Human VEGF-C Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human VEGF-C Quantikine ELISA Kit Summary

• Background: The Quantikine Human VEGF-C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF-C in cell culture supernates, serum, and plasma. It contains recombinant human VEGF-C (Cys156Ser substituted) and has been shown to accurately quantitate the recombinant factor. Results obtained using recombinant human VEGF-C (wild type) and natural human VEGF-C showed linear curves that we...re parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VEGF-C.
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• Specificity: Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: VEGFC

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human VEGF-C Quantikine ELISA Kit

• Flt4 ligand
• Flt4-L
• vascular endothelial growth factor C
• Vascular endothelial growth factor-related protein
• VEGFC
• VEGF-C
• VRPFLT4 ligand DHM

Background

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VEGF-C (DVEC00)(55)

Publications using DVEC00

• Meshko, B;Volatier, TLA;Mann, J;Kluth, MA;Ganss, C;Frank, MH;Frank, NY;Ksander, BR;Cursiefen, C;Notara, M; Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population International journal of molecular sciences 2024-09-07 [PMID: 39273646] (Human)
• Francavilla, F;Sarcina, F;Schepetkin, IA;Kirpotina, LN;Contino, M;Schirizzi, A;De Leonardis, G;Khlebnikov, AI;D'Alessandro, R;Quinn, MT;Lacivita, E;Leopoldo, M; Development of potent isoflavone-based formyl peptide receptor 1 (FPR1) antagonists and their effects in gastric cancer cell models European journal of medicinal chemistry 2023-10-04 [PMID: 37839346] (Human)
• Chachaj, A;Stanimirova, I;Chabowski, M;Gomu?kiewicz, A;Hodurek, P;Glatzel-Pluci?ska, N;Olbromski, M;Piotrowska, A;Kuzan, A;Grzegrzó?ka, J;Ratajczak-Wielgomas, K;Nowak, A;Szahidewicz-Krupska, E;Wi?niewski, J;Bromke, MA;Podhorska-Oko?ów, M;Gamian, A;Janczak, D;Dzi?giel, P;Szuba, A; Sodium accumulation in the skin is associated with higher density of skin lymphatic vessels in patients with arterial hypertension Advances in medical sciences 2023-08-26 [PMID: 37639949] (Human)
• Szymczak, A;Kusztal, M;Go??biowski, T;Letachowicz, K;Go?dzik, A;Ko?cielska-Kasprzak, K;Tukiendorf, A;Krajewska, M; High Plasma Angiopoietin-2 Levels Predict the Need to Initiate Dialysis within Two Years in Patients with Chronic Kidney Disease International journal of molecular sciences 2023-06-12 [PMID: 37373181] (Human)
• G Iwanek, B Ponikowska, A Zdanowicz, M Fudim, M Hurkacz, R Zymli?ski, P Ponikowski, J Biegus Relationship of Vascular Endothelial Growth Factor C, a Lymphangiogenesis Modulator, with Edema Formation, Congestion and Outcomes in Acute Heart Failure Journal of cardiac failure, 2023-04-29;0(0):. 2023-04-29 [PMID: 37121266] (Human)
• J Suzuki, Y Shimizu, T Hayashi, Y Che, Z Pu, K Tsuzuki, S Narita, R Shibata, I Ishii, JW Calvert, T Murohara Hydrogen Sulfide Attenuates Lymphedema Via the Induction of Lymphangiogenesis Through a PI3K/Akt-Dependent Mechanism Journal of the American Heart Association, 2022-10-26;11(21):e026889. 2022-10-26 [PMID: 36285798] (Human)
• J Zhong, HC Yang, EL Shelton, T Matsusaka, AJ Clark, V Yermalitsk, Z Mashhadi, LS May-Zhang, MF Linton, AB Fogo, A Kirabo, SS Davies, V Kon Dicarbonyl-modified lipoproteins contribute to proteinuric kidney injury JCI Insight, 2022-11-08;7(21):. 2022-11-08 [PMID: 36125905] (Human)
