LRP2 Antibody (CD7D5) - Azide and BSA Free

Images

 
Immunohistochemistry: LRP2 Antibody (CD7D5) [NB110-96417] - Representative immunofluorescence stainings of control (Tead4m-z+) and Tead4 maternal/zygotic mutant (Tead4m-z-) embryos for Krt8 and Krt18 (16 cell stage ...read more
Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule.
Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Immunofluorescence staining of Megalin in the brush border of the proximal tubule.
Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of LRP2 [CD7D5] antibody. The staining was developed using HRP labeled ...read more
Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were ...read more
Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining on human kidney at 1:100, overnight incubation at 4C. Image from a review by a confirmed customer.
Impairment of autophagy in Atg7-deficient renal proximal tubular cells. (B) The massive accumulation of p62 in kidneys of 2-month old Atg7flox/flox;KAP-Cre+ mouse. The cortico-medullary region of each kidney in 2 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
CD7D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

LRP2 Antibody (CD7D5) - Azide and BSA Free Summary

Immunogen
Purified human LRP2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LRP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:200
Theoretical MW
600 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB110-96417 in the following applications:

Publications
Read Publications using
NB110-96417 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for LRP2 Antibody (CD7D5) - Azide and BSA Free

  • calcium sensor protein
  • DBS
  • DBSlow-density lipoprotein receptor-related protein 2
  • EC 1.1.2.3
  • EC 3.4.21.9
  • Glycoprotein 330
  • gp330
  • Heymann nephritis antigen homolog
  • low density lipoprotein receptor-related protein 2
  • LRP2
  • LRP-2
  • Megalin

Background

LRP2, an approx. 600 kDa transmembrane glycoprotein is a large cell surface receptor that mediates the binding and internalization of a number of structurally and functionally distinct ligands from the lipoprotein and protease inhibitor families.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRP2 Antibody (NB110-96417)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, IHC-WhMt, PLA.


Filter By Application
ICC/IF
(5)
IF/IHC
(6)
IHC-Fr
(1)
IHC-P
(5)
IHC-WhMt
(1)
PLA
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(9)
Rat
(3)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB110-96417 Applications Species
Lian E, Pietrobon A, Stanford WL Differentiation and single-cell RNA-seq analyses of human pluripotent-stem-cell-derived renal organoids STAR protocols 2023-05-22 [PMID: 37220001] (ICC/IF, Human) ICC/IF Human
Polesel M, Kaminska M, Haenni D et al. Spatiotemporal organisation of protein processing in the kidney Nature communications 2022-09-29 [PMID: 36175561] (IF/IHC, Mouse) IF/IHC Mouse
Pietrobon A, Yockell-Lelievre J, Flood TA, Stanford WL Renal organoid modeling of tuberous sclerosis complex reveals lesion features arise from diverse developmental processes Cell reports 2022-07-05 [PMID: 35793620] (IHC-WhMt, Human)

Details:
Sample type: Renal organoids
IHC-WhMt Human
Sander V, Przepiorski A, Crunk AE et al. Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells STAR protocols 2020-12-18 [PMID: 33377044] (IF/IHC, Human) IF/IHC Human
Seo JA, Kang MC, Yang WM et al. Apolipoprotein J is a hepatokine regulating muscle glucose metabolism and insulin sensitivity Nat Commun 2020-04-24 [PMID: 32332780] (PLA, Mouse) PLA Mouse
Suzuki C, Tanida I, Oliva Trejo JA, et al. Autophagy Deficiency in Renal Proximal Tubular Cells Leads to an Increase in Cellular Injury and Apoptosis under Normal Fed Conditions Int J Mol Sci 2019-12-25 [PMID: 31881660] (IF/IHC, ICC/IF, Mouse) IF/IHC, ICC/IF Mouse
Fels J, Scharner B, Zarbock R, Zavala Guevara IP et al. Cadmium Complexed with beta 2-Microglubulin, Albumin and Lipocalin-2 rather than Metallothionein Cause Megalin:Cubilin Dependent Toxicity of the Renal Proximal Tubule Int J Mol Sci 2019-05-14 [PMID: 31091675] (ICC/IF, Rat) ICC/IF Rat
Kinguchi, S;Wakui, H;Azushima, K;Haruhara, K;Koguchi, T;Ohki, K;Uneda, K;Matsuda, M;Haku, S;Yamaji, T;Yamada, T;Kobayashi, R;Minegishi, S;Ishigami, T;Yamashita, A;Fujikawa, T;Tamura, K; Effects of ATRAP in Renal Proximal Tubules on Angiotensin-Dependent Hypertension J Am Heart Assoc 2019-04-16 [PMID: 30977419] (IF/IHC, Mouse) IF/IHC Mouse
Mohr J, Voggel J, Vohlen C et al. IL-6/Smad2 signaling mediates acute kidney injury and regeneration in a murine model of neonatal hyperoxia FASEB J. 2019-02-05 [PMID: 30721632] (IHC-P, Mouse) IHC-P Mouse
Schuh CD, Polesel M, Platonova E et al. Combined Structural and Functional Imaging of the kidney Reveals Major Axial Differences in Proximal Tubule Endocytosis. J. Am. Soc. Nephrol. 2018-10-09 [PMID: 30301861] (IF/IHC, Mouse) IF/IHC Mouse
Show All 18 Publications.

Review for LRP2 Antibody (NB110-96417) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-96417:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin LRP2 NB110-96417
Enlarge
4
reviewed by:
Verified Customer
IHC-P Human 10/24/2013
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney
SpeciesHuman
Lot0903R02-2

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for LRP2 Antibody (NB110-96417). (Showing 1 - 1 of 1 FAQs).

  1. Could you please forward me the above reference or an IHC protocol for this product?
    • A: Unfortunately we do not have a product specific protocol for this particular antibody. The recommended dilution range for IHC staining is indicated on the datasheet to be between 1:10-1:2000, and should be optimized based on how highly expressed the protein is in your samples. Our standard IHC protocol will also serve as a useful guide to running your assay. Please see these links to product specific references PubMed 21896500 (https://pubmed.ncbi.nlm.nih.gov/21896500/) and PubMed 23262319 (https://pubmed.ncbi.nlm.nih.gov/23262319/) for additional protocol details.

Secondary Antibodies

 

Isotype Controls

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Blogs on LRP2

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Recent Reviews

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5
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Verified Customer
10/24/2013
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol LRP2
Entrez
Uniprot