Angiopoietin-4 Antibody [Unconjugated]

CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Angiopoietin‑4 was detected in immersion fixed paraffin-embedded sections of human lung using 10 µg/mL Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Angiopoietin‑4 was detected in immersion fixed paraffin-embedded sections of human lung using 10 µg/mL Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

964-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB9641) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 964-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB9641) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human Angiopoietin‑4 (Catalog # 964-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB9641) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human Angiopoietin‑4 (Catalog # <a class=

• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Angiopoietin-4 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-4
  Gln23-Ile503
  Accession # Q9Y264
• Specificity: Detects human Angiopoietin‑4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) ANG-1, rhANG-2 and recombinant mouse ANG-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANGPT4
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-4 Antibody [Unconjugated]

• AGP4
• ANG3
• ANG-3
• ANG-4
• ANG4angiopoietin-3
• angiopoietin 4
• Angiopoietin-3
• Angiopoietin-4
• ANGPT4
• dJ824F16.2 (angiopoietin 4)
• MGC138181
• MGC138183

Background

GITR (glucocorticoid-induced TNF receptor superfamily-related protein, also named AITR, activation-inducible TNF receptor superfamily-related protein) and GITR ligand (GITRL) are novel members of the TNF receptor (TNFR) and TNF superfamilies (SF) that have been designated TNFRSF18 and TNFSF18, respectively. Human GITRL cDNA encodes a 177 amino acid residues type II membrane protein. The carboxy-terminal extracellular domain shows sequence identity to TNF/TNFSF2 (21%), Fas ligand/TNFSF6 (21%), TRAIL/TNFSF10 (18%), and lymphotoxin alpha /TNFSF1 (18%). GITRL is constitutively expressed in human umbilical vein endothelial cells but is not expressed in resting or stimulated T cell lines, B cell lines or peripheral blood mononuclear cells. GITR, the receptor for GITRL, is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up-regulated by antigen-receptor stimulation or by treatment with soluble anti-CD3 plus anti-CD28 or PMA plus ionomycin. Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2 and protect cells from TCR activation-induced cell death. It has been proposed that GITRL and GITR may modulate T lymphocyte functions in peripheral tissues.

1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
3. Gurney, A.L. et al. (1999) Current Biology 9:215.
4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-4 Antibody (AF964)(3)

Publications using AF964

• Brunckhorst MK, Wang H, Lu R, Yu Q Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis. Cancer Res., 2010-09-07;70(18):7283-93. 2010-09-07 [PMID: 20823154] (Western Blot, Human)
• Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-05-05;291(4):L588-95. 2006-05-05 [PMID: 16679381] (Western Blot, Primate - Papio anubis (Olive Baboon))
• Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung. Proc. Natl. Acad. Sci. U.S.A., 2005-07-11;102(29):10212-7. 2005-07-11 [PMID: 16009933] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ANGPT4
• Uniprot:
  • Q9Y264()