Immunocytochemistry/ Immunofluorescence: SLC31A1/CTR1 Antibody [NB100-402] - CTR1 antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry: SLC31A1/CTR1 Antibody [NB100-402] - Panel 1: human CTR1 staining of breast cancer tissue. Panel 2: human CTR1-antigen competition in breast cancer tissue.
Immunohistochemistry: SLC31A1/CTR1 Antibody [NB100-402] - The photo on the left is antibody staining of hCTR1 overexpressing cell line. The photo on the right is antibody plus peptide competition staining.
solute carrier family 31 (copper transporters), member 1
Solute carrier family 31 member 1
SLC31A1/CTR1 (solute carrier family 31 member 1/copper transporter 1) is a homotrimeric high-affinity, saturable copper importer implicated in dietary copper uptake. Homeostasis of copper is tightly controlled by inter-regulatory circuitry containing copper, Sp1, and CTR1, wherein Sp1 transcription factor acts as a copper sensor in modulating CTR1 expression which in turn controls cellular copper and Sp1 levels in a 3-way mutual regulatory loop. CTR1 mediated copper uptake is time, temperature, and pH dependent and specific for reduced form of Cu (I) and copper reduction has been proposed to be caused by Steap protein family or Dcytb protein (Cybrd1) both of which are reported to possess cupric reductase activity. The intracellular localization of CTR1 is variable in different cell lines: CTR1 is localized at the plasma membrane in HEK293, CaCo-2, and A2780 cells, whereas, in other cells such as HeLa, it is predominantly localized to vesicular compartments, and variability in trafficking rates between vesicular and plasma membrane compartments has been suggested as the cause of this differential localization. Post-translational O-linked glycosylation of CTR1 protects it against proteolytic cleavage of N terminal 17-kD fragment and the biological function of this cleaved variant or protease responsible is largely unknown. Selective knockout of Ctr1 in murine intestinal epithelial cells results in severe systemic copper deficiency, ataxia, and death prior to weaning.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for SLC31A1/CTR1 Antibody (NB100-402). (Showing 1 - 4 of 4 FAQs).
Could you provide the immunogen sequence of this antibody?
The immunogen sequence for this product is considered proprietary, but I can tell you it falls within amino acids 160-190 of CTR1.
We have bought and used the SLC31A1/CTR1 antibody (Novus Biologicals; catnr. NB100-402) successfully for our Western blot and IHC analysis. We want to publish this, but the reviewers urged us to do a control experiment using a corresponding blocking peptide. Do you have the peptide available by which we can block the specific binding of this anti-SLC31A1 antibody?
Thank you for contacting us regarding your successful use of our product. In regards to your inquiry, I have done a search of our products, and unfortunately, we do not have the blocking peptide for that particular antibody (NB100-402). I apologize for any inconvenience this may cause you.
Could you please tell me the targeted amino acids of the CTR1 antibody NB100-402?
Unfortunately, we cannot disclose that information. However, since human SLC31A1 is a small protein (http://www.uniprot.org/uniprot/O15431#sequences), I anticipate that so called ".. A synthetic peptide derived from a C-terminal sequence.. " should be located within a.a. 140 - 190.
What is the molecular weight of CTR1?
The theoretical molecular weight of CTR1 is 21kDa. The protein can run slightly higher in western blot due to PTMs.
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