alpha Tubulin Antibody (DM1A)

Images

 
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa and COS-7 lysates.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse prostate using alpha Tubulin antibody (clone DM1A) at 1:200 ...read more
Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody (DM1A) NB100-690AF488 (blue) and a matched isotype control (orange). Cells ...read more
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Western blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000).
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of alpha Tubulin expression in 1) HeLa, 2) NIH-3T3, 3) PC-12, 4) Cos-7, 5) CHO whole cell lysates and 6) pig brain tissue.
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analsis of alpha tubulin in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - IF analysis of alpha Tubulin in Human MDA-MB-231 cells. Verified customer review from 1DegreeBio.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - IF Confocal analysis of C6 cells using alpha Tubulin antibody (NB100-690, 1:50). An Alexa Fluor 488-conjugated Goat to mouse IgG was ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed colon sections. Heat mediated antigen retrieval was performed using sodium citrate buffer for 20 min before incubating with ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of colon tissue. Sections were formalin fixed and embedded with paraffin. Sodium citrate heat mediated antigen retrieval for 20 min. Incubated ...read more
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed paraffin embedded heart sections. Used at a dilution of 1:500.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of paraffin embedded colon sections.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of small intestine tissue fixed with formalin and paraffin embedded showing cytoplasmic and cytoskeletal staining of glandular cells.
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse skeletal muscle using alpha Tubulin antibody (clone DM1A) at 1:100 ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse lung using alpha Tubulin antibody (clone DM1A) at 1:100 dilution with ...read more
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse heart using alpha Tubulin antibody (clone DM1A) at 1:100 dilution ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using alpha Tubulin antibody (dark blue). Isotype control shown in orange. An ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on RAW 246.7 cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a ...read more
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on SH-SY5Y cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a ...read more
Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on U-937 cells with alpha Tubulin Antibody (DM1A) NB100-690AF647 (blue) and a matched isotype control (orange). ...read more
Simple Western: alpha Tubulin Antibody (DM1A) [NB100-690] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using ...read more
Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of skin fibroblasts.
Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of embryonic fibroblasts in the anaphase portion of mitosis.
Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of the marine parasite Cryptocaryon irritans mouth. Large bundles of microtubules form a cytophyrigeal basket.
Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa cells, green staining is alpha tubulin whereas red is DNA stained with propidium iodide.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, GP, Ha, Mk, Pa, Pm, Rb, Xp, YeSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready, ICC/IF
Clone
DM1A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

alpha Tubulin Antibody (DM1A) Summary

Description
Alpha tubulin antibody as a loading control: The TUBA1A gene is conserved evolutionarily and is ubiquitously expressed in most eukaryotic cell lines making it an attractive choice for a loading control target. Alpha tubulin detects around 50-55 kDa making it a good mid-range loading control antibody target. Quantitative western blotting requires a loading control so that differences in the loading of samples across wells can be accounted and adjusted for.
Immunogen
Native chicken brain microtubules.
Marker
Microtubule Marker
Specificity
Does not cross-react with beta Tubulin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Simple Western 1:50
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50000-1:100000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunomicroscopy
  • Immunoprecipitation 1:50-1:100
  • CyTOF-ready
  • Flow (Intracellular)
  • Immunofluorescence
Application Notes
This alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry. The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Tubulin alpha-1B Lysate (NBL1-17428)
Lung Lysate (NB820-59235)
Reviewed Applications
Read 12 Reviews rated 4.6
using
NB100-690 in the following applications:

Publications
Read Publications using
NB100-690 in the following applications:

Reactivity Notes

Human, mouse, rat, porcine, bovine, canine, chicken, guinea pig, Chinese hamster, monkey, avian, rabbit, Drosophila fungi and Xenopus. Yeast reactivity reported in scientific literature (PMID: 25126732).Will likely react with all mammals.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for alpha Tubulin Antibody (DM1A)

