Caspase-9 Antibody - BSA Free

Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem mice as evidenced by antibody detection of cleaved CASPASE 9 (T) when compared to Sharpincpdm mutants (R) or WT mice (Q,S). . Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085666) licensed under a CC-BY license.

Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Increased the expression of Caspase-9 in 12-week-old Atg7deltapan mice. Caspase-9 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (green) (1/1000, scale bar=50?um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.

Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis and altered inflammation in double-deficient Atg7deltapan-Rip3-/- mice. (c) Depletion of Rip3 and pancreatic Atg7 (Atg7deltapan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation and representative IF microphotographs of Atg7deltapan (n=5) and Atg7deltapan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (red) (1/1000).. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for Active/Cleaved Caspase-9 expression at 1:2000. A and B. The pattern of Caspase-9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a dog brain 2 hr after reperfusion injury. B) ection from a dog brain sham control (brain surgery but no injury). Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis Human Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a patient 24 hr after head trauma. B) Control: section from a patient with no known neurological disease or head injury. Hematoxylin-eosin counterstain.

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis & altered inflammation in double-deficient Atg7 delta pan-Rip3−/− mice. (a) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic caspase-3 in 12-week-old mice. Caspase-3 quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-3 (red) (anti-active caspase-3 ab2302, 1/50, scale bar=50 μm). (b) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic Bax in 12-week-old mice. Bax quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-3 (red) (anti-Bax (sc-526, 1/50). (c) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-9 (red) (anti-caspase-9 NB100-56118, 1/1000). (d) Reduced infiltration inflammation of macrophages (F4/80, NBP2-12506, 1/75) & early T-lymphocytes (MPO, ab9535, 1/50) in double-deficient Atg7 delta pan-Rip3d/d (n=10) mice compared with pancreatic Atg7 delta pan (n=7). Data are mean±S.E.M. for the numbers of animals as indicated in the graph, *P<0.05, **P<0.01, NS = no significance Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28703808), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ca, Ma
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Caspase-9 Antibody - BSA Free Summary

• Immunogen: Human Caspase 9 recombinant protein catalytic subunits (NP_001220).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP9
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 22223358)
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Assay Dependent
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot reported in scientific literature (PMID 24184144)
• Application Notes: Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active/cleaved Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation: the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.
• Control:

  Jurkat Staurosporine Treated / Untreated Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Caspase-9 Antibody - BSA Free

• APAF-3APAF3
• apoptotic protease activating factor 3
• Apoptotic protease Mch-6
• Apoptotic protease-activating factor 3
• CASP9
• CASP-9
• caspase 9, apoptosis-related cysteine peptidase
• Caspase9
• Caspase-9
• EC 3.4.22.62
• ICE-LAP6CASPASE-9c
• ICE-like apoptotic protease 6
• MCH6apoptosis-related cysteine protease

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-9 Antibody (NB100-56118)(28)

Publications using NB100-56118

• Srivastava S, Gajwani P, Jousma J et al. Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism Nature communications 2023-07-19 [PMID: 37468519] (WB, Human)
  
  Details:
  Dilutions: 1:1000
• Feng Y, Yang L, Ma X et al. Photobiomodulation treatment inhibits neurotoxic astrocytic polarization and protects neurons in in vitro and in vivo stroke models Neurochemistry international 2022-12-17 [PMID: 36539162] (IHC-Fr, Mouse)
• Eskiler GG, Turna O, Ozkan AD et al. The response of the canine mammary simple carcinoma and carcinosarcoma cells to 5-aminolaevulinic acid-based photodynamic therapy: An in vitro study Journal of photochemistry and photobiology. B, Biology 2022-07-05 [PMID: 35810598] (WB, Canine)
• Li J, Camirand A, Zakikhani M et al. Parathyroid hormone related protein inhibition blocks triple negative breast cancer expansion in bone through epithelial to mesenchymal transition reversal JBMR Plus 2022-06-20 [PMID: 35720668]
• Espinosa-Jeffrey A, Arrazola RA, Chu B et al. Trophic factors intervention regenerates the nestin-expressing cell population in a model of perinatal excitotoxicity: Implications for perinatal brain injury and prematurity Integrative Molecular Medicine 2016-06-25 [PMID: 35558521] (Western Blot, Human)
• Doke T, Huang S, Qiu C et al. Genome-wide association studies identify the role of caspase-9 in kidney disease Science Advances 2021-11-05 [PMID: 34739325] (Western Blot, Human)
• Yan Huo, Abudureheman Mijiti, Ruonan Cai, Zhaohua Gao, Maierpu Aini, Abudukadier Mijiti, Zhaoling Wang, Rui Qie Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice. Human & experimental toxicology 2022-03-07 [PMID: 34610774]
• Gao K, Niu J, Dang X Wnt-3a improves functional recovery through autophagy activation via inhibiting the mTOR signaling pathway after spinal cord injury Neurosci. Lett. 2020-08-17 [PMID: 32818590] (WB, Rat)
• Xie L, Xia L, Klaiber U et al. Effects of neoadjuvant FOLFIRONOX and gemcitabine-based chemotherapy on cancer cell survival and death in patients with pancreatic ductal adenocarcinoma Oncotarget 2019-12-31 [PMID: 31921387] (ICC/IF, Human)
• Jaworski S, Strojny B, Sawosz E et al. Degradation of Mitochondria and Oxidative Stress as the Main Mechanism of Toxicity of Pristine Graphene on U87 Glioblastoma Cells and Tumors and HS-5 Cells Int J Mol Sci 2019-02-02 [PMID: 30717385] (Western Blot, Human)
• Zhao L, Zhu Z, Yao C et al. VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2 Cell Cycle 2017-11-23 [PMID: 29169284] (WB, Mouse)
• Zhou X, Xie L, Xia L et al. RIP3 attenuates the pancreatic damage induced by deletion of ATG7 Cell Death and Disease 2017-07-13 [PMID: 28703808] (ICC/IF, Mouse)
• Zhou X, Xie L, Bergmann F et al. The bile acid receptor FXR attenuates acinar cell autophagy in chronic pancreatitis. Cell Death Discov. 2017-06-29 [PMID: 28660075] (Western Blot, Human)
• Kato S, Kato M, Kusano T, Nishino T. New Strategy That Delays Progression of Amyotrophic Lateral Sclerosis in G1H-G93A Transgenic Mice: Oral Administration of Xanthine Oxidoreductase Inhibitors That Are Not Substrates for the Purine Salvage Pathway. J. Neuropathol. Exp. Neurol. 2016-11-04 [PMID: 27815397] (IHC-P, Mouse)
• Sundberg JP, Silva KA, King LE, Pratt CH. Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening. Methods Mol. Biol. 2016-05-06 [PMID: 27150092] (IF/IHC, Mouse)
• Erdogan H, Tuncdemir M, Kelten B et al. Posterior Cerebral Artery Insufficiency in Pediatric Moyamoya Disease J Korean Neurosurg Soc. 2015-06-01 [PMID: 26180614] (IHC-P, Rat)
• Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014-05-07 [PMID: 24804954] (Flow Cytometry Control, Human)
  
