Caspase-9 Antibody


Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis Human Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A. Section from a patient 24 hr after head trauma. more
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for Active/Cleaved Caspase-9 expression at 1:2000. A and B. The pattern of Caspase-9 staining may vary between more
Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A. Section from a dog brain 2 hr after more

Product Details

Reactivity Hu, Mu, Rt, Ca, GeSpecies Glossary
Applications Flow-IC, IHC, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Caspase-9 Antibody Summary

Recombinant catalytically active human Caspase-9 protein was used as immunogen (NP_001220).
This polyclonal antisera preferentially recognizes active/cleaved caspase-9. Whereas the antisera has a strong preference for active/cleaved caspase-9, in some cell or tissue systems or techniques the antisera may also recognize the proform of caspase-9. On western blots, the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. ntermediate caspase-9 cleavage forms may also be seen at ~21 kDa.
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
This antibody is useful for immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen. Use in Flow Intracellular reported in scientific literature (PMID 24804954)
Reviewed Applications
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NB100-56118 in the following application:

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NB100-56118 in the following applications:

Reactivity Notes

Dog, Gerbil, Human, Mouse, Rat Not yet tested in other species.

Alternate Names for Caspase-9 Antibody

  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • Cas9
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • caspase-9
  • EC
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease


Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: WB, Simple Western, IP
Species: Hu, Mu, Rt, Ca, Ge
Applications: WB, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu
Applications: WB, ICC/IF, IHC-P
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Publications for Caspase-9 Antibody (NB100-56118)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 5 applications: Flow-IC, ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-56118 Applications Species
Sundberg JP, Silva KA, King LE, Pratt CH. Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening. Methods Mol. Biol. May 6 2016 12:00AM [PMID:27150092] (IHC, Mouse) IHC Mouse
Erdogan H, Tuncdemir M, Kelten B et al. Posterior Cerebral Artery Insufficiency in Pediatric Moyamoya Disease J Korean Neurosurg Soc. 2015 Jun 01 [PMID:26180614] (IHC-P, Rat) IHC-P Rat
Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID:24804954] (Flow-IC, Human) Flow-IC Human
Potter CS, Wang Z, Silva KA et al. Chronic Proliferative Dermatitis in Sharpin Null Mice: Development of an Autoinflammatory Disease in the Absence of B and T Lymphocytes and IL4/IL13 Signaling. PLoS ONE 2014 Jan 27 [PMID:24465642] (IHC-P, Mouse) IHC-P Mouse
Topcu I, Vatansever S, Bayram E et al. The effects of lornoxicam on neuroprotection following diffuse traumatic brain injury in rats. Turk Neurosurg 2013 [PMID:24310460] (IHC-P, Rat) IHC-P Rat
Ichihara A, Wang Z, Jinnin M et al. Upregulation of miR-18a-5p contributes to epidermal necrolysis in severe drug eruptions. J Allergy Clin Immunol 2013 Nov 1 [PMID:24184144] (WB, Human) WB Human
Herold C, Rennekampff HO, Ohm L, Strauss S, Linkner J, Reimers K, Allmeling C, Vaske B, Vogt PM. Apoptosis in extracorporeal preserved inguinal fat flaps of the rat. Apoptosis;17(4):400-9. 2012 Apr. [PMID:22223358] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Zhang X, Chen W, De Paiva CS et al. Interferon-{gamma} Exacerbates Dry Eye Induced Apoptosis in Conjunctiva via Dual Apoptotic Pathways. Invest Ophthalmol Vis Sci;52(9):6279-85. 2011 Aug 9. [PMID:21474767]
Liu G, Keeler BE, Zhukareva V, Houle JD. Cycling exercise affects the expression of apoptosis-associated microRNAs after spinal cord injury in rats. Exp Neurol. 2010 Nov [PMID:20816819]
Price CD, Yang Z, Karlnoski R et al. Effect of continuous infusion of asialoerythropoietin on short-term changes in infarct volume, penumbra apoptosis and behaviour following middle cerebral artery occlusion in rats. Clin Exp Pharmacol Physiol. 2010 Feb [PMID:19650797]
Show All 14 Publications.

Reviews for Caspase-9 Antibody (NB100-56118) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56118:
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Images Ratings Applications Species Date Details
 Caspase-9 NB100-56118
reviewed by:
Mouse 01/08/2015


Sample TestedTransplanted lung tumor section

Product General Protocols

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FAQs for Caspase-9 Antibody (NB100-56118). (Showing 1 - 1 of 1 FAQs).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

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Blogs on Caspase-9.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Gene Symbol CASP9

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