DMBT1/GP340 Antibody (548012)

Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse DMBT1 Monoclonal Antibody (Catalog # MAB59151) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DMBT1 at approximately 210 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 548012
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

DMBT1/GP340 Antibody (548012) Summary

• Immunogen: E.coli-derived recombinant mouse DMBT1
  Trp215-Gly420
  Accession # Q60997
• Specificity: Detects mouse DMBT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD163 or recombinant human SRCRB4D is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: DMBT1
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DMBT1/GP340 Antibody (548012)

• CRP-ductin
• deleted in malignant brain tumors 1 protein
• deleted in malignant brain tumors 1
• DMBT1
• Ebnerin
• FLJ61058
• Glycoprotein 340
• GP340
• gp-340
• GP340muclin
• Hensin
• MGC164738
• Muclin
• SAG
• Salivary agglutinin
• Surfactant pulmonary-associated D-binding protein
• Vomeroglandin

Background

 

DMBT1 (deleted in malignant brain tumors 1), also called salivary agglutinin (SAG) in humans, hensin in rabbit, ebnerin in rat, and muclin, crp-ductin or vomeroglandin in mouse, is a transmembrane or secreted 2085 amino acid (aa) protein of the scavenger receptor cysteine-rich (SRCR) family. It contains several SRCR and CUB domains and a zona pellucida motif. The region used as the immunogen (aa 215-420) contains most of SRCR 2 and 3, and shares ~74% aa identity with corresponding regions of rat or human DMBT1. Several alternately spliced, glycosylated and sulfated forms exist. DMBT1 functions in mucosal immunity by binding bacterial, viral and endogenous proteins to aid clearance by macrophages. It is also thought to play a role in epithelial cell differentiation and tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DMBT1/GP340 Antibody (MAB59151)(2)

Publications using MAB59151

• TB Tsay, MC Yang, WH Chang, PH Chen, LW Chen Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model J Transl Med, 2018-08-13;16(1):225. 2018-08-13 [PMID: 30103798] (Western Blot, Mouse)
• Wu Y, Hsu C, Chen P, Fung C, Chen L Toll-like receptor stimulation induces nondefensin protein expression and reverses antibiotic-induced gut defense impairment. Infect Immun, 2014-03-04;82(5):1994-2005. 2014-03-04 [PMID: 24595141] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: DMBT1
• Uniprot:
  • Q60997()