SAE2 Antibody (OTI4A3)

Images

 
Western Blot: SAE2 Antibody (4A3) [NBP2-03688] Analysis of extracts (35ug) from 9 different cell lines by using anti-SAE2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SAE2 Antibody (4A3) [NBP2-03688] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAE2.
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Human prostate tissue using anti-SAE2 mouse monoclonal antibody.
Western Blot: SAE2 Antibody (4A3) [NBP2-03688] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SAE2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Human endometrium tissue using anti-SAE2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Human lymphoma tissue using anti-SAE2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.41 mg/ml

SAE2 Antibody (OTI4A3) Summary

Immunogen
Full length human recombinant protein of human UBA2 (NP_005490) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
UBA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.41 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAE2 Antibody (OTI4A3)

  • Anthracycline-associated resistance ARX
  • ARX
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ13058
  • HRIHFB2115
  • SAE2SUMO-1 activating enzyme subunit 2
  • SUMO1 activating enzyme subunit 2
  • SUMO-activating enzyme subunit 2
  • UBA2, ubiquitin-activating enzyme E1 homolog
  • Ubiquitin-like 1-activating enzyme E1B
  • ubiquitin-like modifier activating enzyme 2
  • UBLE1B

Background

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SAE2 Antibody (NBP2-03688) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73973AF350
Alexa Fluor 405 NBP2-73973AF405
Alexa Fluor 488 NBP2-73973AF488
Alexa Fluor 532 NBP2-73973AF532
Alexa Fluor 594 NBP2-73973AF594
Alexa Fluor 647 NBP2-73973AF647
Alexa Fluor 700 NBP2-73973AF700
Alexa Fluor 750 NBP2-73973AF750
Biotin NBP2-73973B
DyLight 350 NBP2-73973UV
DyLight 405 NBP2-73973V
DyLight 488 NBP2-73973G
DyLight 550 NBP2-73973R
DyLight 594 NBP2-73973DL594
DyLight 650 NBP2-73973C
DyLight 680 NBP2-73973FR
DyLight 755 NBP2-73973IR
FITC NBP2-73973F
HRP NBP2-73973H
Janelia Fluor 549 NBP2-73973JF549
Janelia Fluor 646 NBP2-73973JF646

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Bioinformatics

Gene Symbol UBA2
Entrez