Recombinant Human CCL19/MIP-3 beta Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human CCL19/MIP-3 beta Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED₅₀ for this effect is 3-15 ng/mL.
• Source: E. coli-derived human CCL19/MIP-3 beta protein
  Gly22-Ser98
• Accession #: Q99731.1
• N-terminal Sequence: Gly22
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL19
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 8.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL19/MIP-3 beta Protein

• beta chemokine exodus-3
• Beta-chemokine exodus-3
• CC chemokine ligand 19
• C-C motif chemokine 19
• CCL19
• chemokine (C-C motif) ligand 19
• CKb11
• EBI1-ligand chemokine
• ELC
• ELCMIP-3-beta
• Epstein-Barr virus-induced molecule 1 ligand chemokine
• exodus-3
• Macrophage inflammatory protein 3 beta
• macrophage inflammatory protein 3-beta
• MGC34433
• MIP3 beta
• MIP-3 beta
• MIP-3b
• MIP3BCK beta-11
• SCYA19EBI1 ligand chemokine
• small inducible cytokine subfamily A (Cys-Cys), member 19
• Small-inducible cytokine A19

Background

MIP-3 beta , also known as ELC (EBI1-Ligand Chemokine), is one of many novel beta chemokines identified through bioinformatics. MIP-3 beta cDNA encodes a 98 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is cleaved to form the 77 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity) and the gene for MIP-3 beta  has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for cultured human lymphocytes. MIP-3 beta has also been shown to be a unique functional ligand for CCR-7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. EBI1 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

1. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Publications for CCL19/MIP-3 beta (361-MI)(43)

Publications using 361-MI

• Cardona, CI;Rodriguez, A;Torres, VC;Sanchez, A;Torres, A;Vazquez, AE;Wagler, AE;Brissette, MA;Bill, CA;Vines, CM; C-C Chemokine Receptor 7 Promotes T-Cell Acute Lymphoblastic Leukemia Invasion of the Central Nervous System via ?2-Integrins International journal of molecular sciences 2024-09-06 [PMID: 39273598] (Bioassay, Human)
• Tencer, AH;Yu, Y;Causse, SZ;Campbell, GR;Klein, BJ;Xuan, H;Cartier, J;Miles, MA;Gaurav, N;Zadoroznyj, A;Holt, TA;Wen, H;Hawkins, CJ;Spector, SA;Dubrez, L;Shi, X;Kutateladze, TG; Molecular basis for nuclear accumulation and targeting of the inhibitor of apoptosis BIRC2 Nature structural & molecular biology 2023-07-31 [PMID: 37524969] (Bioassay, Human)
• Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function PLoS ONE, 2023-01-20;18(1):e0280590. 2023-01-20 [PMID: 36662882] (Bioassay, Human)
• DA Covino, MG Desimio, M Doria Impact of IL-15 and latency reversing agent combinations in the reactivation and NK cell-mediated suppression of the HIV reservoir Scientific Reports, 2022-11-03;12(1):18567. 2022-11-03 [PMID: 36329160] (Bioassay, Human)
• W Piao, L Li, V Saxena, J Iyyathurai, R Lakhan, Y Zhang, IT Lape, C Paluskievi, KL Hippen, Y Lee, E Silverman, MW Shirkey, LV Riella, BR Blazar, JS Bromberg PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration Nature Communications, 2022-04-21;13(1):2176. 2022-04-21 [PMID: 35449134] (Bioassay, Human)
