SAE2 Antibody (OTI4A3)


Western Blot: SAE2 Antibody (4A3) [NBP2-03688] Analysis of extracts (35ug) from 9 different cell lines by using anti-SAE2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SAE2 Antibody (4A3) [NBP2-03688] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAE2.
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Human prostate tissue using anti-SAE2 mouse monoclonal antibody.
Western Blot: SAE2 Antibody (4A3) [NBP2-03688] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 more
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SAE2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Human endometrium tissue using anti-SAE2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Human lymphoma tissue using anti-SAE2 mouse monoclonal antibody.

Product Details

Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC
1 mg/ml

SAE2 Antibody (OTI4A3) Summary

Full length human recombinant protein of human UBA2 (NP_005490) produced in HEK293T cell.
Immunogen affinity purified
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  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for SAE2 Antibody (OTI4A3)

  • Anthracycline-associated resistance ARX
  • ARX
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ13058
  • HRIHFB2115
  • SAE2SUMO-1 activating enzyme subunit 2
  • SUMO1 activating enzyme subunit 2
  • SUMO-activating enzyme subunit 2
  • UBA2, ubiquitin-activating enzyme E1 homolog
  • Ubiquitin-like 1-activating enzyme E1B
  • ubiquitin-like modifier activating enzyme 2
  • UBLE1B


Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Gene Symbol UBA2