ATP7b Antibody - BSA Free

Images

 
Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.
Immunocytochemistry/ Immunofluorescence: ATP7b Antibody [NB100-360] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry: ATP7b Antibody [NB100-360] - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

ATP7b Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b. [UniProt# P35670]
Localization
Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140 kDa is located in mitochondria.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP7B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting reported in scientific literature (PMID 29250175)
  • Immunocytochemistry/Immunofluorescence 2 - 10 ug/mL
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
In WB this antibody recognizes a band at 165 kDa, representing ATP7b and also recognizes a faint non-specific band at ~195 kDa. In IHC-P, cytoplasmic and golgi staining was observed in mouse liver tissue (1:100 dilution) and human ovarian carcinoma cells (1:500 dilution). However, please note we also tested this antibody with IHC-P in mouse intestines and kidney and observed unexpected strong nuclear signal with lighter cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ATP7b Overexpression Lysate
Control Peptide
ATP7b Protein (NB100-360PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB100-360 in the following applications:

Publications
Read Publications using
NB100-360 in the following applications:

  • 1 publication
  • IB
    1 publication
  • 2 publications
  • 2 publications
  • 2 publications
  • IP
    1 publication
  • WB
    12 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATP7b Antibody - BSA Free

  • ATPase, Cu(2+)- transporting, beta polypeptide
  • ATPase, Cu++ transporting, beta polypeptide
  • Copper pump 2
  • copper-transporting ATPase 2
  • EC 3.6.3
  • EC 3.6.3.4
  • PWD
  • WC1
  • WD
  • Wilson disease-associated protein
  • WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Background

ATP7b (copper-transporting P-type adenosine triphosphatase) is also called copper-transporting ATPase 2 or Wilson disease-associated protein and is involved in copper export out of the cells, such as the efflux of hepatic copper into the bile. ATP7b is a monomeric protein that interacts with COMMD1/MURR1, DCTN4 (in copper-dependent manner) and ATOX1. Predominantly localized in the trans-Golgi network (TGN), ATP7b is most abundantly expressed in liver, kidney and the brain tissues (isoform 2 is expressed in cytoplasm of brain cells but not in liver). Isoform 1 is proteolytically cleaved at N-terminus to produce the WND/140 kD form which is found in mitochondrions of hepatocytes as well as other tissue/cell types. Defects in ATP7B have been linked to Wilson disease (WD), an autosomal recessive disorder of copper metabolism, wherein, copper cannot be incorporated into hepatic ceruloplasmin and cannot be excreted from the liver into the bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP7b Antibody (NB100-360)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: FLOW, IB, ICC/IF, IF/IHC, IHC-P, IP, WB.


Filter By Application
FLOW
(1)
IB
(1)
ICC/IF
(2)
IF/IHC
(2)
IHC-P
(2)
IP
(1)
WB
(12)
All Applications
Filter By Species
Human
(10)
Mouse
(2)
Rat
(3)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-360 Applications Species
Shin VY, Liu MX, Siu JM et al. Inhibition of EP2 receptor suppresses tumor growth and chemoresistance of gastric cancer American journal of cancer research 2022-10-15 [PMID: 36381319] (WB, Human) WB Human
Xia F, Fu Y, Xie H et al. Suppression of ATG4B by copper inhibits autophagy and involves in Mallory body formation Redox biology 2022-03-24 [PMID: 35349929] (WB, Human) WB Human
Yang D, Wang T, Liu J et al. Reverse regulation of hepatic ceruloplasmin production in rat model of myocardial ischemia J Trace Elem Med Biol 2020-11-16 [PMID: 33249375] (WB, Rat)

Details:
Western blot analysis performed on livers of Sprague-Dawley rats that underwent myocardial infarction.
WB Rat
Ryumon S, Okui T, Kunisada Y et al. Ammonium tetrathiomolybdate enhances the antitumor effect of cisplatin via the suppression of ATPase copper transporting beta in head and neck squamous cell carcinoma Oncol. Rep. 2019-10-10 [PMID: 31638244] (WB, IF/IHC, Human) WB, IF/IHC Human
Han Y, Wang T, Liu H et al. The release and detection of copper ions from ultrasmall theranostic Cu2-xSe nanoparticles Nanoscale 2019-06-20 [PMID: 31184674]
Su TA, Shihadih DS, Cao W et al. A Modular Ionophore Platform for Liver-Directed Copper Supplementation in Cells and Animals. J. Am. Chem. Soc. 2018-10-15 [PMID: 30351140] (WB, Human) WB Human
Agarwal T, Rustagi A, Das J, Maiti TK. PAMAM dendrimer grafted cellulose paper scaffolds as a novel in vitro 3D liver model for drug screening applications. Colloids Surf B Biointerfaces 2018-08-29 [PMID: 30179804] (Human) Human
Li K, Li C, Xiao Y et al. The loss of copper is associated with the increase in copper metabolism MURR domain 1 in ischemic hearts of mice Exp. Biol. Med. (Maywood) 2018-05-01 [PMID: 29763364] (WB, Mouse) WB Mouse
Moinuddin F M, Hirano Hirofumi, Shinsato Yoshinari et al. ATP7B expression in human glioblastoma is related to temozolomide resistance. Oncology Letters 2017-10-23 [PMID: 29250175] (IHC-P, IB, Human) IHC-P, IB Human
Chen SJ, Kuo CC, Pan HY et al. Mechanistic basis of a combination d-penicillamine and platinum drugs synergistically inhibits tumor growth in oxaliplatin-resistant human cervical cancer cells in vitro and in vivo. Biochem. Pharmacol. 2015-03-20 [PMID: 25801007] (WB, Human) WB Human
Show All 19 Publications.

Review for ATP7b Antibody (NB100-360) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-360:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot ATP7b NB100-360
Enlarge
4
reviewed by:
Prabir Chakraborty
WB Human 09/07/2015
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates for ovarian cancer cell lines A2780 and its cisplatin resistant variant A2780-CP20.
SpeciesHuman
LotB-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATP7b Antibody (NB100-360). (Showing 1 - 3 of 3 FAQs).

  1. Which isoform of the protein does NB100-360 recognize?
    • The immunogen for this product is to the N-terminal, so it will recognize all 4 known isoforms of ATP7b.
  2. Would you be able to provide any additional information about the sequence or location of the immunogenic peptide?
    • I cannot give the exact immunogen sequence as it is considered to be propriety information; however, I am able to provide a range. The immunogen of NB100-360 lies within aa 300-350 of the human ATP7b protein.
  3. Can you disclose any further detail related to the immunogen sequence used to generate NB100-360?
    • A more specific range for the immunogen of our NB100-360 is aa300-350 of the human ATP7b protein 

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Prabir Chakraborty
09/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATP7B