alpha Tubulin Antibody (DM1A) - BSA Free

Images

 
Simple Western: alpha Tubulin Antibody (DM1A) [NB100-690] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed colon sections. Heat mediated antigen retrieval was performed using sodium citrate buffer for 20 min before incubating with ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of colon tissue. Sections were formalin fixed and embedded with paraffin. Sodium citrate heat mediated antigen retrieval for 20 min. Incubated ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed paraffin embedded heart sections. Used at a dilution of 1:500.
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analsis of alpha tubulin in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using alpha Tubulin antibody (dark blue). Isotype control shown in orange. An ...read more
Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa cells, green staining is alpha tubulin whereas red is DNA stained with propidium iodide.
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - GS treatment increases markers of beiging in 3T3-L1 adipocytes. GS treatment upregulates markers of beiging, including beta-3AR (C) proteins. Data presented as ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse prostate using alpha Tubulin Antibody (DM1A) at 1:200 dilution. ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin [DM1A] Antibody NB100-690AF700 (blue) and a matched isotype control (orange). Cells were ...read more
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Western blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000, Alpha tubulin molecular weight: 50 kDa)
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of alpha tubulin (molecular weight of 50 kDa) in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. ...read more
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa and COS-7 lysates. Alpha tubulin molecular weight: 50 kDa.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - IF Confocal analysis of C6 cells using alpha Tubulin antibody (NB100-690, 1:50). An Alexa Fluor 488-conjugated Goat to mouse IgG was ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of skin fibroblasts.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of embryonic fibroblasts in the anaphase portion of mitosis.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of paraffin embedded colon sections.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of small intestine tissue fixed with formalin and paraffin embedded showing cytoplasmic and cytoskeletal staining of glandular cells.
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse skeletal muscle using alpha Tubulin Antibody (DM1A) at 1:100 dilution ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse lung using alpha Tubulin Antibody (DM1A) at 1:100 dilution with ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse heart using alpha Tubulin Antibody (DM1A) at 1:100 dilution with ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on RAW 246.7 cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on SH-SY5Y cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a ...read more
Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody (DM1A) NB100-690AF488 (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690G (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin [DM1A] Antibody NB100-690AF647 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690JF646 (blue) and a matched isotype control (orange). Cells were ...read more
Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of the marine parasite Cryptocaryon irritans mouth. Large bundles of microtubules form a cytophyrigeal basket.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, Gp, Gt, Ha, Pa, Pm, Pm, Rb, Xp, YeSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready
Clone
DM1A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

alpha Tubulin Antibody (DM1A) - BSA Free Summary

Description
As the TUBA1A gene is conserved evolutionarily and is ubiquitously expressed in most eukaryotic cell lines, the Alpha tubulin antibody has been shown to be an attractive and effective choice for a loading control, detecting at approximately 50-55 kDa. Quantitative western blotting requires a loading control in order to account and adjust for the differences in the loading of samples across wells.
Immunogen
This alpha Tubulin Antibody (DM1A) was developed against native chicken brain microtubules.
Marker
Microtubule Marker
Specificity
This alpha Tubulin Antibody (DM1A) does not cross-react with beta Tubulin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50000-1:100000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunomicroscopy
  • Immunoprecipitation 1:50-1:100
  • Simple Western 1:50
  • Western Blot 1:5000
Application Notes
This alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry.
The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.

Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue; matrix was 12-230 kDa. Only 10 - 15 ul of the recommended dilution is used per data point.

This antibody is CyTOF ready.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Tubulin alpha-1B Overexpression Lysate
Publications
Read Publications using
NB100-690 in the following applications:

  • ICC
    1 publication
  • 8 publications
  • IHC
    1 publication
  • 1 publication
  • 2 publications
  • IP
    1 publication
  • SW
    1 publication
  • WB
    63 publications

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34871568) Use in Mouse reported in scientific literature (PMID:34533563). Human, mouse, rat, porcine, bovine, canine, chicken, guinea pig, Chinese hamster, monkey, avian, rabbit, Drosophila fungi and Xenopus. Yeast reactivity reported in scientific literature (PMID: 25126732). Goat reactivity reported in scientific literature (PMID:31805146). Will likely react with all mammals..

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for alpha Tubulin Antibody (DM1A) - BSA Free

  • alpha tubulin loading control
  • alpha tubulin monoclonal
  • alpha Tubulin
  • Alpha-Tubulin 3
  • B-ALPHA-1
  • DM1A anti-tubulin
  • dm1a monoclonal
  • DM1a tubulin
  • DM1A
  • FLJ25113
  • hum-a-tub1
  • hum-a-tub2
  • LIS3
  • TUBA1 monoclonal
  • TUBA1A
  • TUBA3
  • tublin
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-Alpha-1
  • tubulin monoclonal
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha Tubulin Antibody (NB100-690)(96)

We have publications tested in 14 confirmed species: Human, Mouse, Rat, Canine, Chicken, Drosophila, Fungi, Goat, Hamster, Monkey, Primate, Rabbit, Xenopus, Yeast.

We have publications tested in 8 applications: ICC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SW, WB.


