ARX Antibody

Images

 
Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep ...read more
ARX was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room ...read more
ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

ARX Antibody Summary

Immunogen
E. coli-derived recombinant human ARX
Ser2-Ala100
Accession # Q96QS3
Specificity
Detects human ARX in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ARX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARX Antibody

  • aristaless related homeobox
  • Aristaless-related homeobox
  • aristaless-related homeobox, X-linked
  • ARX
  • cancer/testis antigen 121
  • CT121
  • EIEE1
  • homeobox protein ARX
  • ISSX
  • ISSXmental retardation, X-linked 36
  • mental retardation, X-linked 29
  • mental retardation, X-linked 32
  • mental retardation, X-linked 33
  • mental retardation, X-linked 38
  • mental retardation, X-linked 43
  • mental retardation, X-linked 54
  • mental retardation, X-linked 76
  • mental retardation, X-linked 87
  • MRX29
  • MRX32
  • MRX33
  • MRX36
  • MRX38
  • MRX43
  • MRX54
  • MRX76
  • MRX87
  • MRXS1
  • PRTS

Background

ARX (Aristaless-Related homeoboX protein) is a 56-62 kDa member of the aristaless subfamily, paired homeobox family of proteins. In the nervous system, it is expressed in embryonic telencephalon plus adult cortical GABAergic neurons and differentiating neuronal stem cells; in the pancreas, it promotes the differentiation of alpha -cells at the expense of beta -cells. Human ARX is a 562 amino acid (aa) transcriptional repressor. It contains an octapeptide motif that binds Groucho proteins (aa 27‑34), an NLS (aa 82-89), one Ala-rich region (aa 100-155), an acidic/Glu-rich region (aa 224-253), a DNA-binding homeodomain with an embedded NLS (aa 328‑387), one Pro-rich segment that binds CtBP1 (aa 395-459) and a C-terminal Aristaless domain (aa 530-543). There are mutations that show poly-Ala expansions in the N-terminus (aa 115-155), often associated with developmental abnormalities. Over aa 1-100, human ARX shares 97% aa identity with mouse ARX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ARX
Uniprot