CD4 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CD4 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4 (R&D Systems, Catalog # 514-CD)
  Lys26-Trp390
  Accession # P01730
• Specificity: Detects human CD4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD4 Antibody [Biotin]

• CD_antigen: CD4
• CD4 antigen (p55)
• CD4 antigen
• CD4 molecule
• CD4 receptor
• CD4
• CD4mut
• T-cell surface antigen T4/Leu-3
• T-cell surface glycoprotein CD4

Background

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD4 Antibody (BAF379)(4)

Publications using BAF379

• Yariv Mazor Enhancement of Immune Effector Functions by Modulating IgG's Intrinsic Affinity for Target Antigen PLoS ONE, 2016-06-20;11(6):e0157788. 2016-06-20 [PMID: 27322177] (Bioassay, Human)
• Komori M, Blake A, Greenwood M et al. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis. Ann Neurol 7/1/2015 [PMID: 25808056]
• Nezlin R, Bengtsson AA Presence of IgG-CD4 complexes in the circulation. Immunol. Invest., 2008-01-01;37(2):153-62. 2008-01-01 [PMID: 18300040] (Dot Blot, Human)
• Reynard F, Willkomm N, Fatmi A Characterization of the antibody response elicited by HIV-1 Env glycomutants in rabbits. Vaccine, 2006-08-07;25(3):535-46. 2006-08-07 [PMID: 16934377] (ELISA Development, Human)

FAQs for CD4 Antibody (BAF379). (Showing 1 - 1 of 1 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: CD4
• Uniprot:
  • P01730()