ATP7b Antibodies
We offer ATP7b Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each ATP7b Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ATP7b Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the ATP7b Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ATP7b polyclonal antibodies and browse our ATP7b monoclonal antibody catalog.
Alternate Names for ATP7b Antibodies
anti-ATP7b antibody, anti-ATPase, Cu(2+)- transporting, beta polypeptide antibody, anti-ATPase, Cu++ transporting, beta polypeptide antibody, anti-Copper pump 2 antibody, anti-copper-transporting ATPase 2 antibody, anti-EC 3.6.3 antibody, anti-EC 3.6.3.4 antibody, anti-PWD antibody, anti-WC1 antibody, anti-WD antibody, anti-Wilson disease-associated protein antibody, anti-WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease) antibody
10 Results for "atp7b" in Primary Antibodies
![Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Western-Blot-NB100-360-img0003.jpg "Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.")
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-360] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ATP7b [NB100-360] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunocytochemistry-Immunofluorescence-NB100-360-img0005.jpg)
![Immunohistochemistry: ATP7b Antibody [NB100-360] - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-NB100-360-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-361] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATP7b Antibody NB100-361 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunocytochemistry-Immunofluorescence-NB100-361-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-361] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATP7b Antibody NB100-361 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ATP7b Antibody [NB100-361] - Effects & mechanism of action of CASIN on melphalan-resistant MM cells. (A) CASIN reduces Cdc42 activity (Cdc42-GTP) but not Rac1 activity (Rac1-GTP). Melphalan-sensitive MM cells (S) were treated with Vehicle or CASIN (5 μM) for 8 h. Cdc42 & Rac1 activities were measured using pull-down assay. (B) CASIN (5 μM) inhibits proliferation of both S & melphalan-resistant (LR5) MM cells. **P < 0.01 vs. S-Vehicle; ##P < 0.01 vs. LR5-Vehicle. (C) CASIN (5 μM) preferentially causes apoptosis of melphalan-resistant MM cells. S & LR5 cells were treated with or without CASIN or melphalan (Mel, 25 μM) or both for 2 days. Annexin V+ cells were analyzed using flow cytometry. **P < 0.01. (D–F) Mechanism of action of CASIN on melphalan-resistant MM cells. (D) Cdc42 activity & expression are increased in LR5 cells compared to that in S cells. Cdc42 activity was measured using pull-down assay. beta -Actin was used as loading control. Vertical lines indicate the gel lanes being switched in position from the original blots. (E) CASIN (5 μM) decreases melphalan-induced FANCD2 mono-ubiquitination (Ub-FANCD2) in LR5 but not in S cells. S & LR5 cells were treated with or without CASIN, Mel (25 μM), or both for 16 h. FANCD2 was detected using western blotting. beta -Actin was used as loading control. (F) CASIN sensitizes melphalan-resistant but not -sensitive MM cells to melphalan-induced DNA damage. gamma H2A histone family member X-positive ( gamma H2AX+) cells were detected using flow cytometry. **P < 0.01. Error bars represent means ± SD of triplicates & data are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31632904), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-361_rabbit-polyclonal-atp7b-antibody-310202415535181.jpg)
![Western Blot: ATP7b Antibody (S62-29) [NBP2-59375] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Western-Blot-NBP2-59375-img0005.jpg "Western Blot: ATP7b Antibody (S62-29) [NBP2-59375] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Tissue: NIH 3T3 (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Copper Transporting ATPase 2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0007.jpg)
![Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] - Image of ATP7b Antibody (36D12) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.](http://images.novusbio.com/fullsize/nbp3-26488_rabbit-atp7b-mab-36d12-262202414471517.jpg "Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] - Image of ATP7b Antibody (36D12) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.")
![Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] - Image of ATP7b Antibody (36D12) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.](http://images.novusbio.com/fullsize/nbp3-26488_rabbit-atp7b-mab-36d12-262202415253317.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (36D12) [NBP3-26488] - Staining of HepG2 with ATP7b Antibody (36D12) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26488_rabbit-atp7b-mab-36d12-28220241323633.jpg)
![Flow Cytometry: ATP7b Antibody (36D12) [NBP3-26488] - Overlay Peak curve showing HepG2 cells stained with ATP7b Antibody (36D12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26488_rabbit-atp7b-mab-36d12-282202414183628.jpg)
![Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-Paraffin-NBP2-48523-img0004.jpg "Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-Paraffin-NBP2-48523-img0002.jpg)
![Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-Paraffin-NBP2-48523-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Testis shows moderate granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-Paraffin-NBP2-48523-img0005.jpg)
![Western Blot: ATP7b Antibody (3E10) [H00000540-M01] - ATP7B monoclonal antibody (M01), clone 3E10. Analysis of ATP7B expression in human colon.](http://images.novusbio.com/fullsize/ATP7b-Antibody-3E10-Western-Blot-H00000540-M01-img0003.jpg "Western Blot: ATP7b Antibody (3E10) [H00000540-M01] - ATP7B monoclonal antibody (M01), clone 3E10. Analysis of ATP7B expression in human colon.")
![ELISA: ATP7b Antibody (3E10) [H00000540-M01] - ATP7b Antibody (3E10) [H00000540-M01] - Detection limit for recombinant GST tagged ATP7B is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATP7b-Antibody-3E10-ELISA-H00000540-M01-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (9H8) [NBP3-26489] - Staining of Hela cell with ATP7b Antibody (9H8) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26489_rabbit-atp7b-mab-9h8-28220241312249.jpg "Immunocytochemistry/Immunofluorescence: ATP7b Antibody (9H8) [NBP3-26489] - Staining of Hela cell with ATP7b Antibody (9H8) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: ATP7b Antibody (9H8) [NBP3-26489] - Overlay Peak curve showing Hela cells stained with ATP7b Antibody (9H8) (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26489_rabbit-atp7b-mab-9h8-282202414312774.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NBP2-58923] - Staining of human cell line CACO-2 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58923-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NBP2-58923] - Staining of human cell line CACO-2 shows localization to the Golgi apparatus.")