Recombinant Human CCL16/HCC-4 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CCL16/HCC-4 Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. Hedrick, J. et al. (1998) Blood 91:4242. The ED₅₀ for this effect is 0.5‑2 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 0.15‑0.75 µg/mL.
• Source: E. coli-derived human CCL16/HCC-4 protein
  Gln24-Gln120
• Accession #: O15467
• N-terminal Sequence: Gln24
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL16
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL16/HCC-4 Protein, CF

• C-C motif chemokine 16
• CCL16
• chemokine (C-C motif) ligand 16
• Chemokine CC-4
• Chemokine LEC
• CKb12
• HCC4
• HCC-4
• HCC-4Liver-expressed chemokine
• LCC-1Lymphocyte and monocyte chemoattractant
• LEC
• liver CC chemokine-1
• LMC
• LMCSmall-inducible cytokine A16
• monotactin-1
• Mtn-1
• NCC-4
• NCC4IL-10-inducible chemokine
• NCC-4ILINCK
• new CC chemokine 4
• SCYA16MGC117051
• SCYL4
• small inducible cytokine subfamily A (Cys-Cys), member 16

Background

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered.

Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.

1. Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273.
2. Hedrick, J. et al. (1998) Blood 91:4242.
3. Youn, B-S. et al. (1998) BBRC 247:217.

Publications for CCL16/HCC-4/LEC (802-HC/CF)(6)

Publications using 802-HC/CF

• V Picher-Mar, H Boutej, A Vézina, P Cordeau, H Kaneb, JP Julien, A Genge, N Dupré, J Kriz Distinct Plasma Immune Profile in ALS Implicates sTNFR-II in pAMPK/Leptin Homeostasis International Journal of Molecular Sciences, 2023-03-07;24(6):. 2023-03-07 [PMID: 36982140] (Bioassay, Human)
• Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533380] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Jang SW, Kim YS, Kim YR, Sung HJ, Ko J Regulation of human LZIP expression by NF-kappaB and its involvement in monocyte cell migration induced by Lkn-1. J. Biol. Chem., 2007-02-12;282(15):11092-100. 2007-02-12 [PMID: 17296613] (Bioassay, Human)
• Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006-04-17;350(2):484-92. 2006-04-17 [PMID: 16603217] (Binding Assay, Bioassay, Virus)
• Cappello P, Caorsi C, Bosticardo M CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity. J. Leukoc. Biol., 2003-10-02;75(1):135-42. 2003-10-02 [PMID: 14525962] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: CCL16
• Uniprot:
  • O15467()