EVI-1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: EVI-1 Antibody [NBP2-48848] - Staining of human cell line HEK 293 shows localization to nuclear speckles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Western Blot: EVI-1 Antibody [NBP2-48848] -Analysis in human cell line RT-4.

ChIP-Exo-Seq composite graph for Anti-MECOM (NBP2-48848) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Validation of MECOM expression levels in clinical KIRC samples. A Relative MECOM mRNA levels in tumor and adjacent normal tissues of 24 patients with KIRC. B MECOM mRNA differential expression in 24 patients with KIRC between tumor and adjacent normal tissues. C Representative images of MECOM IHC staining in tumor and adjacent normal tissues in patients with various T stages (n = 147). D IHC scores of MECOM expression in tumor tissues of patients with KIRC at various clinical stages. E MECOM protein levels in tumor and adjacent normal tissues of eight patients of KIRC Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39576394), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EVI-1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: ATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MECOM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10ug per reaction
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  EVI-1 Recombinant Protein Antigen (NBP2-48848PEP)

Reactivity Notes

Mouse (86%), Rat (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EVI-1 Antibody - BSA Free

• AML1-EVI-1 fusion protein
• AML1-EVI-1
• ecotropic viral integration site 1
• Ecotropic virus integration site 1 protein homolog
• EVI1
• EVI-1
• EVI1MDS1-EVI1
• MDS1 and EVI1 complex locus protein EVI1
• MDS1 and EVI1 complex locus protein MDS1
• MDS1 and EVI1 complex locus
• MDS1
• MECOM
• MGC163392
• MGC97004
• Myelodysplasia syndrome 1 protein
• myelodysplasia syndrome 1
• Myelodysplasia syndrome-associated protein 1
• oncogene EVI1
• PRDM3
• zinc finger protein Evi1

Background

EVI1 (ecotropic viral integration site 1) was originally identified as a gene located in the integration site of ecotropic retroviruses in the mouse genome that resulted in myeloid tumors. EVI1 is a zinc finger transcription factor that plays an important role in development and leukemogenesis. It is an oncogene that can have effects on cell proliferation, differentiation, and apoptosis. Chromosomal translocations involving EVI1 result in chronic myelogenous leukemia (CML). Alternate names for EVI1 include AML1-EVI-1 fusion protein, PRDM3, and MDS1-EV1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EVI-1 Antibody (NBP2-48848)(2)

Publications using NBP2-48848

• Lu Y, Quan J, Liu F et Al. Systematic pan-cancer analysis of the prognostic value of MECOM in human cancer Discov Oncol 2024-11-22 [PMID: 39576394]
• Pradeepa , Suresh V, Senapati S, Chakraborty S AKT inhibition sensitizes EVI1 expressing colon cancer cells to irinotecan therapy by regulating the Akt/mTOR axis Cellular oncology (Dordrecht) 2022-07-14 [PMID: 35834097]

Bioinformatics

• Gene Symbol: MECOM