CCR7 Antibody (150503) [Biotin]

Human peripheral blood mononuclear cells (PBMCs) were stained with PE-conjugated Mouse anti-Human CD4 Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse anti-Human CCR7 Biotinylated Monoclonal Antibody (Catalog # FAB197B) or (B) Mouse IgG2A isotype control antibody (Catalog # IC003B) followed by APC-conjugated Streptavidin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 150503
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

CCR7 Antibody (150503) [Biotin] Summary

• Additional Information: Clone 150503 was used by HLDA to establish CD designation
• Immunogen: Human CCR7 transfectants
  Met1-Pro378
  Accession # AAA58615
• Specificity: Detects human CCR7.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR7 Antibody (150503) [Biotin]

• BLR2
• BLR2C-C chemokine receptor type 7
• C-C CKR-7
• CC-CKR-7
• CCR7
• CCR-7
• CD197 antigen
• CD197
• CDw197
• CDw197CC chemokine receptor 7
• chemokine (C-C motif) receptor 7
• CMKBR7
• CMKBR7chemokine (C-C) receptor 7
• EBI1
• EBI1EBV-induced G protein-coupled receptor 1
• EBV-induced G-protein coupled receptor 1
• Epstein-Barr virus induced gene 1
• Epstein-Barr virus induced G-protein coupled receptor
• Epstein-Barr virus-induced G-protein coupled receptor 1
• EVI1
• lymphocyte-specific G protein-coupled peptide receptor
• MIP-3 beta receptor

Background

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR7 Antibody (FAB197B)(2)

Publications using FAB197B

• F Barrenäs, SG Hansen, L Law, C Driscoll, RR Green, E Smith, J Chang, I Golez, T Urion, X Peng, L Whitmore, D Newhouse, CM Hughes, D Morrow, KT Randall, AN Selseth, JC Ford, RM Gilbride, BE Randall, E Ainslie, K Oswald, R Shoemaker, R Fast, WJ Bosche, MK Axthelm, Y Fukazawa, GN Pavlakis, BK Felber, S Fourati, RP Sekaly, JD Lifson, J Komorowski, E Kosmider, D Shao, W Song, PT Edlefsen, LJ Picker, M Gale Interleukin-15 response signature predicts RhCMV/SIV vaccine efficacy PloS Pathogens, 2021-07-06;17(7):e1009278. 2021-07-06 [PMID: 34228762] (Flow Cytometry, Rhesus Macaque)
• Meythaler M, Wang Z, Martinot A, Pryputniewicz S, Kasheta M, McClure HM, O'Neil SP, Kaur A Early Induction of Polyfunctional Simian Immunodeficiency Virus (SIV)-Specific T Lymphocytes and Rapid Disappearance of SIV from Lymph Nodes of Sooty Mangabeys during Primary Infection. J. Immunol., 2011-03-25;186(9):5151-61. 2011-03-25 [PMID: 21441446] (Flow Cytometry, Primate - Cercocebus atys (Sooty Mangabey))

Bioinformatics

• Uniprot:
  • AAA58615()