ATP7b Antibody [HRP]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP

ATP7b Antibody [HRP] Summary

• Immunogen: A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b. [UniProt# P35670]
• Localization: Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140 kDa is located in mitochondria.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP7B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This ATP7b antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. By WB, this antibody recognizes a band at 165 kDa, representing ATP7b and also recognizes a faint non-specific band at ~195 kDa. In IHC-P, cytoplasmic and golgi staining was observed in mouse liver tissue (1:100 dilution) and human ovarian carcinoma cells (1:500 dilution). However, please note we also tested this antibody with IHC-P in mouse intestines and kidney and observed unexpected strong nuclear signal with lighter cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Human, rat and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ATP7b Antibody [HRP]

• ATPase, Cu(2+)- transporting, beta polypeptide
• ATPase, Cu++ transporting, beta polypeptide
• Copper pump 2
• copper-transporting ATPase 2
• EC 3.6.3
• EC 3.6.3.4
• PWD
• WC1
• WD
• Wilson disease-associated protein
• WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Background

ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP7B