  • alpha tubulin loading control
  • alpha tubulin monoclonal
  • alpha Tubulin
  • Alpha-Tubulin 3
  • B-ALPHA-1
  • DM1A anti-tubulin
  • dm1a monoclonal
  • DM1a tubulin
  • DM1A
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA1 monoclonal
  • TUBA1A
  • TUBA3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-Alpha-1
  • tubulin monoclonal
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

The cytoskeleton consists of three major types of cytosolic fibers: microtubules (consisting of tubulin), microfilaments (actin filaments), and intermediate filaments. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6, b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins. This alpha tubulin loading control antibody was used in a study involving diet-induced obesity in selenocysteine lysase KO mice in order to normalize UCP1 expression levels by comparing to alpha tubulin levels(1). In another study looking at the effects of methamphetamine and selenium on GPx1 protein levels the alpha tubulin loading control antibody was used to account for any differences in the loading of GPx1 for western blot(2). The alpha tubulin loading control antibody was used in another study to account for differences in expression levels in cell lysates that were infected with different forms of the herpes simplex virus expressing different variants of the gamma 34.5 viral protein(3). PMIDs: 1. 26192035 Western Blot analysis using Alpha Tubulin Loading Control Antibody 2. 23721877 Alpha Tubulin Loading Control Antibody used in Western Blot 3. 23073763 Alpha Tubulin Loading Control Antibody Used to Normalize Expression Levels When studying cardiac tissue an alpha tubulin loading control antibody is recommended over beta tubulin because alpha tubulin is expressed in heart tissue whereas beta tubulin is not. Expression of alpha tubulin is very low or absent in stomach and oral mucosa tissue so it is not recommended to use an alpha tubulin loading control antibody when working with those tissues; however, a beta tubulin loading control antibody could be used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-690
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, GP, Ha, Mk, Pa, Pm, Rb, Xp, Ye
Applications: WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready, ICC/IF

Publications for alpha Tubulin Antibody (NB100-690)(64)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Drosophila, Fungi, Rabbit, Yeast.

We have publications tested in 7 applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


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(24)
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(15)
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Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using NB100-690 Applications Species
Kandel MB, Yamamoto S, Midorikawa R et al. N-glycosylation of the AMPA-type glutamate receptor regulates cell surface expression and tetramer formation affecting channel function J. Neurochem. Aug 9 2018 [PMID: 30092607] (WB, Human) WB Human
Nel M, Joubert AM, Dohle W et al. Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther. Jun 25 2018 [PMID: 29983544] (ICC/IF, Human) ICC/IF Human
Lin CY, Yang ST, Shen SC et al. Serum amyloid A1 in combination with integrin aVB3 increases glioblastoma cells mobility and progression Mol Oncol May 1 2018 [PMID: 29603594] (WB, Human) WB Human
Stock V, Sutter A, Raschke M, Queisser N. A tripartite mode of action approach for investigating the impact of aneugens on tubulin polymerization. Environ. Mol. Mutagen. 2017 Dec 04 [PMID: 29205516] (Flow)

Details:
This citation used the PE form of this antbody
Flow
Rodrigues BA, Kuga GK, Munoz VR et al. Overexpression of Mitogen-activated protein kinase phosphatase-3 (MKP-3) reduces FoxO1 phosphorylation in mice hypothalamus Neurosci. Lett. 2017 Sep 01 [PMID: 28866049] (Mouse) Mouse
Chen PC, Hsieh MH, Wen-ShuoKuo P et al. Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis. J. Allergy Clin. Immunol. Apr 12 2017 [PMID: 28412394] (WB) WB
Sidoli M, Musner N, Silvestri N et al. Ablation of Perk in Schwann Cells Improves Myelination in the S63del Charcot-Marie-Tooth 1B Mouse. J. Neurosci. Nov 2 2016 [PMID: 27807175] (WB, Mouse) WB Mouse
Rueli RH, Torres DJ, Dewing AS et al. Selenoprotein S Reduces Endoplasmic Reticulum Stress-Induced Phosphorylation of Tau: Potential Role in Selenate Mitigation of Tau Pathology. J. Alzheimers Dis. Oct 11 2016 [PMID: 27802219]
Nakamura M, Nishikawa J, Saito M et al. Decitabine inhibits tumor cell proliferation and up-regulates E-cadherin expression in Epstein-Barr virus-associated gastric cancer J. Med. Virol. 2016 Jul 19 [PMID: 27430892] (IHC, Human) IHC Human
Chang CF, Hsu LS, Weng CY et al. N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability. Int J Mol Sci Jun 18 2016 [PMID: 27314333] (WB,) WB
Show All 64 Publications.

