Angiopoietin-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody [NBP2-15385] - Human colon carcinoma, using Angiopoietin 2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Angiopoietin-2 Antibody [NBP2-15385] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Angiopoietin-2 Antibody [NBP2-15385] - HepG2 conditioned medium (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

• Reactivity: Hu, Mu, Po, Ca
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Angiopoietin-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Angiopoietin-2 Antibody - BSA Free (NBP2-15385) is a polyclonal antibody validated for use in IHC and WB. Anti-Angiopoietin-2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Angiopoietin-2. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Porcine (92%), Canine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANGPT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (86%), Bovine (89%), Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Angiopoietin-2 Antibody - BSA Free

• AGPT2
• ANG2
• ANG-2
• angiopoietin 2
• Angiopoietin-2
• angiopoietin-2a
• angiopoietin-2B
• angiopoitin 2
• ANGPT2
• Tie2-ligand

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-2 Antibody (NBP2-15385)(2)

Publications using NBP2-15385

• Drosos I, Pavlaki M, Ortega Carrillo MDP et al. Increased Lymphangiogenesis and Lymphangiogenic Growth Factor Expression in Perivascular Adipose Tissue of Patients with Coronary Artery Disease J Clin Med 2019-07-09 [PMID: 31324038] (IF/IHC, Human)
• Zhou Hc, Fang Jh, Shang Lr et al. MicroRNA-125b and microRNA-100 suppress metastasis of hepatocellular carcinoma by disrupting the formation of vessels that encapsulated tumour clusters. J. Pathol. 2016-08-31 [PMID: 27577856] (IHC-P)

Bioinformatics

• Gene Symbol: ANGPT2