Tie-2 Antibody [Unconjugated]

Images

 
Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by ...read more
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Tie‑2 at approximately 161 kDa (as indicated) using 10 µg/mL of Goat ...read more
Tie‑2 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) at 10 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Tie-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tie‑2
Ala23-Lys745
Accession # Q02763
Specificity
Detects human Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Tie-2 is observed and less than 1% cross-reactivity with recombinant human Tie-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TEK
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF313 in the following applications:

Publications
Read Publications using
AF313 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-2 Antibody [Unconjugated]

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
  3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tie-2 Antibody (AF313)(27)

We have publications tested in 4 confirmed species: Human, Mouse, Primate - Papio anubis (Olive Baboon), Rabbit.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(4)
IHC
(1)
IHC-P
(1)
Immunoprecipitation
(5)
Neutralization
(4)
Western Blot
(11)
All Applications
Filter By Species
Human
(23)
Mouse
(1)
Primate - Papio anubis (Olive Baboon)
(1)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using AF313 Applications Species
LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions PloS Pathogens, 2022;18(1):e1010280. 2022 [PMID: 35100311] (Western Blot, Human) Western Blot Human
M Adjuto-Sac, P Soubeyran, J Garcia, S Audebert, L Camoin, M Rubis, J Roques, B Binétruy, JL Iovanna, R Tournaire TNF-&amp;alpha induces endothelial-mesenchymal transition promoting stromal development of pancreatic adenocarcinoma Cell Death & Disease, 2021;12(7):649. 2021 [PMID: 34172716] (Immunoprecipitation, Human) Immunoprecipitation Human
F Heil, G Babitzki, A Julien-Laf, CH Ooi, M Hidalgo, C Massard, M Martinez-G, C Le Tournea, M Kockx, P Gerber, S Rossomanno, O Krieter, A Lahr, N Wild, SV Harring, K Lechner Vanucizumab mode of action: Serial biomarkers in plasma, tumor, and skin-wound-healing biopsies Translational Oncology, 2020;14(2):100984. 2020 [PMID: 33338877] (IHC, Human) IHC Human
ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils Sulfated glycans engage the Ang-Tie pathway to regulate vascular development Nat Chem Biol, 2020;0(0):. 2020 [PMID: 33020664] (Western Blot, Human) Western Blot Human
J Gu, Y Zhang, Z Han, L Gao, J Cui, Y Sun, Y Niu, B You, CP Huang, C Chang, X Wang, S Yeh Targeting the ER&amp;beta/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC Cell Death Dis, 2020;11(5):367. 2020 [PMID: 32409702] (Western Blot, Human) Western Blot Human
J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration Sci Adv, 2019;5(2):eaau6732. 2019 [PMID: 30788433] (Immunoprecipitation, Human) Immunoprecipitation Human
T Tsujimoto, H Sudo, M Todoh, K Yamada, K Iwasaki, T Ohnishi, N Hirohama, T Nonoyama, D Ukeba, K Ura, YM Ito, N Iwasaki An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study EBioMedicine, 2018;0(0):. 2018 [PMID: 30389504] (ICC, Rabbit) ICC Rabbit
VM Leppänen, P Saharinen, K Alitalo Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28396439] (Western Blot, Human) Western Blot Human
Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P Endothelial destabilization by angiopoietin-2 via integrin beta1 activation. Nat Commun, 2015;6(0):5962. 2015 [PMID: 25635707] (ICC, Human) ICC Human
Vermi W, Micheletti A, Lonardi S, Costantini C, Calzetti F, Nascimbeni R, Bugatti M, Codazzi M, Pinter P, Schakel K, Tamassia N, Cassatella M slanDCs selectively accumulate in carcinoma-draining lymph nodes and marginate metastatic cells. Nat Commun, 2014;5(0):3029. 2014 [PMID: 24398631] (Western Blot, Human) Western Blot Human
Show All 27 Publications.

Review for Tie-2 Antibody (AF313) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF313:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous -
IF Human 01/07/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22357955
SpeciesHuman

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Recent Reviews

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Anonymous -
01/07/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol TEK
Uniprot