beta Tubulin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000
Western Blot: beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western blots showing Nox1 and Beta-tubulin in PC-3 cells or Nox4 and Beta-tubulin in ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) ...read more
Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.
Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

beta Tubulin Antibody Summary

Immunogen
A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot: P07437]
Localization
Major constituent of microtubules
Marker
Microtubule Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:1000 - 1:2000
  • Immunohistochemistry-Paraffin 1:1000 - 1:2000
  • Single Cell Western 1:40
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. SCW validated using murine pancreatic cancer cells.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
beta Tubulin Overexpression Lysate
Reviewed Applications
Read 7 Reviews rated 4.4
using
NB600-936 in the following applications:

Publications
Read Publications using
NB600-936 in the following applications:

  • 1 publication
  • 5 publications
  • WB
    33 publications

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 28114363). C. elegans reactivity reported in scientific literature (PMID: 27690361). Insect reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta Tubulin Antibody

  • beta polypeptide
  • MGC117247
  • Tubb5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Background

The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments, and tubulins comprises the microtubule building block. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6, b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Tr, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KO
AF-258-PB
Species: Hu
Applications: WB, Simple Western, IHC
NB600-936
Species: Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, Single-Cell Western

Publications for beta Tubulin Antibody (NB600-936)(52)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Invertebrate, Porcine, Primate.

We have publications tested in 3 applications: Flow, ICC/IF, WB.


Filter By Application
Flow
(1)
ICC/IF
(5)
WB
(33)
All Applications
Filter By Species
Human
(16)
Mouse
(12)
Rat
(10)
Bovine
(1)
Invertebrate
(1)
Porcine
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 52. Show All 52 Publications.
Publications using NB600-936 Applications Species
Chen Y, Tristan CA, Lu C et al. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells biorxiv 2019 Oct 22 (WB, Human) WB Human
Hatzopoulos GN, KUkenshOner T, Banterle N et al. Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly Nature communications Jun 21 2021 [PMID: 34155202] (WB, Human) WB Human
Della-Flora Nunes G, Wilson ER, Marziali LN et al. Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells Nature communications Jun 2 2021 [PMID: 34078899] (WB, Mouse) WB Mouse
Wu YH, Yang Y, Chen CH et al. Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2 PLoS pathogens Mar 1 2021 [PMID: 33720996] (WB, Human) WB Human
Tristan CA, Ormanoglu P, Slamecka J et al. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells bioRxiv Aug 3 2020 [PMID: 32793899] (WB, Human) WB Human
Wang H, Zhang Z, Xie X et al. Paracellular pathway-mediated Mycoplasma hyopneumoniae migration across porcine airway epithelial barrier under air-liquid interface conditions Infect. Immun. Aug 3 2020 [PMID: 32747599]
Reed CB, Frick LR, Weaver A et al. Deletion of Calcineurin in Schwann Cells Does Not Affect Developmental Myelination, But Reduces Autophagy and Delays Myelin Clearance after Peripheral Nerve Injury J. Neurosci. Jul 8 2020 [PMID: 32641402]
Weinstock NI, Shin D, Dhimal N et al. Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction Neuron Apr 28 2020 [PMID: 32375064]
Contractor T, Harris C Loss of copy of MIR1-2 increases CDK4 expression in ileal neuroendocrine tumors. Oncogenesis Mar 20 2020 [PMID: 32198354] (Flow, Human) Flow Human
Sun R, Eriksson S, Wang L The expression and activity of thymidine kinase 1 and deoxycytidine kinase are modulated by hydrogen peroxide and nucleoside analogs Nucleosides Nucleotides Nucleic Acids Mar 19 2020 [PMID: 32189555]
Show All 52 Publications.

Reviews for beta Tubulin Antibody (NB600-936) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Ae. aegypti, Other.

