ATP7b Antibody

Images

 
Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.
Immunocytochemistry/ Immunofluorescence: ATP7b Antibody [NB100-360] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry: ATP7b Antibody [NB100-360] - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

ATP7b Antibody Summary

Immunogen
A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b. [UniProt# P35670]
Localization
Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140 kDa is located in mitochondria.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP7B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation
Application Notes
This ATP7b antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. By WB, this antibody recognizes a band at 165 kDa, representing ATP7b and also recognizes a faint non-specific band at ~195 kDa. In IHC-P, cytoplasmic and golgi staining was observed in mouse liver tissue (1:100 dilution) and human ovarian carcinoma cells (1:500 dilution). However, please note we also tested this antibody with IHC-P in mouse intestines and kidney and observed unexpected strong nuclear signal with lighter cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
ATP7b Lysate (NBL1-07848)
Reviewed Applications
Read 1 Review rated 4
using
NB100-360 in the following applications:

Publications
Read Publications using
NB100-360 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Human, rat and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATP7b Antibody

  • ATPase, Cu(2+)- transporting, beta polypeptide
  • ATPase, Cu++ transporting, beta polypeptide
  • Copper pump 2
  • copper-transporting ATPase 2
  • EC 3.6.3
  • EC 3.6.3.4
  • PWD
  • WC1
  • WD
  • Wilson disease-associated protein
  • WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Background

ATP7b (copper-transporting P-type adenosine triphosphatase) is also called copper-transporting ATPase 2 or Wilson disease-associated protein and is involved in copper export out of the cells, such as the efflux of hepatic copper into the bile. ATP7b is a monomeric protein that interacts with COMMD1/MURR1, DCTN4 (in copper-dependent manner) and ATOX1. Predominantly localized in the trans-Golgi network (TGN), ATP7b is most abundantly expressed in liver, kidney and the brain tissues (isoform 2 is expressed in cytoplasm of brain cells but not in liver). Isoform 1 is proteolytically cleaved at N-terminus to produce the WND/140 kD form which is found in mitochondrions of hepatocytes as well as other tissue/cell types. Defects in ATP7B have been linked to Wilson disease (WD), an autosomal recessive disorder of copper metabolism, wherein, copper cannot be incorporated into hepatic ceruloplasmin and cannot be excreted from the liver into the bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP7b Antibody (NB100-360)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
IP
(1)
WB
(5)
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Filter By Species
Human
(3)
Mouse
(1)
Rat
(2)
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Showing Publications 1 - 8 of 8.
Publications using NB100-360 Applications Species
Chen SJ, Kuo CC, Pan HY et al. Mechanistic basis of a combination d-penicillamine and platinum drugs synergistically inhibits tumor growth in oxaliplatin-resistant human cervical cancer cells in vitro and in vivo. Biochem. Pharmacol. 2015 Mar 20 [PMID: 25801007] (WB, Human) WB Human
Gu S, Yang H, Qi Y et al. Novel ATPase Cu2+ Transporting Beta Polypeptide Mutations in Chinese Families with Wilson's Disease. PLoS One 2013 Jul 2 [PMID: 23843956] (WB, Human) WB Human
Katagiri H, Nakayama K, Rahman MT et al. Is ATP7B a Predictive Marker in Patients With Ovarian Carcinoma Treated With Platinum-Taxane Combination Chemotherapy? Int J Gynecol Cancer 2013 Jan [PMID: 23221602]
Ip V, Liu JJ, Mercer JF et al. Differential expression of ATP7A, ATP7B and CTR1 in adult rat dorsal root ganglion tissue. Mol Pain;6(1):53. 2010 Sep 13. [PMID: 20836889] (ICC/IF, IHC, Rat) ICC/IF, IHC Rat
Mangala LS, Zuzel V, Schmandt R et al. Therapeutic Targeting of ATP7B in Ovarian Carcinoma. Clin Cancer Res 2009 Jun 1 [PMID: 19470734] (WB, Human) WB Human
Goss JA, Barshes NR, Karpen SJ et al. Liver ischemia and ischemia-reperfusion induces and trafficks the multi-specific metal transporter Atp7b to bile duct canaliculi: possible preferential transport of iron into bile. Biol Trace Elem Res. 2008 Apr [PMID: 17987273] (WB, IHC-P, Rat) WB, IHC-P Rat
Safaei R et al. Transport of cisplatin by the copper efflux transporter ATP7B. Mol Pharmacol;73(2):461-8. 2008 Feb. [PMID: 17978167] (WB, IP) WB, IP
Niciu MJ, Ma XM, El Meskini R et al. Altered ATP7A expression and other compensatory responses in a murine model of Menkes disease. Neurobiol Dis 2007 Sep [PMID: 17588765] (Mouse) Mouse

Review for ATP7b Antibody (NB100-360) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-360:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ATP7b NB100-360
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reviewed by:
Anonymous
WB Human 09/07/2015
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates for ovarian cancer cell lines A2780 and its cisplatin resistant variant A2780-CP20.
SpeciesHuman
LotB-2

Blocking

Blocking Details4% BSA in TBST for 1h at room temperature

Primary Anitbody

Dilution Ratio1:1000 in TBST + 1%BSA, overnight, 4*C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2301
Secondary Concentration1:10,000

Details

Detection NotesECL

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATP7b Antibody (NB100-360). (Showing 1 - 3 of 3 FAQs).

  1. Which isoform of the protein does NB100-360 recognize?
    • The immunogen for this product is to the N-terminal, so it will recognize all 4 known isoforms of ATP7b.
  2. Would you be able to provide any additional information about the sequence or location of the immunogenic peptide?
    • I cannot give the exact immunogen sequence as it is considered to be propriety information; however, I am able to provide a range. The immunogen of NB100-360 lies within aa 300-350 of the human ATP7b protein.
  3. Can you disclose any further detail related to the immunogen sequence used to generate NB100-360?
    • A more specific range for the immunogen of our NB100-360 is aa300-350 of the human ATP7b protein 

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-360AF405
Alexa Fluor 488 Labeled NB100-360AF488
Alexa Fluor 647 Labeled NB100-360AF647
Alexa Fluor 700 Labeled NB100-360AF700
Biotin Labeled NB100-360B
DyLight 405 Labeled NB100-360V
DyLight 488 Labeled NB100-360G
DyLight 550 Labeled NB100-360R
DyLight 650 Labeled NB100-360C
FITC Labeled NB100-360F
HRP Labeled NB100-360H

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Recent Reviews

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Anonymous
09/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATP7B