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HAF017). A specific band was detected for Angiogenin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank">
265-AN) was serially diluted and captured by Mouse Anti-Human Angiogenin Monoclonal Antibody (Catalog # MAB265) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
265-AN) was serially diluted and captured by Mouse Anti-Human Angiogenin Monoclonal Antibody (Catalog # MAB265) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">