alpha Tubulin Antibody (DM1A) - BSA Free

Simple Western: alpha Tubulin Antibody (DM1A) [NB100-690] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Alpha tubulin molecular weight: 50 kDa.

Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed colon sections. Heat mediated antigen retrieval was performed using sodium citrate buffer for 20 min before incubating with primary antibody at a 0.5ug/ml dilution for 15 min at RT.

Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of colon tissue. Sections were formalin fixed and embedded with paraffin. Sodium citrate heat mediated antigen retrieval for 20 min. Incubated with primary antibody for 15 min at a 5 ug/ml concentration. Corner image is staining with secondary only.

Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed paraffin embedded heart sections. Used at a dilution of 1:500.

Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analsis of alpha tubulin in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. MDBK; Lane 9. MDCK

Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using alpha Tubulin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa cells, green staining is alpha tubulin whereas red is DNA stained with propidium iodide.

Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - GS treatment increases markers of beiging in 3T3-L1 adipocytes. GS treatment upregulates markers of beiging, including beta-3AR (C) proteins. Data presented as mean +/- SEM from n = 4 replicates per group. * p < 0.05, *** p < 0.001 vs. control. Abbreviations: beta-3 adrenergic receptor (beta-3AR). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2305-6320/6/1/22), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) - BSA Free [NB100-690] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with alpha Tubulin Antibody [DM1A] conjugated to Alexa Fluor 647 (NB100-690AF647) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse prostate using alpha Tubulin Antibody (DM1A) at 1:200 dilution. The signal was developed using HRP labelled secondary and DAB reagent which followed counterstaining with hematoxylin. The antibody generated a specific cytoplasmic/cytoskeletal staining in the prostate epithelial cells.

Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin [DM1A] Antibody NB100-690AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Western blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000, Alpha tubulin molecular weight: 50 kDa)

Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of alpha tubulin (molecular weight of 50 kDa) in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. MDBK; Lane 9. MDCK

Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa and COS-7 lysates. Alpha tubulin molecular weight: 50 kDa.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - IF Confocal analysis of C6 cells using alpha Tubulin antibody (NB100-690, 1:50). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-alpha Tubulin (DM1A) (NB100-690) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of skin fibroblasts.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of embryonic fibroblasts in the anaphase portion of mitosis.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-alpha Tubulin Antibody [DM1A] conjugated to Alexa Fluor 488 (NB100-690AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-alpha Tubulin Antibody [DM1A] conjugated to Alexa Fluor 488 (NB100-690AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-alpha Tubulin Antibody [DM1A] conjugated to Alexa Fluor 488 (NB100-690AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with alpha Tubulin Antibody [DM1A] conjugated to Janelia Fluor 549 (NB100-690JF549) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with alpha Tubulin Antibody [DM1A] conjugated to Janelia Fluor 549 (NB100-690JF549) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of paraffin embedded colon sections.

Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of small intestine tissue fixed with formalin and paraffin embedded showing cytoplasmic and cytoskeletal staining of glandular cells.

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse skeletal muscle using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong cytoplasmic signal in the muscle cells with cytoplasmic-nuclear signal in the endothelial cells.

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse lung using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated chunks of cytoplasmic signal in the alveolar and bronchiolar epithelial cells.

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse heart using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong and specific cytoplasmic signal in the muscle cells.

Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on RAW 246.7 cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized wi

Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on SH-SY5Y cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with

Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody (DM1A) NB100-690AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image from the Alexa Fluor 488 version of this antibody.

Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin [DM1A] Antibody NB100-690AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690JF646 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Janelia Fluor 646.

Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of the marine parasite Cryptocaryon irritans mouth. Large bundles of microtubules form a cytophyrigeal basket.

• Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, Gp, Gt, Ha, Pa, Pm, Pm, Rb, Xp, Ye
• Applications: WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready
• Clone: DM1A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

alpha Tubulin Antibody (DM1A) - BSA Free Summary

• Description: As the TUBA1A gene is conserved evolutionarily and is ubiquitously expressed in most eukaryotic cell lines, the Alpha tubulin antibody has been shown to be an attractive and effective choice for a loading control, detecting at approximately 50-55 kDa. Quantitative western blotting requires a loading control in order to account and adjust for the differences in the loading of samples across wells.
• Immunogen: This alpha Tubulin Antibody (DM1A) was developed against native chicken brain microtubules.
• Marker: Microtubule Marker
• Specificity: This alpha Tubulin Antibody (DM1A) does not cross-react with beta Tubulin.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBA1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50000-1:100000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunomicroscopy
  • Immunoprecipitation 1:50-1:100
  • Simple Western 1:50
  • Western Blot 1:5000
• Application Notes: This alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry.
  The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.
  
  Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue; matrix was 12-230 kDa. Only 10 - 15 ul of the recommended dilution is used per data point.
  
  This antibody is CyTOF ready.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Tubulin alpha-1B Overexpression Lysate

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34871568) Use in Mouse reported in scientific literature (PMID:34533563). Human, mouse, rat, porcine, bovine, canine, chicken, guinea pig, Chinese hamster, monkey, avian, rabbit, Drosophila fungi and Xenopus. Yeast reactivity reported in scientific literature (PMID: 25126732). Goat reactivity reported in scientific literature (PMID:31805146). Will likely react with all mammals..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for alpha Tubulin Antibody (DM1A) - BSA Free

• alpha tubulin loading control
• alpha tubulin monoclonal
• alpha Tubulin
• Alpha-Tubulin 3
• B-ALPHA-1
• DM1A anti-tubulin
• dm1a monoclonal
• DM1a tubulin
• DM1A
• FLJ25113
• hum-a-tub1
• hum-a-tub2
• LIS3
• TUBA1 monoclonal
• TUBA1A
• TUBA3
• tublin
• tubulin alpha-1A chain
• Tubulin alpha-3 chain
• Tubulin B-Alpha-1
• tubulin monoclonal
• tubulin, alpha 1a
• tubulin, alpha 3
• tubulin, alpha, brain-specific

Background

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha Tubulin Antibody (NB100-690)(112)

Publications using NB100-690

• Matsuda S, Revandkar A, Dubash TD et al. TGF-? in the microenvironment induces a physiologically occurring immune-suppressive senescent state Cell reports 2023-02-22 [PMID: 36821441] (ICC/IF, Human)
• Fazel M, Wester MJ, Schodt DJ et al. High-precision estimation of emitter positions using Bayesian grouping of localizations Nature communications 2022-11-22 [PMID: 36418347]
• Kryczka J, Kryczka J, Janczewski L Et al. Isothiocyanates (ITCs) 1-(Isothiocyanatomethyl)-4-phenylbenzene and 1-Isothiocyanato-3,5-bis(trifluoromethyl)benzene-Aldehyde Dehydrogenase (ALDH) Inhibitors, Decreases Cisplatin Tolerance and Migratory Ability of NSCLC Int J Mol Sci 2022-08-12 [PMID: 35955773] (WB, Human)
  
  Details:
  Citation using the HRP version of this antibody.
• Shyam R, Ogando D, Kim E Et al. Rescue of the CHED Mouse Model by AAV-mediated Slc4a11 Replacement Ophthalmology Science 2021-11-01 (WB, Mouse)
• Paulukinas RD, Penning TM Insulin-Induced AKR1C3 Induces Fatty Acid Synthase in a Model of Human PCOS Adipocytes Endocrinology 2023-02-17 [PMID: 36799021] (WB)
• Zadeh CO, Huggins JR, Sarmah D et al. Mesowestern Blot: Simultaneous Analysis of Hundreds of Submicroliter Lysates ACS omega 2022-08-23 [PMID: 36033686]
• Crudele A, Smeriglio A, Ingegneri M et al. Hydroxytyrosol Recovers SARS-CoV-2-PLpro-Dependent Impairment of Interferon Related Genes in Polarized Human Airway, Intestinal and Liver Epithelial Cells Antioxidants 2022-07-27 [PMID: 36009185] (Simple Western, Human)
• Volkova EI, Dorogova NV, Andreyenkov OV et al. New Mutations in the 5' Region of the Notch Gene Affect Drosophila melanogaster Oogenesis Journal of developmental biology 2022-08-09 [PMID: 35997396] (WB, Drosophila)
  
  Details:
  Dilution used for WB 1:5000
• Kaczynski TJ, Au ED, Farkas MH Exploring the lncRNA localization landscape within the retinal pigment epithelium under normal and stress conditions BMC genomics 2022-07-26 [PMID: 35883037] (WB, Human)
• Azimova D, Herrera N, Duvenage L et al. Cbp1, a fungal virulence factor under positive selection, forms an effector complex that drives macrophage lysis PLoS pathogens 2022-06-22 [PMID: 35731824] (WB)
• Niu W, Spradling AC Mouse oocytes develop in cysts with the help of nurse cells Cell 2022-05-20 [PMID: 35623357] (ICC/IF, Mouse)
  