  Details:
  Mouse splenocytes, human PBMC, Jurkat, EL4: Fig 5. GADPH was detected at ~36 kDa.
• Potter CS, Wang Z, Silva KA et al. Chronic Proliferative Dermatitis in Sharpin Null Mice: Development of an Autoinflammatory Disease in the Absence of B and T Lymphocytes and IL4/IL13 Signaling. PLoS ONE 2014-01-27 [PMID: 24465642] (IHC-P, Mouse)
• Topcu I, Vatansever S, Bayram E et al. The effects of lornoxicam on neuroprotection following diffuse traumatic brain injury in rats. Turk Neurosurg 2013-01-01 [PMID: 24310460] (IHC-P, Rat)
• Ichihara A, Wang Z, Jinnin M et al. Upregulation of miR-18a-5p contributes to epidermal necrolysis in severe drug eruptions. J Allergy Clin Immunol 2013-11-01 [PMID: 24184144] (WB, Human)
  
  Details:
  Primary human epidermal keratinocytes, Fig 4B.
• Herold C, Rennekampff HO, Ohm L, Strauss S, Linkner J, Reimers K, Allmeling C, Vaske B, Vogt PM. Apoptosis in extracorporeal preserved inguinal fat flaps of the rat. Apoptosis;17(4):400-9. 2012-04-01 [PMID: 22223358] (IF/IHC, ICC/IF, Rat)
• Zhang X, Chen W, De Paiva C et al. Interferon exacerbates dry eye-induced apoptosis in conjunctiva through dual apoptotic pathways Invest Ophthalmol Vis Sci 2011-08-09 [PMID: 21474767]
• Liu G, Keeler BE, Zhukareva V, Houle JD. Cycling exercise affects the expression of apoptosis-associated microRNAs after spinal cord injury in rats. Exp Neurol. 2010-11-01 [PMID: 20816819]
• Price CD, Yang Z, Karlnoski R et al. Effect of continuous infusion of asialoerythropoietin on short-term changes in infarct volume, penumbra apoptosis and behaviour following middle cerebral artery occlusion in rats. Clin Exp Pharmacol Physiol. 2010-02-01 [PMID: 19650797]
• Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004-10-01 [PMID: 15380478] (WB, Rat, Canine, Mouse)
  
  Details:
  Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne
• Hermel E, Gafni J, Propp SS et al. Specific caspase interactions and amplification are involved in selective neuronal vulnerability in Huntington's disease. Cell Death Differ. 2004-04-01 [PMID: 14713958] (IHC-P)
  
  Details:
  Antibodies cited: 1. Caspase-8 (IMG-5703): IP/WB, Fig 2 (293T transfected with huntingtin constructs), IHC (P), Fig 5i & j (mouse cortex and striatum) 2. Caspase-9 (IMG-5705): IHC (P), Fig 5a & b (mouse striatum)
• Cowan CM, Thai J, Krajewski S et al. Caspases 3 and 9 send a pro-apoptotic signal from synapse to cell body in olfactory receptor neurons. J Neurosci. 2001-09-15 [PMID: 11549720]
• Krajewski S, Krajewska M, Ellerby LM et al. Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia. Proc Natl Acad Sci U S A. 1999-05-11 [PMID: 10318956] (WB, Human)

Review for Caspase-9 Antibody (NB100-56118) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Chi Li IHC-P Mouse 01/08/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Transplanted lung tumor section
• Species: Mouse
• Lot: AR19A-080906-05

FAQs for Caspase-9 Antibody (NB100-56118). (Showing 1 - 1 of 1 FAQs).

1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
   • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Bioinformatics

• Gene Symbol: CASP9
• Entrez:
  • 842(Human)
  • 58918(Rat)
• Uniprot:
  • P55211()