• S Biswas Shi, Q Lu, D Sun, H Hou, JN Bulmer, BA Innes, DK Hapangama, GE Lash Platelet-derived growth factor BB is reduced in endometrial endothelial cells of women with abnormal uterine bleeding-endometrial disorder Oncogene, 2022-02-13;0(0):. 2022-02-13 [PMID: 35739007] (Human)
• IS Miller, S Khan, LP Shiels, S Das, AC O' Farrell, K Connor, A Lafferty, B Moran, C Isella, P Loadman, E Conroy, S Cohrs, R Schibli, RS Kerbel, WM Gallagher, E Marangoni, K Bennett, DP O' Connor, RM Dwyer, AT Byrne Implementing subtype-specific pre-clinical models of breast cancer to study pre-treatment aspirin effects Cancer Medicine, 2022-04-17;0(0):. 2022-04-17 [PMID: 35434898] (Human)
• Y Li, C Liu, X Zhang, X Huang, S Liang, F Xing, H Tian CCT5 induces epithelial-mesenchymal transition to promote gastric cancer lymph node metastasis by activating the Wnt/beta-catenin signalling pathway British Journal of Cancer, 2022-02-22;0(0):. 2022-02-22 [PMID: 35194191] (Human)
• I Wada, S Nakao, M Yamaguchi, Y Kaizu, M Arima, S Sawa, KH Sonoda Retinal VEGF-A Overexpression Is Not Sufficient to Induce Lymphangiogenesis Regardless of VEGF-C Upregulation and Lyve1+ Macrophage Infiltration Investigative Ophthalmology & Visual Science, 2021-10-04;62(13):17. 2021-10-04 [PMID: 34673901] (Human)
• T Mashima, T Wakatsuki, N Kawata, MK Jang, A Nagamori, H Yoshida, K Nakamura, T Migita, H Seimiya, K Yamaguchi Neutralization of the induced VEGF-A potentiates the therapeutic effect of an anti-VEGFR2 antibody on gastric cancer in vivo Scientific Reports, 2021-07-23;11(1):15125. 2021-07-23 [PMID: 34302038] (Mouse)
• M Sevinc, NB Hasbal, T Basturk, PN Ozcafer, BB Kocas, K Kilickesme, A Ozel, T Sakaci, E Ahbap, A Unsal, Y Koc Comparison of lung ultrasound and other volumetric methods in peritoneal dialysis patients Medicine, 2021-01-22;100(3):e23856. 2021-01-22 [PMID: 33545952] (Human)
• LouJin Song, Xian Chen, Terri A Swanson, Brianna LaViolette, Jincheng Pang, Teresa Cunio et al. Lymphangiogenic therapy prevents cardiac dysfunction by ameliorating inflammation and hypertension eLife 2020-11-17 [PMID: 33200983] (Mouse)
• WN Li, KY Hsiao, CA Wang, N Chang, PL Hsu, CH Sun, SR Wu, MH Wu, SJ Tsai Extracellular vesicle-associated VEGF-C promotes lymphangiogenesis and immune cells infiltration in endometriosis Proc Natl Acad Sci U S A, 2020-10-01;0(0):. 2020-10-01 [PMID: 33004630] (Human)
• C Reglero, V Lafarga, V Rivas, Á Albitre, P Ramos, SR Berciano, O Tapia, ML Martínez-C, F Mayor, P Penela GRK2-Dependent HuR Phosphorylation Regulates HIF1&alpha Activation under Hypoxia or Adrenergic Stress Cancers (Basel), 2020-05-13;12(5):. 2020-05-13 [PMID: 32413989] (Human)
• H Wada, M Suzuki, M Matsuda, Y Ajiro, T Shinozaki, S Sakagami, K Yonezawa, M Shimizu, J Funada, T Takenaka, Y Morita, T Nakamura, K Fujimoto, H Matsubara, T Kato, T Unoki, D Takagi, K Wada, M Wada, M Iguchi, N Masunaga, M Ishii, H Yamakage, T Kusakabe, A Yasoda, A Shimatsu, K Kotani, N Satoh-Asah, M Abe, M Akao, K Hasegawa Distinct Characteristics of VEGF-D and VEGF-C to Predict Mortality in Patients With Suspected or Known Coronary Artery Disease J Am Heart Assoc, 2020-04-22;9(9):e015761. 2020-04-22 [PMID: 32319336] (Human)
