Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit Summary

• Background: The Quantikine Human PLA2G7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure PLA2G7 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human PLA2G7 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PLA2G7 showed linear curves that were parallel to the curves obtained using the... Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PLA2G7.
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• Specificity: Natural and recombinant human PLA2G7
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: PLA2G7

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit

• 2-acetyl-1-alkylglycerophosphocholine esterase
• EC 3.1.1
• EC 3.1.1.47,1-alkyl-2-acetylglycerophosphocholine esterase
• Group-VIIA phospholipase A2
• gVIIA-PLA2
• LDL-associated phospholipase A2
• LDL-PLA(2)
• LDL-PLA2
• lipoprotein-associated phospholipase A2
• LpPLA2
• Lp-PLA2
• PAF acetylhydrolase
• PAF-AH
• PAFAHPAF 2-acylhydrolase
• phospholipase A2, group VII (platelet-activating factor acetylhydrolase
• PLA2G7
• plasma)
• platelet-activating factor acetylhydrolase

Background

Secretory phopholipase A2 (PLA2) enzymes cleave an acyl ester bond in the sn-2 position of glycerophospholipids. These extracellular proteins have a high disulfide bond content, low molecular mass (14 kDa), and require mM levels of Ca2+ for catalysis. They play a crucial role in the generation of arachidonates and eicosanoids, and have a number of biological actions including immunological responses, inflammation, cellular proliferation, vasoconstriction, and bronchioconstriction.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for PLA2G7/PAF-AH/Lp-PLA2 (DPLG70)(14)

Publications using DPLG70

• M Yang, Z Yang, Y Li, S Su, Z Li, D Lu Mechanism of Traditional Tibetan Medicine Grubthobrildkr Alleviated Gastric Ulcer Induced by Acute Systemic Hypoxia in Rats BioMed Research International, 2022-04-05;2022(0):4803956. 2022-04-05 [PMID: 35425842] (Rat)
• Y Liao, S Badmann, T Kaltofen, D Mayr, E Schmoeckel, E Deuster, M Mannewitz, S Landgrebe, T Kolben, A Hester, S Beyer, A Burges, S Mahner, U Jeschke, F Trillsch, B Czogalla Platelet-Activating Factor Acetylhydrolase Expression in BRCA1 Mutant Ovarian Cancer as a Protective Factor and Potential Negative Regulator of the Wnt Signaling Pathway Biomedicines, 2021-06-22;9(7):. 2021-06-22 [PMID: 34206491] (Human)
• U Hoffmann, MT Lu, D Olalere, EC Adami, MT Osborne, A Ivanov, JS Aluru, S Lee, N Arifovic, ET Overton, CJ Fichtenbau, JA Aberg, B Alston-Smi, KL Klingman, M Waclawiw, TH Burdo, KC Williams, MV Zanni, P Desvigne-N, K Cooper-Arn, KV Fitch, H Ribaudo, PS Douglas, SK Grinspoon Rationale and design of the Mechanistic Substudy of the Randomized Trial to Prevent Vascular Events in HIV (REPRIEVE): Effects of pitavastatin on coronary artery disease and inflammatory biomarkers Am. Heart J., 2019-03-04;212(0):1-12. 2019-03-04 [PMID: 30928823] (Human)
• X Zhao, J Li, Y Meng, M Cao, J Wang Treatment Effects of Jinlingzi Powder and Its Extractive Components on Gastric Ulcer Induced by Acetic Acid in Rats Evid Based Complement Alternat Med, 2019-01-03;2019(0):7365841. 2019-01-03 [PMID: 30719066] (Rat)
• Y Shen, L Chen, W Yang, Z Wang Exploration of serum sensitive biomarkers of fatty liver in dairy cows Sci Rep, 2018-09-11;8(1):13574. 2018-09-11 [PMID: 30206404] (Bovine)
• H Liu, Y Yao, Y Wang, L Ji, K Zhu, H Hu, J Chen, J Yang, Q Cui, B Geng, Q Liu, D Li, Y Zhou Association between high-sensitivity C-reactive protein, lipoprotein-associated phospholipase A2 and carotid atherosclerosis: A cross-sectional study J. Cell. Mol. Med., 2018-08-09;0(0):. 2018-08-09 [PMID: 30094934] (Human)
• CY Ma, ZY Xu, SP Wang, HY Peng, F Liu, JH Liu, FX Ren Change of Inflammatory Factors in Patients with Acute Coronary Syndrome Chin. Med. J., 2018-06-20;131(12):1444-1449. 2018-06-20 [PMID: 29893361] (Human)
• E Kimak, B Zi?ba, D Duma, J Solski Myeloperoxidase level and inflammatory markers and lipid and lipoprotein parameters in stable coronary artery disease Lipids Health Dis, 2018-04-04;17(1):71. 2018-04-04 [PMID: 29618370] (Human)
• L Jackisch, W Kumsaiyai, JD Moore, N Al-Daghri, I Kyrou, TM Barber, H Randeva, S Kumar, G Tripathi, PG McTernan Differential expression of Lp-PLA2 in obesity and type 2 diabetes and the influence of lipids Diabetologia, 2018-02-09;0(0):. 2018-02-09 [PMID: 29427237] (ELISA Capture, Human)
• Y Han, EY Kwon, MK Yu, SJ Lee, HJ Kim, SB Kim, YH Kim, MS Choi A Preliminary Study for Evaluating the Dose-Dependent Effect of d-Allulose for Fat Mass Reduction in Adult Humans: A Randomized, Double-Blind, Placebo-Controlled Trial Nutrients, 2018-01-31;10(2):. 2018-01-31 [PMID: 29385054] (ELISA Capture, Human)
• C Topbas, A Swick, M Razavi, NL Anderson, TW Pearson, C Bystrom Measurement of Lipoprotein-Associated Phospholipase A2 by Use of 3 Different Methods: Exploration of Discordance between ELISA and Activity Assays Clin. Chem., 2018-01-10;0(0):. 2018-01-10 [PMID: 29321128] (Human)
• A transcriptome-based global map of signaling pathways in the ovarian cancer microenvironment associated with clinical outcome Genome Biol, 2016-05-23;17(1):108. 2016-05-23 [PMID: 27215396] (Human)
• Takata K, Imaizumi S, Kawachi E, Suematsu Y, Shimizu T, Abe S, Matsuo Y, Tsukahara H, Noda K, Yahiro E, Zhang B, Uehara Y, Miura S, Saku K Impact of cigarette smoking cessation on high-density lipoprotein functionality. Circ J, 2014-10-16;78(12):2955-62. 2014-10-16 [PMID: 25319317] (Human)
• Lopez-Farre AJ, Zamorano-Leon JJ, Segura A, Mateos-Caceres PJ, Modrego J, Rodriguez-Sierra P, Calatrava L, Tamargo J, Macaya C Plasma desmoplakin I biomarker of vascular recurrence after ischemic stroke. J. Neurochem., 2012-03-13;121(2):314-25. 2012-03-13 [PMID: 22304020] (Human)

FAQs for PLA2G7/PAF-AH/Lp-PLA2 (DPLG70). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PLA2G7