CD4 Antibody [Unconjugated]


CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and more
CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room more
CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 1.7 µg/mL for 1 hour at more
Western blot shows lysates of PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
Simple Western lane view shows lysates of Human PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD4 at approximately 65 kDa (as indicated) more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF

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CD4 Antibody [Unconjugated] Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
Accession # P01730
Detects human CD4 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
AF-379-NA in the following applications:

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AF-379-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD4 Antibody [Unconjugated]

  • CD_antigen: CD4
  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4


CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

  1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD4 Antibody (AF-379-NA)(13)

We have publications tested in 3 confirmed species: Human, Primate, Primate - Cholrocebus pygerythrus (Vervet Monkey).

We have publications tested in 7 applications: Flow Cyomtetry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.

Filter By Application
Flow Cyomtetry
Western Blot
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Filter By Species
Primate - Cholrocebus pygerythrus (Vervet Monkey)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF-379-NA Applications Species
R Haddad, O Zlotnik, T Goshen-Lag, M Levi, E Brook, B Brenner, Y Kundel, I Ben-Aharon, H Kashtan Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma Journal of personalized medicine, 2022-04-13;12(4):. 2022-04-13 [PMID: 35455743] (IHC, Human) IHC Human
K Sangare, S Helmold Ha, M Moore, C Hogge, T Hoang, MA Rahman, DJ Venzon, C LaBranche, D Montefiori, M Robert-Gur, MA Thomas E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses Vaccines, 2022-02-15;10(2):. 2022-02-15 [PMID: 35214753] (Flow Cyomtetry, Western Blot, Human) Flow Cyomtetry, Western Blot Human
BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema Biology, 2021-09-18;10(9):. 2021-09-18 [PMID: 34571811] (IHC, Human) IHC Human
JP Sutherland, M Steain, ME Buckland, M Rodriguez, AL Cunningham, B Slobedman, A Abendroth Persistence of a T Cell Infiltrate in Human Ganglia Years After Herpes Zoster and During Post-herpetic Neuralgia Front Microbiol, 2019-09-11;10(0):2117. 2019-09-11 [PMID: 31572325] (IHC-P, Human) IHC-P Human
Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis. J Clin Invest, 2014-12-22;125(2):739-51. 2014-12-22 [PMID: 25562322] (IHC-Fr, Human) IHC-Fr Human
Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells. J Virol, 2014-07-09;88(19):11045-55. 2014-07-09 [PMID: 25008924] (Neutralization, Human) Neutralization Human
Suzuki , Yasuhiro, Gatanaga , Hiroyuki, Tachikawa , Natsuo, Oka , Shinichi Slow turnover of HIV-1 receptors on quiescent CD4+ T cells causes prolonged surface retention of gp120 immune complexes in vivo. PLoS ONE, 2014-02-06;9(2):e86479. 2014-02-06 [PMID: 24516533] (ICC, Human) ICC Human
Swaminathan G, Rossi F, Sierra L, Gupta A, Navas-Martin S, Martin-Garcia J A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages. PLoS Pathog, 2012-09-20;8(9):e1002937. 2012-09-20 [PMID: 23028330] (ICC, Human) ICC Human
Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes. J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129. 2012-07-11 [PMID: 22795370] (IHC, Human) IHC Human
Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, Lundberg IE, Malmstrom V T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells. J. Immunol., 2009-09-14;183(7):4792-9. 2009-09-14 [PMID: 19752224] (IHC, Human) IHC Human
Show All 13 Publications.

Review for CD4 Antibody (AF-379-NA) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Porcine.

Reviews using AF-379-NA:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD4 AF-379-NA
reviewed by:
Verified Customer
IHC-Fr Porcine 12/03/2020


Sample TestedPorcine spleen


CommentsPig spleen frozen OCT tissues were sectioned at 7µm and fixed in ice cold acetone for 7min. Once dry, blocking buffer 4% FCS/TBS was added to all tissues for 30min to minimize non-specific binding, followed by 10 min incubation with Bloxall, endogenous peroxidase blocking solution at room temperature. Tissues were washed in Tris buffered saline (TBS) for 5 min and incubated for 20min with normal horse serum (Vector laboratories # S-2012-50). Human Polyclonal goat anti-CD4 (Novus biologicals # AF379-NA) was diluted at 1:50 in 0.5% FCS/TBS and added to the arterial tissues for 1 hour incubation at room temperature. Following 5 min wash in TBS, tissues were incubated for 30min with ImmPRESS®HRP horse anti-goat reagent (Vector laboratories # MP-7405). To detect CD4 antigen, tissues were washed in TBS and incubated with VIP HRP substrate (Vector®VIP Substrate kit, Peroxidase (HRP) # SK-4600) for 1 min 30sec then placed immediately in MilliQ water to stop the reaction. Nuclei were counter-stained with methyl green (Sigma-Aldrich # M8884-25G). Control tissues were stained similarly in the absence of the primary antibody. Tissue sections were immediately dehydrated and mounted with ENTELLAN mounting medium (Proscitech, Cat#IA013). Images were captured on Nikon eclipse ME600 microscope and DS-Fi2 camera.

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FAQs for CD4 Antibody (AF-379-NA). (Showing 1 - 1 of 1 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at with any questions regarding this program.

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Blogs on CD4. Showing 1-10 of 23 blog posts - Show all blog posts.

Is Monkeypox Still A Threat?
By Jamshed Arslan, Pharm D, PhD Monkeypox is not deadly like its cousin, smallpox, nor is it as contagious as COVID-19. Yet, it continues to scare the world. In May 2022, a multinational outbreak of a cont...  Read full blog post.

Synthetic Biotic Medicine as Immunotherapy Against Cancer: Evidence From Arginine-Producing Engineered Bacteria
By Jamshed Arslan, Pharm D, PhDWhat do nuts, dairy and red meat have in common? In addition to the fact that they are all edible, one of the answers is L-arginine. This amino acid improves T cell’s respons...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

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Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Flow Cytometry Basics for the Non-Expert
By Jody Bonnevier, PhD.Flow cytometry: A Challenging Interdisciplinary ApplicationFlow cytometry has long been the cell analysis method of choice for immunologists, however more and more disciplines have recognize...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

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Recent Reviews


Verified Customer
Application: IHC-Fr
Species: Porcine


Gene Symbol CD4