Angiopoietin-2 Antibody (S04-7E6) - BSA Free

Western Blot: Angiopoietin-2 Antibody (S04-7E6) [NBP3-20029] - All lanes use NBP3-20029 at 1:6000 dilution for 1 hour at room temperature.

Western Blot: Angiopoietin-2 Antibody (S04-7E6) - BSA Free [NBP3-20029] - All lanes use NBP3-20029 at 1: 6000 dilution for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S04-7E6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.3 mg/ml

Angiopoietin-2 Antibody (S04-7E6) - BSA Free Summary

• Description: Novus Biologicals Rabbit Angiopoietin-2 Antibody (S04-7E6) - BSA Free (NBP3-20029) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Angiopoietin-2 (Uniprot # O15123)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ANGPT2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Angiopoietin-2 Antibody (S04-7E6) - BSA Free

• AGPT2
• ANG2
• ANG-2
• angiopoietin 2
• Angiopoietin-2
• angiopoietin-2a
• angiopoietin-2B
• angiopoitin 2
• ANGPT2
• Tie2-ligand

Background

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANGPT2