ARX Antibody

HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="ARX was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

ARX was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ARX Antibody Summary

• Immunogen: E. coli-derived recombinant human ARX
  Ser2-Ala100
  Accession # Q96QS3
• Specificity: Detects human ARX in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ARX
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARX Antibody

• aristaless related homeobox
• Aristaless-related homeobox
• aristaless-related homeobox, X-linked
• ARX
• cancer/testis antigen 121
• CT121
• EIEE1
• homeobox protein ARX
• ISSX
• ISSXmental retardation, X-linked 36
• mental retardation, X-linked 29
• mental retardation, X-linked 32
• mental retardation, X-linked 33
• mental retardation, X-linked 38
• mental retardation, X-linked 43
• mental retardation, X-linked 54
• mental retardation, X-linked 76
• mental retardation, X-linked 87
• MRX29
• MRX32
• MRX33
• MRX36
• MRX38
• MRX43
• MRX54
• MRX76
• MRX87
• MRXS1
• PRTS

Background

ARX (Aristaless-Related homeoboX protein) is a 56-62 kDa member of the aristaless subfamily, paired homeobox family of proteins. In the nervous system, it is expressed in embryonic telencephalon plus adult cortical GABAergic neurons and differentiating neuronal stem cells; in the pancreas, it promotes the differentiation of alpha -cells at the expense of beta -cells. Human ARX is a 562 amino acid (aa) transcriptional repressor. It contains an octapeptide motif that binds Groucho proteins (aa 27‑34), an NLS (aa 82-89), one Ala-rich region (aa 100-155), an acidic/Glu-rich region (aa 224-253), a DNA-binding homeodomain with an embedded NLS (aa 328‑387), one Pro-rich segment that binds CtBP1 (aa 395-459) and a C-terminal Aristaless domain (aa 530-543). There are mutations that show poly-Ala expansions in the N-terminus (aa 115-155), often associated with developmental abnormalities. Over aa 1-100, human ARX shares 97% aa identity with mouse ARX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARX Antibody (AF7068)(12)

Publications using AF7068

• Meng, G;Gu, J;Liew, SY;Cao, J;Wang, Z;Ma, C;Fu, Z;Zhou, H;Wang, J;Wang, S;Jing, S;Wu, Y;Lei, Z;Zhi, S;He, Y;Li, C;Deng, H; Reconstruction of endocrine subtype-complete human pluripotent stem cell-derived islets with capacity for hypoglycemia protection in vivo Cell stem cell 2025-08-02 [PMID: 40782792] (Flow Cytometry, Immunohistochemistry, Human, Xenograft)
• Jingcheng Zhang, Rui Jiang, Xiafei Hong, Huanwen Wu, Xianlin Han, Wenming Wu Metastatic insulinoma: exploration from clinicopathological signatures and genetic characteristics Frontiers in Oncology 5/12/2023 [PMID: 37251916]
• Sara J. Knowles, April M. Stafford, Tariq Zaman, Kartik Angara, Michael R. Williams, Jason M. Newbern et al. Distinct hyperactive RAS/MAPK alleles converge on common GABAergic interneuron core programs Development 2023-05-15 [PMID: 37166243] (Immunohistochemistry, Mouse)
• Tincy Simon, Pamela Riemer, Armin Jarosch, Katharina Detjen, Annunziata Di Domenico, Felix Bormann et al. DNA methylation reveals distinct cells of origin for pancreatic neuroendocrine carcinomas and pancreatic neuroendocrine tumors Genome Medicine 2022-03-01 [PMID: 35227293] (Immunohistochemistry, Human)
• Xiao-Qing Dai, Joan Camunas-Soler, Linford J.B. Briant, Theodore dos Santos, Aliya F. Spigelman, Emily M. Walker et al. Heterogenous impairment of alpha cell function in type 2 diabetes is linked to cell maturation state Cell Metabolism 2022-02-01 [PMID: 35108513]
• Rachana Haliyur, John T Walker, May Sanyoura, Conrad V Reihsmann, Shristi Shrestha, Radhika Aramandla et al. Integrated Analysis of the Pancreas and Islets Reveals Unexpected Findings in Human Male With Type 1 Diabetes Journal of the Endocrine Society 2021-12-01 [PMID: 34870058] (Immunohistochemistry, Human)
• Annunziata Di Domenico, Christodoulos P. Pipinikas, Renaud S. Maire, Konstantin Bräutigam, Cedric Simillion, Matthias S. Dettmer et al. Epigenetic landscape of pancreatic neuroendocrine tumours reveals distinct cells of origin and means of tumour progression Communications Biology 2020-12-07 [PMID: 33288854]
• X Shi, W Lin, X Gao, W Xie, JA Golden, T Tao Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein Biosci. Rep., 2020-07-31;40(7):. 2020-07-31 [PMID: 32608477] (Human)
• N Moya, A Shahryari, I Burtscher, J Beckenbaue, M Bakhti, H Lickert Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4) Stem Cell Res, 2020-06-09;46(0):101874. 2020-06-09 [PMID: 32544857] (ICC, Human)
• P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced J Pathol Clin Res, 2019-09-07;5(4):248-255. 2019-09-07 [PMID: 31493350] (IHC-P, Human)
• Lam CJ, Chatterjee A, Shen E et al. Low Level Insulin Content Within Abundant Non-Beta Islet Endocrine Cells in Long-Standing Type 1 Diabetes Diabetes 12/14/2018 [PMID: 30552110]
• Lam CJ, Cox AR, Jacobson DR et al. Highly Proliferative alpha-Cell-Related Islet Endocrine Cells in Human Pancreata. Diabetes 4/1/2018 [PMID: 29326366]

Bioinformatics

• Gene Symbol: ARX
• Uniprot:
  • Q96QS3(Human)