c-Myc Antibody

Images

 
Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity ...read more
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ...read more
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody [NB600-335] - Immunocytochemistry/ Immunofluorescence: c-Myc Antibody [Alexa Fluor® 488] [NB600-335AF488] - HeLa cells were fixed for 10 minutes using 10% ...read more

Product Details

Summary
Reactivity Hu, Mu, Ec, NASpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

c-Myc Antibody Summary

Immunogen
Goats were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Localization
Cytoplasmic and Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:30000
  • ELISA Coating: 1:100-1:500; Primary: 1:1000-1:30000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunohistochemistry-Frozen 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
IHC-F reactivity reported in scientific literature (PMID:22411008). ICC/IF reactivity reported in scientific literature (PMID: 28368777).
Control
c-Myc Lysate (NBL1-13414)
c-Myc Lysate (NBP2-65859)
Control Peptide
c-Myc Peptide (NB600-336PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB600-335 in the following applications:

Publications
Read Publications using
NB600-335 in the following applications:

  • 6 publications
  • IHC
    1 publication
  • 1 publication
  • IP
    7 publications
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID; 19966808). Human reactivity reported in scientific literature (PMID: 22037249)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Myc Antibody

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Myc Antibody (NB600-335)(40)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
ICC/IF
(6)
IHC
(1)
IHC-Fr
(1)
IP
(7)
WB
(5)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NB600-335 Applications Species
Brunati M, Perucca S, Han L et al. The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin. Elife 2015 [PMID: 26673890]
Van Humbeeck C, Cornelissen T, Hofkens H et al. Parkin interacts with Ambra1 to induce mitophagy J Neurosci

Details:
This publication used the FITC conjugated form of this antibody (Cat# NB600-340).
BerY N, Cruz-Migoni A, Bataille CJ et al. BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions. Elife 2018 Jul 10 [PMID: 29989546] (WB, Human)

Details:
This citation used the HRP version of this antibody.
WB Human
Gelmetti V, DeRosa P, Torosantucci L et al. PINK1 and BECN1 relocalize at mitochondria-associated membranes during mitophagy and promote ER-mitochondria tethering and autophagosome formation. Autophagy. Dec 23 2016 [PMID: 28368777] (ICC/IF, WB, IP, Human) ICC/IF, WB, IP Human
Moutel S, Bery N, Bernard V et al. NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies. Elife. 2016 Jul 19 [PMID: 27434673] (ICC/IF)

Details:
This citation used the HRP version of this antibody.
ICC/IF
Kuroiwa M, Hamada M, Hieda E et al. Muscarinic receptors acting at pre- and post-synaptic sites differentially regulate dopamine/DARPP-32 signaling in striatonigral and striatopallidal neuron Neuropharmacology (IP)

Details:
This publication used the HRP conjugated form of this antibody (Cat# NB600-341).
IP
Chan CT, Paulmurugan R, Gheysens OS et al. Molecular imaging of the efficacy of heat shock protein 90 inhibitors in living subjects Cancer Res

Details:
This publication used the Agarose Immobilized conjugated form of this antibody (Cat# NB600-342).
Somplatzki S, Muhlenhoff M, Kroger A et al. Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells BMC Biotechnol 2017 May 12 [PMID: 28499450] (ICC/IF, Human)

Details:
This reference used the FITC version of NB600-335.
ICC/IF Human
Krishnaswamy A, Yamagata M, Duan X et al. Sidekick 2 directs formation of a retinal circuit that detects differential motion. Nature. 2015 Aug 27 [PMID: 26287463]
Van Humbeeck C, Cornelissen T, Hofkens H et al. Parkin interacts with Ambra1 to induce mitophagy J Neurosci 2011 Jul [PMID: 21753002]

Details:
This citation used the FITC version of this antibody.
Show All 40 Publications.

Review for c-Myc Antibody (NB600-335) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-335:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot c-Myc NB600-335
Enlarge
reviewed by:
Costache Vasilescu
WB Human 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from 293T cells, in lysis buffer
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiondonkey anti-goat IgG-HRP (SCBT)
Secondary Manufacturer Cat##sc-2020
Secondary Concentration1:4000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 20 sec

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose Labeled NB600-342
Biotin Labeled NB600-335B
HRP Labeled NB600-341

Additional Array Products

Bioinformatics Tool for c-Myc Antibody (NB600-335)

Discover related pathways, diseases and genes to c-Myc Antibody (NB600-335). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for c-Myc Antibody (NB600-335)

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Pathways for c-Myc Antibody (NB600-335)

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Research Areas for c-Myc Antibody (NB600-335)

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Blogs on c-Myc. Showing 1-10 of 15 blog posts - Show all blog posts.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.

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Recent Reviews

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1
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Costache Vasilescu
12/18/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MYC