Angiogenin Antibody [Unconjugated]

HAF017). A specific band was detected for Angiogenin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band was detected for Angiogenin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows human plasma and rat plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiogenin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows human plasma and rat plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="Angiogenin was detected in immersion fixed paraffin-embedded sections of rat kidney using Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

Angiogenin was detected in immersion fixed paraffin-embedded sections of rat kidney using Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="Angiogenin was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

Angiogenin was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." > HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." title="Simple Western lane view shows human plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Angiogenin at approximately 20 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." />

Simple Western lane view shows human plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Angiogenin at approximately 20 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

265-AN) was serially diluted and captured by Mouse Anti-Human Angiogenin Monoclonal Antibody (Catalog # MAB265) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 265-AN) was serially diluted and captured by Mouse Anti-Human Angiogenin Monoclonal Antibody (Catalog # MAB265) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human Angiogenin (Catalog # 265-AN) was serially diluted and captured by Mouse Anti-Human Angiogenin Monoclonal Antibody (Catalog # MAB265) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human Angiogenin (Catalog # <a class=

• Reactivity: Hu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Angiogenin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Angiogenin
  Gln25-Pro147
  Accession # Q53X86
• Specificity: Detects human Angiogenin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiogenin Antibody [Unconjugated]

• ALS9
• ANG
• Angiogenin
• angiogenin, ribonuclease, RNase A family, 5
• EC 3.1.27
• EC 3.1.27.-
• epididymis luminal protein 168
• HEL168
• MGC22466
• MGC71966
• Ribonuclease 5
• RNase 5
• RNASE5RNASE4

Background

Angiogenin was initially purified from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29 based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane. A number of other tumor, as well as normal, cell lines can also secrete Angiogenin. In addition, Angiogenin is present in normal human plasma at levels as high as 60‑120 ng/mL. Unlike other angiogenic factors such as FGF, Angiogenin is neither mitogenic nor chemotactic for vascular endothelial cells in vitro. However, Angiogenin can stimulate capillary and umbilical vein endothelial cells to produce diacylglycerol and secrete prostacyclin by phospholipase activation. Angiogenin, absorbed on plastic, can also support endothelial and fibroblast cell adhesion and spreading.

Surprisingly, Angiogenin has been found to be a member of the ribonuclease superfamily with approximately 35% sequence similarity at the amino acid level with pancreatic RNase. Angiogenin exhibits ribonucleolytic activity that is distinctly different than that of pancreatic RNase A. The ribonucleolytic activity of Angiogenin toward most RNase A substrates is much lower than that of RNase A. Nevertheless, the ribonucleolytic activity of Angiogenin is essential to its angiogenic activity since inhibition of the Angiogenin RNase activity will also abolish angiogenesis activity. Similar to several members of the RNase superfamily, Angiogenin is a cytotoxic agent that can abolish cellular protein synthesis. It has been demonstrated that Angiogenin-dependent protein synthesis inhibition can be attributed to the function of Angiogenin as a cytotoxic tRNA-specific RNAase.

A cell-surface Angiogenin binding protein has been purified and characterized. Tryptic peptide mapping and sequence analysis indicate that this binding protein is a member of the actin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiogenin Antibody (AF265)(7)

Publications using AF265

• WJ Wang, JM Hsu, YN Wang, HH Lee, H Yamaguchi, HW Liao, MC Hung An essential role of PRMT1-mediated EGFR methylation in EGFR activation by ribonuclease 5 Am J Cancer Res, 2019-01-01;9(1):180-185. 2019-01-01 [PMID: 30755821] (Western Blot, Human)
• YN Wang, HH Lee, CK Chou, WH Yang, Y Wei, CT Chen, J Yao, JL Hsu, C Zhu, H Ying, Y Ye, WJ Wang, SO Lim, W Xia, HW Ko, X Liu, CG Liu, X Wu, H Wang, D Li, LR Prakash, MH Katz, Y Kang, M Kim, JB Fleming, D Fogelman, M Javle, A Maitra, MC Hung Angiogenin/Ribonuclease 5 Is an EGFR Ligand and a Serum Biomarker for Erlotinib Sensitivity in Pancreatic Cancer Cancer Cell, 2018-03-29;33(4):752-769.e8. 2018-03-29 [PMID: 29606349] (IHC, Human)
• M Crivello, SL O'Riordan, I Woods, S Cannon, L Halang, KS Coughlan, MC Hogg, SA Lewandowsk, JHM Prehn Pleiotropic activity of systemically delivered angiogenin in the SOD1G93Amouse model Neuropharmacology, 2018-02-25;133(0):503-511. 2018-02-25 [PMID: 29486168] (IHC-Fr, Human)
• Mirel-Adrian Popa, Maria-Cristina Mihai, Alina Constantin, Viorel Şuică, Cătălin Ţucureanu, Raluca Costache et al. Dihydrotestosterone induces pro-angiogenic factors and assists homing of MSC into the cardiac tissue Journal of Molecular Endocrinology 2018-01-01 [PMID: 29247133] (Western Blot, Human)
• Selitsky S, Baran-Gale J, Honda M, Yamane D, Masaki T, Fannin E, Guerra B, Shirasaki T, Shimakami T, Kaneko S, Lanford R, Lemon S, Sethupathy P Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C. Sci Rep, 2015-01-08;5(0):7675. 2015-01-08 [PMID: 25567797] (IHC, Human)
• Burger-van Paassen N, Loonen LM, Witte-Bouma J, Korteland-van Male AM, de Bruijn AC, van der Sluis M, Lu P, Van Goudoever JB, Wells JM, Dekker J, Van Seuningen I, Renes IB Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4. PLoS ONE, 2012-06-19;7(6):e38798. 2012-06-19 [PMID: 22723890] (IHC-P, Mouse)
• Steidinger TU, Standaert DG, Yacoubian TA A neuroprotective role for angiogenin in models of Parkinson&#039;s disease. J. Neurochem., 2010-12-22;116(3):334-41. 2010-12-22 [PMID: 21091473] (ICC, Western Blot, Human, Mouse)

Bioinformatics

• Gene Symbol: ANG
• Uniprot:
  • Q53X86()