c-Myc Antibody


Western Blot: c-Myc Antibody [NB600-335] - Analysis using the Biotin conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at ...read more
Western Blot: c-Myc Antibody [NB600-335] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ...read more

Product Details

Reactivity Mu, Ec, NASpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IP
1.0 mg/ml

Order Details

Available Conjugates

c-Myc Antibody Summary

Goats were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Cytoplasmic and Nuclear
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000-1:30000
  • ELISA Coating: 1:100-1:500; Primary: 1:1000-1:30000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunohistochemistry-Frozen 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
Control Peptide
c-Myc Peptide (NB600-336PEP)
Reviewed Applications
Read 1 Review rated 5
NB600-335 in the following applications:

Read Publications using
NB600-335 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID; 19966808)

Alternate Names for c-Myc Antibody

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)


The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Myc Antibody (NB600-335)(25)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-Fr, IP, WB.

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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB600-335 Applications Species
Brunati M, Perucca S, Han L et al. The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin. Elife 2015 [PMID:26673890]
Krishnaswamy A, Yamagata M, Duan X et al. Sidekick 2 directs formation of a retinal circuit that detects differential motion. Nature. 2015 Aug 27 [PMID:26287463]
van der Kant R, Fish A, Janssen L et al. Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. J Cell Sci 2013 May 31 [PMID:23729732] (ICC/IF) ICC/IF
Arena G, Gelmetti V, Torosantucci L et al. PINK1 protects against cell death induced by mitochondrial depolarization, by phosphorylating Bcl-xL and impairing its pro-apoptotic cleavage Cell Death Differ 2013 Mar 22 [PMID:23519076] (WB, IP) WB, IP
Yang SB, McLemore KD, Tasic B et al. Kv1.1-dependent Control of Hippocampal Neuron Number as Revealed by Mosaic Analysis with Double Markers (MADM). J Physiol. 2012 Apr 23. [PMID:22411008] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Matthys A, Van Craenenbroeck K, Lintermans B et al. RhoBTB3 interacts with the 5-HT7a receptor and inhibits its proteasomal degradation Cellular Signalling 41041 [PMID:22245496] (WB, Human) WB Human
Zheng Q, Su H, Ranek MJ, Wang X. Autophagy and p62 in Cardiac Proteinopathy. Circ Res. 2011 Jun 9. [PMID:21659648] (ICC/IF, Mouse) ICC/IF Mouse
Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1 Mol Cell 2011 Jun 10 [PMID:21658608] (WB) WB
Singla V, Hunkapiller J, Santos N et al. Floxin, a resource for genetically engineering mouse ESCs. Nat Methods 2010 Jan [PMID:19966808] (Mouse) Mouse
Singaravelu K, Devalaraja-Narashimha K, Lastovica B et al. PERP, a p53 pro-apoptotic target, mediates apoptotic cell death in renal ischemia. Am J Physiol Renal Physiol :90438.92008. 2009 [PMID:19158346]
Show All 25 Publications.

Reviews for c-Myc Antibody (NB600-335) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB600-335:
Filter by Applications
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Images Ratings Applications Species Date Details
Western Blot c-Myc NB600-335
reviewed by:
WB Human 12/18/2014


ApplicationWestern Blot
Sample TestedWhole cell lysate from 293T cells, in lysis buffer


Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiondonkey anti-goat IgG-HRP (SCBT)
Secondary Manufacturer Cat##sc-2020
Secondary Concentration1:4000


Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 20 sec

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Agarose Immobilized Labeled NB600-342
Biotin Labeled NB600-335B
FITC Labeled NB600-340
HRP Labeled NB600-341

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Blogs on c-Myc. Showing 1-10 of 12 blog posts - Show all blog posts.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

The Myc Epitope Tags Along...
Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

Contact Information

Product PDFs


Gene Symbol MYC

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