Filamin A Antibody (4E1.0-1B2)

Images

 
Western Blot: Filamin A Antibody (4E10-1B2) [H00002316-M01] - FLNA monoclonal antibody (M01), clone 4E10-1B2 Analysis of FLNA expression in HL-60.
Immunohistochemistry-Paraffin: Filamin A Antibody (4E10-1B2) [H00002316-M01] - Analysis of monoclonal antibody to FLNA on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1.5 ug/ml.
Proximity Ligation Assay: Filamin A Antibody (4E10-1B2) [H00002316-M01] - Analysis of protein-protein interactions between GP1BA and FLNA. HeLa cells were stained with anti-GP1BA rabbit purified polyclonal 1:1200 and ...read more
Sandwich ELISA: Filamin A Antibody (4E10-1B2) [H00002316-M01] - Detection limit for recombinant GST tagged FLNA is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P, PLA
Clone
4E1.0-1B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Filamin A Antibody (4E1.0-1B2) Summary

Immunogen
FLNA (AAH14654, 1 a.a. - 838 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPSGKVAQPTITDNKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHVTAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNEQHVPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANVGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGTHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGVYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGNPAEFVVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASKVVAKGLGLSKAYVGQKSSFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHVGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRVVVP
Specificity
FLNA - filamin A, alpha (actin binding protein 280)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FLNA
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Publications
Read Publications using H00002316-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Filamin A Antibody (4E1.0-1B2)

  • ABP-280DKFZp434P031
  • ABPX
  • actin binding protein 280
  • Actin-binding protein 280
  • alpha-filamin
  • CVD1
  • EC 2.1.1.43
  • EC 6.3.4.4
  • Endothelial actin-binding protein
  • filamin A, alpha (actin binding protein 280)
  • filamin A, alpha
  • filamin-1
  • filamin-A
  • FLN
  • FLN1OPD
  • FLN-A
  • FMD
  • MNS
  • NHBP
  • Non-muscle filamin
  • OPD1
  • OPD2
  • XLVD
  • XMVD

Background

Actin-binding protein, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Filamin A Antibody (H00002316-M01)(10)


Showing Publications 1 - 10 of 10.
Publications using H00002316-M01 Applications Species
Mingione A, Verdelli C, Ferrero S et al. Filamin A is reduced and contributes to the CASR sensitivity in human parathyroid tumors. J Mol Endocrinol 2017 Feb [PMID: 27872158]
Gay O, Gilquin B, Assard N et al. Refilins are short-lived Actin-bundling proteins that regulate lamellipodium protrusion dynamics. Biol Open 2016 Oct 15 [PMID: 27744291]
Vitali E, Cambiaghi V, Zerbi A et al. Filamin-A is required to mediate SST2 effects in pancreatic neuroendocrine tumours. Endocr Relat Cancer 2016 Mar [PMID: 26733502]
Bezie S, Picarda E, Tesson L et al. Fibrinogen-Like Protein 2/Fibroleukin Induces Long-Term Allograft Survival in a Rat Model through Regulatory B Cells. PLoS One. 2015 Mar 12 [PMID: 25763980]
Parrini E, Mei D, Pisanti MA et al. Familial periventricular nodular heterotopia, epilepsy and Melnick-Needles Syndrome caused by a single FLNA mutation with combined gain-of-function and loss-of-function effects. J Med Genet. 2015 Mar 19 [PMID: 25755106]
Peverelli E, Giardino E, Treppiedi D et al. Filamin A (FLNA) plays an essential role in somatostatin receptor 2 (SST2) signaling and stabilization after agonist stimulation in human and rat somatotroph tumor cells. Endocrinology. 2014 May 14 [PMID: 24828612]
Sakane A, Alamir Mahmoud Abdallah A, Nakano K et al. Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins. Genes Cells. 2013 Jul 25 [PMID: 23890175]
Oegema R, Hulst JM, Theuns-Valks SD et al. Novel no-stop FLNA mutation causes multi-organ involvement in males. Am J Med Genet A. 2013 Jul 19 [PMID: 23873601]
Peverelli E, Mantovani G, Vitali E et al. Filamin-A Is Essential for Dopamine D2 Receptor Expression and Signaling in Tumorous Lactotrophs. J Clin Endocrinol Metab. 2012 Jan 18 [PMID: 22259062]
Valente EM, Logan CV, Mougou-Zerelli S et al. Mutations in TMEM216 perturb ciliogenesis and cause Joubert, Meckel and related syndromes. Nat Genet;42(7):619-25. 2010 Jul. [PMID: 20512146]

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Bioinformatics

Gene Symbol FLNA
Entrez
OMIM
Uniprot