mediates the binding and internalization of a number of structurally and functionally distinct ligands from the lipoprotein and protease inhibitor families.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for LRP2 Antibody (NB110-96417). (Showing 1 - 1 of 1 FAQs).
Could you please forward me the above reference or an IHC protocol for this product?
A: Unfortunately we do not have a product specific protocol for this particular antibody. The recommended dilution range for IHC staining is indicated on the datasheet to be between 1:10-1:2000, and should be optimized based on how highly expressed the protein is in your samples. Our standard IHC protocol will also serve as a useful guide to running your assay. Please see these links to product specific references PubMed 21896500 and PubMed 23262319 for additional protocol details.
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