BIK Antibody - BSA Free

Immunohistochemistry-Paraffin: BIK Antibody [NB100-56109] - Tissue section of human Non-Hodgkin's lymphoma (NHL) stained for Bik expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: BIK Antibody [NB100-56109] - Tissue section of human kidney stained for Bik expression using this antibody at 1:2000. A high level of Bik signal was seen in the distal collecting tubules, the glomeruli are primarily negative. Hematoxylin-eosin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BIK Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BIK Antibody - BSA Free (NB100-56109) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-BIK Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant full-length human Bik protein was used as immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIK
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for BIK Antibody - BSA Free

• Apoptosis inducer NBK
• BCL2-interacting killer (apoptosis-inducing)
• bcl-2-interacting killer
• BIK
• BIP1
• BP4
• NBK
• NBKapoptosis-inducing NBK

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both antiapoptotic (prosurvival) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of antiapoptotic and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Antiapoptotic: Bcl-2, Bcl-xL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-xS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Antiapoptotic members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical antiapoptotic family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of antiapoptotic and proapoptotic proteins determines the susceptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Antiapoptotic proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. This antibody recognizes Bik. Human Bik is a 160 amino acid protein, GenBank no. NP_001188.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BIK Antibody (NB100-56109)(2)

Publications using NB100-56109

• Fu D, Li P, Sheng Q, Lv Z beta-arrestin-2 enhances intestinal epithelial apoptosis in necrotizing enterocolitis Aging 2019-10-14 [PMID: 31612867] (WB, Mouse, Human)
• Suzuki K, Yoshida K, Ueha T et al. Methotrexate upregulates circadian transcriptional factors PAR bZIP to induce apoptosis on rheumatoid arthritis synovial fibroblasts. Arthritis Res. Ther. 2018-03-22 [PMID: 29566767] (WB, Human)

Bioinformatics

• Gene Symbol: BIK
• Entrez:
  • 638(Human)
  • 12124(Mouse)
  • 114496(Rat)