c-Myc Antibody

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Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity ...read more
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ...read more

Product Details

Summary
Reactivity Hu, Mu, Ec, NASpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

c-Myc Antibody Summary

Immunogen
Goats were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Localization
Cytoplasmic and Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:30000
  • ELISA Coating: 1:100-1:500; Primary: 1:1000-1:30000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunohistochemistry-Frozen 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
IHC-F reactivity reported in scientific literature (PMID:22411008). ICC/IF reactivity reported in scientific literature (PMID: 28368777).
Positive Control
c-Myc Lysate (NBL1-13414)
Control Peptide
c-Myc Peptide (NB600-336PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB600-335 in the following applications:

Publications
Read Publications using
NB600-335 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID; 19966808). Human reactivity reported in scientific literature (PMID: 22037249)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Myc Antibody

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC-Fr, IP

Publications for c-Myc Antibody (NB600-335)(37)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-Fr, IP, WB.


Filter By Application
ICC/IF
(6)
IHC-Fr
(1)
IP
(6)
WB
(4)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NB600-335 Applications Species
Somplatzki S, Muhlenhoff M, Kroger A et al. Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells BMC Biotechnol 2017 May 12 [PMID: 28499450] (ICC/IF, Human)

Details:
This reference used the FITC version of NB600-335.
ICC/IF Human
Gelmetti V, DeRosa P, Torosantucci L et al. PINK1 and BECN1 relocalize at mitochondria-associated membranes during mitophagy and promote ER-mitochondria tethering and autophagosome formation. Autophagy. Dec 23 2016 [PMID: 28368777] (ICC/IF, WB, IP, Human) ICC/IF, WB, IP Human
Moutel S, Bery N, Bernard V et al. NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies. Elife. 2016 Jul 19 [PMID: 27434673] (ICC/IF)

Details:
This citation used the HRP version of this antibody.
ICC/IF
Brunati M, Perucca S, Han L et al. The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin. Elife 2015 [PMID: 26673890]
Krishnaswamy A, Yamagata M, Duan X et al. Sidekick 2 directs formation of a retinal circuit that detects differential motion. Nature. 2015 Aug 27 [PMID: 26287463]
van der Kant R, Fish A, Janssen L et al. Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. J Cell Sci 2013 May 31 [PMID: 23729732] (ICC/IF) ICC/IF
Arena G, Gelmetti V, Torosantucci L et al. PINK1 protects against cell death induced by mitochondrial depolarization, by phosphorylating Bcl-xL and impairing its pro-apoptotic cleavage Cell Death Differ 2013 Mar 22 [PMID: 23519076] (WB, IP) WB, IP
Kuroiwa M, Hamada M, Hieda E et al. Muscarinic receptors acting at pre- and post-synaptic sites differentially regulate dopamine/DARPP-32 signaling in striatonigral and striatopallidal neuron Neuropharmacology 2012 Dec [PMID: 22971543] (IP)

Details:
This citation used the HRP version of this antibody.
IP
Yang SB, McLemore KD, Tasic B et al. Kv1.1-dependent Control of Hippocampal Neuron Number as Revealed by Mosaic Analysis with Double Markers (MADM). J Physiol. 2012 Apr 23. [PMID: 22411008] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Matthys A, Van Craenenbroeck K, Lintermans B et al. RhoBTB3 interacts with the 5-HT7a receptor and inhibits its proteasomal degradation Cellular Signalling 41041 [PMID: 22245496] (WB, Human) WB Human
Show All 37 Publications.

Review for c-Myc Antibody (NB600-335) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB600-335:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot c-Myc NB600-335
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reviewed by:
Costache Vasilescu
WB Human 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from 293T cells, in lysis buffer
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiondonkey anti-goat IgG-HRP (SCBT)
Secondary Manufacturer Cat##sc-2020
Secondary Concentration1:4000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 20 sec

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose Immobilized Labeled NB600-342
Biotin Labeled NB600-335B
HRP Labeled NB600-341

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Bioinformatics Tool for c-Myc Antibody (NB600-335)

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Blogs on c-Myc. Showing 1-10 of 14 blog posts - Show all blog posts.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.

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Recent Reviews

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5
1
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0
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1
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Costache Vasilescu
12/18/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MYC
Entrez