ATOX1 Antibody

Images

 
Western Blot: ATOX1 Antibody [NBP1-06611] - HeLa whole cell extract.
Immunocytochemistry/ Immunofluorescence: ATOX1 Antibody [NBP1-06611] - ATOX1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATOX1 Antibody Summary

Immunogen
Full length human ATOX1 protein [Swiss-Prot# O00244]
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATOX1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This ATOX1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In Western blot a band is seen at ~6 kDa representing ATOX1 protein. In Immunocytochemistry/Immunofluorescence cytoplasmic staining is observed in HeLa cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
ATOX1 Lysate (NBL1-07805)
HeLa Lysate (NB800-PC9)
Publications
Read Publications using
NBP1-06611 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for ATOX1 Antibody

  • ATX1 (antioxidant protein 1, yeast) homolog 1
  • ATX1 antioxidant protein 1 homolog (yeast)
  • ATX1
  • copper transport protein ATOX1
  • HAH1 MGC138453
  • hah1
  • Metal transport protein ATX1
  • MGC138455

Background

ATOX1 (anti-oxidant 1 or HAH1) is an ATX1 family member and is a ubiquitously expressed metallochaperone which binds/deliver cytosolic copper to copper ATPase proteins. Copper is an essential trace element which at high concentration is extremely toxic and a balance between copper import/export is highly critical for survival. To ensure that the total pool of free copper is less than 1 ion per cell, ATOX1 specifically deliver copper to cellular compartments and copper-dependent enzymes. ATOX1 interacts with ATP7A (Menkes protein) and ATP7B (Wilson protein) in a copper-dependent manner leading to facilitation of copper transfer from ATOX1 to ATP7A or ATP7B, thus permitting copper transport into the secretory pathway and copper export from the cell. The conserved metal binding site of ATOX1, CxGC, differs from the metal-binding sites of Cu-transporting ATPases and has a physiologically relevant redox potential that equilibrates with the GSH: GSSH pair. Cellular functions of ATOX1 are not limited to its copper-trafficking role and may include storage of labile copper, modulation of transcription, and antioxidant defense.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATOX1 Antibody (NBP1-06611)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ATOX1
Entrez
Uniprot