c-Myc Antibody

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Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity ...read more
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ...read more

Product Details

Summary
Reactivity Hu, Mu, All-NA, EcSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

c-Myc Antibody Summary

Immunogen
Goats were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Localization
Cytoplasmic and Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:30000
  • ELISA Coating: 1:100-1:500; Primary: 1:1000-1:30000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
IHC-F reactivity reported in scientific literature (PMID:22411008). ICC/IF reactivity reported in scientific literature (PMID: 28368777). Use in IHC reported in scientific literature (PMID: 30203304).
Control
c-Myc Overexpression Lysate
c-Myc Knockout 293T Cell Lysate
Control Peptide
Reviewed Applications
Read 1 Review rated 5
using
NB600-335 in the following applications:

Publications
Read Publications using
NB600-335 in the following applications:

  • 8 publications
  • IHC
    2 publications
  • 1 publication
  • IP
    8 publications
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:19966808). Human reactivity reported in scientific literature (PMID: 22037249)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Myc Antibody

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N.,... Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-335
Species: Hu, Mu, All-NA, Ec
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IP

Publications for c-Myc Antibody (NB600-335)(45)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
ICC/IF
(8)
IHC
(2)
IHC-Fr
(1)
IP
(8)
WB
(5)
All Applications
Filter By Species
Human
(8)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using NB600-335 Applications Species
Somplatzki S, Muhlenhoff M, Kroger A et al. Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells BMC Biotechnol 2017 May 12 [PMID: 28499450] (ICC/IF, Human)

Details:
This reference used the FITC version of NB600-335.
ICC/IF Human
Brunati M, Perucca S, Han L et al. The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin. Elife 2015 [PMID: 26673890]
Krishnaswamy A, Yamagata M, Duan X et al. Sidekick 2 directs formation of a retinal circuit that detects differential motion. Nature. 2015 Aug 27 [PMID: 26287463]
Kuroiwa M, Hamada M, Hieda E et al. Muscarinic receptors acting at pre- and post-synaptic sites differentially regulate dopamine/DARPP-32 signaling in striatonigral and striatopallidal neuron Neuropharmacology 2012 Dec [PMID: 22971543] (IP)

Details:
This citation used the HRP version of this antibody.
IP
Boldicke T, Somplatzki S, Sergeev G, Mueller PP. Functional inhibition of transitory proteins by intrabody-mediated retention in the endoplasmatic reticulum Methods 2012 Mar [PMID: 22037249] (Human)

Details:
This citation used the FITC version of this antibody.
Human
Imamura R, Wang Y, Kinoshita T et al. Anti-inflammatory activity of PYNOD and its mechanism in humans and mice J Immunol 2010 [PMID: 20393137]

Details:
This citation used the FITC version of this antibody.
Rebholz H, Nishi A, Liebscher S et al. CK2 negatively regulates G{alpha}s signaling PNAS 2009 [PMID: 19666609]

Details:
This citation used the Agarose Immobilized version of this antibody.
Wilkinson DS, Tsai WW, Schumacher MA et al. Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression Mol Cell Biol 2008 Mar [PMID: 18212064]

Details:
This citation used the Agarose Immobilized version of this antibody.
Chan CT, Paulmurugan R, Gheysens OS et al. Molecular imaging of the efficacy of heat shock protein 90 inhibitors in living subjects Cancer Res 2008 Jan [PMID: 18172314]

Details:
This citation used the Agarose Immobilized version of this antibody.
Bateup HS. The Differential Contribution of Striatonigral and Striatopallidal Neurons in Mediating Responses to Therapeutic Agents and Drugs of Abuse: A Dual Role for DARPP-32. Thesis. (IP) IP
Show All 45 Publications.

Review for c-Myc Antibody (NB600-335) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-335:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot c-Myc NB600-335
Enlarge
reviewed by:
Costache Vasilescu
WB Human 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from 293T cells, in lysis buffer
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiondonkey anti-goat IgG-HRP (SCBT)
Secondary Manufacturer Cat##sc-2020
Secondary Concentration1:4000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 20 sec

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose NB600-342
Alexa Fluor 350 NB600-335AF350
Alexa Fluor 405 NB600-335AF405
Alexa Fluor 488 NB600-335AF488
Alexa Fluor 532 NB600-335AF532
Alexa Fluor 594 NB600-335AF594
Alexa Fluor 647 NB600-335AF647
Alexa Fluor 700 NB600-335AF700
Alexa Fluor 750 NB600-335AF750
Biotin NB600-335B
DyLight 350 NB600-335UV
DyLight 405 NB600-335V
DyLight 488 NB600-335G
DyLight 550 NB600-335R
DyLight 594 NB600-335DL594
DyLight 650 NB600-335C
DyLight 680 NB600-335FR
DyLight 755 NB600-335IR
FITC NB600-335F
HRP NB600-341
HRP NB600-335H
Janelia Fluor 549 NB600-335JF549
Janelia Fluor 646 NB600-335JF646

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Blogs on c-Myc. Showing 1-10 of 15 blog posts - Show all blog posts.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.
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Recent Reviews

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5
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4
0
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2
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1
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Costache Vasilescu
12/18/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MYC
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