Clusterin/APOJ: Novus Biologicals

AF2747

Clusterin/APOJ Antibody [Unconjugated]

100 μg

AF2747

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Clusterin was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Clusterin was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Clusterin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>Conservation of clusterin‐dependent alpha ‐synuclein preformed fibrils (pffs) uptake from mouse to human astrocytes. (a) Representative image of hiAstrocytes (27 days of differentiation) treated with * alpha ‐synuclein pffs for 48 hr. Scale bar 10 μm. (b) Cell lysates from hiAstrocytes (27 days of differentiation) treated with alpha ‐synuclein pffs, or monomeric alpha ‐synuclein (m), for 48 hr were subjected to immunoblotting using alpha ‐synuclein and GAPDH antibodies. Asterisks in the alpha ‐synuclein immunoblot indicate alpha ‐synuclein nonspecific bands. (c) Medium from hiAstrocytes (27 days of differentiation) treated with alpha ‐synuclein pffs, or monomeric alpha ‐synuclein (M), for 48 hr were subjected to immunoblotting using clusterin antibody. Ponceau‐S staining was used as loading control. (d) Quantification of clusterin is normalized to GAPDH protein of cell lysates. Data are representative of three independent experiments and are expressed as the mean ± SD. Data were analyzed using unpaired t test; *p = .0446. (e) Cell lysates from hiAstrocytes (31 days of differentiation) transfected with negative control (NC) or clusterin siRNA for 24 hr were subjected to immunoblotting using clusterin and GAPDH antibodies. Asterisk in the clusterin immunoblot indicates clusterin nonspecific band. (f) Quantification of clusterin precursor is normalized to GAPDH. Data are representative of three independent experiments and are expressed as the mean ± SD. Data were analyzed using unpaired t test; *p = .0165. (g) Representative images of hiAstrocytes (35 days of differentiation) transfected with NC or clusterin siRNA and treated with * alpha ‐synuclein pffs for 24 hr. Scale bar 10 μm. (h) Quantification of * alpha ‐synuclein pffs is shown as mean of fluorescence intensity from three independent experiments (~50 cells per experiment). Quantification of * alpha ‐synuclein pffs is calculated as fluorescence intensity divided by the cell area (μm2). Data are expressed as the mean ± SD. Data were analyzed using unpaired t test; *p = .0178.

<b>Genetic Strategies Validation. </b>Extracellular clusterin limits the uptake of alpha ‐synuclein preformed fibrils (pffs) by murine primary astrocytes. (a) Cell lysates from clusterin wild‐type (WT) and knock‐out (KO) astrocytes were subjected to immunoblotting using clusterin and GAPDH antibodies. Asterisk indicates clusterin precursor. (b) Maximum intensity Z‐projection confocal images of clusterin WT and KO astrocytes treated with * alpha ‐synuclein pffs for 4 hr. Scale bar 5 μm. (c) Quantification of * alpha ‐synuclein pffs is shown as mean of fluorescence intensity from six independent experiments (~50 cells per experiment). Quantification of * alpha ‐synuclein pffs is calculated as fluorescence intensity divided by the cell area (μm2). Data are expressed as the mean ± SD. Data were analyzed using unpaired t test; *p = .0315. (d) Cell lysates from clusterin WT and KO primary astrocytes treated with pffs for 16 hr were subjected to immunoblotting using alpha ‐synuclein and GAPDH antibodies. (e) Quantification of intracellular alpha ‐synuclein is normalized to GAPDH protein. Data are representative of eight independent experiments and are expressed as the mean ± SD. Data were analyzed using unpaired t test; *p = .0239. (f) Cell lysates from clusterin KO primary astrocytes treated with pffs for 16 hr in a medium containing or not containing extracellular clusterin were subjected to immunoblotting using alpha ‐synuclein, clusterin and GAPDH antibodies. (g) Quantification of intracellular alpha ‐synuclein is normalized to GAPDH protein. Data are representative of four independent experiments and are expressed as the mean ± SD. Data were analyzed using unpaired t test; *p = .0134. (h) Cell lysates from clusterin WT and KO primary astrocytes treated with pffs and chloroquine (CQ) for 16 hr were subjected to immunoblotting using alpha ‐synuclein and GAPDH antibodies. (i) Quantification of intracellular alpha ‐synuclein normalized to GAPDH protein. Data are representative of six independent experiments and are expressed as the mean ± SD. Data were analyzed

