beta Tubulin Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000
Biological Strategies: Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) ...read more
Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.
Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

beta Tubulin Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot: P07437]
Localization
Major constituent of microtubules
Marker
Microtubule Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:1000 - 1:2000
  • Immunohistochemistry-Paraffin 1:1000 - 1:2000
  • Simple Western 1:1000
  • Western Blot 1:1000
Application Notes
Use in Simple Western reported in scientific literature (PMID:35008713) In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. SCW validated using murine pancreatic cancer cells.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
beta Tubulin Overexpression Lysate
Reviewed Applications
Read 7 Reviews rated 4.4
using
NB600-936 in the following applications:

Publications
Read Publications using
NB600-936 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34519641). Invertebrate reactivity reported in scientific literature (PMID: 28114363). C. elegans reactivity reported in scientific literature (PMID: 27690361). Insect reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta Tubulin Antibody - BSA Free

  • beta polypeptide
  • beta Tubulin
  • MGC117247
  • OK/SW-cl.56
  • TUBB
  • TUBB5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Background

The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments, and tubulins comprises the microtubule building block. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6 , b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
NB600-936
Species: Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for beta Tubulin Antibody (NB600-936)(68)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Bovine, C. elegans, Invertebrate, Porcine, Primate, Xenopus.

We have publications tested in 5 applications: Flow, ICC/IF, SW, Simple Western, WB.


Filter By Application
Flow
(1)
ICC/IF
(4)
SW
(1)
Simple Western
(1)
WB
(44)
All Applications
Filter By Species
Human
(21)
Mouse
(14)
Rat
(10)
Bovine
(1)
C. elegans
(1)
Invertebrate
(1)
Porcine
(1)
Primate
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 68. Show All 68 Publications.
Publications using NB600-936 Applications Species
Bocek I, Hok L, Persoons L et al. Imidazo[4,5-b]pyridine derived tubulin polymerization inhibitors: Design, synthesis, biological activity in vitro and computational analysis Bioorganic chemistry Jul 16 2022 [PMID: 35872398] (WB, Human) WB Human
Castle AR, Wohlgemuth S, Arce L, Westaway D Investigating CRISPR/Cas9 gene drive for production of disease-preventing prion gene alleles PloS one Jun 7 2022 [PMID: 35671288] (WB, Mouse) WB Mouse
Wang LH, Lin CY, Lin YM et al. Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration The Journal of neuroscience : the official journal of the Society for Neuroscience Jun 22 2022 [PMID: 35606145]
SchAfer A, Evers L, Meier L et al. The Metalloprotease-Disintegrin ADAM8 Alters the Tumor Suppressor miR-181a-5p Expression Profile in Glioblastoma Thereby Contributing to Its Aggressiveness Frontiers in Oncology Mar 15 2022 [PMID: 35371977] (WB) WB
yang C, Dominique GM, Champion MM, Huber PW Remnants of the Balbiani body are required for formation of RNA transport granules in Xenopus oocytes iScience Mar 18 2022 [PMID: 35243240] (Xenopus) Xenopus
Cook L, Sengelmann M, Winkler B et al. ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9 International journal of molecular sciences Feb 10 2022 [PMID: 35216088] (WB, Human) WB Human
Clabough E, Ingersoll J, Reekes T et al. Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum International journal of molecular sciences Dec 28 2021 [PMID: 35008713] (Simple Western, Mouse) Simple Western Mouse
Zhao K, SchAfer A, Zhang Z et al. Inhibition of Carbonic Anhydrase 2 Overcomes Temozolomide Resistance in Glioblastoma Cells International Journal of Molecular Sciences Dec 23 2021 [PMID: 35008590] (WB) WB
Tristan C, Ormanoglu P, Slamecka J et al. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells Stem Cell Reports Dec 3 2021 [PMID: 34861164]
Della-Flora Nunes G, Wilson ER, Hurley E et al. Activation of mTORC1 and c-Jun by Prohibitin1 loss in Schwann cells may link mitochondrial dysfunction to demyelination eLife Sep 14 2021 [PMID: 34519641] (WB, Rat) WB Rat
Show All 68 Publications.