• EP Brandum, AS Jørgensen, MB Calvo, K Spiess, FC Peterson, Z Yang, BF Volkman, CT Veldkamp, MM Rosenkilde, CK Goth, GM Hjortø Selective Boosting of CCR7-Acting Chemokines; Short Peptides Boost Chemokines with Short Basic Tails, Longer Peptides Boost Chemokines with Long Basic Tails International Journal of Molecular Sciences, 2022-01-26;23(3):. 2022-01-26 [PMID: 35163323] (Bioassay, Human)
• Madalena Cipriano, Katharina Schlünder, Christopher Probst, Kirstin Linke, Martin Weiss, Mona Julia Fischer et al. Human immunocompetent choroid-on-chip: a novel tool for studying ocular effects of biological drugs Communications Biology 2022-01-13 [PMID: 35027657] (Bioassay, Human)
• K Kurowarabe, M Endo, D Kobayashi, H Hayasaka CXCL12-stimulated lymphocytes produce secondary stimulants that affect the surrounding cell chemotaxis Biochemistry and Biophysics Reports, 2021-09-07;28(0):101128. 2021-09-07 [PMID: 34527817] (Bioassay, Human)
• GR Campbell, J Zhuang, G Zhang, I Landa, LJ Kubiatowic, D Dehaini, RH Fang, L Zhang, SA Spector CD4+ T cell-mimicking nanoparticles encapsulating DIABLO/SMAC mimetics broadly neutralize HIV-1 and selectively kill HIV-1-infected cells Theranostics, 2021-08-25;11(18):9009-9021. 2021-08-25 [PMID: 34522224] (Bioassay, Human)
• H Dhandapani, H Jayakumar, A Seetharama, SS Singh, S Ganeshraja, N Jagadish, A Suri, R Thangaraja, P Ramanathan Dendritic cells matured with recombinant human sperm associated antigen 9 (rhSPAG9) induce CD4+, CD8+ T cells and activate NK cells: a potential candidate molecule for immunotherapy in cervical cancer Cancer Cell International, 2021-09-07;21(1):473. 2021-09-07 [PMID: 34493268] (Bioassay, Human)
• T Liang, Q Zhang, Z Wu, P Chen, Y Huang, S Liu, L Li UHRF1 Suppresses HIV-1 Transcription and Promotes HIV-1 Latency by Competing with p-TEFb for Ubiquitination-Proteasomal Degradation of Tat MBio, 2021-08-31;12(4):e0162521. 2021-08-31 [PMID: 34465029] (Bioassay, Human)
• G Zhang, GR Campbell, Q Zhang, E Maule, J Hanna, W Gao, L Zhang, SA Spector CD4+ T Cell-Mimicking Nanoparticles Broadly Neutralize HIV-1 and Suppress Viral Replication through Autophagy MBio, 2020-09-15;11(5):. 2020-09-15 [PMID: 32934078] (Cell Culture, Human)
• G Khoury, C Mackenzie, L Ayadi, SR Lewin, C Branlant, DFJ Purcell Tat IRES modulator of tat mRNA (TIM-TAM): a conserved RNA structure that controls Tat expression and acts as a switch for HIV productive and latent infection Nucleic Acids Res., 2020-03-18;0(0):. 2020-03-18 [PMID: 31875221] (Cell Culture, Human)
• Erica Giuliani, Maria Giovanna Desimio, Margherita Doria Hexamethylene bisacetamide impairs NK cell-mediated clearance of acute T lymphoblastic leukemia cells and HIV-1-infected T cells that exit viral latency Scientific Reports 2019-03-13 [PMID: 30867508] (Bioassay, Human)
• S Caielli, DT Veiga, P Balasubram, S Athale, B Domic, E Murat, R Banchereau, Z Xu, M Chandra, CH Chung, L Walters, J Baisch, T Wright, M Punaro, L Nassi, K Stewart, J Fuller, D Ucar, H Ueno, J Zhou, J Banchereau, V Pascual A CD4+ T cell population expanded in lupus blood provides B cell help through interleukin-10 and succinate Nat. Med., 2018-11-26;0(0):. 2018-11-26 [PMID: 30478422] (Bioassay, Human)
• H Plešingero, P Janovská, A Mishra, L Smy?ková, L Poppová, A Libra, K Plevová, P Ovesná, L Radová, M Doubek, Š Pavlová, Š Pospíšilov, V Bryja Expression of COBLL1 encoding novel ROR1 binding partner is robust predictor of survival in chronic lymphocytic leukemia Haematologica, 2017-11-09;0(0):. 2017-11-09 [PMID: 29122990] (Bioassay, Human)