  Details:
  Stained cultered mouse ovaries. Lot E-4
• Woo C, Allawzi A, Clark N et al. Inhaled Delivery of a Lipid Nanoparticle Encapsulated Messenger RNA Encoding a Ciliary Protein for the Treatment of Primary Ciliary Dyskinesia SSRN Electronic Journal 2022-02-19 [PMID: 35613658] (FLOW, Mouse)
• Zhou M, Zhou S, Han K et al. Th1 immune maturation effects of Nocardia rubra cell-wall skeleton via PI3K/Akt/PAX8 regulatory axis Science progress 2022-04-27 [PMID: 35473474] (WB, Mouse)
• Jhan JH, Hsu WC, Lee YC et al. MicroRNA-375-3p Suppresses Upper Tract Urothelial Carcinoma Cell Migration and Invasion via Targeting Derlin-1 Cancers 2022-02-10 [PMID: 35205628] (WB, Human)
• Ogando DG, Bonanno JA RNA sequencing uncovers alterations in corneal endothelial metabolism, pump and barrier functions of Slc4a11 KO mice Experimental eye research 2021-12-03 [PMID: 34871568] (WB, Mouse)
• Chou CH, Wang HK, Lin YC Et al. Bisdemethoxycurcumin Promotes Apoptosis and Inhibits the Epithelial-Mesenchymal Transition through the Inhibition of the G-Protein-Coupled Receptor 161/Mammalian Target of Rapamycin Signaling Pathway in Triple Negative Breast Cancer Cells Journal of agricultural and food chemistry 2021-11-23 [PMID: 34813306]
• Chow S, Hsu C, Yang C, Meir Y A non-transcriptional function of YAP physically interacts with a-tubulin to stabilize the mitotic spindle and midbody structures through gaining the acetyl-a-tubulin in lung cancer cell Research Square 2022-10-06 (WB, Human)
• Shyam R, Ogando DG, Choi M et al. Mitochondrial ROS Induced Lysosomal Dysfunction and Autophagy Impairment in an Animal Model of Congenital Hereditary Endothelial Dystrophy Investigative ophthalmology & visual science 2021-09-02 [PMID: 34533563] (SW, Mouse)
• Cooney AL, Thurman AL, McCray PB Jr Et al. Lentiviral vectors transduce lung stem cells without disrupting plasticity Mol Ther Nucleic Acids 2021-08-30 [PMID: 34458011]
  
  Details:
  Citation using the Alexa Fluor 405 version of this antibody.
• Zaganjor E, Yoon H, Spinelli JB et al. SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis Cell reports 2021-07-13 [PMID: 34260923] (WB)
• Lai J, Wu D, Wu C et al. Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways iScience 2021-06-18 [PMID: 34142025]
• Budikhina AS, Murugina NE, Maximchik PV et al. Interplay between NOD1 and TLR4 Receptors in Macrophages: Nonsynergistic Activation of Signaling Pathways Results in Synergistic Induction of Proinflammatory Gene Expression Journal of immunology (Baltimore, Md. : 1950) 2021-05-01 [PMID: 33846227]
• Cheng WC, Chang CY, Lo CC et al. Identification of theranostic factors for patients developing metastasis after surgery for early-stage lung adenocarcinoma Theranostics 2021-01-26 [PMID: 33664854] (WB, Human)
• Panera N, Meroni M, Longo M et al. The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation EBioMedicine 2021-02-25 [PMID: 33640795] (WB, Human)
• Hotta T, Haynes S, Blasius T et al. Parthenolide Destabilizes Microtubules by Covalently Modifying Tubulin Curr Biol 2021-01-22 [PMID: 33482110]
• Lottering B, Lin YW Functional Characterization of Nociceptive Mechanisms Involved in Fibromyalgia and Electroacupuncture Brain research 2021-01-07 [PMID: 33422528] (WB, Mouse)
• Kryczka J, Sochacka E, Papiewska-Pajak I et al. Implications of ABCC4-Mediated cAMP Eflux for CRC Migration Cancers (Basel) 2020-12-02 [PMID: 33261018] (WB, Human)
  
  Details:
  Citation using the HRP version of this antibody.
• Pinzaru AM, Kareh M, Lamm N et al. Met Mutation is a Potential Therapeutic Target for Advanced Endometrial Cancer Genes Dev 2020-09-28 [PMID: 33122293] (WB, Human)
• Kita A, Fujiya M, Konishi H et al. Probiotic derived ferrichrome inhibits the growth of refractory pancreatic cancer cells Int. J. Oncol. 2020-09-01 [PMID: 32705165] (ICC, Human)
• Yang C, Fan M, Miao C et al. Engineering Chimeric Antigen Receptor T cells Against Immune Checkpoint Inhibitors PD-1/PD-L1 for Treating Pancreatic Cancer Molecular Therapy - Oncolytics 2020-05-01 [PMID: 32637575] (WB, Human)
• Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J Clin Invest. 2020-05-19 [PMID: 32427586] (WB, Human)
• Kaid C, Madi RADS, Astray R et al. Safety, Tumor Reduction, and Clinical Impact of Zika Virus Injection in Dogs with Advanced-Stage Brain Tumors Mol. Ther. 2020-03-10 [PMID: 32220305] (IHC-P, Canine)
• Jaworski Jp, Urrutia M, Dascal E et Al. C-C motif chemokine receptor 2 as a novel intermediate in the ovulatory cascade Mol. Hum. Reprod. 2020-03-11 [PMID: 32159806]
• TRPM7 contributes to progressive nephropathy Sci Rep 2020-02-11 [PMID: 32047249] (WB, Mouse)
• Chou CW, Lin CH, Hsiao TH et al. Therapeutic effects of statins against lung adenocarcinoma via p53 mutant-mediated apoptosis Sci Rep 2019-12-31 [PMID: 31892709] (WB, Human)
• Doyle TB, Hayes MP, Chen DH et al. Functional characterization of AC5 gain-of-function variants: Impact on the molecular basis of ADCY5-related dyskinesia Biochem. Pharmacol. 2019-02-14 [PMID: 30772269] (WB, Human)
• Assa D Corticostriatal Projections Relying on GABA Levels Mediate Exercise-Induced Functional Recovery in Cerebral Ischemic Mice Thesis 2022-01-01 (WB, Fungi)
  
