CD44 Antibody (CL13318) - Azide and BSA Free

Analysis in human cervix, uterine and skeletal muscle tissues using NBP3-44377 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.

Staining of human breast cancer (metastasis to ovary) shows moderate membranous positivity in tumor cells.

Staining of human colon shows strong membranous positivity in lymphoid cells.

Staining of human endometrial cancer shows strong membranous positivity in tumor cells.

Staining of human lymph node shows strong membranous positivity in lymphoid cells.

Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Analysis in human cell line U-251 MG and human cell line MCF-7.

Staining of human uterine cervix shows moderate membranous positivity in squamous epithelial cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL13318
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CD44 Antibody (CL13318) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CD44 Antibody (CL13318) - Azide and BSA Free (NBP3-18580) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P16070, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for CD44 Antibody (CL13318) - Azide and BSA Free

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD44