beta-Actin RNAi



Product Details

Reactivity HuSpecies Glossary
Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Order Details

beta-Actin RNAi Summary

Homo sapiens actin, beta (ACTB), mRNA

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.


Reviewed Applications
Read 1 Review rated 5
H00000060-R01 in the following application:


This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for beta-Actin RNAi

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • Beta-actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1


Position of the Chimera RNAi. The related RNAi products listed were designed from different accesion number but sharing the same RNAi sequence. Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene.  The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.  Chimera RNAi has many advantages over the conventional siRNAs.  First, it has been demonstrated to have reliable knock-down for over 10,000 human genes.  Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence.  Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected.  Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IP, ChIP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
Species: Hu, Mu
Applications: WB, Simple Western, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
Species: Hu, Mu, Po
Applications: WB, Simple Western, ICC/IF
Species: Hu, Mu, Po, Bv, Ca, Op, Pm
Applications: WB, IHC-P
Species: Hu
Applications: WB, Simple Western
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ye
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Multi
Applications: WB, ICC
Species: Hu
Applications: WB
Species: Hu
Applications: PAGE
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for beta-Actin RNAi (H00000060-R01) (0)

There are no publications for beta-Actin RNAi (H00000060-R01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for beta-Actin RNAi (H00000060-R01) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00000060-R01:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Western Blot beta-Actin H00000060-R01
reviewed by:
WB Human 12/09/2014


ApplicationWestern Blot
Sample TestedWhole cell lysate from HeLa cells


Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio2000 times diluted in PBS, incubated for 2 hours at room temperature


Detection NotesCaptured using X-rays (1min exposure) with Phototope-HRP Western Blot Detection System

FAQs for beta-Actin RNAi (H00000060-R01). (Showing 1 - 4 of 4 FAQ).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.

Additional beta-Actin RNAi Products

Related Products by Gene

Bioinformatics Tool for beta-Actin RNAi (H00000060-R01)

Discover related pathways, diseases and genes to beta-Actin RNAi (H00000060-R01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta-Actin RNAi (H00000060-R01)

Discover more about diseases related to beta-Actin RNAi (H00000060-R01).

Pathways for beta-Actin RNAi (H00000060-R01)

View related products by pathway.

PTMs for beta-Actin RNAi (H00000060-R01)

Learn more about PTMs related to beta-Actin RNAi (H00000060-R01).

Research Areas for beta-Actin RNAi (H00000060-R01)

Find related products by research area.

Blogs on beta-Actin.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol ACTB

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on H00000060-R01 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought