beta Tubulin Antibody

Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification].

Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification].

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification].

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification].

Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, In, Pm, Xp, Ze
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, Single Cell Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

beta Tubulin Antibody Summary

• Immunogen: A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot: P07437]
• Localization: Major constituent of microtubules
• Marker: Microtubule Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:1000 - 1:2000
  • Immunohistochemistry-Paraffin 1:1000 - 1:2000
  • Single Cell Western 1:40
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  beta Tubulin Overexpression Lysate

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 28114363). C. elegans reactivity reported in scientific literature (PMID: 27690361).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta Tubulin Antibody

• beta polypeptide
• MGC117247
• Tubb5
• tubulin beta chain
• tubulin beta polypeptide
• tubulin beta-1 chain
• Tubulin beta-5 chain
• tubulin, beta

Background

The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments, and tubulins comprises the microtubule building block. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6, b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Tubulin Antibody (NB600-936)(34)

Publications using NB600-936

• Gyongyosi M, Lukovic D, Zlabinger K et al. Liposomal doxorubicin attenuates cardiotoxicity via induction of interferon-related DNA damage resistance Cardiovasc. Res. Jul 25 2019 [PMID: 31346605] (WB, Porcine)
• Hannen R, Selmansberger M, Hauswald M et al. Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor Cancers (Basel) Jun 30 2019 [PMID: 31262047] (WB, Human)
• Scharfenberg F, Helbig A, Sammel M et al. Degradome of soluble ADAM10 and ADAM17 metalloproteases Cell. Mol. Life Sci. Jun 17 2019 [PMID: 31209506]
• Baraliakos X, Boehm H, Bahrami R et al. Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload Neuroscience May 23 2019 [PMID: 31129202] (ICC/IF, Rat)
• Lee BJ, Jun HO, Kim JH, Kim JH. Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina FASEB J. Feb 11 2019 [PMID: 30742774] (WB, Human)
• Castle AR, Daude N, Gilch S, Westaway D. Application of high-throughput, capillary-based Western analysis to modulated cleavage of the cellular prion protein J. Biol. Chem. Dec 21 2018 [PMID: 30578300] (WB, Mouse)
• Hannen R, Steffani M, Voellger B et al. Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study. J. Steroid Biochem. Mol. Biol. Nov 13 2018 [PMID: 30439415] (WB, Rat)
• Stuart JA, Fonseca J, Moradi F et al. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxid Med Cell Longev. Sep 30 2018 [PMID: 30363917] (WB, Human, Mouse)
• Yang L, Chen L, Lu X et al. Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice Reproduction May 29 2018 [PMID: 29844226] (WB, Mouse)
• Mao Z, Yang L, Lu X, Tan A. C1QTNF3 in the murine ovary and its function in folliculogenesis. Reproduction. 2018 Feb 01 [PMID: 29438034] (WB, Mouse)
• Schulte ML, Fu A, Zhao P et al. Pharmacological blockade of ASCT2-dependent glutamine transport leads to antitumor efficacy in preclinical models. Nat. Med. Feb 1 2018 [PMID: 29334372] (WB, Human)
• Bengoetxea H, Rico-Barrio I, Ortuzar N et al. Environmental Enrichment Reverses Tyrosine Kinase Inhibitor-Mediated Impairment Through BDNF-TrkB Pathway Mol. Neurobiol. 2017 Aug 25 [PMID: 28842826] (Rat)
• Requejo C, Ruiz-Ortega JA, Bengoetxea H et al. Deleterious Effects of VEGFR2 and RET Inhibition in a Preclinical Model of Parkinson's Disease Mol. Neurobiol. 2017 Aug 24 [PMID: 28840516] (Rat)
• Requejo C, Ruiz-Ortega JA, Cepeda H et al. Nanodelivery of Cerebrolysin and Rearing in Enriched Environment Induce Neuroprotective Effects in a Preclinical Rat Model of Parkinson's Disease Mol. Neurobiol. 2017 Aug 24 [PMID: 28840482] (Rat)
• Gelmedin V, Morel M, Hahnel S et al. Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival. PLoS Pathog [PMID: 28114363] (ICC/IF, Invertebrate)
• Lau AC, Zhu KP, Brouhard EA et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin. Oct 25 2016 [PMID: 27777629] (WB)
• Snyder MJ, Lau AC, Brouhard EA et al. Anchoring of Heterochromatin to the Nuclear Lamina Reinforces Dosage Compensation-Mediated Gene Repression. PLoS Genet. Sep 1 2016 [PMID: 27690361]
• Lee MC, Huang HJ, Chang TH et al. Genome-wide analysis of HIF-2alpha chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions. Sci Rep 2016 Jul 04 [PMID: 27373565] (WB)
• Ali AA, Lee YR, Chen TC et al. Novel Anthra[1,2-c][1,2,5]Thiadiazole-6,11-Diones as Promising Anticancer Lead Compounds: Biological Evaluation, Characterization & Molecular Targets Determination. PLoS One 2016 Apr 21 [PMID: 27100886] (WB)
• McKinley ET, Zhao P, Coffey RJ et al. 3'-Deoxy-3'-[18F]-Fluorothymidine PET Imaging Reflects PI3K-mTOR-Mediated Pro-Survival Response to Targeted Therapy in Colorectal Cancer. PLoS ONE. 2014 Sep 24 [PMID: 25247710] (WB, Mouse)
• Chen WH, Cheng SJ, Tzen JT et al. Probing Relevant Molecules in Modulating the Neurite Outgrowth of Hippocampal Neurons on Substrates of Different Stiffness. PLoS One 2013 Dec 30 [PMID: 24386192] (ICC/IF, Mouse)
• Miklosz A, Lukaszuk B, Baranowski M et al. Effects of Inhibition of Serine Palmitoyltransferase (SPT) and Sphingosine Kinase 1 (SphK1) on Palmitate Induced Insulin Resistance in L6 Myotubes. PLoS One 2013 Dec 23 [PMID: 24376889] (WB, Rat)
• Lu ZH, Kaliberov S, Sohn R et al. Transcriptional Targeting of Primary and Metastatic Tumor Neovasculature by an Adenoviral Type 5 Roundabout4 Vector in Mice. PLoS One 2013 Dec 23 [PMID: 24376772] (WB, Mouse)
• Orlicky DJ, Monks J, Stefanski AL, McManaman JL. Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion. PLoS One 2013 Jun 25 [PMID: 23825572]
• Riva M, He Z, Kallberg E et al. Human S100A9 Protein Is Stabilized by Inflammatory Stimuli via the Formation of Proteolytically-Resistant Homodimers. PLoS One 2013 Apr 23 [PMID: 23626736] (WB, Human, Primate)
• McKinley ET, Smith RA, Zhao P et al. 3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer. Chem Biol Interact. 2013 Mar 25 [PMID: 23341544] (WB, Human)
• Jacek E, Fallon BA, Chandra A et al. Increased IFNalpha activity and differential antibody response in patients with a history of Lyme disease and persistent cognitive deficits J Neuroimmunol 2012 Nov 7 [PMID: 23141748] (WB, Mouse)
• Li Z, Zhao ZJ, Zhu XQ, Ren QS, Nie FF, Gao JM, Gao XJ, Yang TB, Zhou WL, Shen JL, Wang Y, Lu FL, Chen XG, Hide G, Ayala FJ, Lun ZR. Differences in iNOS and Arginase Expression and Activity in the Macrophages of Rats Are Responsible for the Resistance against T gondii Infection. PLoS One;7(4). 2012 [PMID: 22558235] (WB, Mouse, Rat)
• Kaucher AV, Oatley MJ, Oatley JM. NEUROG3 is a critical downstream effector for STAT3-regulated differentiation of mammalian stem and progenitor spermatogonia Biol Reprod 2012 May 31 [PMID: 22378757] (WB, Mouse)
• Kadiu I, Gendelman HE. Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection J Neuroimmune Pharmacol 2011 Dec [PMID: 21789505] (ICC/IF, Human)
• Lin SY, Chen NT, Sun SP et al. The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death. J Am Chem Soc. 2010 Jun 23 [PMID: 20499915] (WB, Human)
• Reding SC, Stepnoski AL, Cloninger EW, Oatley JM. THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis. Reproduction;139(5):893-903. 2010 May. [PMID: 20154176] (WB, Bovine)
• Catalucci D, Zhang D-H, DeSantiago J et al. Akt regulates L-type Ca2+ channel activity by modulating Cav{alpha}1 protein stability. J Cell Biol;184(6):923-933. 2009 [PMID: 19307602]
• Rattner A, Toulabi L, Williams J et al. The genomic response of the retinal pigment epithelium to light damage and retinal detachment. J Neurosci. 2008 Sep 2428(39):9880-9. [PMID: 18815272]

Reviews for beta Tubulin Antibody (NB600-936) (6)

Average Rating: 4.5

(Based on 6 reviews)

We have 6 reviews tested in 3 species: Human, Mouse, Other.

reviewed by: Anonymous Simple Western Mouse 01/21/2016

Summary

• Application: Simple Western
• Sample Tested: whole brain homogenates from mice
• Species: Mouse

Blocking

• Blocking Details: n/a

Primary Anitbody

• Dilution Ratio: 1:10,000, 1:5,000, 1:1,000

Details

• Detection Notes: Wes Simple Western platform

Comments

• Comments: Best at lower concentrations due to some faint high molecular weight bands

reviewed by: Liyong Zhang WB Mouse 07/01/2014

Summary

• Application: Western Blot
• Species: Mouse
• File: View PDF

reviewed by: Anony- mous ICC Mouse 06/12/2014

Summary

• Application: Immunocytochemistry
• Species: Mouse
• Special Applications: Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
• File: View PDF

reviewed by: Anony- mous WB Human 05/27/2014

Summary

• Application: Western Blot
• Species: Human
• File: View PDF

reviewed by: Anonymous IHC Mouse 01/13/2012

Summary

• Application: Immunohistochemistry
• Sample Tested: Hippocampal Primary Culture
• Species: Mouse

Blocking

• Blocking Details: Blocking Buffer: 5% BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

• Secondary Description: Secondary Ab: Jackson / Goat anti Rabbit IgG DyLight488, Secondary Ab Dilution Ratio: 1:5000

Details

• Detection Notes: Detection Visualization Method: Microscope

reviewed by: Anonymous WB Other 11/09/2011

Summary

• Application: Western Blot
• Sample Tested: Chicken cell line
• Species: Other
• Comments: Good!

Blocking

• Blocking Details: Blocking Buffer: BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: anti-mouse HRT

Details

• Detection Notes: Detection Method: ECL

Comments

• Comments: Good!

FAQs for beta Tubulin Antibody (NB600-936). (Showing 1 - 2 of 2 FAQs).

1. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
   • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.
2. We are looking for an antibody which is a specific marker for Mouse Bronchial cilia in IHC(p) technique. Preferably not from a mouse host. Can you help?
   • According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459

Bioinformatics

• Gene Symbol: TUBB
• Entrez:
  • 203068(Human)
  • 545486(Mouse)
  • 498736(Rat)
• Uniprot:
  • P07437(Human)