beta Tubulin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000

Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western blots showing Nox1 and Beta-tubulin in PC-3 cells or Nox4 and Beta-tubulin in C2C12 cells, at 5% and 18% O2. (b, c) Average Nox1 signal How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30363917/) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification].

Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.

Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin and TATA binding protein (TBP) were used as protein markers for the cytosol and nucleus fraction, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep29311), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification].

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification].

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification].

• Reactivity: Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, I, In, Pm, Xp, Ze
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

beta Tubulin Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot: P07437]
• Localization: Major constituent of microtubules
• Marker: Microtubule Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:1000 - 1:2000
  • Immunohistochemistry-Paraffin 1:1000 - 1:2000
  • Simple Western 1:1000
  • Western Blot 1:1000
• Application Notes: Use in Simple Western reported in scientific literature (PMID:35008713) In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. SCW validated using murine pancreatic cancer cells.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  beta Tubulin Overexpression Lysate

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34519641). Invertebrate reactivity reported in scientific literature (PMID: 28114363). C. elegans reactivity reported in scientific literature (PMID: 27690361). Insect reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta Tubulin Antibody - BSA Free

• beta polypeptide
• beta Tubulin
• MGC117247
• OK/SW-cl.56
• TUBB
• TUBB5
• tubulin beta chain
• tubulin beta polypeptide
• tubulin beta-1 chain
• Tubulin beta-5 chain
• tubulin, beta

Background

The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments, and tubulins comprises the microtubule building block. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6 , b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Tubulin Antibody (NB600-936)(71)

Publications using NB600-936

• D'Otolo V Functional complementarity of mammalian coronins determines T cell population homeostasis Thesis 2022-01-01 (WB, Human)
• Kang S, Han Z, Daude N et al. Tau conformers in FTLD-MAPT undergo liquid-liquid phase separation and perturb the nuclear envelope bioRxiv 2020-07-06 (WB, Human)
• Robbins L, Balaram A, Dejneka S et al. Heterologous Production of the D-Cycloserine Intermediate O-acetyl-L-serine in Human Lung Cancer Cells Research Square 2022-10-24 (WB, Human)
• Nunes GDF Elucidating the role of Schwann cell mitochondria in myelin maintenance Thesis 2021-01-01
• Li X, Su Q, Pan Y Overcharged lipid metabolism in mechanisms of antitumor by Tremella fuciformis‑derived polysaccharide International journal of oncology 2023-01-01 [PMID: 36416344] (WB, Mouse)
  
  Details:
  Dilutions: 1:1000
• Shackleford G, Marziali LN, Sasaki Y Et al. A new mouse model of Charcot-Marie-Tooth 2J neuropathy replicates human axonopathy and suggest alteration in axo-glia communication PLoS Genet 2022-11-09 [PMID: 36350884] (WB, Mouse, Primate)
  