Reviews for beta Tubulin Antibody (NB600-936) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Ae. aegypti, Other.

Reviews using NB600-936:
Filter by Applications
WB
(4)
Simple Western
(1)
ICC
(1)
IHC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(4)
Ae. aegypti
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot beta Tubulin NB600-936
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4
reviewed by:
Verified Customer
WB Ae. aegypti 05/22/2021
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Summary

ApplicationWestern Blot
Sample TestedA549 Nuclear and cytoplasmic, Sample Amount: 30ug,Midgut tissue,Ovary tissue
SpeciesAe. aegypti
LotK

Comments

CommentsTo test both anti-histone H3 and anti-beta tubulin as markers for nuclear or cytoplasmic fractions, respectively, in Ae. aegypti mosquitoes, a western blot was performed using midguts or ovaries dissected from mosquitoes 48h post-bloodmeal. The cytoplasmic or nuclear fractions from each tissue type were obtained and protein concentration was quantified by A260/280. Roughly 30ug of tissue was loaded onto SDS/PAGE gels that were either transferred to PVDF membranes or stained by Coomassie. Membranes were blocked in TBST 5% milk for 2h. Both anti-histone H3 and anti-beta tubulin were added 1:1000 in blocking buffer and incubated O/N shaking at 4C. Proteins were detected by Alkaline Phosphatase. In Ae. aegypti ovaries, anti-histone H3 bound to protein roughly 15kDa in the nuclear fraction of ovaries but not the cytoplasmic fraction, while anti-beta-tubulin bound to protein roughly 55kDa in both cytoplasmic and nuclear fractions. In Ae. aegypti midguts, anti-histone H3 bound to 3 proteins between 10-15kDa strongly in the nuclear fraction and faintly in the cytoplasmic fraction-perhaps due to nuclear contamination in the cytoplasmic fraction. anti-beta-tubulin did not react well in midgut fractions. The beta-tubulin antibody was not successful for differentiating cytoplasmic from nuclear fractions in Ae. aegypti tissues. For your reference, protein ladder = pageruler plus prestained ThermoFisher
Simple Western beta Tubulin NB600-936
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3
reviewed by:
Anonymous -
Simple Western Mouse 01/21/2016
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Summary

ApplicationSimple Western
Sample Testedwhole brain homogenates from mice
SpeciesMouse
Western Blot beta Tubulin NB600-936
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4
reviewed by:
Verified Customer
WB Mouse 07/01/2014
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Summary

ApplicationWestern Blot
SpeciesMouse
Immunocytochemistry beta Tubulin NB600-936
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5
reviewed by:
Verified Customer
ICC Mouse 06/12/2014
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Summary

ApplicationImmunocytochemistry
SpeciesMouse
Western Blot beta Tubulin NB600-936
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5
reviewed by:
Verified Customer
WB Human 05/27/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
Immunohistochemistry beta Tubulin NB600-936
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5
reviewed by:
Lin Yi-Wen
IHC Mouse 01/13/2012
View

Summary

ApplicationImmunohistochemistry
Sample TestedHippocampal Primary Culture
SpeciesMouse
  5
reviewed by:
Hsien Sheng Tin
WB Other 11/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell line
SpeciesOther

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Tubulin Antibody (NB600-936). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for an antibody which is a specific marker for Mouse Bronchial cilia in IHC(p) technique. Preferably not from a mouse host. Can you help?
    • According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459

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Recent Reviews

4.4
5
4
4
2
3
1
2
0
1
0

Verified Customer
05/22/2021
Application: WB
Species: Ae. aegypti

Anonymous -
01/21/2016
Application: Simple Western
Species: Mouse

Verified Customer
07/01/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TUBB
Entrez
Uniprot