• D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017-08-17;7(1):8536. 2017-08-17 [PMID: 28819198] (Bioassay, Human)
• JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use J Immunol Res, 2017-07-13;2017(0):1975902. 2017-07-13 [PMID: 28785596] (Bioassay, Human)
• B Xu, M Zhou, W Qiu, J Ye, Q Feng CCR7 mediates human breast cancer cell invasion, migration by inducing epithelial-mesenchymal transition and suppressing apoptosis through AKT pathway Cancer Med, 2017-04-04;0(0):. 2017-04-04 [PMID: 28378417] (Bioassay, Human)
• RA Raposo, M de Mulder, D Paquin-Pro, PM Brailey, VD Cabido, PM Zdinak, AS Thomas, SH Huang, GA Beckerle, RB Jones, DF Nixon IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis JCI Insight, 2017-01-12;2(1):e85811. 2017-01-12 [PMID: 28097226] (Bioassay, Human)
• Dengue virus infection induces interferon-lambda1 to facilitate cell migration Sci Rep, 2016-07-26;6(0):24530. 2016-07-26 [PMID: 27456172] (Bioassay, Human)
• Human breast cancer-derived soluble factors facilitate CCL19-induced chemotaxis of human dendritic cells Sci Rep, 2016-07-25;6(0):30207. 2016-07-25 [PMID: 27451948] (Bioassay, Human)
• RB Jones, S Mueller, R O'Connor, K Rimpel, DD Sloan, D Karel, HC Wong, EK Jeng, AS Thomas, JB Whitney, SY Lim, C Kovacs, E Benko, S Karandish, SH Huang, MJ Buzon, M Lichterfel, A Irrinki, JP Murry, A Tsai, H Yu, R Geleziunas, A Trocha, MA Ostrowski, Irvi A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes PLoS Pathog, 2016-04-15;12(4):e1005545. 2016-04-15 [PMID: 27082643] (Bioassay, Human)
• Janovska P, Poppova L, Plevova K, Plesingerova H, Behal M, Kaucka M, Ovesna P, Hlozkova M, Borsky M, Stehlikova O, Brychtova Y, Doubek M, Machalova M, Baskar S, Kozubik A, Pospisilova S, Pavlova S, Bryja V Autocrine Signaling by Wnt-5a Deregulates Chemotaxis of Leukemic Cells and Predicts Clinical Outcome in Chronic Lymphocytic Leukemia. Clin Cancer Res, 2015-08-03;22(2):459-69. 2015-08-03 [PMID: 26240275] (Bioassay, Human)
• Kaucka M, Petersen J, Janovska P, Radaszkiewicz T, Smyckova L, Daulat A, Borg J, Schulte G, Bryja V Asymmetry of VANGL2 in migrating lymphocytes as a tool to monitor activity of the mammalian WNT/planar cell polarity pathway. Cell Commun Signal, 2015-01-28;13(0):2. 2015-01-28 [PMID: 25627785] (Bioassay, Human)
• Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection. PLoS Pathog, 2014-12-04;10(12):e1004548. 2014-12-04 [PMID: 25474532] (Bioassay, Human)
• Ramachandran M, Jin C, Yu D, Eriksson F, Essand M Vector-encoded Helicobacter pylori neutrophil-activating protein promotes maturation of dendritic cells with Th1 polarization and improved migration. J Immunol, 2014-07-21;193(5):2287-96. 2014-07-21 [PMID: 25049358] (Bioassay, Human)
• Hofbauer , Sebastia, Krenn , Peter W, Ganghammer , Sylvia, Asslaber , Daniela, Pichler , Ulrike, Oberascher , Karin, Henschler , Reinhard, Wallner , Michael, Kerschbaum , Hubert, Greil , Richard, Hartmann , Tanja N Tiam1/Rac1 signals contribute to the proliferation and chemoresistance, but not motility, of chronic lymphocytic leukemia cells. Blood, 2014-02-05;123(14):2181-8. 2014-02-05 [PMID: 24501217] (Bioassay, Human)