  Details:
  Citation using the HRP version of this antibody.
• Bocek I, Hok L, Persoons L et al. Imidazo[4,5-b]pyridine derived tubulin polymerization inhibitors: Design, synthesis, biological activity in vitro and computational analysis Bioorganic chemistry 2022-07-16 [PMID: 35872398] (WB, Human)
• Castle AR, Wohlgemuth S, Arce L, Westaway D Investigating CRISPR/Cas9 gene drive for production of disease-preventing prion gene alleles PloS one 2022-06-07 [PMID: 35671288] (WB, Mouse)
• Wang LH, Lin CY, Lin YM et al. Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration The Journal of neuroscience : the official journal of the Society for Neuroscience 2022-06-22 [PMID: 35606145]
• SchAfer A, Evers L, Meier L et al. The Metalloprotease-Disintegrin ADAM8 Alters the Tumor Suppressor miR-181a-5p Expression Profile in Glioblastoma Thereby Contributing to Its Aggressiveness Frontiers in Oncology 2022-03-15 [PMID: 35371977] (WB)
• yang C, Dominique GM, Champion MM, Huber PW Remnants of the Balbiani body are required for formation of RNA transport granules in Xenopus oocytes iScience 2022-03-18 [PMID: 35243240] (Xenopus)
• Cook L, Sengelmann M, Winkler B et al. ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9 International journal of molecular sciences 2022-02-10 [PMID: 35216088] (WB, Human)
• Clabough E, Ingersoll J, Reekes T et al. Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum International journal of molecular sciences 2021-12-28 [PMID: 35008713] (Simple Western, Mouse)
• Zhao K, SchAfer A, Zhang Z et al. Inhibition of Carbonic Anhydrase 2 Overcomes Temozolomide Resistance in Glioblastoma Cells International Journal of Molecular Sciences 2021-12-23 [PMID: 35008590] (WB)
• Tristan C, Ormanoglu P, Slamecka J et al. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells Stem Cell Reports 2021-12-03 [PMID: 34861164]
• Della-Flora Nunes G, Wilson ER, Hurley E et al. Activation of mTORC1 and c-Jun by Prohibitin1 loss in Schwann cells may link mitochondrial dysfunction to demyelination eLife 2021-09-14 [PMID: 34519641] (WB, Rat)
• Kang SG, Han ZZ, Daude N et al. Pathologic tau conformer ensembles induce dynamic, liquid-liquid phase separation events at the nuclear envelope BMC Biology 2021-12-01 [PMID: 34503506] (WB)
• Izadifar A, Courchet J, Virga DM Et al. Axon morphogenesis and maintenance require an evolutionary conserved safeguard function of Wnk kinases antagonizing Sarm and Axed Neuron 2021-08-04 [PMID: 34384519] (WB)
• Hatzopoulos GN, KUkenshOner T, Banterle N et al. Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly Nature communications 2021-06-21 [PMID: 34155202] (WB, Human)
• Della-Flora Nunes G, Wilson ER, Marziali LN et al. Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells Nature communications 2021-06-02 [PMID: 34078899] (WB, Mouse)
• Chen Y, Tristan CA, Lu C et al. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells Nat Methods 2021-05-04 [PMID: 33941937]
• Wu YH, Yang Y, Chen CH et al. Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2 PLoS pathogens 2021-03-01 [PMID: 33720996] (WB, Human)
• Tristan CA, Ormanoglu P, Slamecka J et al. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells bioRxiv 2020-08-03 [PMID: 32793899] (WB, Human)
• Wang H, Zhang Z, Xie X et al. Paracellular pathway-mediated Mycoplasma hyopneumoniae migration across porcine airway epithelial barrier under air-liquid interface conditions Infect. Immun. 2020-08-03 [PMID: 32747599]
• Reed CB, Frick LR, Weaver A et al. Deletion of Calcineurin in Schwann Cells Does Not Affect Developmental Myelination, But Reduces Autophagy and Delays Myelin Clearance after Peripheral Nerve Injury J. Neurosci. 2020-07-08 [PMID: 32641402]
• Weinstock NI, Shin D, Dhimal N et al. Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction Neuron 2020-04-28 [PMID: 32375064]
• Baxter, M, Chapagai, D Et al. Peptidomimetic Polo-Box-Targeted Inhibitors that Engage PLK1 in Tumor Cells and Are Selective against the PLK3 Tumor Suppressor. ChemMedChem 2020-06-17 [PMID: 32232973] (WB, Human)
