CD44 Antibody (9A4) - variant 6 - BSA Free

• Reactivity: Mu
• Applications: WB, ELISA, Flow, IHC
• Clone: 9A4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD44 Antibody (9A4) - variant 6 - BSA Free Summary

• Description: Novus Biologicals Rat CD44 Antibody (9A4) - variant 6 - BSA Free (NB100-64818) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-CD44 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: GST-CD44v6 fusion protein
• Marker: Cell membrane Marker
• Specificity: Recognizes an epitope encoded by exon v6 on the variant portion of murine CD44.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CD44
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Use in FLOW reported in scientific literature (PMID: 30504836).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: <0.1% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD44 Antibody (9A4) - variant 6 - BSA Free

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (NB100-64818)(1)

Publication using NB100-64818

• Choi SH, Kim AR, Nam JK, et al. Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization. Nat Commun. 2018-11-30 [PMID: 30504836] (IF/IHC, FLOW, WB, Mouse)

FAQs for CD44 Antibody (NB100-64818). (Showing 1 - 1 of 1 FAQ).

1. We used the CD44 variant 6 antibody (9A4, NB100-64818) in our Lab. I did not find the molecular weight of this antibody on the data sheet or in the related literature. I would appreciate it if you could give me some information about the molecular weight of this antibody.
   • The lab has informed me that on testing of the immunogen the protein is expressed at 80-85 kDa, but when tested on endogenous samples the protein is picked up at approx. 200 kDa. Most likely the protein forms a dimer, or complex when picked up in endogenous samples. Additionally, I found a paper, PMC1853452 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1853452/), that shows CD44v6 should be expressed at approx. 200 kDa - 250 kDa.

Bioinformatics

• Gene Symbol: CD44
• Entrez:
  • 12505(Mouse)
• Uniprot:
  • P15379(Mouse)