Clusterin protein levels are increased in two different animal models of Parkinson's disease. (a) Lysates from contralateral and ipsilateral ventral midbrain of mice injected with adeno‐associated virus (AAV)‐h alpha ‐synuclein and sacrificed after 8 weeks were subjected to immunoblotting using clusterin and GAPDH antibodies. (b) Quantification of total cleaved clusterin is normalized to GAPDH protein. Data are representative of five animals and are expressed as the mean ± SD. Data were analyzed using one‐sample t test; *p = .0253. (c) Representative images of clusterin‐GFAP double labeling in the ipsilateral and contralateral striatum of mice injected with AAV‐h alpha ‐synuclein and sacrificed after 8 weeks. Scale bar 20 μm. (d) Representative images of clusterin‐S100 beta double labeling in the substantia nigra of mice injected with alpha ‐synuclein preformed fibrils (pffs) and sacrificed after 4 weeks. Scale bar 20 μm. (e) Representative images of clusterin‐S100 beta double labeling in the striatum of mice injected with alpha ‐synuclein pffs and sacrificed after 4 weeks. Scale bar 20 μm [Color figure can be viewed at wileyonlinelibrary.com] Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33045109), licensed under a CC-BY license. Not internally tested by R&D Systems.

2747-HS) was serially diluted and captured by Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse Clusterin (Catalog # <a class=

2747-HS) was serially diluted and captured by Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2747-HS) was serially diluted and captured by Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

2747-HS) was serially diluted and captured by Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2747U-100UG
Alexa Fluor 405	AF2747V-100UG
Alexa Fluor 488	AF2747G-100UG
Alexa Fluor 532	AF2747X-100UG
Alexa Fluor 594	AF2747T-100UG
Alexa Fluor 647	AF2747R-100UG
Alexa Fluor 700	AF2747N-100UG
Alexa Fluor 750	AF2747S-100UG
Alexa Fluor Plus 405	AF2747AFP405-100UG
Alexa Fluor Plus 488	AF2747AFP488-100UG
Alexa Fluor Plus 555	AF2747AFP555-100UG
Alexa Fluor Plus 594	AF2747AFP594-100UG
Alexa Fluor Plus 647	AF2747AFP647-100UG
Alexa Fluor Plus 680	AF2747AFP680-100UG
Allophycocyanin	AF2747A
Biotin	BAF2747
CoraFluor 1	AF2747CL1
DyLight 350	AF2747D
DyLight 405	AF2747E
DyLight 488	AF2747K
DyLight 550	AF2747L
DyLight 594	AF2747M
DyLight 650	AF2747W
DyLight 680	AF2747Y
DyLight 755	AF2747Z
FITC	AF2747F
HRP	AF2747H
Janelia Fluor 525	AF2747JF525
Janelia Fluor 549	AF2747I
Janelia Fluor 646	AF2747J
Janelia Fluor 669	AF2747JF669
PE	AF2747P
PerCP	AF2747C
mFluor Violet 450 SE	AF2747MFV450
mFluor Violet 500 SE	AF2747MFV500
mFluor Violet 610 SE	AF2747MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2747-SP

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DCLU00

Human Clusterin Quantikine ELISA Kit

(16 Publications)

1 Kit

DCLU00

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	SCLU00
HRP	PDCLU00

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	SCLU00
Carrier Free	PDCLU00

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MAB2937

Clusterin/APOJ Antibody (350227) [Unconjugated]

(6 Publications)

100 μg

MAB2937

HAF018). Specific bands were detected for Clusterin Precursor at approximately 60-65 kDa and Clusterin a chain at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB2937) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). Specific bands were detected for Clusterin Precursor at approximately 60-65 kDa and Clusterin a chain at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB2937) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Clusterin Precursor at approximately 60-65 kDa and Clusterin a chain at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Clusterin was detected in immersion fixed frozen sections of mouse spleen using Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB2937) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Clusterin was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB2937) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