• AZ Dudek, LC Liu, S Gupta, TF Logan, EA Singer, M Joshi, YN Zakharia, JM Lang, JK Schwarz, A Al-Janadi, AS Alva Phase Ib/II Clinical Trial of Pembrolizumab With Bevacizumab for Metastatic Renal Cell Carcinoma: BTCRC-GU14-003 J. Clin. Oncol., 2020-02-25;38(11):1138-1145. 2020-02-25 [PMID: 32097091] (Human)
• SK Jha, K Rauniyar, E Chronowska, K Mattonet, EW Maina, H Koistinen, UH Stenman, K Alitalo, M Jeltsch KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D Elife, 2019-05-17;8(0):. 2019-05-17 [PMID: 31099754] (Human)
• R Wang, T Yamada, S Arai, K Fukuda, H Taniguchi, A Tanimoto, A Nishiyama, S Takeuchi, K Yamashita, K Ohtsubo, J Matsui, N Onoda, E Hirata, S Taira, S Yano Distribution and Activity of Lenvatinib in Brain Tumor Models of Human Anaplastic Thyroid Cancer Cells in Severe Combined Immune Deficient Mice Mol. Cancer Ther., 2019-03-29;18(5):947-956. 2019-03-29 [PMID: 30926637] (Human)
• S Schwager, S Renner, T Hemmerle, S Karaman, ST Proulx, R Fetz, AM Golding-Oc, P Probst, C Halin, D Neri, M Detmar Antibody-mediated delivery of VEGF-C potently reduces chronic skin inflammation JCI Insight, 2018-12-06;3(23):. 2018-12-06 [PMID: 30518687] (Mouse)
• AR Harris, MJ Perez, JM Munson Docetaxel facilitates lymphatic-tumor crosstalk to promote lymphangiogenesis and cancer progression BMC Cancer, 2018-07-06;18(1):718. 2018-07-06 [PMID: 29976154] (Mouse)
• CZ Li, XJ Jiang, B Lin, HJ Hong, SY Zhu, L Jiang, XQ Wang, NH Tang, FF She, YL Chen RIP1 regulates TNF-?-mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF-?B-VEGF-C pathway Onco Targets Ther, 2018-05-16;11(0):2875-2890. 2018-05-16 [PMID: 29844685] (Human)
• X Jiang, C Li, B Lin, H Hong, L Jiang, S Zhu, X Wang, N Tang, X Li, F She, Y Chen cIAP2 promotes gallbladder cancer invasion and lymphangiogenesis by activating the NF-?B pathway Cancer Sci., 2017-05-31;108(6):1144-1156. 2017-05-31 [PMID: 28295868] (Human)
• Débora C Bastos Effects of fatty acid synthase inhibitors on lymphatic vessels: an in vitro and in vivo study in a melanoma model Lab. Invest, 2016-12-05;0(0):. 2016-12-05 [PMID: 27918556] (Human)
• David M Briscoe Vascular endothelial growth factor A is associated with the subsequent development of moderate or severe cardiac allograft vasculopathy in pediatric heart transplant recipients J. Heart Lung Transplant., 2016-10-03;0(0):. 2016-10-03 [PMID: 27865734] (Human)
• Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling Sci Rep, 2016-07-04;6(0):29029. 2016-07-04 [PMID: 27374240] (Human)
• Tokumoto M, Tanaka H, Tauchi Y, Kasashima H, Kurata K, Yashiro M, Sakurai K, Toyokawa T, Kubo N, Amano R, Kimura K, Muguruma K, Maeda K, Ohira M, Hirakawa K Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer. Br J Cancer, 2015-09-10;113(7):1046-54. 2015-09-10 [PMID: 26355233] (Human)
• Tutunea-Fatan , Elena, Majumder , Mousumi, Xin , Xiping, Lala , Peeyush The role of CCL21/CCR7 chemokine axis in breast cancer-induced lymphangiogenesis. Mol Cancer, 2015-02-10;14(1):35. 2015-02-10 [PMID: 25744065] (Human)
• Kimura H, Mikami D, Kamiyama K, Sugimoto H, Kasuno K, Takahashi N, Yoshida H, Iwano M Telmisartan, a possible PPAR-delta agonist, reduces TNF-alpha-stimulated VEGF-C production by inhibiting the p38MAPK/HSP27 pathway in human proximal renal tubular cells. Biochem Biophys Res Commun, 2014-10-22;454(2):320-7. 2014-10-22 [PMID: 25450396] (Human)