Reviews for alpha Tubulin Antibody (NB100-690) (12) 4.612

Average Rating: 4.6
(Based on 12 reviews)
We have 12 reviews tested in 4 species: Human, Mouse, Human and Mouse, Other.

Reviews using NB100-690:
Filter by Applications
WB
(10)
ICC
(2)
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Human
(8)
Mouse
(2)
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(1)
Other
(1)
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Images Ratings Applications Species Date Details
Western Blot alpha Tubulin NB100-690
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reviewed by:
Jun Hamazaki
WB Human and Mouse 06/29/2017
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Summary

ApplicationWestern Blot
Sample Tested293T cell lysate,nih3t3 cell lysate
SpeciesHuman and Mouse
Lote-3
Western Blot alpha Tubulin NB100-690
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reviewed by:
Emily Ledgerwood
WB Other 07/15/2016
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Summary

ApplicationWestern Blot
Sample TestedAfrican Green Monkey Kidney (CV-1) whole cell lysate
SpeciesOther
LotE-1

Blocking

Blocking Details1 hour in 5% milk/TBST

Primary Anitbody

Dilution Ratioalpha tubulin NB100-690 1:5000 in 5% milk/TBST overnight at 4 degrees. This is a membrane that was stripped and reprobed.

Secondary Antibody

Secondary DescriptionDonkey anti-mouse HRP
Secondary Manufacturer Cat#715-035-150
Secondary Concentration1:10000

Details

Detection NotesExposed on a LiCor C-Digit using Advansta WesternBright Quantum

Comments

CommentsThis is a fantastic antibody for alpha-tubulin that I have been using for years. For this review, I opted to submit an image of a PVDF membrane that was originally probed for a different primary, stripped using LiCor's Western Blot Stripping Buffer according to manufacturer's instructions and then reprobed with this alpha-tubulin antibody. To top it off, I am imaging on a C-digit which is the cheapest imager on the market. This antibody is extremely reliable and well worth the money.
Western Blot alpha Tubulin NB100-690
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reviewed by:
Anony- mous
WB Human 06/03/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000).
FileView PDF
Immunocytochemistry alpha Tubulin NB100-690
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reviewed by:
Anony- mous
ICC Human 06/03/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of C6 cells using alpha Tubulin antibody (NB100-690, 1:50). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF
Immunocytochemistry alpha Tubulin NB100-690
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reviewed by:
Samantha Garcia
ICC Human 11/06/2013
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Summary

ApplicationImmunocytochemistry
Sample TestedMDA-MB-231 cells
SpeciesHuman
LotA2

Blocking

Blocking DetailsPBS with 2% BSA

Primary Anitbody

Dilution Ratio1:2000 in blocking buffer

Details

Fixation DetailsMDA-MB-231 cells were fixed in 4% PFA. Glutaraldehyde fixation also worked.
Western Blot alpha Tubulin NB100-690
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reviewed by:
Anonymous
WB Mouse 01/06/2012
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Summary

ApplicationWestern Blot
Sample Testedmouse lung tissue
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 100ug FBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL, Exposure Time: 1 minute, Specific Bands: 51 kDa, Non-Specific Bands: 50-55 kDa
 
reviewed by:
Anonymous
WB Mouse 12/05/2011
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesMouse
CommentsThe signal is strong and clear.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 2 hours

