Caspase-9 Antibody


Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem more
Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Increased the expression of Caspase-9 in 12-week-old Atg7deltapan mice. Caspase-9 more
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.
Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis and altered inflammation in double-deficient Atg7deltapan-Rip3-/- mice. (c) Depletion of Rip3 and pancreatic Atg7 (Atg7deltapan-Rip3d/d) more
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for Active/Cleaved Caspase-9 expression at 1:2000. A and B. The pattern of Caspase-9 staining may vary between more
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a dog brain 2 hr after more
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis Human Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a patient 24 hr after head trauma. more

Product Details

Reactivity Hu, Mu, Rt, Ca, MaSpecies Glossary
Applications WB, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Order Details

Caspase-9 Antibody Summary

Human Caspase 9 recombinant protein catalytic subunits (NP_001220).
This antibody detects both the pro- and active-Caspase 9 forms. In western blots, the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.
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  • Flow (Intracellular)
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Assay Dependent
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot
Application Notes
Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active/cleaved Caspase-9 staining may also be seen in the mitochondria. 2. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation. Use in WB reported in scientific literature (PMID: 24184144). Use in ICC/IF reported in scientific literature (PMID: 22223358).
Jurkat Staurosporine Treated / Untreated Cell Lysate
Read Publications using
NB100-56118 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Whole antisera
0.05% Sodium Azide

Alternate Names for Caspase-9 Antibody

  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • Caspase-9
  • EC
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease


Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-9 Antibody (NB100-56118)(20)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 5 applications: Flow-IC, ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB100-56118 Applications Species
Gao K, Niu J, Dang X Wnt-3a improves functional recovery through autophagy activation via inhibiting the mTOR signaling pathway after spinal cord injury Neurosci. Lett. Aug 17 2020 [PMID: 32818590] (WB, Rat) WB Rat
Xie L, Xia L, Klaiber U et al. Effects of neoadjuvant FOLFIRONOX and gemcitabine-based chemotherapy on cancer cell survival and death in patients with pancreatic ductal adenocarcinoma Oncotarget Dec 31 2019 [PMID: 31921387] (ICC/IF, Human) ICC/IF Human
Zhao L, Zhu Z, Yao C et al. VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2 Cell Cycle 2017 Nov 23 [PMID: 29169284] (WB, Mouse) WB Mouse
Zhou X, Xie L, Xia L et al. RIP3 attenuates the pancreatic damage induced by deletion of ATG7 Cell Death and Disease Jul 13 2017 [PMID: 28703808] (ICC/IF, Mouse) ICC/IF Mouse
Kato S, Kato M, Kusano T, Nishino T. New Strategy That Delays Progression of Amyotrophic Lateral Sclerosis in G1H-G93A Transgenic Mice: Oral Administration of Xanthine Oxidoreductase Inhibitors That Are Not Substrates for the Purine Salvage Pathway. J. Neuropathol. Exp. Neurol. Nov 4 2016 [PMID: 27815397] (IHC-P, Mouse) IHC-P Mouse
Sundberg JP, Silva KA, King LE, Pratt CH. Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening. Methods Mol. Biol. May 6 2016 [PMID: 27150092] (IHC, Mouse) IHC Mouse
Erdogan H, Tuncdemir M, Kelten B et al. Posterior Cerebral Artery Insufficiency in Pediatric Moyamoya Disease J Korean Neurosurg Soc. 2015 Jun 01 [PMID: 26180614] (IHC-P, Rat) IHC-P Rat
Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID: 24804954] (Flow-IC, Human)

Jurkat, Fig 9.
Flow-IC Human
Potter CS, Wang Z, Silva KA et al. Chronic Proliferative Dermatitis in Sharpin Null Mice: Development of an Autoinflammatory Disease in the Absence of B and T Lymphocytes and IL4/IL13 Signaling. PLoS ONE 2014 Jan 27 [PMID: 24465642] (IHC-P, Mouse) IHC-P Mouse
Topcu I, Vatansever S, Bayram E et al. The effects of lornoxicam on neuroprotection following diffuse traumatic brain injury in rats. Turk Neurosurg 2013 [PMID: 24310460] (IHC-P, Rat) IHC-P Rat
Show All 20 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-9 Antibody (NB100-56118). (Showing 1 - 1 of 1 FAQs).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on Caspase-9.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Gene Symbol CASP9