• Contractor T, Harris C Loss of copy of MIR1-2 increases CDK4 expression in ileal neuroendocrine tumors. Oncogenesis 2020-03-20 [PMID: 32198354] (FLOW, Human)
• Sun R, Eriksson S, Wang L The expression and activity of thymidine kinase 1 and deoxycytidine kinase are modulated by hydrogen peroxide and nucleoside analogs Nucleosides Nucleotides Nucleic Acids 2020-03-19 [PMID: 32189555]
• Markovics A, Angyal A, et al. GPR119 Is a Potent Regulator of Human Sebocyte Biology. J Invest Dermatol 2020-10-01 [PMID: 32142797] (WB, Human)
• Beaulieu-Laroche L, Christin M, Donoghue A et al. TACAN Is an Ion Channel Involved in Sensing Mechanical Pain Cell 2020-02-17 [PMID: 32084332] (WB, Human)
• Kallupi M, Carrette LLG, Kononoff J et al. Nociceptin attenuates the escalation of oxycodone self-administration by normalizing CeA-GABA transmission in highly addicted rats Proc. Natl. Acad. Sci. U.S.A. 2020-01-28 [PMID: 31932450] (WB, Rat)
• Ridley RB, Young BM, Lee J et al. AAV Mediated Delivery of Myxoma Virus M013 Gene Protects the Retina against Autoimmune Uveitis J Clin Med 2019-11-29 [PMID: 31795515] (WB, Mouse)
• Li, Y, Xing, Q Et al. Activation of RXR by bexarotene inhibits inflammatory conditions in human rheumatoid arthritis fibroblast like synoviocytes. Int J Mol Med 2019-11-01 [PMID: 31545398] (WB, Human)
• Gyongyosi M, Lukovic D, Zlabinger K et al. Liposomal doxorubicin attenuates cardiotoxicity via induction of interferon-related DNA damage resistance Cardiovasc. Res. 2019-07-25 [PMID: 31346605] (WB, Porcine)
• Hannen R, Selmansberger M, Hauswald M et al. Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor Cancers (Basel) 2019-06-30 [PMID: 31262047] (WB, Human)
• Scharfenberg F, Helbig A, Sammel M et al. Degradome of soluble ADAM10 and ADAM17 metalloproteases Cell. Mol. Life Sci. 2019-06-17 [PMID: 31209506]
• Baraliakos X, Boehm H, Bahrami R et al. Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload Neuroscience 2019-05-23 [PMID: 31129202] (ICC/IF, Rat)
• Lee BJ, Jun HO, Kim JH, Kim JH. Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina FASEB J. 2019-02-11 [PMID: 30742774] (WB, Human)
• Castle AR, Daude N, Gilch S, Westaway D. Application of high-throughput, capillary-based Western analysis to modulated cleavage of the cellular prion protein J. Biol. Chem. 2018-12-21 [PMID: 30578300] (WB, Mouse)
• Hannen R, Steffani M, Voellger B et al. Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study. J. Steroid Biochem. Mol. Biol. 2018-11-13 [PMID: 30439415] (WB, Rat)
• Stuart JA, Fonseca J, Moradi F et al. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxid Med Cell Longev. 2018-09-30 [PMID: 30363917] (WB, Human, Mouse)
• Yang L, Chen L, Lu X et al. Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice Reproduction 2018-05-29 [PMID: 29844226] (WB, Mouse)
• Hernandez M. R, Davis M. B, et al. Condensin I protects meiotic cohesin from WAPL-1 mediated removal. PLoS Genet 2018-05-01 [PMID: 29768402] (WB, C. elegans)
• Mao Z, Yang L, Lu X, Tan A. C1QTNF3 in the murine ovary and its function in folliculogenesis. Reproduction. 2018-02-01 [PMID: 29438034] (WB, Mouse)
• Schulte ML, Fu A, Zhao P et al. Pharmacological blockade of ASCT2-dependent glutamine transport leads to antitumor efficacy in preclinical models. Nat. Med. 2018-02-01 [PMID: 29334372] (WB, Human)
• Bengoetxea H, Rico-Barrio I, Ortuzar N et al. Environmental Enrichment Reverses Tyrosine Kinase Inhibitor-Mediated Impairment Through BDNF-TrkB Pathway Mol. Neurobiol. 2017-08-25 [PMID: 28842826] (Rat)
• Requejo C, Ruiz-Ortega JA, Bengoetxea H et al. Deleterious Effects of VEGFR2 and RET Inhibition in a Preclinical Model of Parkinson's Disease Mol. Neurobiol. 2017-08-24 [PMID: 28840516] (Rat)
• Requejo C, Ruiz-Ortega JA, Cepeda H et al. Nanodelivery of Cerebrolysin and Rearing in Enriched Environment Induce Neuroprotective Effects in a Preclinical Rat Model of Parkinson's Disease Mol. Neurobiol. 2017-08-24 [PMID: 28840482] (Rat)
• Gelmedin V, Morel M, Hahnel S et al. Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival. PLoS Pathog [PMID: 28114363] (ICC/IF, Invertebrate)
