SLC31A1/CTR1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SLC31A1/CTR1 Antibody [NB100-402] - CTR1 antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry: SLC31A1/CTR1 Antibody [NB100-402] - Panel 1: human CTR1 staining of breast cancer tissue. Panel 2: human CTR1-antigen competition in breast cancer tissue.
Immunohistochemistry: SLC31A1/CTR1 Antibody [NB100-402] - The photo on the left is antibody staining of hCTR1 overexpressing cell line. The photo on the right is antibody plus peptide competition staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

SLC31A1/CTR1 Antibody Summary

Immunogen
A synthetic peptide derived from a C-terminal sequence of human SLC31A1/CTR1 [UniProt# O15431]
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC31A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:250
Application Notes
This SLC31A1/CTR1 antibody is useful for Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin embedded sections..
Control Peptide
SLC31A1/CTR1 Peptide (NB100-402PEP)
Publications
Read Publications using
NB100-402 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SLC31A1/CTR1 Antibody

  • Copper transporter 1
  • COPT1high affinity copper uptake protein 1
  • CTR1MGC75487
  • hCTR1copper transport 1 homolog
  • solute carrier family 31 (copper transporters), member 1
  • Solute carrier family 31 member 1

Background

SLC31A1/CTR1 (solute carrier family 31 member 1/copper transporter 1) is a homotrimeric high-affinity, saturable copper importer implicated in dietary copper uptake. Homeostasis of copper is tightly controlled by inter-regulatory circuitry containing copper, Sp1, and CTR1, wherein Sp1 transcription factor acts as a copper sensor in modulating CTR1 expression which in turn controls cellular copper and Sp1 levels in a 3-way mutual regulatory loop. CTR1 mediated copper uptake is time, temperature, and pH dependent and specific for reduced form of Cu (I) and copper reduction has been proposed to be caused by Steap protein family or Dcytb protein (Cybrd1) both of which are reported to possess cupric reductase activity. The intracellular localization of CTR1 is variable in different cell lines: CTR1 is localized at the plasma membrane in HEK293, CaCo-2, and A2780 cells, whereas, in other cells such as HeLa, it is predominantly localized to vesicular compartments, and variability in trafficking rates between vesicular and plasma membrane compartments has been suggested as the cause of this differential localization. Post-translational O-linked glycosylation of CTR1 protects it against proteolytic cleavage of N terminal 17-kD fragment and the biological function of this cleaved variant or protease responsible is largely unknown. Selective knockout of Ctr1 in murine intestinal epithelial cells results in severe systemic copper deficiency, ataxia, and death prior to weaning.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for SLC31A1/CTR1 Antibody (NB100-402)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(2)
IHC
(2)
IHC-Fr
(2)
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-402 Applications Species
Doherty Ben, Lawlor Denise, Gillet Jean-Pierre et al. Collateral sensitivity to cisplatin in KB-8-5-11 drug-resistant cancer cells. Anticancer Res. 2014 Jan 01 [PMID: 24403508] (Human) Human
Yoshida H, Teramae M, Yamauchi M et al. Association of copper transporter expression with platinum resistance in epithelial ovarian cancer Anticancer Res 2013 Apr [PMID: 23564780]
Ip V, Liu JJ, McKeage MJ et al. Evaluation of effects of copper histidine on Ctr1-mediated accumulation of platinum and oxaliplatin-induced neurotoxicity in vitro and in vivo. Clin Exp Pharmacol Physiol 2013 Apr 5 [PMID: 23556474] (IHC, Rat) IHC Rat
Liu JJ, Kim Y, Yan F et al. Contributions of rat Ctr1 to the uptake and toxicity of copper and platinum anticancer drugs in dorsal root ganglion neurons Biochem Pharmacol 2012 Oct 31 [PMID: 23123662] (ICC/IF, Rat) ICC/IF Rat
Stordal B, Hamon M, McEneaney V et al. Resistance to Paclitaxel in a Cisplatin-Resistant Ovarian Cancer Cell Line Is Mediated by P-Glycoprotein plos One 2012 [PMID: 22792399] (ICC/IF, WB, Human) ICC/IF, WB Human
Pourvali K, Matak P, Latunde-Dada GOet al. Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2? FEBS Lett 2012 Jun 7 [PMID: 22684009] (WB, Human) WB Human
Chen DB, Feng L, Lin XP, Zhang W, Li FR, Liang XL, Li XH. Penicillamine increases free copper and enhances oxidative stress in the brain of toxic milk mice. PLoS One;7(5):e37709. 2012 [PMID: 22629446] (WB, IHC-Fr, IHC, Mouse) WB, IHC-Fr, IHC Mouse
More SS, Akil O, Ianculescu AG et al. Role of the copper transporter, CTR1, in platinum-induced ototoxicity. J Neurosci;30(28):9500-9. 2010 Jul 14. [PMID: 20631178] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Jandial DD et al. Enhanced delivery of cisplatin to intraperitoneal ovarian carcinomas mediated by the effects of bortezomib on the human copper transporter 1. Clin Cancer Res;15(2):553-60. 2009 Jan 15. [PMID: 19147760]
Peng, Fangyu et al. PET of human prostate cancer xenografts in mice with increased uptake of 64CuCl2. J Nucl Med;47(10):1649-52. 2006 Oct. [PMID: 17015901]
Show All 11 Publications.

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Product General Protocols

View specific protocols for SLC31A1/CTR1 Antibody (NB100-402): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC31A1/CTR1 Antibody (NB100-402). (Showing 1 - 2 of 2 FAQs).

  1. Could you provide the immunogen sequence of this antibody?
    • The immunogen sequence for this product is considered proprietary, but I can tell you it falls within amino acids 160-190 of CTR1.
  2. We have bought and used the SLC31A1/CTR1 antibody (Novus Biologicals; catnr. NB100-402) successfully for our Western blot and IHC analysis. We want to publish this, but the reviewers urged us to do a control experiment using a corresponding blocking peptide. Do you have the peptide available by which we can block the specific binding of this anti-SLC31A1 antibody?
    • Thank you for contacting us regarding your successful use of our product. In regards to your inquiry, I have done a search of our products, and unfortunately, we do not have the blocking peptide for that particular antibody (NB100-402). I apologize for any inconvenience this may cause you.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-402AF405
Alexa Fluor 488 Labeled NB100-402AF488
Alexa Fluor 647 Labeled NB100-402AF647
Alexa Fluor 700 Labeled NB100-402AF700
Biotin Labeled NB100-402B
DyLight 405 Labeled NB100-402V
DyLight 488 Labeled NB100-402G
DyLight 550 Labeled NB100-402R
DyLight 650 Labeled NB100-402C
FITC Labeled NB100-402F
HRP Labeled NB100-402H

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Bioinformatics Tool for SLC31A1/CTR1 Antibody (NB100-402)

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Blogs on SLC31A1/CTR1.

SLC31A1/CTR1 - a copper transporter with important implications for platinum-based chemotherapy
Copper is an essential micronutrient that serves as a cofactor in numerous biological processes, but can be toxic when present in excess. Because of this, cells must tightly maintain copper levels. This includes balance between import and export of...  Read full blog post.

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Bioinformatics

Gene Symbol SLC31A1