$Human CLU_45 kDa is translated from an AUG start site in Exon 3.(A) Empty vector (pCMV6), V5-tagged pCMV6-human CLU Exon 2–9, or Exon 3–9 constructs were transfected into human SH-SY5Y cells. Cell lysates were harvested and probed for CLU immunoreactivity using anti-V5 (left panel) or anti-Clusterin (right panel). (B: left panel) pCMV6-human CLU Exon 2–9 or Exon 3–9 constructs were transfected into human SH-SY5Y cells. The resulting cell lysates were treated with PNGase F or Endo H and probed for CLU immunoreactivity using anti-V5. (B: right panel) Cell lysates harvested from pCMV6-human CLU Exon 3–9-transfected SH-SY5Y cells were treated with O-glycosidase, neuraminidase, or a combination of the two and probed for expression of CLU_45 kDa protein using anti-V5. (C) pCMV6-human CLU Exon 3–9-transfected SH-SY5Y cells were subjected to cellular fractionation using the Qiagen Qproteome Mitochondrial Isolation Kit and analyzed for CLU immunoreactivity using anti-V5. Fraction isolation was confirmed using calnexin (ER), Gapdh (cytosol), lamin A/C and lamin B1 (nucleus), and VDAC and Tom20 (mitochondria). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31738162), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2937U-100UG
Alexa Fluor 405	FAB2937V-100UG
Alexa Fluor 488	FAB2937G-100UG
Alexa Fluor 532	FAB2937X-100UG
Alexa Fluor 594	FAB2937T-100UG
Alexa Fluor 647	FAB2937R-100UG
Alexa Fluor 700	FAB2937N-100UG
Alexa Fluor 750	FAB2937S-100UG
Alexa Fluor Plus 405	FAB2937AFP405-100UG
Alexa Fluor Plus 488	FAB2937AFP488-100UG
Alexa Fluor Plus 555	FAB2937AFP555-100UG
Alexa Fluor Plus 594	FAB2937AFP594-100UG
Alexa Fluor Plus 647	FAB2937AFP647-100UG
Alexa Fluor Plus 680	FAB2937AFP680-100UG
Allophycocyanin	FAB2937A
Biotin	FAB2937B
CoraFluor 1	FAB2937CL1
DyLight 350	FAB2937D
DyLight 405	FAB2937E
DyLight 488	FAB2937K
DyLight 550	FAB2937L
DyLight 594	FAB2937M
DyLight 650	FAB2937W
DyLight 680	FAB2937Y
DyLight 755	FAB2937Z
FITC	FAB2937F
HRP	FAB2937H
Janelia Fluor 525	FAB2937JF525
Janelia Fluor 549	FAB2937I
Janelia Fluor 646	FAB2937J
Janelia Fluor 669	FAB2937JF669
PE	FAB2937P
PerCP	FAB2937C
mFluor Violet 450 SE	FAB2937MFV450
mFluor Violet 500 SE	FAB2937MFV500
mFluor Violet 610 SE	FAB2937MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2937-SP

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AF2937

Clusterin/APOJ Antibody [Unconjugated]

(3 Publications)

100 μg

AF2937

HAF017). Specific bands were detected for Clusterin Precusor at approximately 60-65 kDa and Clusterin a and beta chains at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Clusterin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). Specific bands were detected for Clusterin Precusor at approximately 60-65 kDa and Clusterin a and beta chains at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Clusterin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Clusterin Precusor at approximately 60-65 kDa and Clusterin a and beta chains at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Clusterin was detected in immersion fixed paraffin-embedded of human Alzheimer's disease brain using Human Clusterin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2937) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>Extracellular clusterin interacts with alpha ‐synuclein preformed fibrils (pffs). (a) Medium from primary astrocytes treated with alpha ‐synuclein pffs for 16 hr, or with monomeric alpha ‐synuclein (m), were subjected to immunoblotting using clusterin antibody. Ponceau‐S staining was used as a loading control. (b) Quantification of clusterin is normalized to GAPDH protein of cell lysates. Data are from eight independent experiments and are expressed as the mean ± SD. Data were analyzed using unpaired t test; **p = .0028. (c) alpha ‐Synuclein pffs isolated by centrifugation from total medium of primary astrocytes treated with alpha ‐synuclein pffs, or monomeric alpha ‐synuclein (M), for 16 hr were subjected to immunoblotting using clusterin and alpha ‐synuclein antibodies. Asterisk in the alpha ‐synuclein immunoblot indicates clusterin bands still present after Clusterin antibody stripping. (d) Clusterin immunoprecipitated from the medium of primary astrocytes treated with alpha ‐synuclein pffs for 16 hr was subjected to immunoblotting using an alpha ‐synuclein antibody. (e) Quantification of clusterin— alpha ‐synuclein pffs interaction has been obtained by normalization of alpha ‐synuclein pffs bound to clusterin for alpha ‐synuclein pffs bound to protein‐G beads. Data are representative of three independent experiments and are expressed as the mean ± SD. Data were analyzed using one‐sample t test; *p = .0493. (f) Z‐stack frames of primary astrocytes treated with alpha ‐synuclein pffs for 16 hr and stained for alpha ‐synuclein (purple), clusterin (green), cadherin (red), and nuclei with DAPI (blue). Scale bar 10 μm. (g) Maximum intensity Z‐projection confocal images of primary astrocytes treated with alpha ‐synuclein pffs for 16 hr and stained for alpha ‐synuclein (purple), clusterin (red), Early Endosome Antigen 1 (EEA1) (green) and nuclei with DAPI (blue). Scale bar 10 μm. Individual points of the graphs represent each single experiment [Color figure can be viewed at wileyonlinelibrary.com] Image collected and cropped by CiteAb from the following p

$Identification of a mitochondrial CLU protein isoform.(A) DIV 9 Mitotracker-stained (green) primary neurons were probed for CLU immunoreactivity using anti-CLU H-330 (red) and visualized using 40X confocal microscopy. (B) Pure cortical mitochondria were isolated as indicated. Equal concentrations of whole tissue lysate, crude mitochondria, and pure mitochondria were analyzed via SDS-PAGE and probed for CLU immunoreactivity using anti-CLU H-330 (left panel) and anti-CLU M-18 (right panel) (n = 3 independent isolations). Biochemical characterization of isolated fractions was performed using a panel of organelle-specific antibodies: voltage-dependent anion channels (VDAC) (mitochondria), calnexin and calreticulin (ER), and Gapdh (cytosol). (C) Crude and pure mitochondria were isolated and subjected to endoglycosidase treatment using PNGase F and Endo H. Deglycosylated mitochondrial lysates were then analyzed for CLU immunoreactivity using anti-CLU M-18. Red font: deglycosylated protein isoforms; blue font: isoforms that were unaffected by glycosidase treatment; green asterisk: excess Endo H enzyme.Positive controls for deglycosylation studies.RNase B, a high mannose glycoprotein, has a single N-linked glycosylation site and was used as a positive control for endoglycosidases that cleave N-linked carbohydrates. Fetuin, a glycoprotein containing sialylated N-linked and O-linked glycans, was used as a positive control for endoglycosidases that cleave both N-linked and O-linked carbohydrates. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31738162), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2937U-100UG
Alexa Fluor 405	AF2937V-100UG
Alexa Fluor 488	AF2937G-100UG
Alexa Fluor 532	AF2937X-100UG
Alexa Fluor 594	AF2937T-100UG
Alexa Fluor 647	AF2937R-100UG
Alexa Fluor 700	AF2937N-100UG
Alexa Fluor 750	AF2937S-100UG
Alexa Fluor Plus 405	AF2937AFP405-100UG
Alexa Fluor Plus 488	AF2937AFP488-100UG
Alexa Fluor Plus 555	AF2937AFP555-100UG
Alexa Fluor Plus 594	AF2937AFP594-100UG
Alexa Fluor Plus 647	AF2937AFP647-100UG
Alexa Fluor Plus 680	AF2937AFP680-100UG
Allophycocyanin	AF2937A
Biotin	BAF2937
CoraFluor 1	AF2937CL1
DyLight 350	AF2937D
DyLight 405	AF2937E
DyLight 488	AF2937K
DyLight 550	AF2937L
DyLight 594	AF2937M
DyLight 650	AF2937W
DyLight 680	AF2937Y
DyLight 755	AF2937Z
FITC	AF2937F
HRP	AF2937H
Janelia Fluor 525	AF2937JF525
Janelia Fluor 549	AF2937I
Janelia Fluor 646	AF2937J
Janelia Fluor 669	AF2937JF669
PE	AF2937P
PerCP	AF2937C
mFluor Violet 450 SE	AF2937MFV450
mFluor Violet 500 SE	AF2937MFV500
mFluor Violet 610 SE	AF2937MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2937-SP

