PUMA Antibody

Images

 
Independent Antibodies: Western Blot: PUMA Antibody [NBP1-76639] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 ug of lysates per lane.Antibodies: NBP1-76654 (3 ug/mL), ...read more
Immunocytochemistry/ Immunofluorescence: PUMA Antibody [NBP1-76639] - Analysis of PUMA in K562 cells with PUMA antibody at 20 ug/ml.
Immunohistochemistry-Paraffin: PUMA Antibody [NBP1-76639] - Human breast carcinoma with PUMA antibody at 10 ug/mL. Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 ug/mL.
Western Blot: PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: PUMA Antibody [NBP1-76639] - K562 cells.
Immunohistochemistry: PUMA Antibody [NBP1-76639] - Analysis of PUMA in human breast tissue with PUMA antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

PUMA Antibody Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a. The sequence is identical between a and b forms of the PUMA proteins. The immunogen is located within the first 50 amino acids of PUMA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BBC3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Publications
Read Publications using
NBP1-76639 in the following applications:

  • 1 publication
  • WB
    4 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 16399862). Mouse (79%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PUMA Antibody

  • BCL2 binding component 3
  • bcl-2-binding component 3
  • JFY1
  • JFY-1
  • p53 up-regulated modulator of apoptosis
  • PUMAFLJ42994

Background

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PUMA Antibody (NBP1-76639)(5)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(3)
Rat
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP1-76639 Applications Species
Choi SH, Koh DI, Cho SY et al. Temporal and differential regulation of KAISO-controlled transcription by phosphorylated and acetylated p53 highlights a crucial regulatory role of apoptosis J. Biol. Chem. Jul 11 2019 [PMID: 31296660] (WB, Human) WB Human
Long JS, Schoonen PM, Graczyk D et al. p73 engages A2B receptor signalling to prime cancer cells to chemotherapy-induced death Oncogene. 2015 Feb 09 [PMID: 25659586] (WB, Human) WB Human
Fandy TE, Ross DD, Gore SD, Srivastava RK. Flavopiridol synergizes TRAIL cytotoxicity by downregulation of FLIPL. Cancer Chemother Pharmacol. 2007 Aug [PMID: 17187253] (WB)

Details:
Puma alpha NT (IMG-458-1). WB: MM1S myeloma cell line treated with flavopiridol, Fig 4A.
WB
Toth A, Jeffers JR, Nickson P et al. Targeted deletion of Puma attenuates cardiomyocyte death and improves cardiac function during ischemia-reperfusion. Am J Physiol Heart Circ Physiol. 2006 Jul [PMID: 16399862] (WB, Rat)

Details:
WB: Fig 1B (primary rat cardiomyocytes exposed to hypoxia, Puma is identified as a 22 kDa band), Fig 2B (primary rat cardiomyoctyes infected with GFP-conjugated Puma-alpha adenoviruses). Note: The specificity of the PUMA IMG-458 antibody was Puma-alpha ad
WB Rat
Karst AM, Dai DL, Martinka M, Li G. PUMA expression is significantly reduced in human cutaneous melanomas. Oncogene. 2005 Feb 3 [PMID: 15690057] (IHC-P, Human)

Details:
Puma (IMG-458). IHC (paraffin): Human dysplastic nevi, primary melanoma, and metastatic melanoma tumor tissues, Fig 1.
IHC-P Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PUMA Antibody (NBP1-76639) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for PUMA Antibody (NBP1-76639)

Discover related pathways, diseases and genes to PUMA Antibody (NBP1-76639). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PUMA Antibody (NBP1-76639)

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Blogs on PUMA.

A link between Autophagy and Apoptosis: Chat with First Author Brent E. Fitzwalter
By Christina Towers, PhD. Autophagy is a cellular recycling process and most often a pro-survival mechanism that regulates cellular homeostasis. On the contrary, apoptosis is an extensively conserved and elaborate pr...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Bioinformatics

Gene Symbol BBC3
Uniprot