• Vercruysse T, De Bie J, et al. The Second-Generation Exportin-1 Inhibitor KPT-8602 Demonstrates Potent Activity against Acute Lymphoblastic Leukemia. Clin Cancer Res 2017-05-15 [PMID: 27780859] (SW, Human)
• Lau AC, Zhu KP, Brouhard EA et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin. 2016-10-25 [PMID: 27777629] (WB)
• Snyder MJ, Lau AC, Brouhard EA et al. Anchoring of Heterochromatin to the Nuclear Lamina Reinforces Dosage Compensation-Mediated Gene Repression. PLoS Genet. 2016-09-01 [PMID: 27690361]
• Lee MC, Huang HJ, Chang TH et al. Genome-wide analysis of HIF-2alpha chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions. Sci Rep 2016-07-04 [PMID: 27373565] (WB)
• Ali AA, Lee YR, Chen TC et al. Novel Anthra[1,2-c][1,2,5]Thiadiazole-6,11-Diones as Promising Anticancer Lead Compounds: Biological Evaluation, Characterization & Molecular Targets Determination. PLoS One 2016-04-21 [PMID: 27100886] (WB)
• Scott M R, Rubio M D et al. Protein Expression of Proteasome Subunits in Elderly Patients with Schizophrenia. Neuropsychopharmacology 2016-01-02 [PMID: 26202105] (WB, Rat)
• McKinley ET, Zhao P, Coffey RJ et al. 3'-Deoxy-3'-[18F]-Fluorothymidine PET Imaging Reflects PI3K-mTOR-Mediated Pro-Survival Response to Targeted Therapy in Colorectal Cancer. PLoS ONE. 2014-09-24 [PMID: 25247710] (WB, Mouse)
• Chen WH, Cheng SJ, Tzen JT et al. Probing Relevant Molecules in Modulating the Neurite Outgrowth of Hippocampal Neurons on Substrates of Different Stiffness. PLoS One 2013-12-30 [PMID: 24386192] (ICC/IF, Mouse)
• Miklosz A, Lukaszuk B, Baranowski M et al. Effects of Inhibition of Serine Palmitoyltransferase (SPT) and Sphingosine Kinase 1 (SphK1) on Palmitate Induced Insulin Resistance in L6 Myotubes. PLoS One 2013-12-23 [PMID: 24376889] (WB, Rat)
• Lu ZH, Kaliberov S, Sohn R et al. Transcriptional Targeting of Primary and Metastatic Tumor Neovasculature by an Adenoviral Type 5 Roundabout4 Vector in Mice. PLoS One 2013-12-23 [PMID: 24376772] (WB, Mouse)
• Orlicky DJ, Monks J, Stefanski AL, McManaman JL. Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion. PLoS One 2013-06-25 [PMID: 23825572]
• Riva M, He Z, Kallberg E et al. Human S100A9 Protein Is Stabilized by Inflammatory Stimuli via the Formation of Proteolytically-Resistant Homodimers. PLoS One 2013-04-23 [PMID: 23626736] (WB, Human, Primate)
• McKinley ET, Smith RA, Zhao P et al. 3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer. Chem Biol Interact. 2013-03-25 [PMID: 23341544] (WB, Human)
• Jacek E, Fallon BA, Chandra A et al. Increased IFNalpha activity and differential antibody response in patients with a history of Lyme disease and persistent cognitive deficits J Neuroimmunol 2012-11-07 [PMID: 23141748] (WB, Mouse)
• Li Z, Zhao ZJ, Zhu XQ, Ren QS, Nie FF, Gao JM, Gao XJ, Yang TB, Zhou WL, Shen JL, Wang Y, Lu FL, Chen XG, Hide G, Ayala FJ, Lun ZR. Differences in iNOS and Arginase Expression and Activity in the Macrophages of Rats Are Responsible for the Resistance against T gondii Infection. PLoS One;7(4). 2012-01-01 [PMID: 22558235] (WB, Mouse, Rat)
• Kaucher AV, Oatley MJ, Oatley JM. NEUROG3 is a critical downstream effector for STAT3-regulated differentiation of mammalian stem and progenitor spermatogonia Biol Reprod 2012-05-31 [PMID: 22378757] (WB, Mouse)
• Kadiu I, Gendelman HE. Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection J Neuroimmune Pharmacol 2011-12-01 [PMID: 21789505] (ICC/IF, Human)
• Lin SY, Chen NT, Sun SP et al. The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death. J Am Chem Soc. 2010-06-23 [PMID: 20499915] (WB, Human)
• Reding SC, Stepnoski AL, Cloninger EW, Oatley JM. THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis. Reproduction;139(5):893-903. 2010-05-01 [PMID: 20154176] (WB, Bovine)
• Catalucci D, Zhang D-H, DeSantiago J et al. Akt regulates L-type Ca2+ channel activity by modulating Cav{alpha}1 protein stability. J Cell Biol;184(6):923-933. 2009-01-01 [PMID: 19307602]
• Rattner A, Toulabi L, Williams J et al. The genomic response of the retinal pigment epithelium to light damage and retinal detachment. J Neurosci. 2008-01-01 [PMID: 18815272]

