beta Tubulin Antibody

Images

 
Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) ...read more
Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen.
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative ...read more
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong ...read more
Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ce, Ch, ChHa, In, Pm, Xp, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, Single Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

beta Tubulin Antibody Summary

Immunogen
A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot: P07437]
Localization
Major constituent of microtubules
Marker
Microtubule Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:1000 - 1:2000
  • Immunohistochemistry-Paraffin 1:1000 - 1:2000
  • Single Cell Western 1:40
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
beta Tubulin Overexpression Lysate
Reviewed Applications
Read 6 Reviews rated 4.5
using
NB600-936 in the following applications:

Publications
Read Publications using
NB600-936 in the following applications:

  • 4 publications
  • WB
    22 publications

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 28114363). C. elegans reactivity reported in scientific literature (PMID: 27690361).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta Tubulin Antibody

  • beta polypeptide
  • MGC117247
  • Tubb5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Background

The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments, and tubulins comprises the microtubule building block. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6, b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta Tubulin Antibody (NB600-936)(34)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Invertebrate, Porcine, Primate.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(4)
WB
(22)
All Applications
Filter By Species
Human
(8)
Mouse
(10)
Rat
(7)
Bovine
(1)
Invertebrate
(1)
Porcine
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB600-936 Applications Species
Gyongyosi M, Lukovic D, Zlabinger K et al. Liposomal doxorubicin attenuates cardiotoxicity via induction of interferon-related DNA damage resistance Cardiovasc. Res. Jul 25 2019 [PMID: 31346605] (WB, Porcine) WB Porcine
Hannen R, Selmansberger M, Hauswald M et al. Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor Cancers (Basel) Jun 30 2019 [PMID: 31262047] (WB, Human) WB Human
Scharfenberg F, Helbig A, Sammel M et al. Degradome of soluble ADAM10 and ADAM17 metalloproteases Cell. Mol. Life Sci. Jun 17 2019 [PMID: 31209506]
Baraliakos X, Boehm H, Bahrami R et al. Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload Neuroscience May 23 2019 [PMID: 31129202] (ICC/IF, Rat) ICC/IF Rat
Lee BJ, Jun HO, Kim JH, Kim JH. Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina FASEB J. Feb 11 2019 [PMID: 30742774] (WB, Human) WB Human
Castle AR, Daude N, Gilch S, Westaway D. Application of high-throughput, capillary-based Western analysis to modulated cleavage of the cellular prion protein J. Biol. Chem. Dec 21 2018 [PMID: 30578300] (WB, Mouse) WB Mouse
Hannen R, Steffani M, Voellger B et al. Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study. J. Steroid Biochem. Mol. Biol. Nov 13 2018 [PMID: 30439415] (WB, Rat) WB Rat
Stuart JA, Fonseca J, Moradi F et al. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxid Med Cell Longev. Sep 30 2018 [PMID: 30363917] (WB, Human, Mouse) WB Human, Mouse
Yang L, Chen L, Lu X et al. Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice Reproduction May 29 2018 [PMID: 29844226] (WB, Mouse) WB Mouse
Mao Z, Yang L, Lu X, Tan A. C1QTNF3 in the murine ovary and its function in folliculogenesis. Reproduction. 2018 Feb 01 [PMID: 29438034] (WB, Mouse) WB Mouse
Show All 34 Publications.

Reviews for beta Tubulin Antibody (NB600-936) (6) 4.56

Average Rating: 4.5
(Based on 6 reviews)
We have 6 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NB600-936:
Filter by Applications
Simple Western
(1)
WB
(3)
ICC
(1)
IHC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western beta Tubulin NB600-936
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reviewed by:
Anonymous
Simple Western Mouse 01/21/2016
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Summary

ApplicationSimple Western
Sample Testedwhole brain homogenates from mice
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1:10,000, 1:5,000, 1:1,000

Details

Detection NotesWes Simple Western platform

Comments

CommentsBest at lower concentrations due to some faint high molecular weight bands
Western Blot beta Tubulin NB600-936
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reviewed by:
Liyong Zhang
WB Mouse 07/01/2014
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Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF
Immunocytochemistry beta Tubulin NB600-936
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reviewed by:
Anony- mous
ICC Mouse 06/12/2014
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Summary

ApplicationImmunocytochemistry
SpeciesMouse
Special ApplicationsConfocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot beta Tubulin NB600-936
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reviewed by:
Anony- mous
WB Human 05/27/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF
Immunohistochemistry beta Tubulin NB600-936
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reviewed by:
Anonymous
IHC Mouse 01/13/2012
View

Summary

ApplicationImmunohistochemistry
Sample TestedHippocampal Primary Culture
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Jackson / Goat anti Rabbit IgG DyLight488, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Visualization Method: Microscope
 
reviewed by:
Anonymous
WB Other 11/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedChicken cell line
SpeciesOther
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse HRT

Details

Detection NotesDetection Method: ECL

Comments

CommentsGood!

Product General Protocols

View specific protocols for beta Tubulin Antibody (NB600-936): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Tubulin Antibody (NB600-936). (Showing 1 - 2 of 2 FAQs).

  1. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.
  2. We are looking for an antibody which is a specific marker for Mouse Bronchial cilia in IHC(p) technique. Preferably not from a mouse host. Can you help?
    • According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-936AF350
Alexa Fluor 405 NB600-936AF405
Alexa Fluor 488 NB600-936AF488
Alexa Fluor 532 NB600-936AF532
Alexa Fluor 594 NB600-936AF594
Alexa Fluor 647 NB600-936AF647
Alexa Fluor 700 NB600-936AF700
Alexa Fluor 750 NB600-936AF750
Biotin NB600-936B
DyLight 350 NB600-936UV
DyLight 405 NB600-936V
DyLight 488 NB600-936G
DyLight 550 NB600-936R
DyLight 594 NB600-936DL594
DyLight 650 NB600-936C
DyLight 680 NB600-936FR
DyLight 755 NB600-936IR
HRP NB600-936H
Janelia Fluor 549 NB600-936JF549
Janelia Fluor 646 NB600-936JF646

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Recent Reviews

4.5
5
4
4
1
3
1
2
0
1
0

Anonymous
01/21/2016
Application: Simple Western
Species: Mouse

Liyong Zhang
07/01/2014
Application: WB
Species: Mouse

Anony- mous
06/12/2014
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol TUBB
Entrez
Uniprot