Bad Antibody

Images

 
Western Blot: Bad Antibody [NB100-56080] - Analysis of Bad in Daoy whole cell lysate using anti-Bad antibody. Image from verified customer review.
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Bad Antibody [NB100-56080] - Immunofluorescence microscopy of BAD using NB100-56080 at 1:2000. Du145 human prostate carcinoma cells were cultured ...read more
Immunohistochemistry-Paraffin: Bad Antibody [NB100-56080] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using 1:2000 conc. of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

Bad Antibody Summary

Immunogen
A full length recombinant protein corresponding to human Bad was used as immunogen.
Specificity
Bad
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAD
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunohistochemistry 1:2000
  • Immunohistochemistry-Paraffin 1:2000
  • Immunoprecipitation 1:50-1:200
Control
Bad Overexpression Lysate
Bad Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-56080 in the following applications:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Bad Antibody

  • Bad
  • BBC2
  • BBC6
  • bcl2 antagonist of cell death
  • BCL2-antagonist of cell death protein
  • BCL2-associated agonist of cell death
  • Bcl-2-binding component 6
  • BCL2-binding component 6
  • BCL2-binding protein
  • BCL2L8
  • Bcl2-L-8
  • BCL2L8bcl2-L-8
  • Bcl-2-like protein 8
  • BCL-X/BCL-2 binding protein
  • Bcl-XL/Bcl-2-associated death promoter

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. Human Bad is a 168 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Bad Antibody (NB100-56080) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56080:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Bad NB100-56080
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5
reviewed by:
Anonymous
WB Human 07/14/2016
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from Daoy cells
SpeciesHuman

Blocking

Blocking Details5% Milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:2000 in 5% milk PBST overnight at 4 degrees C

Secondary Antibody

Secondary DescriptionDonkey anti Rabbit IgG HRP
Secondary Manufacturer Cat#Southern Biotech 6440-05
Secondary Concentration1:5000

Details

Detection NotesAdvansta WesternBright ECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bad Antibody (NB100-56080) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Bad Antibody (NB100-56080)

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Diseases for Bad Antibody (NB100-56080)

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Pathways for Bad Antibody (NB100-56080)

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Research Areas for Bad Antibody (NB100-56080)

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Blogs on Bad.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

AKT Antibody Assays: A Complex Area with an Easy Solution
We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/14/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol BAD
Entrez