Secondary Antibody

Secondary DescriptionSecondary Ab: mouse HRP

Details

Detection NotesDetection Method: ECL with X-ray film

Comments

CommentsThe signal is strong and clear.
 
reviewed by:
Anonymous
WB Human 11/23/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsWorked well

Blocking

Blocking DetailsBlocking Time: 1 hour

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: mouse

Details

Detection NotesDetection Method: HRP

Comments

CommentsWorked well
 
reviewed by:
Anonymous
WB Human 11/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman cell
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse

Details

Detection NotesDetection Method: HRP
 
reviewed by:
Anonymous
WB Human 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk in TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit

Details

Detection NotesDetection Method: Western Lighting plus ECL
 
reviewed by:
Anonymous
WB Human 11/18/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman cell
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:5000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP

Details

Detection NotesDetection Method: chemiluminescence system
 
reviewed by:
Anonymous
WB Human 10/31/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGreat performance! Clear band in signal

Blocking

Blocking DetailsBlocking Buffer: 5% Skim Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP conjugated anti-mouse

Details

Detection NotesDetection Method: Chemiluminescence

Comments

CommentsGreat performance! Clear band in signal

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin Antibody (NB100-690). (Showing 1 - 7 of 7 FAQs).

  1. We are working with mouse liver tissue, will this alpha tubulin antibody work as a loading control?
    • Yes, alpha tubulin is expressed in nearly all tissue types including liver; however we do not recommend using as a loading control antibody in stomach or oral mucosa tissue as expression of alpha tubulin in these areas is minimal.
  2. Will your alpha tubulin antibody work as a loading control for simple western?
    • Yes, this alpha tubulin antibody has been validated in simple western and makes an excelent loading control antibody. Simple western has a lower detection limit of 12kDA and alpha tubulin detects around 55kDA.
  3. Will this alpha tubulin antibody recognize both isoforms of alpha tubulin?
    • The epitope for this alpha tubulin antiboyd lies on the C-terminus of the protein and the difference between the two major isoforms is witin the first 35 aa of the N-terminus so this alpha tubulin antibody will recognize both isoforms.
  4. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.
  5. We're looking at LC3B, aSYN and p62 which are 18, 23 and 65 kDA respectively so we need the loading control antibody to be between those.
    • Alpha tubulin antibody detects around 55kDA so that would be a good option for a loading control antibody for those targets as you should be able to easily distinguish from the other bands.
  6. I am looking for a primary alpha tubulin  antibody that is conjugated to a red fluorophore.
    • Okay, our most popular alpha tubulin antibody is NB100-690 and is available in a variety of formats. So if you are looking for red I would recommend a DyLight 550, 650 etc.
  7. I am interested in buying an actin or tubulin antibody for Western blot loading control. We would like to use it on rat heart samples. Please recommend us one of your actin and/or tubulin products, and add it to the NOX2 quotation.
    • NB100-690 is for Alpha Tubulin and has been validated for use in WB and Rat. This will be added to the quote along with our Actin Antibody.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-690AF405
Alexa Fluor 488 Labeled NB100-690AF488
Alexa Fluor 647 Labeled NB100-690AF647
Alexa Fluor 700 Labeled NB100-690AF700
Allophycocyanin Labeled NB100-690APC
Biotin Labeled NB100-690B
DyLight 350 Labeled NB100-690UV
DyLight 405 Labeled NB100-690V
DyLight 488 Labeled NB100-690G
DyLight 550 Labeled NB100-690R
DyLight 650 Labeled NB100-690C
DyLight 680 Labeled NB100-690FR
DyLight 755 Labeled NB100-690IR
FITC Labeled NB100-690F
HRP Labeled NB100-690H
PE Labeled NB100-690PE
PerCP Labeled NB100-690PCP

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Blogs on alpha Tubulin.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Alpha Tubulin: A Fundamental Cytoskeleton Protein with Many Roles
The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Tubulins are the microtubule building block and exist as globular dimeric proteins of alpha/beta chains....  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

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