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2937-HS

Recombinant Human Clusterin Protein, CF

(9 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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DY5874

Human Clusterin DuoSet ELISA, 15 Plate

(6 Publications)

1 Kit (for 15 Plates)

DY5874

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MCLU00

Mouse Clusterin Quantikine ELISA Kit

(5 Publications)

1 Kit

MCLU00

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP1-06027

Clusterin/APOJ Antibody

(5 Publications)

0.1 mg

NBP1-06027

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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MAB29372

Clusterin/APOJ Antibody (350270) [Unconjugated]

(1 Publication)

100 μg

MAB29372

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Clusterin was detected in immersion fixed frozen sections of mouse spleen using Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB29372) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Clusterin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB29372) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

2937-HS) was serially diluted and captured by Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB29372) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB29373) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Clusterin (Catalog # <a class=

2937-HS) was serially diluted and captured by Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB29372) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB29373) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB29372U-100UG
Alexa Fluor 405	FAB29372V-100UG
Alexa Fluor 488	FAB29372G-100UG
Alexa Fluor 532	FAB29372X-100UG
Alexa Fluor 594	FAB29372T-100UG
Alexa Fluor 647	FAB29372R-100UG
Alexa Fluor 700	FAB29372N-100UG
Alexa Fluor 750	FAB29372S-100UG
Alexa Fluor Plus 405	FAB29372AFP405-100UG
Alexa Fluor Plus 488	FAB29372AFP488-100UG
Alexa Fluor Plus 555	FAB29372AFP555-100UG
Alexa Fluor Plus 594	FAB29372AFP594-100UG
Alexa Fluor Plus 647	FAB29372AFP647-100UG
Alexa Fluor Plus 680	FAB29372AFP680-100UG
Allophycocyanin	FAB29372A
Biotin	FAB29372B
CoraFluor 1	FAB29372CL1
DyLight 350	FAB29372D
DyLight 405	FAB29372E
DyLight 488	FAB29372K
DyLight 550	FAB29372L
DyLight 594	FAB29372M
DyLight 650	FAB29372W
DyLight 680	FAB29372Y
DyLight 755	FAB29372Z
FITC	FAB29372F
HRP	FAB29372H
Janelia Fluor 525	FAB29372JF525
Janelia Fluor 549	FAB29372I
Janelia Fluor 646	FAB29372J
Janelia Fluor 669	FAB29372JF669
PE	FAB29372P
PerCP	FAB29372C
mFluor Violet 450 SE	FAB29372MFV450
mFluor Violet 500 SE	FAB29372MFV500
mFluor Violet 610 SE	FAB29372MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB29372-SP
Carrier Free	MAB29372-500

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NBP1-90128

Clusterin/APOJ Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-90128

<b>Orthogonal Strategies Validation. </b>Staining of human cervix shows strong cytoplasmic positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.

Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.

Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-90128-25ul

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NBP1-97521

Clusterin/APOJ Antibody (CLI-9) - BSA Free

(2 Publications)

