COMMD1 Antibody (OTI1F2)

Western Blot: COMMD1 Antibody (1F2) [NBP2-03755] - Analysis of extracts (35ug) from 9 different cell lines by using anti-COMMD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry-Paraffin: COMMD1 Antibody (1F2) [NBP2-03755] - Staining of paraffin-embedded Human prostate tissue using anti-COMMD1 mouse monoclonal antibody.

Flow Cytometry: COMMD1 Antibody (1F2) [NBP2-03755] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-COMMD1 antibody, and then analyzed by flow cytometry.

Western Blot: COMMD1 Antibody (1F2) [NBP2-03755] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COMMD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COMMD1.

Immunohistochemistry-Paraffin: COMMD1 Antibody (1F2) [NBP2-03755] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COMMD1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: COMMD1 Antibody (1F2) [NBP2-03755] - Staining of paraffin-embedded Human colon tissue using anti-COMMD1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: COMMD1 Antibody (1F2) [NBP2-03755] - Staining of paraffin-embedded Human Kidney tissue using anti-COMMD1 mouse monoclonal antibody.

Flow Cytometry: COMMD1 Antibody (1F2) [NBP2-03755] - Analysis of Jurkat cells, using anti-COMMD1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, IHC
• Clone: OTI1F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

COMMD1 Antibody (OTI1F2) Summary

• Description: Novus Biologicals Mouse COMMD1 Antibody (OTI1F2) (NBP2-03755) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-COMMD1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human COMMD1(NP_689729) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: COMMD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COMMD1 Antibody (OTI1F2)

• C2orf5
• COMM domain-containing protein 1
• COMMD1
• copper metabolism (Murr1) domain containing 1
• copper metabolism gene MURR1
• MGC27155
• MURR1
• MURR1chromosome 2 open reading frame 5 (MURR1)
• Protein Murr1

Background

COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al., 2005 [PubMed 16267171]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COMMD1 Antibody (NBP2-03755)(1)

Publication using NBP2-03755

• Amika Singla, Alina Fedoseienko, Sai S. P. Giridharan, Brittany L. Overlee, Adam Lopez, Da Jia, Jie Song, Kayci Huff-Hardy, Lois Weisman, Ezra Burstein, Daniel D. Billadeau Endosomal PI(3)P regulation by the COMMD/CCDC22/CCDC93 (CCC) complex controls membrane protein recycling Nature Communications 2019-09-19 [PMID: 31537807]

Bioinformatics

• Gene Symbol: COMMD1
• Entrez:
  • 150684(Human)