EVI-1 Antibody

Images

 
Western Blot: EVI-1 Antibody [NBP2-48848] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: EVI-1 Antibody [NBP2-48848] - Staining of human cell line HEK 293 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
Immunohistochemistry: EVI-1 Antibody [NBP2-48848] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

EVI-1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEY
Specificity
Specificity of human EVI-1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MECOM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
EVI-1 Recombinant Protein Antigen (NBP2-48848PEP)

Reactivity Notes

Mouse (86%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EVI-1 Antibody

  • AML1-EVI-1 fusion protein
  • AML1-EVI-1
  • ecotropic viral integration site 1
  • Ecotropic virus integration site 1 protein homolog
  • EVI1
  • EVI-1
  • EVI1MDS1-EVI1
  • MDS1 and EVI1 complex locus protein EVI1
  • MDS1 and EVI1 complex locus protein MDS1
  • MDS1 and EVI1 complex locus
  • MDS1
  • MECOM
  • MGC163392
  • MGC97004
  • Myelodysplasia syndrome 1 protein
  • myelodysplasia syndrome 1
  • Myelodysplasia syndrome-associated protein 1
  • oncogene EVI1
  • PRDM3
  • zinc finger protein Evi1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MECOM