alpha Tubulin Antibody (DM1A)

Images

 
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of skin fibroblasts.
Simple Western: alpha Tubulin Antibody (DM1A) [NB100-690] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed colon sections. Heat mediated antigen retrieval was performed using sodium citrate buffer for 20 min before incubating with ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of colon tissue. Sections were formalin fixed and embedded with paraffin. Sodium citrate heat mediated antigen retrieval for 20 min. Incubated ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed paraffin embedded heart sections. Used at a dilution of 1:500.
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analsis of alpha tubulin in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using alpha Tubulin antibody (dark blue). Isotype control shown in orange. An ...read more
Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa cells, green staining is alpha tubulin whereas red is DNA stained with propidium iodide.
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa and COS-7 lysates. Alpha tubulin molecular weight: 50 kDa.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse prostate using alpha Tubulin Antibody (DM1A) at 1:200 dilution. ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690JF646 (blue) and a matched isotype control (orange). Cells were ...read more
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Western blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000, Alpha tubulin molecular weight: 50 kDa)
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of alpha tubulin (molecular weight of 50 kDa) in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - IF Confocal analysis of C6 cells using alpha Tubulin antibody (NB100-690, 1:50). An Alexa Fluor 488-conjugated Goat to mouse IgG was ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of embryonic fibroblasts in the anaphase portion of mitosis.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of paraffin embedded colon sections.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of small intestine tissue fixed with formalin and paraffin embedded showing cytoplasmic and cytoskeletal staining of glandular cells.
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse skeletal muscle using alpha Tubulin Antibody (DM1A) at 1:100 dilution ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse lung using alpha Tubulin Antibody (DM1A) at 1:100 dilution with ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse heart using alpha Tubulin Antibody (DM1A) at 1:100 dilution with ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on RAW 246.7 cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on SH-SY5Y cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a ...read more
Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on U-937 cells with alpha Tubulin Antibody (DM1A) NB100-690AF647 (blue) and a matched isotype control (orange). ...read more
Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody (DM1A) NB100-690AF488 (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690G (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin [DM1A] Antibody NB100-690AF647 (blue) and a matched isotype control (orange). Cells were ...read more
Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of the marine parasite Cryptocaryon irritans mouth. Large bundles of microtubules form a cytophyrigeal basket.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, Gp, Gt, Ha, Pa, Pm, Pm, Rb, Xp, YeSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready
Clone
DM1A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

alpha Tubulin Antibody (DM1A) Summary

Description
As the TUBA1A gene is conserved evolutionarily and is ubiquitously expressed in most eukaryotic cell lines, the Alpha tubulin antibody has been shown to be an attractive and effective choice for a loading control, detecting at approximately 50-55 kDa. Quantitative western blotting requires a loading control in order to account and adjust for the differences in the loading of samples across wells.
Immunogen
This alpha Tubulin Antibody (DM1A) was developed against native chicken brain microtubules.
Marker
Microtubule Marker
Specificity
This alpha Tubulin Antibody (DM1A) does not cross-react with beta Tubulin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Simple Western 1:50
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunomicroscopy
  • Immunoprecipitation 1:50-1:100
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
This alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry. The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

This antibody is CyTOF ready.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Tubulin alpha-1B Overexpression Lysate
Human Lung Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 14 Reviews rated 4.6
using
NB100-690 in the following applications:

Publications
Read Publications using
NB100-690 in the following applications:

Reactivity Notes

Human, mouse, rat, porcine, bovine, canine, chicken, guinea pig, Chinese hamster, monkey, avian, rabbit, Drosophila fungi and Xenopus. Yeast reactivity reported in scientific literature (PMID: 25126732). Goat reactivity reported in scientific literature (PMID:31805146). Will likely react with all mammals.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for alpha Tubulin Antibody (DM1A)

  • alpha tubulin loading control
  • alpha tubulin monoclonal
  • alpha Tubulin
  • Alpha-Tubulin 3
  • B-ALPHA-1
  • DM1A anti-tubulin
  • dm1a monoclonal
  • DM1a tubulin
  • DM1A
  • FLJ25113
  • hum-a-tub1
  • hum-a-tub2
  • LIS3
  • TUBA1 monoclonal
  • TUBA1A
  • TUBA3
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-Alpha-1
  • tubulin monoclonal
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T.,... Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-87769
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
AF7068
Species: Hu
Applications: WB, IHC, ICC
NB120-22104
Species: Hu, Mu, Rt
Applications: WB
NBP2-46250
Species: Hu, Mu, Ca, Pm
Applications: WB, IHC, IF
NB600-1081
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KO
MAB1195
Species: All-Multi
Applications: WB, Simple Western, IHC, ICC
NBP1-78162
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-02474
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-64792
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-936
Species: Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, In, Pm, Xp, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, Single-Cell Western
NBP2-43837
Species: Hu, Mu, Rt, Dr, Ze
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, Tumorsphere
NBP1-31528
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-77370
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-31004
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88607
Species: Hu
Applications: WB, IHC, IHC-P
H00002316-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA

Publications for alpha Tubulin Antibody (NB100-690)(76)

We have publications tested in 14 confirmed species: Human, Mouse, Rat, Canine, Chicken, Drosophila, Fungi, Goat, Hamster, Monkey, Primate, Rabbit, Xenopus, Yeast.

We have publications tested in 5 applications: ICC/IF, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(8)
IHC-Fr
(1)
IHC-P
(2)
IP
(1)
WB
(51)
All Applications
Filter By Species
Human
(28)
Mouse
(16)
Rat
(3)
Canine
(2)
Chicken
(1)
Drosophila
(1)
Fungi
(3)
Goat
(1)
Hamster
(1)
Monkey
(1)
Primate
(1)
Rabbit
(1)
Xenopus
(1)
Yeast
(2)
All Species
Showing Publications 1 - 10 of 76. Show All 76 Publications.
Publications using NB100-690 Applications Species
Yang C, Fan M, Miao C et al. Engineering Chimeric Antigen Receptor T cells Against Immune Checkpoint Inhibitors PD-1/PD-L1 for Treating Pancreatic Cancer Molecular Therapy - Oncolytics May 1 2020 [PMID: 32637575] (WB, Human) WB Human
Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J. Clin. Invest. May 19 2020 [PMID: 32427586] (WB, Human) WB Human
Kaid C, Madi RADS, Astray R et al. Safety, Tumor Reduction, and Clinical Impact of Zika Virus Injection in Dogs with Advanced-Stage Brain Tumors Mol. Ther. Mar 10 2020 [PMID: 32220305] (IHC-P, Canine) IHC-P Canine
Jaworski Jp, Urrutia M, Dascal E et Al. C-C motif chemokine receptor 2 as a novel intermediate in the ovulatory cascade Mol. Hum. Reprod. Mar 11 2020 [PMID: 32159806]
TRPM7 contributes to progressive nephropathy Sci Rep Feb 11 2020 [PMID: 32047249] (WB, Mouse) WB Mouse
Chou CW, Lin CH, Hsiao TH et al. Therapeutic effects of statins against lung adenocarcinoma via p53 mutant-mediated apoptosis Sci Rep Dec 31 2019 [PMID: 31892709] (WB, Human) WB Human
Yamada Y, Liao GR, Tseng CY, et al. Establishment and characterization of transformed goat primary cells by expression of simian virus 40 large T antigen for orf virus propagations PLoS ONE Dec 5 2019 [PMID: 31805146] (WB, Human, Primate, Goat) WB Human, Primate, Goat
Doyle TB, Muntean BS, Ejendal KF et al. Identification of Novel Adenylyl Cyclase 5 (AC5) Signaling Networks in D1 and D2 Medium Spiny Neurons using Bimolecular Fluorescence Complementation Screening Cells. Nov 19 2019 [PMID: 31752385] (WB, Human) WB Human
Murray JR, de la Vega L, Hayes JD et al. Induction of the Antioxidant Response by the Transcription Factor NRF2 Increases Bioactivation of the Mutagenic Air Pollutant 3-Nitrobenzanthrone in Human Lung Cells Chem. Res. Toxicol. Nov 20 2019 [PMID: 31746589]
Seale LA, Khadka VS, Menor M et al. Combined Omics Reveals That Disruption of the Selenocysteine Lyase Gene Affects Amino Acid Pathways in Mice Nutrients 2019 Oct 26 [PMID: 31717805]
Show All 76 Publications.

Reviews for alpha Tubulin Antibody (NB100-690) (14) 4.614

Average Rating: 4.6
(Based on 14 reviews)
We have 14 reviews tested in 4 species: Human, Mouse, Human and Mouse, Other.

Reviews using NB100-690:
Filter by Applications
WB
(12)
ICC
(2)
All Applications
Filter by Species
Human
(10)
Mouse
(2)
Human and Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot alpha Tubulin NB100-690
Enlarge
reviewed by:
Anonymous
WB Human 05/18/2020
View

Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotA-4
Western Blot alpha Tubulin NB100-690
Enlarge
reviewed by:
Anonymous
WB Human 10/22/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
LotA-4
Western Blot alpha Tubulin NB100-690
Enlarge
reviewed by:
Jun Hamazaki
WB Human and Mouse 06/29/2017
View

Summary

ApplicationWestern Blot
Sample Tested293T cell lysate,nih3t3 cell lysate
SpeciesHuman and Mouse
Lote-3
Western Blot alpha Tubulin NB100-690
Enlarge
reviewed by:
Emily Ledgerwood
WB Other 07/15/2016
View

Summary

ApplicationWestern Blot
Sample TestedAfrican Green Monkey Kidney (CV-1) whole cell lysate
SpeciesOther
LotE-1

Blocking

Blocking Details1 hour in 5% milk/TBST

Primary Anitbody

Dilution Ratioalpha tubulin NB100-690 1:5000 in 5% milk/TBST overnight at 4 degrees. This is a membrane that was stripped and reprobed.

Secondary Antibody

Secondary DescriptionDonkey anti-mouse HRP
Secondary Manufacturer Cat#715-035-150
Secondary Concentration1:10000

Details

Detection NotesExposed on a LiCor C-Digit using Advansta WesternBright Quantum

Comments

CommentsThis is a fantastic antibody for alpha-tubulin that I have been using for years. For this review, I opted to submit an image of a PVDF membrane that was originally probed for a different primary, stripped using LiCor's Western Blot Stripping Buffer according to manufacturer's instructions and then reprobed with this alpha-tubulin antibody. To top it off, I am imaging on a C-digit which is the cheapest imager on the market. This antibody is extremely reliable and well worth the money.
Western Blot alpha Tubulin NB100-690
Enlarge
reviewed by:
Anony- mous
WB Human 06/03/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000).
FileView PDF
Immunocytochemistry alpha Tubulin NB100-690
Enlarge
reviewed by:
Anony- mous
ICC Human 06/03/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of C6 cells using alpha Tubulin antibody (NB100-690, 1:50). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF
Immunocytochemistry alpha Tubulin NB100-690
Enlarge
reviewed by:
Samantha Garcia
ICC Human 11/06/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedMDA-MB-231 cells
SpeciesHuman
LotA2

Blocking

Blocking DetailsPBS with 2% BSA

Primary Anitbody

Dilution Ratio1:2000 in blocking buffer

Details

Fixation DetailsMDA-MB-231 cells were fixed in 4% PFA. Glutaraldehyde fixation also worked.
Western Blot alpha Tubulin NB100-690
Enlarge
reviewed by:
Anonymous
WB Mouse 01/06/2012
View

Summary

ApplicationWestern Blot
Sample Testedmouse lung tissue
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 100ug FBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL, Exposure Time: 1 minute, Specific Bands: 51 kDa, Non-Specific Bands: 50-55 kDa
 
reviewed by:
Anonymous
WB Mouse 12/05/2011
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesMouse
CommentsThe signal is strong and clear.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 2 hours

Secondary Antibody

Secondary DescriptionSecondary Ab: mouse HRP

Details

Detection NotesDetection Method: ECL with X-ray film

Comments

CommentsThe signal is strong and clear.
 
reviewed by:
Anonymous
WB Human 11/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsWorked well

Blocking

Blocking DetailsBlocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: mouse

Details

Detection NotesDetection Method: HRP

Comments

CommentsWorked well
 
reviewed by:
Anonymous
WB Human 11/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman cell
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse

Details

Detection NotesDetection Method: HRP
 
reviewed by:
Anonymous
WB Human 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit

Details

Detection NotesDetection Method: Western Lighting plus ECL
 
reviewed by:
Anonymous
WB Human 11/18/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman cell
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP

Details

Detection NotesDetection Method: chemiluminescence system
 
reviewed by:
Anonymous
WB Human 10/31/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGreat performance! Clear band in signal

Blocking

Blocking DetailsBlocking Buffer: 5% Skim Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP conjugated anti-mouse

Details

Detection NotesDetection Method: Chemiluminescence

Comments

CommentsGreat performance! Clear band in signal

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin Antibody (NB100-690). (Showing 1 - 3 of 3 FAQs).

  1. I am interested in buying an actin or tubulin antibody for Western blot loading control. We would like to use it on rat heart samples. Please recommend us one of your actin and/or tubulin products, and add it to the NOX2 quotation.
    • NB100-690 is for Alpha Tubulin and has been validated for use in WB and Rat. This will be added to the quote along with our Actin Antibody.
  2. I am working on a dissection fixation protocol for a non model organism, and I am using DM1A alpha tubulin as a control.  I do not see any sort of Astral projections; does this convince you that it is a working stain ?
    • The staining looks a little off to my eye. What were your fixation conditions? Tubulin immunostaining can be finicky and requires particular fixation conditions for optimal labeling. Depending on your other antigenic sites, it's ideal to use either mixed aldehydes (PFA and Glutaraldehyde) with simultaneous extraction or methanol. PFA alone gives an undesirable hazy staining pattern.Some laboratories also recommend adding a calcium chelator into the fixation buffer as calcium induces depolymerization of microtubules. Lastly, be sure to run negative controls with your staining.
  3. We would like to stain cilia with an acetylated alpha tubulin antibody in our cells, but I am unsure if this antibody will be able to conclusively differentiate cilia from other structures such as spindle pole bodies. Does anyone know what acetylated alpha tubulin antibodies might bind to apart from cilia?
    • Acetylated alpha tubulin is found in relatively stable microtubules. It is best practice to use this marker together with a centrosome/centriole marker, which will stain the basal bodies at the base of the cilium. After that, it is relatively straightforward to identify the acetylated alpha tubulin signal that corresponds to the cilium.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-690AF350
Alexa Fluor 405 NB100-690AF405
Alexa Fluor 488 NB100-690AF488
Alexa Fluor 532 NB100-690AF532
Alexa Fluor 594 NB100-690AF594
Alexa Fluor 647 NB100-690AF647
Alexa Fluor 700 NB100-690AF700
Alexa Fluor 750 NB100-690AF750
Allophycocyanin NB100-690APC
Biotin NB100-690B
DyLight 350 NB100-690UV
DyLight 405 NB100-690V
DyLight 488 NB100-690G
DyLight 550 NB100-690R
DyLight 650 NB100-690C
DyLight 680 NB100-690FR
DyLight 755 NB100-690IR
FITC NB100-690F
HRP NB100-690H
Janelia Fluor 549 NB100-690JF549
Janelia Fluor 646 NB100-690JF646
PE NB100-690PE
PerCP NB100-690PCP

Additional alpha Tubulin Products

Bioinformatics Tool for alpha Tubulin Antibody (NB100-690)

Discover related pathways, diseases and genes to alpha Tubulin Antibody (NB100-690). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for alpha Tubulin Antibody (NB100-690)

Discover more about diseases related to alpha Tubulin Antibody (NB100-690).
 

Pathways for alpha Tubulin Antibody (NB100-690)

View related products by pathway.

PTMs for alpha Tubulin Antibody (NB100-690)

Learn more about PTMs related to alpha Tubulin Antibody (NB100-690).
 

Research Areas for alpha Tubulin Antibody (NB100-690)

Find related products by research area.

Blogs on alpha Tubulin.

Autophagy Research Update: What a difference a year makes!
By Christina Towers, PhD Over the last two decades the field of autophagy has exploded! Innovative techniques, comprehensive analysis and disease-relevant models have yielded basic and clinical discoveries of conseque...  Read full blog post.


  Read full blog post.


  Read full blog post.


  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Alpha Tubulin: A Fundamental Cytoskeleton Protein with Many Roles
The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Tubulins are the microtubule building block and exist as globular dimeric proteins of alpha/beta chains....  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.6
5
9
4
5
3
0
2
0
1
0

Anonymous
05/18/2020
Application: WB
Species: Human

Anonymous
10/22/2018
Application: WB
Species: Human

Jun Hamazaki
06/29/2017
Application: WB
Species: Human and Mouse

Bioinformatics

Gene Symbol TUBA1A
Entrez