Independent Antibodies: Western Blot: PUMA Antibody [NBP1-76639] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 ug of lysates per lane.Antibodies: NBP1-76654 (3 ug/mL), ...read more
Immunocytochemistry/ Immunofluorescence: PUMA Antibody [NBP1-76639] - Analysis of PUMA in K562 cells with PUMA antibody at 20 ug/ml.
Immunohistochemistry-Paraffin: PUMA Antibody [NBP1-76639] - Human breast carcinoma with PUMA antibody at 10 ug/mL. Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 ug/mL.
Western Blot: PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 ug/ml.
Antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a. The sequence is identical between a and b forms of the PUMA proteins. The immunogen is located within the first 50 amino acids of PUMA.
Peptide affinity purified
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23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-76639 in the following applications:
Rat reactivity reported in scientific literature (PMID: 16399862). Mouse (79%).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for PUMA Antibody
BCL2 binding component 3
bcl-2-binding component 3
p53 up-regulated modulator of apoptosis
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Altered expression of BCL2 in cancer Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease. For example, too little cell death can promote cell overgrowth a... Read full blog post.
Understanding Noxa Regulation of Apoptosis Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb... Read full blog post.
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