CD31/PECAM-1 Antibody (C31.3 + JC/70A)

Western Blot: CD31/PECAM-1 Antibody (C31.3 + JC/70A) [NBP2-34283] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31.3 + JC/70A).

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + JC/70A) [NBP2-34283] - Formalin-paraffin human Angiosarcoma stained with CD31/PECAM-1 Antibody (C31.3 + JC/70A).

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + JC/70A) [NBP2-34283] - Formalin-paraffin human Colon Carcinoma stained with CD31/PECAM-1 Antibody (C31.3 + JC/70A).

• Reactivity: Hu, Pm-Cm, Rb
• Applications: WB, IHC
• Clone: C31.3 + JC/70A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD31/PECAM-1 Antibody (C31.3 + JC/70A) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34578)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human recombinant CD31/PECAM-1 protein (C31.3) & Membrane preparation of a spleen from a patient with hairy cell leukemia (JC/70A) (Uniprot: P16284)
• Localization: Cell surface and cytoplasm of endothelial cells
• Marker: Endothelial Cell Marker
• Specificity: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 monoclonal antibody reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
• Isotype: IgG1 Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PECAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID:33953718)
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Theoretical MW: 82.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33953718)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD31/PECAM-1 Antibody (C31.3 + JC/70A)

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (NBP2-34283)(3)

Publications using NBP2-34283

• Farina A, Rosato E, York M et al. Innate Immune Modulation Induced by EBV Lytic Infection Promotes Endothelial Cell Inflammation and Vascular Injury in Scleroderma Frontiers in Immunology 2021-04-19 [PMID: 33953718] (IF/IHC, Human)
• Barron AMS, Mantero JC, Ho JD et al. Perivascular Adventitial Fibroblast Specialization Accompanies T Cell Retention in the Inflamed Human Dermis. J Immunol. 2019-01-01 [PMID: 30510068] (IHC-P, Human)
  
  Details:
  Citation using the Azide and BSA Free form of this antibody.
• Nazari B, Rice LM, Stifano G et al. Altered Dermal Fibroblasts in Systemic Sclerosis Display Podoplanin and CD90. Am. J. Pathol. 2016-08-23 [PMID: 27565038] (IF/IHC)

FAQs for CD31/PECAM-1 Antibody (NBP2-34283). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of the antibody catalog number: NBP2-34283-0.1ml?
   • The concentration for this item is 0.2mg/ml.

Bioinformatics

• Gene Symbol: PECAM1
• Uniprot:
  • P16284()
  • P16284()
  • P16284()