  Details:
  Dilution: 1:1000
• Rucker HM Amslinger S. Characterization of the anti-inflammatory activity of enones based on the evaluation of their heme oxygenase-1 and inducible NO synthase activity Thesis 2015-01-01 (WB, Mouse)
• Yamada Y, Liao GR, Tseng CY, et al. Establishment and characterization of transformed goat primary cells by expression of simian virus 40 large T antigen for orf virus propagations PLoS ONE 2019-12-05 [PMID: 31805146] (WB, Human, Goat, Primate)
• Doyle TB, Muntean BS, Ejendal KF et al. Identification of Novel Adenylyl Cyclase 5 (AC5) Signaling Networks in D1 and D2 Medium Spiny Neurons using Bimolecular Fluorescence Complementation Screening Cells. 2019-11-19 [PMID: 31752385] (WB, Human)
• Murray JR, de la Vega L, Hayes JD et al. Induction of the Antioxidant Response by the Transcription Factor NRF2 Increases Bioactivation of the Mutagenic Air Pollutant 3-Nitrobenzanthrone in Human Lung Cells Chem. Res. Toxicol. 2019-11-20 [PMID: 31746589]
• Seale LA, Khadka VS, Menor M et al. Combined Omics Reveals That Disruption of the Selenocysteine Lyase Gene Affects Amino Acid Pathways in Mice Nutrients 2019-10-26 [PMID: 31717805]
• Lee S, Eyer J, Letournel F et al. Neurofilaments form flexible bundles during neuritogenesis in culture and in mature axons in situ J. Neurosci. Res. 2019-07-15 [PMID: 31304612] (ICC/IF, Chicken, Mouse, Canine)
• Tajima K, Matsuda S, Yae T et al. SETD1A protects from senescence through regulation of the mitotic gene expression program Nat Commun 2019-06-28 [PMID: 31253781] (ICC/IF, Human)
• Lutz MM th, Worth MP, Hinchman MM et al. Mammalian orthoreovirus Infection is Enhanced in Cells Pre-Treated with Sodium Arsenite Viruses. 2019-06-17 [PMID: 31216693] (WB, Monkey)
• Miller, CN;Samuels, JS;Azhar, Y;Parmar, A;Shashidharamurthy, R;Rayalam, S; Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages Medicines (Basel) 2019-01-31 [PMID: 30709026] (WB, Mouse)
• Kandel MB, Yamamoto S, Midorikawa R et al. N-glycosylation of the AMPA-type glutamate receptor regulates cell surface expression and tetramer formation affecting channel function J. Neurochem. 2018-08-09 [PMID: 30092607] (WB, Human)
• Nel M, Joubert AM, Dohle W et al. Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther. 2018-06-25 [PMID: 29983544] (ICC/IF, Human)
• Lin CY, Yang ST, Shen SC et al. Serum amyloid A1 in combination with integrin aVB3 increases glioblastoma cells mobility and progression Mol Oncol 2018-05-01 [PMID: 29603594] (WB, Human)
• Stock V, Sutter A, Raschke M, Queisser N. A tripartite mode of action approach for investigating the impact of aneugens on tubulin polymerization. Environ Mol Mutagen. [PMID: 29205516] (ICC/IF, Hamster)
• Rodrigues B A, Kuga G K et al. Overexpression of Mitogen-activated protein kinase phosphatase-3 (MKP-3) reduces FoxO1 phosphorylation in mice hypothalamus. Neurosci Lett 2017-10-17 [PMID: 28866049] (Mouse)
• Tomoshige S, Kobayashi Y, Hosoba K et al. Cytoskeleton-related regulation of primary cilia shortening mediated by melanin-concentrating hormone receptor 1 Gen. Comp. Endocrinol. 2017-08-22 [PMID: 28842217] (WB, Human)
• Zhang M, Yu Q, Liu Z et al. UBX domain-containing proteins are involved in lipid homeostasis and stress responses in Pichia pastoris Int. J. Biochem. Cell Biol. 2017-08-12 [PMID: 28807601] (WB, Yeast)
• Vent-Schmidt RYJ, Wen RH, Zong Z et al. Opposing Effects of Valproic Acid Treatment Mediated by Histone Deacetylase Inhibitor Activity in Four Transgenic X. laevis Models of Retinitis Pigmentosa J Neurosci. 2017-01-24 [PMID: 28490005] (WB, Xenopus)
• Chen PC, Hsieh MH, Wen-ShuoKuo P et al. Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis. J. Allergy Clin. Immunol. 2017-04-12 [PMID: 28412394] (WB)
• Sidoli M, Musner N, Silvestri N et al. Ablation of Perk in Schwann Cells Improves Myelination in the S63del Charcot-Marie-Tooth 1B Mouse. J. Neurosci. 2016-11-02 [PMID: 27807175] (WB, Mouse)
• Rueli RH, Torres DJ, Dewing AS et al. Selenoprotein S Reduces Endoplasmic Reticulum Stress-Induced Phosphorylation of Tau: Potential Role in Selenate Mitigation of Tau Pathology. J. Alzheimers Dis. 2016-10-11 [PMID: 27802219]
• Nakamura M, Nishikawa J et al. Decitabine inhibits tumor cell proliferation and up-regulates e-cadherin expression in Epstein-Barr virus-associated gastric cancer. J Med Virol 2017-01-03 [PMID: 27430892] (IF/IHC, Human)
• Chang CF, Hsu LS, Weng CY et al. N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability. Int J Mol Sci 2016-06-18 [PMID: 27314333] (WB)
• Macedo CG, Napimoga MH, Rocha-Neto LM et al. The role of endogenous opioid peptides in the antinociceptive effect of 15-deoxy(delta12,14)-prostaglandin J2 in the temporomandibular joint. Prostaglandins Leukot. Essent. Fatty Acids [PMID: 27255640] (WB)
• Park HS, Jang JE, Ko MS et al. Statins Increase Mitochondrial and Peroxisomal Fatty Acid Oxidation in the Liver and Prevent Non-Alcoholic Steatohepatitis in Mice. Diabetes Metab J. 2016-04-21 [PMID: 27098507] (WB, Mouse)
• Tu DG, Yu Y, Lee CH et al. Hinokitiol inhibits vasculogenic mimicry activity of breast cancer stem/progenitor cells through proteasome-mediated degradation of epidermal growth factor receptor. Oncol Lett 2016-04-01 [PMID: 27073579] (WB)
• Wang TK, Lin YM, Lo CM et al. Oncogenic roles of carbonic anhydrase 8 in human osteosarcoma cells. Tumour Biol. 2015-12-28 [PMID: 26711783] (WB, Human)
  