• Spina , Celsa A, Anderson , Jenny, Archin , Nancie M, Bosque , Alberto, Chan , Jonathan, Famiglietti , Marylind, Greene , Warner C, Kashuba , Angela, Lewin , Sharon R, Margolis , David M, Mau , Matthew, Ruelas , Debbie, Saleh , Suha, Shirakawa , Kotaro, Siliciano , Robert F, Singhania , Akul, Soto , Paula C, Terry , Valeri H, Verdin , Eric, Woelk , Christop, Wooden , Stacey, Xing , Sifei, Planelles , Vicente An in-depth comparison of latent HIV-1 reactivation in multiple cell model systems and resting CD4+ T cells from aviremic patients. PLoS Pathog, 2013-12-26;9(12):e1003834. 2013-12-26 [PMID: 24385908] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Napoletano C, Zizzari IG, Rughetti A, Rahimi H, Irimura T, Clausen H, Wandall HH, Belleudi F, Bellati F, Pierelli L, Frati L, Nuti M Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation. Eur. J. Immunol., 2012-04-01;42(4):936-45. 2012-04-01 [PMID: 22531918] (Bioassay, Human)
• Beck B, Dorfel D, Lichtenegger FS, Geiger C, Lindner L, Merk M, Schendel DJ, Subklewe M Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission. J Transl Med, 2011-09-13;9(0):151. 2011-09-13 [PMID: 21910911] (Bioassay, Human)
• Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia. Ann. Neurol., 2009-10-01;66(4):521-31. 2009-10-01 [PMID: 19847900] (ELISA (Standard))
• Damas JK, Landro L, Fevang B, Heggelund L, Tjonnfjord GE, Floisand Y, Halvorsen B, Froland SS, Aukrust P Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication. Clin. Exp. Immunol., 2009-09-01;157(3):400-7. 2009-09-01 [PMID: 19664149] (Bioassay, Human)
• Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation. Blood, 2008-05-14;112(3):493-503. 2008-05-14 [PMID: 18480427] (Bioassay, Human)
• Lim HW, Kim CH Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue. J. Immunol., 2007-12-01;179(11):7448-56. 2007-12-01 [PMID: 18025189] (Bioassay, Human)
• Bayry J, Triebel F, Kaveri SV, Tough DF Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells. J. Immunol., 2007-04-01;178(7):4184-93. 2007-04-01 [PMID: 17371975] (Bioassay, Human)
• Kriegova E, Tsyrulnyk A, Arakelyan A, Mrazek F, Ordeltova M, Petzmann S, Zatloukal J, Kolek V, du Bois RM, Popper H, Petrek M Expression of CCX CKR in pulmonary sarcoidosis. Inflamm. Res., 2006-10-01;55(10):441-5. 2006-10-01 [PMID: 17109071] (Bioassay, Human)
• Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. J. Immunol., 2006-10-01;177(7):4488-94. 2006-10-01 [PMID: 16982885] (Bioassay, Human)
• Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006-04-17;350(2):484-92. 2006-04-17 [PMID: 16603217] (Binding Assay, Bioassay, Virus)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Bioassay, Human)
• Curiel , Tyler J, Coukos , George, Zou , Linhua, Alvarez , Xavier, Cheng , Pui, Mottram , Peter, Evdemon-Hogan , Melina, Conejo-Garcia , Jose R, Zhang , Lin, Burow , Matthew, Zhu , Yun, Wei , Shuang, Kryczek , Ilona, Daniel , Ben, Gordon , Alan, Myers , Leann, Lackner , Andrew, Disis , Mary L, Knutson , Keith L, Chen , Lieping, Zou , Weiping Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Nat Med, 2004-08-22;10(9):942-9. 2004-08-22 [PMID: 15322536] (Bioassay, Human)
• Chang WL, Baumgarth N, Yu D, Barry PA Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. J. Virol., 2004-08-01;78(16):8720-31. 2004-08-01 [PMID: 15280480] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CCL19
• Uniprot:
  • Q99731.1()