Filter By Application
ICC
(1)
ICC/IF
(8)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
IP
(1)
SW
(1)
WB
(63)
All Applications
Filter By Species
Human
(35)
Mouse
(24)
Rat
(3)
Canine
(2)
Chicken
(1)
Drosophila
(1)
Fungi
(3)
Goat
(1)
Hamster
(1)
Monkey
(1)
Primate
(1)
Rabbit
(1)
Xenopus
(1)
Yeast
(2)
All Species
Showing Publications 1 - 10 of 96. Show All 96 Publications.
Publications using NB100-690 Applications Species
Ogando DG, Bonanno JA RNA sequencing uncovers alterations in corneal endothelial metabolism, pump and barrier functions of Slc4a11 KO mice Experimental eye research Dec 3 2021 [PMID: 34871568] (WB, Mouse) WB Mouse
Chou CH, Wang HK, Lin YC Et al. Bisdemethoxycurcumin Promotes Apoptosis and Inhibits the Epithelial-Mesenchymal Transition through the Inhibition of the G-Protein-Coupled Receptor 161/Mammalian Target of Rapamycin Signaling Pathway in Triple Negative Breast Cancer Cells Journal of agricultural and food chemistry Nov 23 2021 [PMID: 34813306]
Shyam R, Ogando DG, Choi M et al. Mitochondrial ROS Induced Lysosomal Dysfunction and Autophagy Impairment in an Animal Model of Congenital Hereditary Endothelial Dystrophy Investigative ophthalmology & visual science Sep 2 2021 [PMID: 34533563] (SW, Mouse) SW Mouse
Zaganjor E, Yoon H, Spinelli JB et al. SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis Cell reports Jul 13 2021 [PMID: 34260923] (WB) WB
Budikhina AS, Murugina NE, Maximchik PV et al. Interplay between NOD1 and TLR4 Receptors in Macrophages: Nonsynergistic Activation of Signaling Pathways Results in Synergistic Induction of Proinflammatory Gene Expression Journal of immunology (Baltimore, Md. : 1950) May 1 2021 [PMID: 33846227]
Cheng WC, Chang CY, Lo CC et al. Identification of theranostic factors for patients developing metastasis after surgery for early-stage lung adenocarcinoma Theranostics Jan 26 2021 [PMID: 33664854] (WB, Human) WB Human
Panera N, Meroni M, Longo M et al. The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation EBioMedicine Feb 25 2021 [PMID: 33640795] (WB, Human) WB Human
Lottering B, Lin YW Functional Characterization of Nociceptive Mechanisms Involved in Fibromyalgia and Electroacupuncture Brain research Jan 7 2021 [PMID: 33422528] (WB, Mouse) WB Mouse
Kryczka J, Sochacka E, Papiewska-Pajak I et al. Implications of ABCC4-Mediated cAMP Eflux for CRC Migration Cancers (Basel) Dec 2 2020 [PMID: 33261018] (WB, Human)

Details:
Citation using the HRP version of this antibody.
WB Human
Pinzaru AM, Kareh M, Lamm N et al. Met Mutation is a Potential Therapeutic Target for Advanced Endometrial Cancer Genes Dev Sep 28 2020 [PMID: 33122293] (WB, Human) WB Human
Show All 96 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin Antibody (NB100-690). (Showing 1 - 3 of 3 FAQs).

  1. I am interested in buying an actin or tubulin antibody for Western blot loading control. We would like to use it on rat heart samples. Please recommend us one of your actin and/or tubulin products, and add it to the NOX2 quotation.
    • NB100-690 is for Alpha Tubulin and has been validated for use in WB and Rat. This will be added to the quote along with our Actin Antibody.
  2. I am working on a dissection fixation protocol for a non model organism, and I am using DM1A alpha tubulin as a control.  I do not see any sort of Astral projections; does this convince you that it is a working stain ?
    • The staining looks a little off to my eye. What were your fixation conditions? Tubulin immunostaining can be finicky and requires particular fixation conditions for optimal labeling. Depending on your other antigenic sites, it's ideal to use either mixed aldehydes (PFA and Glutaraldehyde) with simultaneous extraction or methanol. PFA alone gives an undesirable hazy staining pattern.Some laboratories also recommend adding a calcium chelator into the fixation buffer as calcium induces depolymerization of microtubules. Lastly, be sure to run negative controls with your staining.
  3. We would like to stain cilia with an acetylated alpha tubulin antibody in our cells, but I am unsure if this antibody will be able to conclusively differentiate cilia from other structures such as spindle pole bodies. Does anyone know what acetylated alpha tubulin antibodies might bind to apart from cilia?
    • Acetylated alpha tubulin is found in relatively stable microtubules. It is best practice to use this marker together with a centrosome/centriole marker, which will stain the basal bodies at the base of the cilium. After that, it is relatively straightforward to identify the acetylated alpha tubulin signal that corresponds to the cilium.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Autophagy Research Update: What a difference a year makes!
By Christina Towers, PhD Over the last two decades the field of autophagy has exploded! Innovative techniques, comprehensive analysis and disease-relevant models have yielded basic and clinical discoveries of conseque...  Read full blog post.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut...  Read full blog post.

Make each cell count: How to assess autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Alpha Tubulin: A Fundamental Cytoskeleton Protein with Many Roles
The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Tubulins are the microtubule building block and exist as globular dimeric proteins of alpha/beta chains. ...  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

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Bioinformatics

Gene Symbol TUBA1A
Entrez