Mouse CD31/PECAM-1 ELISA Kit (Colorimetric)

ELISA: CD31/PECAM-1 ELISA Kit [HRP] [KA1509] - This standard curve was generated for demonstration purpose only. A standard curve must be run with each assay.

• Reactivity: Mu
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Supernatant, Plasma (EDTA, Heparin), Serum
• Standard Curve Range: 156 to 10000 pg/mL
• Sensitivity: <10 pg/mL

Mouse CD31/PECAM-1 ELISA Kit (Colorimetric) Summary

• Description: Pecam1 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Pecam1.
• Standard Curve Range: 156 to 10000 pg/mL
• Sensitivity: <10 pg/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PECAM1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse CD31/PECAM-1 ELISA Kit (Colorimetric)

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CD31/PECAM-1 ELISA Kit (KA1509). (Showing 1 - 1 of 1 FAQ).

1. I was wondering what the shelf life of KA1509 was? Also, is the format such that you have to use the entire plate at a time or does the plate come in strips allowed multiple experiments to be performed with the same kit?
   • This kit has a shelf life of 6 months, stored at 4C. Also, the kit is supplied as a 96-well plate, and you will have to use an entire plate.

Bioinformatics

• Gene Symbol: PECAM1