alpha Tubulin Antibody (DM1A) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, GP, Ha, Mk, Pa, Pm, Rb, Xp, YeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
DM1A
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

alpha Tubulin Antibody (DM1A) [DyLight 488] Summary

Immunogen
Native chicken brain microtubules.
Marker
Microtubule Marker
Specificity
Does not cross-react with beta Tubulin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This alpha Tubulin Antibody (DM1A) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry. NB100-690 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, porcine, bovine, canine, chicken, guinea pig, Chinese hamster, monkey, avian, rabbit, Drosophila fungi and Xenopus. Yeast reactivity reported in scientific literature (PMID: 25126732).Will likely react with all mammals.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for alpha Tubulin Antibody (DM1A) [DyLight 488]

  • alpha Tubulin
  • Alpha-Tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA1A
  • TUBA3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-Alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-690G
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, GP, Ha, Mk, Pa, Pm, Rb, Xp, Ye
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin Antibody (NB100-690G). (Showing 1 - 6 of 6 FAQ).

  1. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.
  2. We are working with mouse liver tissue, will this alpha tubulin antibody work as a loading control?
    • Yes, alpha tubulin is expressed in nearly all tissue types including liver; however we do not recommend using as a loading control antibody in stomach or oral mucosa tissue as expression of alpha tubulin in these areas is minimal.
  3. Will your alpha tubulin antibody work as a loading control for simple western?
    • Yes, this alpha tubulin antibody has been validated in simple western and makes an excelent loading control antibody. Simple western has a lower detection limit of 12kDA and alpha tubulin detects around 55kDA.
  4. Will this alpha tubulin antibody recognize both isoforms of alpha tubulin?
    • The epitope for this alpha tubulin antiboyd lies on the C-terminus of the protein and the difference between the two major isoforms is witin the first 35 aa of the N-terminus so this alpha tubulin antibody will recognize both isoforms.
  5. We're looking at LC3B, aSYN and p62 which are 18, 23 and 65 kDA respectively so we need the loading control antibody to be between those.
    • Alpha tubulin antibody detects around 55kDA so that would be a good option for a loading control antibody for those targets as you should be able to easily distinguish from the other bands.
  6. I am looking for a primary alpha tubulin  antibody that is conjugated to a red fluorophore.
    • Okay, our most popular alpha tubulin antibody is NB100-690 and is available in a variety of formats. So if you are looking for red I would recommend a DyLight 550, 650 etc.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-690APC
Biotin Labeled NB100-690B
DyLight 350 Labeled NB100-690UV
DyLight 405 Labeled NB100-690V
DyLight 550 Labeled NB100-690R
DyLight 650 Labeled NB100-690C
DyLight 680 Labeled NB100-690FR
DyLight 755 Labeled NB100-690IR
FITC Labeled NB100-690F
HRP Labeled NB100-690H
PE Labeled NB100-690PE
PerCP Labeled NB100-690PCP

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Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Alpha Tubulin: A Fundamental Cytoskeleton Protein with Many Roles
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Bioinformatics

Gene Symbol TUBA1A
Entrez