• Sallinen H, Heikura T, Koponen J, Kosma V, Heinonen S, Yla-Herttuala S, Anttila M Serum angiopoietin-2 and soluble VEGFR-2 levels predict malignancy of ovarian neoplasm and poor prognosis in epithelial ovarian cancer. BMC Cancer, 2014-09-23;14(0):696. 2014-09-23 [PMID: 25245329] (Human)
• Weinkopff T, Mackenzie C, Eversole R, Lammie P Filarial excretory-secretory products induce human monocytes to produce lymphangiogenic mediators. PLoS Negl Trop Dis, 2014-07-10;8(7):e2893. 2014-07-10 [PMID: 25010672] (Human)
• Yang M, Kim H, Lee K, Yang S, Lee J, Lee K, Rhee J KML001 inhibits cell proliferation and invasion in pancreatic cancer cells through suppression of NF-kappaB and VEGF-C. Anticancer Res, 2014-07-01;34(7):3469-74. 2014-07-01 [PMID: 24982355] (Human)
• Decio A, Taraboletti G, Patton V, Alzani R, Perego P, Fruscio R, Jurgensmeier J, Giavazzi R, Belotti D Vascular endothelial growth factor c promotes ovarian carcinoma progression through paracrine and autocrine mechanisms. Am J Pathol, 2014-02-06;184(4):1050-61. 2014-02-06 [PMID: 24508126] (Human)
• Armstrong A, Haggman M, Stadler W, Gingrich J, Assikis V, Polikoff J, Damber J, Belkoff L, Nordle O, Forsberg G, Carducci M, Pili R Long-term survival and biomarker correlates of tasquinimod efficacy in a multicenter randomized study of men with minimally symptomatic metastatic castration-resistant prostate cancer. Clin Cancer Res, 2013-11-19;19(24):6891-901. 2013-11-19 [PMID: 24255071] (Human)
• Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy. Cytokine, 2013-02-14;61(3):755-8. 2013-02-14 [PMID: 23415672] (Human)
• Yazdani S, Poosti F, Kramer A, Mirkovic K, Kwakernaak A, Hovingh M, Slagman M, Sjollema K, de Borst M, Navis G, van Goor H, van den Born J Proteinuria triggers renal lymphangiogenesis prior to the development of interstitial fibrosis. PLoS ONE, 2012-11-26;7(11):e50209. 2012-11-26 [PMID: 23189189] (Human)
• Lin CE, Chen SU, Lin CC Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells. PLoS ONE, 2012-07-20;7(7):e41096. 2012-07-20 [PMID: 22911748] (Human)
• Majumder M, Tutunea-Fatan E, Xin X, Rodriguez-Torres M, Torres-Garcia J, Wiebe R, Timoshenko AV, Bhattacharjee RN, Chambers AF, Lala PK Co-expression of alpha9beta1 integrin and VEGF-D confers lymphatic metastatic ability to a human breast cancer cell line MDA-MB-468LN. PLoS ONE, 2012-04-24;7(4):e35094. 2012-04-24 [PMID: 22545097] (Human)
• Harmon CS, DePrimo SE, Raymond E, Cheng AL, Boucher E, Douillard JY, Lim HY, Kim JS, Lechuga MJ, Lanzalone S, Lin X, Faivre S Mechanism-related circulating proteins as biomarkers for clinical outcome in patients with unresectable hepatocellular carcinoma receiving sunitinib. J Transl Med, 2011-07-25;9(0):120. 2011-07-25 [PMID: 21787417] (Human)
• Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S Prognostic significance of angiogenic factors in uterine cervical cancer. Anticancer Res., 2011-08-01;31(8):2589-95. 2011-08-01 [PMID: 21778309] (Human)
• Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell, 2011-06-10;145(6):926-40. 2011-06-10 [PMID: 21663795] (Human)
• Konings IR, de Jonge MJ, Burger H Phase I and pharmacological study of the broad-spectrum tyrosine kinase inhibitor JNJ-26483327 in patients with advanced solid tumours. Br. J. Cancer, 2010-09-07;103(7):987-92. 2010-09-07 [PMID: 20823884] (Human)
