ATP7b Antibody [Biotin]


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Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Please see the vial label for concentration. If unlisted please contact technical services.

ATP7b Antibody [Biotin] Summary

A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b. [UniProt# P35670]
Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140 kDa is located in mitochondria.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
Biotin (A=N/A, E=N/A)
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


Application Notes
This ATP7b antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. By WB, this antibody recognizes a band at 165 kDa, representing ATP7b and also recognizes a faint non-specific band at ~195 kDa. In IHC-P, cytoplasmic and golgi staining was observed in mouse liver tissue (1:100 dilution) and human ovarian carcinoma cells (1:500 dilution). However, please note we also tested this antibody with IHC-P in mouse intestines and kidney and observed unexpected strong nuclear signal with lighter cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
165 kDa
Readout System

Reactivity Notes

Human, rat and mouse.

Alternate Names for ATP7b Antibody [Biotin]

  • ATPase, Cu(2+)- transporting, beta polypeptide
  • ATPase, Cu++ transporting, beta polypeptide
  • Copper pump 2
  • copper-transporting ATPase 2
  • EC 3.6.3
  • EC
  • PWD
  • WC1
  • WD
  • Wilson disease-associated protein
  • WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)


ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

DyLight 488 Labeled NB100-360G
DyLight 550 Labeled NB100-360R
DyLight 650 Labeled NB100-360C
HRP Labeled NB100-360H

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Contact Information

Product PDFs


Gene Symbol ATP7B

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