  Details:
  beta-Actin antibody (clone AC-15) was used for WB assays in experiments involving human osteosarcoma cell lines MG-63, U-2 OS, HOS and 143B and human embryonic kidney cell line HEK293.
• Farnsworth NL, Walter RL, Hemmati A et al. Low Level Pro-Inflammatory Cytokines Decrease Connexin36 Gap Junction Coupling in Mouse and Human Islets through Nitric Oxide Mediated Protein Kinase Cdelta. J. Biol. Chem. 2015-12-14 [PMID: 26668311] (WB, Mouse)
  
  Details:
  alpha Tubulin antibody (clone DM1A) used for WB on mouse islets at a dilution of 1:5,000. alpha Tubulin antibody was used as a loading control (Fig. 6D).
• Pitts MW, Kremer PM, Hashimoto AC et al. Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease. J. Neurosci. 2015-11-18 [PMID: 26586820] (WB, Mouse)
  
  Details:
  Alpha Tubulin antibody used for WB as a loading control at a dilution of 1:5000 on mouse brainstem and whole-brain samples (Figure 6A).
• Alleaume-Butaux A, Nicot S, Pietri M et al. Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation. PLoS Pathog. 2015-08-01 [PMID: 26241960] (WB, ICC/IF, Mouse)
• Seale LA, Gilman CL, Hashimoto AC et al. Diet-induced obesity in the selenocysteine lyase knockout mouse Antioxid. Redox Signal. 2015-07-20 [PMID: 26192035]
• Zhang W, Ogando DG, Bonanno JA, Obukhov AG. Human SLC4A11 is a Novel NH3: H+ Co-transporter J. Biol. Chem. 2015-05-27 [PMID: 26018076] (WB, Human)
• Bachman MJ, Foltz K, Lynch JM et al. Using cytochrome P4501A1 expression in liver and blubber to understand effects of persistent organic pollutant exposure in stranded pacific island cetaceans Environ. Toxicol. Chem. 2015-04-18 [PMID: 25892359] (WB)
• Sulaiman MK, Chu Z, Blanco VM et al. SapC-DOPS nanovesicles induce Smac- and Bax-dependent apoptosis through mitochondrial activation in neuroblastomas Mol. Cancer 2015-04-19 [PMID: 25889084] (WB, Human)
• Stander XX, Stander BA, Joubert AM. Synergistic Anticancer Potential of Dichloroacetate and Estradiol Analogue Exerting their Effect via ROS-JNK-Bcl-2-Mediated Signalling Pathways Cell. Physiol. Biochem. 2015-03-31 [PMID: 25791820] (WB, Human)
• Munoz-Saez E, de Munck Garcia E, Arahuetes Portero RM et al. Neuroprotective role of sphingosine-1-phosphate in L-BMAA treated neuroblastoma cells (SH-SY5Y). Neurosci. Lett. 2015-03-10 [PMID: 25769802] (WB, Human)
  
  Details:
  Alpha-Tubulin antibody (DM1A) used for WB as a loading control on total extract and nucleus from SH-SY5Y cells (Fig. 3 & 4).
• Eriksen AB, Torgersen ML, Holm KL et al. Retinoic acid-induced IgG production in TLR-activated human primary B cells involves ULK1-mediated autophagy Autophagy 2015-03-06 [PMID: 25749095] (WB, Human)
• Bercht Pfleghaar K, Taimen P et al. Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells. Nucleus 2015-05-03 [PMID: 25738644] (WB, Human)
• Park SJ, Jeong J, Park YU et al. Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics Sci Rep 2015-03-03 [PMID: 25732993] (WB, Human)
• Hsuan SW, Chyau CC, Hung HY et al. The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer Eur J Nutr 2015-02-27 [PMID: 25720497] (WB, Human)
• Xu N, Dong YJ, Yu QL et al. Convergent Regulation of Candida albicans Aft2 and Czf1 in Invasive and Opaque Filamentation J. Cell. Biochem. 2015-02-26 [PMID: 25716417] (WB, Fungi)
  
  Details:
  Alpha tubulin antibody used for WB in experiments involving several stains of Candida albicans.
• Yu Q, Jia C, Dong Y et al. Candida albicans autophagy, no longer a bystander: Its role in tolerance to ER stress-related antifungal drugs. Fungal Genet. Biol. 2015-02-21 [PMID: 25711686] (WB, Fungi)
  
  Details:
  Alpha-Tubulin antibody (DM1A) used for WB as a loading control on fungal cells that were untreated or treated with tunicamycin (Fig. 8A).
• Rucker H, Amslinger S. Identification of heme oxygenase-1 stimulators by a convenient ELISA-based bilirubin quantification assay. Free Radical Biology and Medicine. 2014-10-23 [PMID: 25462643] (WB, Mouse)
  
  Details:
  alpha Tubulin antibody used as a loading control for WB application (1:500 dilution, ECL detection, sharp band at 57kDa position) in experiments involving stimulation of murine macrophage RAW264.7 cells.
• Yu Q, Zhang B, Yang B et al. Interaction between the Vacuole, the Mitochondria and Oxidative Stress Response Is Governed by the Transient Receptor Potential Channel in Candida albicans. Free Radic. Biol. Med. 2014-10-09 [PMID: 25308698] (WB)
• Levine B. Autophagy is required for G1/G0 quiescence in response to nitrogen starvation in Saccharomyces cerevisiae. Autophagy. 2014-08-12 [PMID: 25126732] (ICC/IF, Yeast)
  
  Details:
  alpha Tubulin antibody used for ICC-IF on yeast strains - VPS30 delta/ATG6 delta, ATG7 delta, PEP4 delta subjected or not to starvation with nitrogen starvation medium/SD-N for 4 hours (Figure 4A).
• Doria M Luisa, Ribeiro Ana S, Wang Jun et al. Fatty acid and phospholipid biosynthetic pathways are regulated throughout mammary epithelial cell differentiation and correlate to breast cancer survival. FASEB J. 2014-06-26 [PMID: 24970396] (WB, Mouse)
• Stoeckelhuber M, Olzowy B, Ihler F et al. Immunolocalization of antimicrobial and cytoskeletal components in the serous glands of human sinonasal mucosa. Histol. Histopathol. 2014-04-15 [PMID: 24737387] (IHC-P, Human)
• Weng CM, Yu CC, Kuo ML, Lin CH. Endothelin-1 induces connective tissue growth factor expression in human lung fibroblasts by ETAR-dependent JNK/AP-1 pathway. Biochem. Pharmacol. 2014-02-21 [PMID: 24486572] (WB, Human)
• Hsieh YT, Chou MM, Chen HC, Tseng JJ. IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1. Gynecol Oncol. 2013-10-01 [PMID: 23911878]
• Barayuga SM, Pang X, Andres MA et al. Methamphetamine decreases levels of glutathione peroxidases 1 and 4 in SH-SY5Y neuronal cells: Protective effects of selenium. Neurotoxicology 2013-05-27 [PMID: 23721877] (WB, Human)
• Cheng X, Xu N, Yu Q et al. Novel insight into the expression and function of the multicopper oxidases in Candida albicans. Microbiology 2013-04-11 [PMID: 23579686] (WB, Fungi)
• Chandley MJ, Szebeni K, Szebeni A et al. Gene expression deficits in pontine locus coeruleus astrocytes in men with major depressive disorder. J Psychiatry Neurosci 2013-07-01 [PMID: 23415275] (WB, Human)
• Kim KW, Lee JM, Jeon YS et al. Vascular disrupting effect of CKD-516: preclinical study using DCE-MRI Invest New Drugs 2013-01-09 [PMID: 23299389] (IHC-Fr, ICC/IF, Human, Rabbit)
• Cavallo F, Graziani G, Antinozzi C et al. Reduced proficiency in homologous recombination underlies the high sensitivity of embryonal carcinoma testicular germ cell tumors to Cisplatin and poly (adp-ribose) polymerase inhibition PLoS One 2012-01-01 [PMID: 23251575] (WB, Human, Mouse)
• Gobeil PA, Leib DA. Herpes Simplex Virus gamma34.5 Interferes with Autophagosome Maturation and Antigen Presentation in Dendritic Cells MBio 2012-10-16 [PMID: 23073763] (WB, Mouse)
• Seale LA, Hashimoto AC, Kurokawa S et al. Disruption of the selenocysteine lyase-mediated selenium recycling pathway leads to metabolic syndrome in mice Mol Cell Biol 2012-10-01 [PMID: 22890841] (WB, Mouse)
• Gossett DR, Tse HT, Lee SA, Ying Y, Lindgren AG, Yang OO, Rao J, Clark AT, Di Carlo D. Hydrodynamic stretching of single cells for large population mechanical phenotyping. Proc Natl Acad Sci U S A. 2012-04-30 [PMID: 22547795] (ICC/IF, Human)
• Raman AV, Pitts MW, Seyedali A, Hashimoto AC, Seale LA, Bellinger FP, Berry MJ. Absence of selenoprotein P but not selenocysteine lyase results in severe neurological dysfunction. Genes Brain Behav. 2012-04-06 [PMID: 22487427] (WB, Mouse)
• Burmeister D, Aboushwareb T, D'Agostino R Jr et al. Impact of partial urethral obstruction on bladder function: time-dependent changes and functional correlates of altered expression of Ca2+ signaling regulators Am J Physiol Renal Physiol 2012-06-01 [PMID: 22442207] (WB, Rat)
• Yu Y H, Siao F P et al. TEX11 modulates germ cell proliferation by competing with estrogen receptor beta for the binding to HPIP. Mol Endocrinol 2012-01-04 [PMID: 22383461] (WB, Mouse)
• Ko WC, Chen BC, Hsu MJ et al. Thrombin induced connective tissue growth factor expression in rat vascular smooth muscle cells via the PAR-1/JNK/AP-1 pathway. Acta Pharmacol Sin. 2012-01-01 [PMID: 22212430] (WB, Rat)
• Hsu Y-F, Sheu J-R, Lin C-H et al. MAPK phosphatase-1 contributes to trichostatin A inhibition of cyclooxygenase-2 expression in human umbilical vascular endothelial cells exposed to lipopolysaccharide. Biochimica Et Biophysica Acta. 2011-09-01 [PMID: 21911040]
• Huang Z, Hoffmann FW, Norton RL et al. Selenoprotein K is a novel target of m-calpain and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages. J Biol Chem. 2011-08-17 [PMID: 21849499]
• Slupianek A, Dasgupta Y, Ren S-Y et al. Targeting RAD51 phosphotyrosine-315 to prevent unfaithful recombination repair in BCR-ABL1 leukemia. Blood;118(4):1062-8. 2011-07-28 [PMID: 21653319]
• Hoffmann FuKun W, Hashimoto Ann S, Lee Byung Cheon et al. Specific antioxidant selenoproteins are induced in the heart during hypertrophy. Archives of Biochemistry and Biophysics 2011-01-01 [PMID: 21621505] (WB, Mouse)
• Sander L E, Davis M J et al. Detection of prokaryotic mRNA signifies microbial viability and promotes immunity. Nature 2011-05-22 [PMID: 21602824] (WB, Mouse)
• Hsu YF, Sheu JR, Hsiao G et al. p53 in trichostatin A induced C6 glioma cell death. Biochim Biophys Acta 2011-05-01 [PMID: 21376104]
• Verma S, Hoffmann FW, Kumar M et al. Selenoprotein k knockout mice exhibit deficient calcium flux in immune cells and impaired immune responses. J Immunol;186(4):2127-2137. 2011-02-15 [PMID: 21220695]
• French RL, Heberlein U. Glycogen synthase kinase-3/Shaggy mediates ethanol-induced excitotoxic cell death of Drosophila olfactory neurons. Proc Natl Acad Sci U S A. 2009-11-18 [PMID: 19923438] (WB, Drosophila)
• Waldow T, Witt W, Janke A et al. Cell-cell junctions and vascular endothelial growth factor in rat lung as affected by ischemia/reperfusion and preconditioning with inhaled nitric oxide. J Surg Res 2009-11-01 [PMID: 19500802] (Rat)
• Panee J, Liu W et al. A novel function of bamboo extract in relieving lipotoxicity. Phytother Res 2008-01-05 [PMID: 18350521] (WB, Mouse)
• Odreman F et al. Proteomic studies on low- high-grade human brain astrocytomas. J Proteome Res 4:698-708 (2005). [PMID: 15952716] (WB, Human)
• Roychowdhury S et al. G protein binding G protein activation by nucleotide transfer involve distinct domains on tubulin: regulation of signal transduction by cytoskeletal elements. Biochemistry 32:4955-61. 1993-01-01 [PMID: 8490031]
• Blose SH et al. 10-nm filaments are induced to collapse in living cells microinjected with monoclonal polyclonal antibodies against tubulin. J Cell Biol 98:847-58. 1984-01-01 [PMID: 6538204]
• Berry S et al. The role of microtubules in platelet secretory release. Biochim Biophys Acta 1012:46-56. 1989-01-01 [PMID: 2730897] (IP, Human)
• Breitling F amp. Little M Carboxy-terminal regions on the surface of tubulin microtubules. Epitope locations of YOL1/34, DM1A DM1B. J Mol Biol 189:367-70. 1986-01-01 [PMID: 2427729]

Reviews for alpha Tubulin Antibody (NB100-690) (14)

Average Rating: 4.6

(Based on 14 reviews)

We have 14 reviews tested in 4 species: Human, Mouse, Human and Mouse, Other.

reviewed by: Jie Lan WB Human 05/18/2020

Summary

• Application: Western Blot
• Sample Tested: Breast cancer cells
• Species: Human
• Lot: A-4

reviewed by: Haiquan Lu WB Human 10/22/2018

Summary

• Application: Western Blot
• Sample Tested: Human breast cancer cell lines
• Species: Human
• Lot: A-4

reviewed by: Verified Customer WB Human and Mouse 06/29/2017

Summary

• Application: Western Blot
• Sample Tested: 293T cell lysate,nih3t3 cell lysate
• Species: Human and Mouse
• Lot: e-3

reviewed by: Verified Customer WB Other 07/15/2016

Summary

• Application: Western Blot
• Sample Tested: African Green Monkey Kidney (CV-1) whole cell lysate
• Species: Other
• Lot: E-1

reviewed by: Verified Customer WB Human 06/03/2014

Summary

• Application: Western Blot
• Species: Human

reviewed by: Verified Customer ICC Human 06/03/2014

Summary

• Application: Immunocytochemistry
• Species: Human

reviewed by: Verified Customer ICC Human 11/06/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: MDA-MB-231 cells
• Species: Human
• Lot: A2

reviewed by: Yu-Ting Yen WB Mouse 01/06/2012

Summary

• Application: Western Blot
• Sample Tested: mouse lung tissue
• Species: Mouse

reviewed by: Chien-Huang Lin WB Mouse 12/05/2011

Summary

• Application: Western Blot
• Sample Tested: Cell lysate
• Species: Mouse

reviewed by: Jing-Hsien Chen WB Human 11/23/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

reviewed by: Yu-Hsin Lin WB Human 11/23/2011

Summary

• Application: Western Blot
• Sample Tested: Human cell
• Species: Human

reviewed by: Shu-Wen Hsuan WB Human 11/21/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

reviewed by: Mou-Chieh Kao WB Human 11/18/2011

Summary

• Application: Western Blot
• Sample Tested: Human cell
• Species: Human

reviewed by: Wen- Wei Chang WB Human 10/31/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

FAQs for alpha Tubulin Antibody (NB100-690). (Showing 1 - 3 of 3 FAQs).

1. I am interested in buying an actin or tubulin antibody for Western blot loading control. We would like to use it on rat heart samples. Please recommend us one of your actin and/or tubulin products, and add it to the NOX2 quotation.
   • NB100-690 is for Alpha Tubulin and has been validated for use in WB and Rat. This will be added to the quote along with our Actin Antibody.
2. I am working on a dissection fixation protocol for a non model organism, and I am using DM1A alpha tubulin as a control.  I do not see any sort of Astral projections; does this convince you that it is a working stain ?
   • The staining looks a little off to my eye. What were your fixation conditions? Tubulin immunostaining can be finicky and requires particular fixation conditions for optimal labeling. Depending on your other antigenic sites, it's ideal to use either mixed aldehydes (PFA and Glutaraldehyde) with simultaneous extraction or methanol. PFA alone gives an undesirable hazy staining pattern.Some laboratories also recommend adding a calcium chelator into the fixation buffer as calcium induces depolymerization of microtubules. Lastly, be sure to run negative controls with your staining.
3. We would like to stain cilia with an acetylated alpha tubulin antibody in our cells, but I am unsure if this antibody will be able to conclusively differentiate cilia from other structures such as spindle pole bodies. Does anyone know what acetylated alpha tubulin antibodies might bind to apart from cilia?
   • Acetylated alpha tubulin is found in relatively stable microtubules. It is best practice to use this marker together with a centrosome/centriole marker, which will stain the basal bodies at the base of the cilium. After that, it is relatively straightforward to identify the acetylated alpha tubulin signal that corresponds to the cilium.

Bioinformatics

• Gene Symbol: TUBA1A
• Entrez:
  • 7846(Human)