Reviews using NB600-936:
Filter by Applications
WB
(4)
Simple Western
(1)
ICC
(1)
IHC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(4)
Ae. aegypti
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot beta Tubulin NB600-936
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4
reviewed by:
Adeline Williams
WB Ae. aegypti 05/22/2021
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Summary

ApplicationWestern Blot
Sample TestedA549 Nuclear and cytoplasmic, Sample Amount: 30ug,Midgut tissue,Ovary tissue
SpeciesAe. aegypti
LotK

Comments

CommentsTo test both anti-histone H3 and anti-beta tubulin as markers for nuclear or cytoplasmic fractions, respectively, in Ae. aegypti mosquitoes, a western blot was performed using midguts or ovaries dissected from mosquitoes 48h post-bloodmeal. The cytoplasmic or nuclear fractions from each tissue type were obtained and protein concentration was quantified by A260/280. Roughly 30ug of tissue was loaded onto SDS/PAGE gels that were either transferred to PVDF membranes or stained by Coomassie. Membranes were blocked in TBST 5% milk for 2h. Both anti-histone H3 and anti-beta tubulin were added 1:1000 in blocking buffer and incubated O/N shaking at 4C. Proteins were detected by Alkaline Phosphatase. In Ae. aegypti ovaries, anti-histone H3 bound to protein roughly 15kDa in the nuclear fraction of ovaries but not the cytoplasmic fraction, while anti-beta-tubulin bound to protein roughly 55kDa in both cytoplasmic and nuclear fractions. In Ae. aegypti midguts, anti-histone H3 bound to 3 proteins between 10-15kDa strongly in the nuclear fraction and faintly in the cytoplasmic fraction-perhaps due to nuclear contamination in the cytoplasmic fraction. anti-beta-tubulin did not react well in midgut fractions. The beta-tubulin antibody was not successful for differentiating cytoplasmic from nuclear fractions in Ae. aegypti tissues. For your reference, protein ladder = pageruler plus prestained ThermoFisher
Simple Western beta Tubulin NB600-936
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3
reviewed by:
Verified Customer
Simple Western Mouse 01/21/2016
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Summary

ApplicationSimple Western
Sample Testedwhole brain homogenates from mice
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1:10,000, 1:5,000, 1:1,000

Details

Detection NotesWes Simple Western platform

Comments

CommentsBest at lower concentrations due to some faint high molecular weight bands
Western Blot beta Tubulin NB600-936
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4
reviewed by:
Liyong Zhang
WB Mouse 07/01/2014
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Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF
Immunocytochemistry beta Tubulin NB600-936
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5
reviewed by:
Anony- mous
ICC Mouse 06/12/2014
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Summary

ApplicationImmunocytochemistry
SpeciesMouse
Special ApplicationsConfocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot beta Tubulin NB600-936
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5
reviewed by:
Anony- mous
WB Human 05/27/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF
Immunohistochemistry beta Tubulin NB600-936
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5
reviewed by:
Verified Customer
IHC Mouse 01/13/2012
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Summary

ApplicationImmunohistochemistry
Sample TestedHippocampal Primary Culture
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Jackson / Goat anti Rabbit IgG DyLight488, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Visualization Method: Microscope
  5
reviewed by:
Verified Customer
WB Other 11/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell line
SpeciesOther
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse HRT

Details

Detection NotesDetection Method: ECL

Comments

CommentsGood!

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Tubulin Antibody (NB600-936). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for an antibody which is a specific marker for Mouse Bronchial cilia in IHC(p) technique. Preferably not from a mouse host. Can you help?
    • According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-936AF350
Alexa Fluor 405 NB600-936AF405
Alexa Fluor 488 NB600-936AF488
Alexa Fluor 532 NB600-936AF532
Alexa Fluor 594 NB600-936AF594
Alexa Fluor 647 NB600-936AF647
Alexa Fluor 700 NB600-936AF700
Alexa Fluor 750 NB600-936AF750
Biotin NB600-936B
DyLight 350 NB600-936UV
DyLight 405 NB600-936V
DyLight 488 NB600-936G
DyLight 550 NB600-936R
DyLight 594 NB600-936DL594
DyLight 650 NB600-936C
DyLight 680 NB600-936FR
DyLight 755 NB600-936IR
HRP NB600-936H
Janelia Fluor 549 NB600-936JF549
Janelia Fluor 646 NB600-936JF646

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Recent Reviews

4.4
5
4
4
2
3
1
2
0
1
0

Adeline Williams
05/22/2021
Application: WB
Species: Ae. aegypti

Verified Customer
01/21/2016
Application: Simple Western
Species: Mouse

Liyong Zhang
07/01/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TUBB
Entrez
Uniprot