0.1 mg

NBP1-97521

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97521AF350
Alexa Fluor 405	NBP1-97521AF405
Alexa Fluor 488	NBP1-97521AF488
Alexa Fluor 532	NBP1-97521AF532
Alexa Fluor 594	NBP1-97521AF594
Alexa Fluor 647	NBP1-97521AF647
Alexa Fluor 700	NBP1-97521AF700
Alexa Fluor 750	NBP1-97521AF750
Alexa Fluor Plus 405	NBP1-97521AFP405
Alexa Fluor Plus 488	NBP1-97521AFP488
Alexa Fluor Plus 555	NBP1-97521AFP555
Alexa Fluor Plus 594	NBP1-97521AFP594
Alexa Fluor Plus 647	NBP1-97521AFP647
Alexa Fluor Plus 680	NBP1-97521AFP680
Allophycocyanin	NBP1-97521APC
Biotin	NBP1-97521B
CoraFluor 1	NBP1-97521CL1
DyLight 350	NBP1-97521UV
DyLight 405	NBP1-97521V
DyLight 488	NBP1-97521G
DyLight 550	NBP1-97521R
DyLight 594	NBP1-97521DL594
DyLight 650	NBP1-97521C
DyLight 680	NBP1-97521FR
DyLight 755	NBP1-97521IR
FITC	NBP1-97521F
HRP	NBP1-97521H
Janelia Fluor 525	NBP1-97521JF525
Janelia Fluor 549	NBP1-97521JF549
Janelia Fluor 646	NBP1-97521JF646
Janelia Fluor 669	NBP1-97521JF669
PE	NBP1-97521PE
PerCP	NBP1-97521PCP
mFluor Violet 450 SE	NBP1-97521MFV450
mFluor Violet 500 SE	NBP1-97521MFV500
mFluor Violet 610 SE	NBP1-97521MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-97521-0.025mg

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NB500-552

Clusterin/APOJ Antibody (Hs-3) - BSA Free

(1 Publication)

0.1 mg

NB500-552

Analysis of human clusterin using mouse monoclonal antibody Hs-3 on lysates of MCF-7 cell line and MOLT-4 cell line (clusterin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-clusterin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Clusterin was detected around 40 kDa.

Reactivity:

Hu, Bv(-), Ca(-), Fe(-), Po(-)

Species Glossary

Reactivity: Hu, Bv(-), Ca(-), Fe(-), Po(-)

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-552AF350
Alexa Fluor 405	NB500-552AF405
Alexa Fluor 488	NB500-552AF488
Alexa Fluor 532	NB500-552AF532
Alexa Fluor 594	NB500-552AF594
Alexa Fluor 647	NB500-552AF647
Alexa Fluor 700	NB500-552AF700
Alexa Fluor 750	NB500-552AF750
Alexa Fluor Plus 405	NB500-552AFP405
Alexa Fluor Plus 488	NB500-552AFP488
Alexa Fluor Plus 555	NB500-552AFP555
Alexa Fluor Plus 594	NB500-552AFP594
Alexa Fluor Plus 647	NB500-552AFP647
Alexa Fluor Plus 680	NB500-552AFP680
Allophycocyanin	NB500-552APC
Biotin	NB500-552B
CoraFluor 1	NB500-552CL1
DyLight 350	NB500-552UV
DyLight 405	NB500-552V
DyLight 488	NB500-552G
DyLight 550	NB500-552R
DyLight 594	NB500-552DL594
DyLight 650	NB500-552C
DyLight 680	NB500-552FR
DyLight 755	NB500-552IR
FITC	NB500-552F
HRP	NB500-552H
Janelia Fluor 525	NB500-552JF525
Janelia Fluor 549	NB500-552JF549
Janelia Fluor 646	NB500-552JF646
Janelia Fluor 669	NB500-552JF669
PE	NB500-552PE
PerCP	NB500-552PCP
mFluor Violet 450 SE	NB500-552MFV450
mFluor Violet 500 SE	NB500-552MFV500
mFluor Violet 610 SE	NB500-552MFV610

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CLU Products By Gene Name

Clusterin/APOJ Antibody [Unconjugated]

Human Clusterin Quantikine ELISA Kit

Clusterin/APOJ Antibody (350227) [Unconjugated]

Clusterin/APOJ Antibody [Unconjugated]

Recombinant Human Clusterin Protein, CF

Human Clusterin DuoSet ELISA, 15 Plate

Mouse Clusterin Quantikine ELISA Kit

Clusterin/APOJ Antibody

Clusterin/APOJ Antibody (350270) [Unconjugated]

Clusterin/APOJ Antibody - BSA Free

Clusterin/APOJ Antibody (CLI-9) - BSA Free

Clusterin/APOJ Antibody (Hs-3) - BSA Free