Reviews for beta Tubulin Antibody (NB600-936) (7)

Average Rating: 4.4

(Based on 7 reviews)

We have 7 reviews tested in 4 species: Human, Mouse, Ae. aegypti, Other.

reviewed by: Verified Customer WB Ae. aegypti 05/22/2021

Summary

• Application: Western Blot
• Sample Tested: A549 Nuclear and cytoplasmic, Sample Amount: 30ug,Midgut tissue,Ovary tissue
• Species: Ae. aegypti
• Lot: K

Comments

• Comments: To test both anti-histone H3 and anti-beta tubulin as markers for nuclear or cytoplasmic fractions, respectively, in Ae. aegypti mosquitoes, a western blot was performed using midguts or ovaries dissected from mosquitoes 48h post-bloodmeal. The cytoplasmic or nuclear fractions from each tissue type were obtained and protein concentration was quantified by A260/280. Roughly 30ug of tissue was loaded onto SDS/PAGE gels that were either transferred to PVDF membranes or stained by Coomassie. Membranes were blocked in TBST 5% milk for 2h. Both anti-histone H3 and anti-beta tubulin were added 1:1000 in blocking buffer and incubated O/N shaking at 4C. Proteins were detected by Alkaline Phosphatase. In Ae. aegypti ovaries, anti-histone H3 bound to protein roughly 15kDa in the nuclear fraction of ovaries but not the cytoplasmic fraction, while anti-beta-tubulin bound to protein roughly 55kDa in both cytoplasmic and nuclear fractions. In Ae. aegypti midguts, anti-histone H3 bound to 3 proteins between 10-15kDa strongly in the nuclear fraction and faintly in the cytoplasmic fraction-perhaps due to nuclear contamination in the cytoplasmic fraction. anti-beta-tubulin did not react well in midgut fractions. The beta-tubulin antibody was not successful for differentiating cytoplasmic from nuclear fractions in Ae. aegypti tissues. For your reference, protein ladder = pageruler plus prestained ThermoFisher

reviewed by: Anonymous - Simple Western Mouse 01/21/2016

Summary

• Application: Simple Western
• Sample Tested: whole brain homogenates from mice
• Species: Mouse

reviewed by: Verified Customer WB Mouse 07/01/2014

Summary

• Application: Western Blot
• Species: Mouse

reviewed by: Verified Customer ICC Mouse 06/12/2014

Summary

• Application: Immunocytochemistry
• Species: Mouse

reviewed by: Verified Customer WB Human 05/27/2014

Summary

• Application: Western Blot
• Species: Human

reviewed by: Lin Yi-Wen IHC Mouse 01/13/2012

Summary

• Application: Immunohistochemistry
• Sample Tested: Hippocampal Primary Culture
• Species: Mouse

reviewed by: Hsien Sheng Tin WB Other 11/09/2011

Summary

• Application: Western Blot
• Sample Tested: Chicken cell line
• Species: Other

FAQs for beta Tubulin Antibody (NB600-936). (Showing 1 - 1 of 1 FAQs).

1. We are looking for an antibody which is a specific marker for Mouse Bronchial cilia in IHC(p) technique. Preferably not from a mouse host. Can you help?
   • According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459

Bioinformatics

• Gene Symbol: TUBB
• Entrez:
  • 203068(Human)
  • 545486(Mouse)
  • 498736(Rat)
• Uniprot:
  • P07437(Human)