Fas/TNFRSF6/CD95 Antibody

Images

 
Western Blot: Fas/TNFRSF6/CD95 Antibody [NB120-13550] - FAS Western blot for whole cell lysate from mouse testis.
Immunohistochemistry-Paraffin: Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Staining of human hepatoma tissue with Fas polyclonal antibody.
Western Blot: Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of mouse testis tissue extractm probed with Fas polyclonal antibody.
Western Blot: Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Lane 1: Rat Brain Tissue Extract. Lane 2: Mouse Brain Tissue Extract.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Fas/TNFRSF6/CD95 Antibody Summary

Immunogen
Synthetic peptide: ESLKLRRRVHETDKNC conjugated to KLH, corresponding to amino acids 29-44 of Mouse CD95.
Localization
Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6).
Specificity
CD95
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FAS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
Flow Cytometry Use at an assay dependent dilution. WB: Use at an assay dependent dilution. For colourimetric Western blotting use at 4ug/ml, however when detecting with ECL use at a concentration of 0.5ug/ml. Predicted molecular weight: 44 kDa. In samples from hum. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28340583).
Positive Control
HeLa Lysate (NB800-PC1)
Fas/TNFRSF6/CD95 Lysate (NBL1-10598)
Reviewed Applications
Read 1 Review rated 5
using
NB120-13550 in the following applications:

Publications
Read Publications using
NB120-13550 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fas/TNFRSF6/CD95 Antibody

  • Apo-1 antigen
  • Apo-1
  • apoptosis antigen 1
  • Apoptosis-mediating surface antigen FAS
  • APT1
  • APT1FASTM
  • CD95 antigen
  • CD95
  • CD95ALPS1A
  • Fas (TNF receptor superfamily, member 6)
  • Fas AMA
  • Fas antigen
  • Fas
  • FAS1
  • FASLG receptor
  • TNFRSF6
  • TNFRSF6member 6
  • tumor necrosis factor receptor superfamily member 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Fas/TNFRSF6/CD95 Antibody (NB120-13550)(3)

Review for Fas/TNFRSF6/CD95 Antibody (NB120-13550) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB120-13550:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Fas/TNFRSF6/CD95 NB120-13550
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reviewed by:
Ranveer Singh
WB Mouse 07/24/2014
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Summary

ApplicationWestern Blot
Sample TestedLysate from testis of six week old mouse
SpeciesMouse

Blocking

Blocking Details5% BSA in TBST for 1 h

Primary Anitbody

Dilution Ratio1:1000, Overnight at 4 degree in TBST on a rocker

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG HRP (Biorad)
Secondary Manufacturer Cat#170-5046
Secondary Concentration1:20,000 in 5% BSA

Details

Detection NotesECL reagent was used. Exposure for 5 seconds

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fas/TNFRSF6/CD95 Antibody (NB120-13550) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Fas/TNFRSF6/CD95 Antibody (NB120-13550)

Discover related pathways, diseases and genes to Fas/TNFRSF6/CD95 Antibody (NB120-13550). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Fas/TNFRSF6/CD95 Antibody (NB120-13550)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ranveer Singh
07/24/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol FAS
Entrez
Uniprot