• Ha CT, Wu JA, Irmak S, Lisboa FA, Dizon AM, Warren JW, Ergun S, Dveksler GS Human pregnancy specific beta-1-glycoprotein 1 (PSG1) has a potential role in placental vascular morphogenesis. Biol. Reprod., 2010-03-24;83(1):27-35. 2010-03-24 [PMID: 20335639] (Human)
• Onimaru M, Yonemitsu Y, Fujii T, Tanii M, Nakano T, Nakagawa K, Kohno R, Hasegawa M, Nishikawa S, Sueishi K VEGF-C regulates lymphangiogenesis and capillary stability by regulation of PDGF-B. Am. J. Physiol. Heart Circ. Physiol., 2009-09-04;297(5):H1685-96. 2009-09-04 [PMID: 19734356] (Human)
• Zhu AX, Sahani DV, Duda DG, di Tomaso E, Ancukiewicz M, Catalano OA, Sindhwani V, Blaszkowsky LS, Yoon SS, Lahdenranta J, Bhargava P, Meyerhardt J, Clark JW, Kwak EL, Hezel AF, Miksad R, Abrams TA, Enzinger PC, Fuchs CS, Ryan DP, Jain RK Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study. J. Clin. Oncol., 2009-05-26;27(18):3027-35. 2009-05-26 [PMID: 19470923] (Human)
• Machnik A, Neuhofer W, Jantsch J, Dahlmann A, Tammela T, Machura K, Park JK, Beck FX, Muller DN, Derer W, Goss J, Ziomber A, Dietsch P, Wagner H, Van Rooijen N, Kurtz A, Hilgers KF, Alitalo K, Eckardt KU, Luft FC, Kerjaschki D, Titze J Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C-dependent buffering mechanism. Nat. Med., 2009-05-03;15(5):545-52. 2009-05-03 [PMID: 19412173] (Human)
• Rini BI, Michaelson MD, Rosenberg JE, Bukowski RM, Sosman JA, Stadler WM, Hutson TE, Margolin K, Harmon CS, DePrimo SE, Kim ST, Chen I, George DJ Antitumor activity and biomarker analysis of sunitinib in patients with bevacizumab-refractory metastatic renal cell carcinoma. J. Clin. Oncol., 2008-08-01;26(22):3743-8. 2008-08-01 [PMID: 18669461] (Human)
• Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp. Hematol., 2008-05-27;36(8):1035-46. 2008-05-27 [PMID: 18504067] (Human)
• Huang V, Lonsdorf AS, Fang L, Kakinuma T, Lee VC, Cha E, Zhang H, Nagao K, Zaleska M, Olszewski WL, Hwang ST Cutting edge: rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells. J. Immunol., 2008-05-15;180(10):6462-6. 2008-05-15 [PMID: 18453562] (Human)
• Simiantonaki N, Jayasinghe C, Michel-Schmidt R, Peters K, Hermanns MI, Kirkpatrick CJ Hypoxia-induced epithelial VEGF-C/VEGFR-3 upregulation in carcinoma cell lines. Int. J. Oncol., 2008-03-01;32(3):585-92. 2008-03-01 [PMID: 18292935] (Human)
• Lee CY, Tien HF, Hu CY, Chou WC, Lin LI Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia. Br. J. Cancer, 2007-09-11;97(7):877-82. 2007-09-11 [PMID: 17848952] (Human)
• Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts. Stem Cells, 2007-04-19;25(8):1862-71. 2007-04-19 [PMID: 17446561] (Human)
• Laakkonen P, Waltari M, Holopainen T, Takahashi T, Pytowski B, Steiner P, Hicklin D, Persaud K, Tonra JR, Witte L, Alitalo K Vascular endothelial growth factor receptor 3 is involved in tumor angiogenesis and growth. Cancer Res., 2007-01-15;67(2):593-9. 2007-01-15 [PMID: 17234768] (Human)
• Lin J, Lalani AS, Harding TC Inhibition of lymphogenous metastasis using adeno-associated virus-mediated gene transfer of a soluble VEGFR-3 decoy receptor. Cancer Res., 2005-08-01;65(15):6901-9. 2005-08-01 [PMID: 16061674] (Human)

FAQs for VEGF-C (DVEC00). (Showing 1 - 2 of 2 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VEGFC