CCL21/6Ckine Antibody

Images

 
CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457) at 5 µg/mL overnight at 4 °C. Tissue was ...read more
D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more
Recombinant Mouse CCL21/6Ckine (Catalog # 457‑6C) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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CCL21/6Ckine Antibody Summary

Immunogen
E. coli-derived recombinant mouse CCL21/6Ckine
Ser24-Gly133
Accession # P84444
Specificity
Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross reactivity with recombinant rat CCL21/6Ckine is observed and less than 15% cross-reactivity with recombinant human CCL21/6Ckine is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Ccl21a
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.5-2.5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF457 in the following applications:

Publications
Read Publications using
AF457 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL21/6Ckine Antibody

  • 6Ckine
  • 6CkineSmall-inducible cytokine A21
  • Beta-chemokine exodus-2
  • CCL21
  • chemokine (C-C motif) ligand 21
  • CKb9
  • exodus-2
  • member 21
  • SCYA21
  • SCYA21MGC34555
  • secondary lymphoid tissue chemokine
  • SLC
  • SLCSecondary lymphoid-tissue chemokine
  • TCA-4

Background

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21-33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta /ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines. Mature rat CCL21 shares 84% aa sequence identity with mouse CCL21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL21/6Ckine Antibody (AF457)(41)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(3)
Functional Assay
(2)
ICC
(2)
IHC
(13)
IHC-Fr
(8)
IHC-P
(4)
Neutralization
(5)
Western Blot
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(37)
Rat
(1)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using AF457 Applications Species
SY Choi, H Bae, SH Jeong, I Park, H Cho, SP Hong, DH Lee, CK Lee, JS Park, SH Suh, J Choi, MJ Yang, JY Jang, L Onder, JH Moon, HS Jeong, RH Adams, JM Kim, B Ludewig, JH Song, DS Lim, GY Koh YAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells Nat Commun, 2020;11(1):519. 2020 [PMID: 31980640] (IHC, Mouse) IHC Mouse
X Jia, F Gábris, Ó Jacobsen, G Bedics, B Botz, Z Helyes, Z Kellermaye, D Vojkovics, G Berta, N Nagy, Z Jakus, P Balogh Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas J. Immunol., 2019;0(0):. 2019 [PMID: 31767783] (Flow Cytometry, Mouse) Flow Cytometry Mouse
HW Cheng, L Onder, M Novkovic, C Soneson, M Lütge, N Pikor, E Scandella, MD Robinson, JI Miyazaki, A Tersteegen, U Sorg, K Pfeffer, T Rülicke, T Hehlgans, B Ludewig Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp Nat Commun, 2019;10(1):1739. 2019 [PMID: 30988302] (IHC, Mouse) IHC Mouse
K Veerman, C Tardiveau, F Martins, J Coudert, JP Girard Single-Cell Analysis Reveals Heterogeneity of High Endothelial Venules and Different Regulation of Genes Controlling Lymphocyte Entry to Lymph Nodes Cell Rep, 2019;26(11):3116-3131.e5. 2019 [PMID: 30865898] (Flow Cytometry, Mouse) Flow Cytometry Mouse
M Hsu, A Rayasam, JA Kijak, YH Choi, JS Harding, SA Marcus, WJ Karpus, M Sandor, Z Fabry Neuroinflammation-induced lymphangiogenesis near the cribriform plate contributes to drainage of CNS-derived antigens and immune cells Nat Commun, 2019;10(1):229. 2019 [PMID: 30651548] (Western Blot, Mouse) Western Blot Mouse
Y Kimizuka, W Katagiri, JJ Locascio, A Shigeta, Y Sasaki, M Shibata, K Morse, RF Sîrbulescu, M Miyatake, P Reeves, M Suematsu, J Gelfand, T Brauns, MC Poznansky, K Tsukada, S Kashiwagi Brief Exposure of Skin to Near-Infrared Laser Modulates Mast Cell Function and Augments the Immune Response J. Immunol., 2018;0(0):. 2018 [PMID: 30420435] (IHC, Mouse) IHC Mouse
L Liu, L Zhao, Y Yang, J Gao, C Hu, B Guo, B Zhu Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19 Oncol Lett, 2018;16(4):5013-5019. 2018 [PMID: 30250567] (IHC, Mouse) IHC Mouse
W Piao, Y Xiong, K Famulski, CC Brinkman, L Li, N Toney, C Wagner, V Saxena, T Simon, JS Bromberg Regulation of T cell afferent lymphatic migration by targeting LT?R-mediated non-classical NF?B signaling Nat Commun, 2018;9(1):3020. 2018 [PMID: 30069025] (IHC-P, Mouse) IHC-P Mouse
CA Thomson, SA van de Pav, M Stakenborg, E Labeeuw, G Matteoli, AM Mowat, RJB Nibbs Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators J. Immunol., 2018;0(0):. 2018 [PMID: 29760193] (IHC, Mouse) IHC Mouse
D Respatika, Y Saito, K Washio, S Komori, T Kotani, H Okazawa, Y Murata, T Matozaki Role of SIRP? in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen Kobe J Med Sci, 2017;63(1):E22-E29. 2017 [PMID: 29434170] (IHC, Mouse) IHC Mouse
Show All 41 Publications.

Review for CCL21/6Ckine Antibody (AF457) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF457:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24006262
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24006262

Details

Detection NotesSee PMID 24006262

Comments

CommentsPublished: See PMID 24006262Independent review from antibodyresource.com

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Secondary Antibodies

 

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Biotin BAF457

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Recent Reviews

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Anonymous
01/08/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol Ccl21a
Uniprot