Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free

Western Blot: Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free [NBP2-70173] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Angiopoietin 2.

Immunohistochemistry: Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free [NBP2-70173] - Analysis of Human lymphoma tissue

Immunohistochemistry: Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free [NBP2-70173] - Analysis of Human prostate tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3H7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free (NBP2-46520) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-496 of human ANGPT2(NP_001138) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANGPT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 54.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free

• AGPT2
• ANG2
• ANG-2
• angiopoietin 2
• Angiopoietin-2
• angiopoietin-2a
• angiopoietin-2B
• angiopoitin 2
• ANGPT2
• Tie2-ligand

Background

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANGPT2